Anaerolinea thermophila (DSM 14523, JCM 11388, UNI-1)

Name: Anaerolinea thermophila (DSM 14523, JCM 11388, UNI-1)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 501

Type strain

Strain Designation UNI-1

Culture col. no. DSM 14523 JCM 11388 UNI-1

NCBI tax ID(s) 167964

Special Collections DSMZ JCM Literature strains

History DSMZ <- Y. Sekiguchi, AIST; UNI-1 <- H. Takahashi and Y. Sekiguchi {2000}

Links

Anaerolinea thermophila UNI-1 is an anaerobe, Gram-negative, filament-shaped bacterium that was isolated from granular sludge in a thermophilic upflow anaerobic sludge blanket reactor treating soybean-curd manufacturing waste water.

Gram-negative filament-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 265481

DSM 14523,JCM 11388,NBRC 100420

@ref 20215

Last LPSN update 2026-05-29 08:25:02

Domain Bacteria

Phylum Chloroflexota

Class Anaerolineae

Order Anaerolineales

Family Anaerolineaceae

Genus Anaerolinea

Species Anaerolinea thermophila

Full scientific name Anaerolinea thermophila Sekiguchi et al. 2003

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
23127	negative	>100 µm	0.2-0.3 µm	filament-shaped

Colony morphology

23127

Medium usedyeast extract

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
5283	ANAEROLINEA MEDIUM (DSMZ Medium 1004)		Medium recipe at MediaDive	Name: ANAEROLINEA MEDIUM (DSMZ Medium 1004) Composition: NaHCO3 2.49252 g/l Yeast extract 2.29312 g/l D-Glucose 2.19342 g/l NH4Cl 0.538385 g/l Na2S x 9 H2O 0.249252 g/l L-Cysteine HCl x H2O 0.249252 g/l MgCl2 x 6 H2O 0.199402 g/l CaCl2 x 2 H2O 0.149551 g/l KH2PO4 0.139581 g/l Na2-EDTA x 2 H2O 0.00518445 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water
23127	yeast extract

Culture temp

@ref	Growth	Type	Temperature (°C)
5283	positive	growth	55
23127	positive	maximum	60
23127	positive	minimum	55
23127	positive	optimum	55

Culture pH

@ref	Ability	Type	PH
23127	positive	maximum	8.0
23127	positive	minimum	6.0
23127	positive	optimum	7.0

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
5283	anaerobe
23127	anaerobe
125439	facultative anaerobe	90.097

Spore formation

@ref	Spore formation	Confidence
23127
125439		94.395

Halophily

@ref	Salt	Growth	Tested relation	Concentration
23127	NaCl		growth	<5 g/L

Metabolite utilization

@ref	Metabolite	Utilization activity	Kind of utilization tested
23127	yeast extract	+	required for growth

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Waste	#Activated sludge
#Condition	#Anoxic (anaerobic)	-
#Engineered	#Biodegradation	#Anaerobic digestor
#Engineered	#Food production	-
#Engineered	#Waste	#Wastewater
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
5283	granular sludge in a thermophilic upflow anaerobic sludge blanket reactor treating soybean-curd manufacturing waste water	Japan	JPN	Asia
23127	thermophilic anaerobic granular sludge blanket reactor treating soybean-curd manufacturing waste water

Taxonmaps

69479

Global distribution of 16S sequence AB046413 (>99% sequence identity) for Anaerolinea thermophila subclade from Microbeatlas

Aquatic Samples	933
Soil Samples	181
Animal Samples	251
Plant Samples	33
Total Samples	1398

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5283	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM19967v1 assembly for Anaerolinea thermophila UNI-1	complete	GCA_000199675	926569.3	649633005	926569	96.87

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
5283	Anaerolinea thermophila gene for 16S ribosomal RNA, partial sequence	AB046413	1457		167964

GC content

@ref	GC-content (mol%)	Method
5283	54.5
23127	54.5	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM19967v1 assembly for Anaerolinea thermophila UNI-1
BV-BRC: 926569.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	90.10	no
125439	gram_stain	BacteriaNetⓘ	negative	58.25	no
125439	motility	BacteriaNetⓘ	no	71.16	no
125439	spore_formation	BacteriaNetⓘ	no	94.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Anaerolinea thermophila UNI-1 UNI-1
NCBI: GCA_000199675.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	58.34	no
125438	anaerobic	anaerobicⓘ	yes	78.70	yes
125438	aerobic	aerobicⓘ	no	66.67	no
125438	spore-forming	spore-formingⓘ	no	70.44	no
125438	thermophilic	thermophileⓘ	no	52.74	yes
125438	flagellated	motile2+ⓘ	no	80.00	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
	Current status of the novel food ingredient safety evaluation system.	Heo S, Lee G, Na HE, Park JH, Kim T, Oh SE, Jeong DW.	Food Sci Biotechnol	10.1007/s10068-023-01396-w	2024
Metabolism	Complex coupled metabolic and prokaryotic community responses to increasing temperatures in anaerobic marine sediments: critical temperatures and substrate changes.	Roussel EG, Cragg BA, Webster G, Sass H, Tang X, Williams AS, Gorra R, Weightman AJ, Parkes RJ.	FEMS Microbiol Ecol	10.1093/femsec/fiv084	2015
	Archaeal community diversity and abundance changes along a natural salinity gradient in estuarine sediments.	Webster G, O'Sullivan LA, Meng Y, Williams AS, Sass AM, Watkins AJ, Parkes RJ, Weightman AJ.	FEMS Microbiol Ecol	10.1093/femsec/fiu025	2015
Phylogeny	Anaerolinea thermophila gen. nov., sp. nov. and Caldilinea aerophila gen. nov., sp. nov., novel filamentous thermophiles that represent a previously uncultured lineage of the domain Bacteria at the subphylum level.	Sekiguchi Y, Yamada T, Hanada S, Ohashi A, Harada H, Kamagata Y	Int J Syst Evol Microbiol	10.1099/ijs.0.02699-0	2003

References

#5283	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14523
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23127	Yuji Sekiguchi, Takeshi Yamada, Satoshi Hanada, Akiyoshi Ohashi, Hideki Harada, Yoichi Kamagata: Anaerolinea thermophila gen. nov., sp. nov. and Caldilinea aerophila gen. nov., sp. nov., novel filamentous thermophiles that represent a previously uncultured lineage of the domain Bacteria at the subphylum level. IJSEM 53: 1843 - 1851 2003 ( DOI 10.1099/ijs.0.02699-0 , PubMed 14657113 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Anaerolinea thermophila (DSM 14523, JCM 11388, UNI-1)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000199675

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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