Name: Anaerolinea thermophila UNI-1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 501

Type strain:

Species: Anaerolinea thermophila

Strain Designation: UNI-1

Culture col. no.: DSM 14523, JCM 11388

Strain history: <- Y. Sekiguchi, AIST; UNI-1 <- H. Takahashi and Y. Sekiguchi {2000}

NCBI tax ID(s): 167964 (species)

SI-ID 265481

DSM 14523, JCM 11388, NBRC 100420

Special Collections

Section

Anaerolinea thermophila UNI-1 is an anaerobe, Gram-negative, filament-shaped bacterium that was isolated from granular sludge in a thermophilic upflow anaerobic sludge blanket reactor treating soybean-curd manufacturing waste water.

filament-shaped
Gram-negative
anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Chloroflexota*
	Class	*Anaerolineae*
	Order	*Anaerolineales*
	Family	*Anaerolineaceae*
	Genus	*Anaerolinea*
	Species	**Anaerolinea thermophila**
	Full Scientific Name (LPSN)	Anaerolinea thermophila Sekiguchi et al. 2003

Information on morphological and physiological properties Morphology

[Ref.: #23127]	Gram stain	negative

[Ref.: #23127]	Cell length	>100 µm

[Ref.: #23127]	Cell width	0.2-0.3 µm

[Ref.: #23127]	Cell shape	filament-shaped

[Ref.: #23127]	Motility	no

[Ref.: #23127]

Cultivation medium used

yeast extract

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5283]

Culture medium

ANAEROLINEA MEDIUM (DSMZ Medium 1004)

[Ref.: #5283]

Culture medium growth

[Ref.: #5283]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5283]

Culture medium composition

expand / minimize

[Ref.: #23127]

Culture medium

yeast extract

[Ref.: #23127]

Culture medium growth

		Temperature
[Ref.: #5283]	growth	55 °C
[Ref.: #23127]	maximum	60 °C
[Ref.: #23127]	minimum	55 °C
[Ref.: #23127]	optimum	55 °C

		pH
[Ref.: #23127]	maximum	8.0
[Ref.: #23127]	minimum	6.0
[Ref.: #23127]	optimum	7.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5283]	Oxygen tolerance	anaerobe
[Ref.: #23127]	Oxygen tolerance	anaerobe

[Ref.: #23127]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23127]		NaCl	no	growth	<5	g/L

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23127]		yeast extract	+	required for growth

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5283]	Sample type/isolated from	granular sludge in a thermophilic upflow anaerobic sludge blanket reactor treating soybean-curd manufacturing waste water
[Ref.: #5283]	Country	Japan
[Ref.: #5283]	Country ISO 3 Code	JPN
[Ref.: #5283]	Continent	Asia

[Ref.: #23127]	Sample type/isolated from	thermophilic anaerobic granular sludge blanket reactor treating soybean-curd manufacturing waste water
* marker position based on {}

Isolation sources categories

#Engineered	#Waste	#Activated sludge
#Condition	#Anoxic (anaerobic)	-
#Engineered	#Biodegradation	#Anaerobic digestor
#Engineered	#Food production	-
#Engineered	#Waste	#Wastewater
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB046413 (>99% sequence identity) for Anaerolinea thermophila subclade from Microbeatlas

Aquatic Samples	933
Soil Samples	181
Animal Samples	251
Plant Samples	33
Total Samples	1398

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5283]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5283]	Anaerolinea thermophila gene for 16S ribosomal RNA, partial sequence	AB046413	1457	GenBank ENA	167964 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Anaerolinea thermophila UNI-1	GCA_000199675	complete	GenBank ENA	926569 tax ID	*
[Ref.: #66792]	Anaerolinea thermophila UNI-1	926569.3	complete	PATRIC	926569 tax ID	*
[Ref.: #66792]	Anaerolinea thermophila UNI-1	649633005	complete	JGI IMG/M	926569 tax ID	*

[Ref.: #5283]	GC-content	54.5 mol%
[Ref.: #23127]	GC-content	54.5 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Anaerolinea thermophila UNI-1 UNI-1 NCBI: GCA_000199675.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	58.34	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	78.703	yes
[Ref.: #69480]	aerobic	aerobicⓘ	no	66.674	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	70.444	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	52.742	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	80	yes

Availability in culture collections External links

[Ref.: #5283]

Culture collection no.

DSM 14523, JCM 11388

[Ref.: #70180]

SI-ID 265481

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Anaerolinea thermophila gen. nov., sp. nov. and Caldilinea aerophila gen. nov., sp. nov., novel filamentous thermophiles that represent a previously uncultured lineage of the domain Bacteria at the subphylum level.	Sekiguchi Y, Yamada T, Hanada S, Ohashi A, Harada H, Kamagata Y	Int J Syst Evol Microbiol	10.1099/ijs.0.02699-0	2003	*

References

#5283

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14523

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23127

Yuji Sekiguchi, Takeshi Yamada, Satoshi Hanada, Akiyoshi Ohashi, Hideki Harada, Yoichi Kamagata: Anaerolinea thermophila gen. nov., sp. nov. and Caldilinea aerophila gen. nov., sp. nov., novel filamentous thermophiles that represent a previously uncultured lineage of the domain Bacteria at the subphylum level. IJSEM 53: 1843 - 1851 2003 ( DOI 10.1099/ijs.0.02699-0 , PubMed 14657113 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#70180

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID265481.1 )

* These data were automatically processed and therefore are not curated

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Anaerolinea thermophila UNI-1 UNI-1

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