Strain identifier
BacDive ID: 501
Type strain: 
Species: Anaerolinea thermophila
Strain Designation: UNI-1
Strain history: <- Y. Sekiguchi, AIST; UNI-1 <- H. Takahashi and Y. Sekiguchi {2000}
NCBI tax ID(s): 167964 (species)
version 8.1 (current version)
General
@ref: 5283
BacDive-ID: 501
DSM-Number: 14523
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-negative, filament-shaped
description: Anaerolinea thermophila UNI-1 is an anaerobe, thermophilic, Gram-negative bacterium that was isolated from granular sludge in a thermophilic upflow anaerobic sludge blanket reactor treating soybean-curd manufacturing waste water.
NCBI tax id
- NCBI tax id: 167964
- Matching level: species
strain history
- @ref: 5283
- history: <- Y. Sekiguchi, AIST; UNI-1 <- H. Takahashi and Y. Sekiguchi {2000}
doi: 10.13145/bacdive501.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/chloroflexota
- domain: Bacteria
- phylum: Chloroflexota
- class: Anaerolineae
- order: Anaerolineales
- family: Anaerolineaceae
- genus: Anaerolinea
- species: Anaerolinea thermophila
- full scientific name: Anaerolinea thermophila Sekiguchi et al. 2003
@ref: 5283
domain: Bacteria
phylum: Chloroflexi
class: Anaerolineae
order: Anaerolineales
family: Anaerolineaceae
genus: Anaerolinea
species: Anaerolinea thermophila
full scientific name: Anaerolinea thermophila Sekiguchi et al. 2003
strain designation: UNI-1
type strain: yes
Morphology
cell morphology
- @ref: 23127
- gram stain: negative
- cell length: >100 µm
- cell width: 0.2-0.3 µm
- cell shape: filament-shaped
- motility: no
colony morphology
- @ref: 23127
- medium used: yeast extract
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 5283 | ANAEROLINEA MEDIUM (DSMZ Medium 1004) | yes | https://mediadive.dsmz.de/medium/1004 | Name: ANAEROLINEA MEDIUM (DSMZ Medium 1004) Composition: NaHCO3 2.49252 g/l Yeast extract 2.29312 g/l D-Glucose 2.19342 g/l NH4Cl 0.538385 g/l Na2S x 9 H2O 0.249252 g/l L-Cysteine HCl x H2O 0.249252 g/l MgCl2 x 6 H2O 0.199402 g/l CaCl2 x 2 H2O 0.149551 g/l KH2PO4 0.139581 g/l Na2-EDTA x 2 H2O 0.00518445 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water |
| 23127 | yeast extract | yes |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 5283 | positive | growth | 55 | thermophilic |
| 23127 | positive | maximum | 60 | thermophilic |
| 23127 | positive | minimum | 55 | thermophilic |
| 23127 | positive | optimum | 55 | thermophilic |
culture pH
| @ref | ability | type | pH |
|---|---|---|---|
| 23127 | positive | minimum | 6.0 |
| 23127 | positive | maximum | 8.0 |
| 23127 | positive | optimum | 7.0 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 5283 | anaerobe |
| 23127 | anaerobe |
spore formation
- @ref: 23127
- spore formation: no
halophily
- @ref: 23127
- salt: NaCl
- growth: no
- tested relation: growth
- concentration: <5 g/L
metabolite utilization
- @ref: 23127
- metabolite: yeast extract
- utilization activity: +
- kind of utilization tested: required for growth
Isolation, sampling and environmental information
isolation
| @ref | sample type | country | origin.country | continent |
|---|---|---|---|---|
| 5283 | granular sludge in a thermophilic upflow anaerobic sludge blanket reactor treating soybean-curd manufacturing waste water | Japan | JPN | Asia |
| 23127 | thermophilic anaerobic granular sludge blanket reactor treating soybean-curd manufacturing waste water |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Waste | #Activated sludge |
| #Condition | #Anoxic (anaerobic) | |
| #Engineered | #Biodegradation | #Anaerobic digestor |
| #Engineered | #Food production | |
| #Engineered | #Waste | #Wastewater |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_3455.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_405;96_1805;97_2163;98_2654;99_3455&stattab=map
- Last taxonomy: Anaerolinea thermophila subclade
- 16S sequence: AB046413
- Sequence Identity:
- Total samples: 1398
- soil counts: 181
- aquatic counts: 933
- animal counts: 251
- plant counts: 33
Safety information
risk assessment
- @ref: 5283
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 5283
- description: Anaerolinea thermophila gene for 16S ribosomal RNA, partial sequence
- accession: AB046413
- length: 1457
- database: ena
- NCBI tax ID: 167964
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Anaerolinea thermophila UNI-1 | GCA_000199675 | complete | ncbi | 926569 |
| 66792 | Anaerolinea thermophila UNI-1 | 926569.3 | complete | patric | 926569 |
| 66792 | Anaerolinea thermophila UNI-1 | 649633005 | complete | img | 926569 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 5283 | 54.5 | |
| 23127 | 54.5 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 90.092 | yes |
| flagellated | no | 96.561 | yes |
| gram-positive | no | 93.803 | yes |
| anaerobic | yes | 84.753 | no |
| aerobic | no | 93.148 | no |
| halophile | no | 94.845 | no |
| spore-forming | no | 91.675 | yes |
| thermophile | yes | 99.471 | yes |
| glucose-util | yes | 80.821 | no |
| glucose-ferment | no | 73.376 | no |
External links
@ref: 5283
culture collection no.: DSM 14523, JCM 11388
straininfo link
- @ref: 70180
- straininfo: 265481
literature
- topic: Phylogeny
- Pubmed-ID: 14657113
- title: Anaerolinea thermophila gen. nov., sp. nov. and Caldilinea aerophila gen. nov., sp. nov., novel filamentous thermophiles that represent a previously uncultured lineage of the domain Bacteria at the subphylum level.
- authors: Sekiguchi Y, Yamada T, Hanada S, Ohashi A, Harada H, Kamagata Y
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.02699-0
- year: 2003
- mesh: Bacteria/classification, Chloroflexi/*classification/*genetics/growth & development/ultrastructure, Microscopy, Electron, Molecular Sequence Data, *Phylogeny
- topic2: Genetics
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 5283 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14523) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-14523 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 23127 | Yuji Sekiguchi, Takeshi Yamada, Satoshi Hanada, Akiyoshi Ohashi, Hideki Harada, Yoichi Kamagata | 10.1099/ijs.0.02699-0 | Anaerolinea thermophila gen. nov., sp. nov. and Caldilinea aerophila gen. nov., sp. nov., novel filamentous thermophiles that represent a previously uncultured lineage of the domain Bacteria at the subphylum level | IJSEM 53: 1843-1851 2003 | 14657113 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 70180 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID265481.1 | StrainInfo: A central database for resolving microbial strain identifiers |