Name: Tenacibaculum ovolyticum EKD 002
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 5652

Type strain:

Species: Tenacibaculum ovolyticum

Strain Designation: EKD 002

Culture col. no.: CCUG 35199, CIP 106403, DSM 18103, ATCC 51887, IAM 14318, IFO 15947, NCIMB 13127, JCM 21158, LMG 13026, NBRC 15947

Strain history: CIP <- 2000, J.F. Bernardet, INRA, Jouy-en-Josas, France <- G.H. Hansen: strain EKD 002

NCBI tax ID(s): 1123347 (strain), 104270 (species)

SI-ID 13990

LMG 13026, ATCC 51887, CCUG 35199, NCIMB 13127, CIP 106403, IFO 15947, IAM 14318, NBRC 15947, DSM 18103, JCM 21158

Special Collections

Other strains from Tenacibaculum ovolyticum

Section

Tenacibaculum ovolyticum EKD 002 is an aerobe, motile, rod-shaped animal pathogen that was isolated from adherent epiflora of Atlantic Halibut eggs.

motile
rod-shaped
aerobe
animal pathogen
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Flavobacteriaceae*
	Genus	*Tenacibaculum*
	Species	**Tenacibaculum ovolyticum**
	Full Scientific Name (LPSN)	Tenacibaculum ovolyticum (Hansen et al. 1992) Suzuki et al. 2001

Synonym

Flexibacter ovolyticus

Information on morphological and physiological properties Morphology

[Ref.: #121477]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %96

[Ref.: #121477]	Cell shape	rod-shaped
[Ref.: #43375]	Cell shape	rod-shaped

[Ref.: #121477]	Motility	no
[Ref.: #43375]	Motility	yes
[Ref.: #69480]	Motility	no Confidence in %90

[Ref.: #43375]	Cell length	2-20 µm

[Ref.: #43375]	Cell width	0.5 µm

[Ref.: #43375]	Flagellum arrangement	gliding

[Ref.: #43375]

Colony color

pale yellow

[Ref.: #121477]	Pigment production	no
[Ref.: #121477]	Pigment name	Flexirubin

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7396]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #7396]

Culture medium growth

[Ref.: #7396]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7396]

Culture medium composition

expand / minimize

[Ref.: #39511]

Culture medium

Marine agar (MA)

[Ref.: #39511]

Culture medium growth

[Ref.: #39511]

Culture medium composition

expand / minimize

[Ref.: #121477]

Culture medium

CIP Medium 13

[Ref.: #121477]

Culture medium growth

[Ref.: #121477]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #7396]		growth	15 °C
[Ref.: #39511]		growth	15 °C
[Ref.: #43375]		growth	4-25 °C
[Ref.: #52587]		growth	15 °C
[Ref.: #67770]		growth	15 °C
[Ref.: #121477]		growth	5-25 °C
[Ref.: #121477]	no	growth	30 °C

	pH	Kind of pH		pH
[Ref.: #43375]			growth	5.9-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #52587]

Oxygen tolerance

aerobe

[Ref.: #69481]

Ability of spore formation

no Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43375]	2-oxoglutarate ChEBI	-	growth
[Ref.: #43375]	acetate ChEBI	-	growth
[Ref.: #43375]	agar ChEBI	-	degradation
[Ref.: #43375]	aspartate ChEBI	-	growth
[Ref.: #43375]	benzoate ChEBI	-	growth
[Ref.: #43375]	casamino acids	+	growth
[Ref.: #43375]	casamino acids	+/-	growth
[Ref.: #43375]	casein	+	degradation
[Ref.: #43375]	cellulose ChEBI	-	degradation
[Ref.: #43375]	chitin ChEBI	+	degradation
[Ref.: #43375]	citrate ChEBI	-	growth
[Ref.: #43375]	D-ribose ChEBI	-	growth
[Ref.: #43375]	dna ChEBI	+	degradation
[Ref.: #43375]	esculin ChEBI	-	degradation
[Ref.: #121477]	esculin ChEBI	-	hydrolysis
[Ref.: #43375]	ethanol ChEBI	-	growth
[Ref.: #43375]	fumarate ChEBI	-	growth
[Ref.: #43375]	gelatin ChEBI	+	degradation
[Ref.: #43375]	glycerol ChEBI	-	growth
[Ref.: #121477]	hippurate ChEBI	-	hydrolysis
[Ref.: #43375]	L-glutamate ChEBI	-	growth
[Ref.: #43375]	L-leucine ChEBI	-	growth
[Ref.: #43375]	L-proline ChEBI	-	growth
[Ref.: #43375]	L-tartrate ChEBI	-	growth
[Ref.: #43375]	malate ChEBI	-	growth
[Ref.: #43375]	methanol ChEBI	-	growth
[Ref.: #43375]	N-acetylglucosamine ChEBI	-	growth
[Ref.: #43375]	nitrate ChEBI	+	reduction
[Ref.: #121477]	nitrate ChEBI	+	reduction
[Ref.: #121477]	nitrite ChEBI	-	reduction
[Ref.: #43375]	propionate ChEBI	-	growth
[Ref.: #43375]	pyruvate ChEBI	-	growth
[Ref.: #43375]	starch ChEBI	-	degradation
[Ref.: #43375]	succinate ChEBI	-	growth
[Ref.: #43375]	sucrose ChEBI	-	growth
[Ref.: #43375]	tryptone	+	growth
[Ref.: #43375]	tween 80 ChEBI	+	degradation
[Ref.: #43375]	tyrosine ChEBI	+	degradation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #121477]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121477]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121477]	amylase	-
[Ref.: #121477]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121477]	caseinase	+	3.4.21.50
[Ref.: #43375]	catalase	+	1.11.1.6
[Ref.: #121477]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #43375]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #121477]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #121477]	gelatinase	+
[Ref.: #121477]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #121477]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121477]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121477]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #121477]	oxidase	+
[Ref.: #121477]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #121477]	protease	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121477]	tryptophan deaminase	-
[Ref.: #121477]	tween esterase	+
[Ref.: #121477]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121477]	9471	-	+	+	+	-	+	+	+	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7396]	Sample type/isolated from	adherent epiflora of Atlantic Halibut (Hippoglossus hippoglossus L) eggs
[Ref.: #7396]	Country	Norway
[Ref.: #7396]	Country ISO 3 Code	NOR
[Ref.: #7396]	Continent	Europe

[Ref.: #52587]	Sample type/isolated from	Halibut,adherent epiflora of Atlantic halibut eggs

[Ref.: #67770]	Sample type/isolated from	Adherent epiflora of Atlantic halibut (Hippoglossus hippoglossus) eggs
[Ref.: #67770]	Host species	Hippoglossus hippoglossus
[Ref.: #67770]	Country	Norway
[Ref.: #67770]	Country ISO 3 Code	NOR
[Ref.: #67770]	Continent	Europe

[Ref.: #121477]	Sample type/isolated from	Atlantic halibut, Hippoglossus hipoglossus, eggs
[Ref.: #121477]	Country	Norway
[Ref.: #121477]	Country ISO 3 Code	NOR
[Ref.: #121477]	Continent	Europe
[Ref.: #121477]	Isolation date	1989
* marker position based on {}

Isolation sources categories

#Host	#Fishes	-
#Host Body Product	#Urogenital tract	#Egg

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB032506 (>99% sequence identity) for Tenacibaculum ovolyticum subclade from Microbeatlas

Aquatic Samples	1136
Soil Samples	22
Animal Samples	152
Plant Samples
Total Samples	1310

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7396]	Pathogenicity (animal)	yes
[Ref.: #7396]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121477]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7396]	Tenacibaculum ovolyticum gene for 16S rRNA, strain:IFO 15947	AB078058	1473	GenBank ENA	104270 tax ID
[Ref.: #124043]	Tenacibaculum ovolyticum gene for 16S rRNA, partial sequence, strain: NBRC 15947.	AB681005	1443	GenBank ENA	104270 tax ID	*
[Ref.: #20218]	Tenacibaculum ovolyticus gene for 16S rRNA, partial sequence, strain:IAM14318	AB032506	1440	GenBank ENA	104270 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Tenacibaculum ovolyticum DSM 18103	GCA_000430545	scaffold	GenBank ENA	1123347 tax ID
[Ref.: #66792]	Tenacibaculum ovolyticum DSM 18103	1123347.4	wgs	PATRIC	1123347 tax ID	*
[Ref.: #66792]	Tenacibaculum ovolyticum DSM 18103	2523533531	draft	JGI IMG/M	1123347 tax ID	*

[Ref.: #43375]

GC-content

30.3-32 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Tenacibaculum ovolyticum DSM 18103 DSM 18103 NCBI: GCA_000430545.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Tenacibaculum ovolyticum DSM 18103 PATRIC: 1123347.4
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	96	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	96.849	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	85.2	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	84.505	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.972	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	90	yes

Availability in culture collections External links

[Ref.: #7396]

Culture collection no.

CCUG 35199, CIP 106403, DSM 18103, ATCC 51887, IAM 14318, IFO 15947, NCIMB 13127, JCM 21158, LMG 13026, NBRC 15947

[Ref.: #75135]

SI-ID 13990

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genome Sequence of the Psychrophilic Bacterium Tenacibaculum ovolyticum Strain da5A-8 Isolated from Deep Seawater.	Teramoto M, Zhai Z, Komatsu A, Shibayama K, Suzuki M	Genome Announc	10.1128/genomeA.00644-16	2016	*
Phylogeny	Tenacibaculum finnmarkense sp. nov., a fish pathogenic bacterium of the family Flavobacteriaceae isolated from Atlantic salmon.	Smage SB, Brevik OJ, Duesund H, Ottem KF, Watanabe K, Nylund A	Antonie Van Leeuwenhoek	10.1007/s10482-015-0630-0	2015	*
Phylogeny	Flexibacter ovolyticus sp. nov., a pathogen of eggs and larvae of Atlantic halibut, Hippoglossus hippoglossus L.	Hansen GH, Bergh O, Michaelsen J, Knappskog D	Int J Syst Bacteriol	10.1099/00207713-42-3-451	1992	*

References

#7396

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18103

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39511 ; Curators of the CIP;

#43375

Makoto Suzuki, Yasuyoshi Nakagawa, Shigeaki Harayama, Satoshi Yamamoto: Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria : proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov.. IJSEM 51: 1639 - 1652 2001 ( DOI 10.1099/00207713-51-5-1639 , PubMed 11594591 )

#52587 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 35199

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#75135

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID13990.1 )

#121477 Collection of Institut Pasteur ; Curators of the CIP; CIP 106403

#124043

Dr. Isabel Schober, Dr. Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

* These data were automatically processed and therefore are not curated

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Tenacibaculum ovolyticum DSM 18103 DSM 18103

Tenacibaculum ovolyticum DSM 18103

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