Name: Anaerolinea thermolimosa IMO-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 502

Type strain:

Species: Anaerolinea thermolimosa

Strain Designation: IMO-1

Culture col. no.: DSM 16554, JCM 12577

Strain history: T. Yamada IMO-1.

NCBI tax ID(s): 229919 (species)

SI-ID 297125

DSM 16554, JCM 12577

Section

Anaerolinea thermolimosa IMO-1 is an anaerobe, thermophilic, Gram-negative bacterium that was isolated from sludge from a thermophilic UASB reactor.

filament-shaped
Gram-negative
anaerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Chloroflexota*
	Class	*Anaerolineae*
	Order	*Anaerolineales*
	Family	*Anaerolineaceae*
	Genus	*Anaerolinea*
	Species	**Anaerolinea thermolimosa**
	Full Scientific Name (LPSN)	Anaerolinea thermolimosa Yamada et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #23356]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.579

[Ref.: #23356]	Cell length	>100 µm

[Ref.: #23356]	Cell width	0.3-0.4 µm

[Ref.: #23356]	Cell shape	filament-shaped

[Ref.: #23356]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6506]

Culture medium

ANAEROLINEA MEDIUM (DSMZ Medium 1004)

[Ref.: #6506]

Culture medium growth

[Ref.: #6506]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6506]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #6506]	growth	50 °C
[Ref.: #23356]	maximum	55 °C
[Ref.: #23356]	minimum	42 °C
[Ref.: #23356]	optimum	50 °C
[Ref.: #67770]	growth	50 °C

[Ref.: #6506]	Temperature range	thermophilic
[Ref.: #23356]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic
[Ref.: #69480]	Temperature range	thermophilic Confidence in %96.162

		pH
[Ref.: #23356]	maximum	7.5
[Ref.: #23356]	minimum	6.0
[Ref.: #23356]	optimum	7.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #6506]	Oxygen tolerance	anaerobe
[Ref.: #23356]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %93.398

[Ref.: #69481]	Ability of spore formation	no Confidence in %94
[Ref.: #69480]	Ability of spore formation	no Confidence in %98.804

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23356]		yeast extract	+	required for growth

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6506]	Sample type/isolated from	sludge from a thermophilic UASB reactor
[Ref.: #6506]	Geographic location (country and/or sea, region)	Niigata, Nagaoka
[Ref.: #6506]	Country	Japan
[Ref.: #6506]	Country ISO 3 Code	JPN
[Ref.: #6506]	Continent	Asia

[Ref.: #23356]	Sample type/isolated from	sludge from a thermophilic UASB reactor, in wastewater from manufacture of a Japanese distilled alcohol

[Ref.: #67770]	Sample type/isolated from	Granular sludge collected from a UASB reactor operated under a thermophilic condition (55C)
* marker position based on {}

Isolation sources categories

#Engineered	#Biodegradation	#Anaerobic digestor
#Engineered	#Waste	#Wastewater
#Environmental	#Terrestrial	#Mud (Sludge)
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB109437 (>99% sequence identity) for Anaerolinea thermolimosa subclade from Microbeatlas

Aquatic Samples	434
Soil Samples	137
Animal Samples	320
Plant Samples	25
Total Samples	916

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6506]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6506]	Anaerolinea thermolimosa gene for 16S rRNA, partial sequence	AB109437	1428	ENA	229919 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Anaerolinea thermolimosa IMO-1	GCA_001050195	scaffold	GenBank	229919 tax ID
[Ref.: #66792]	Anaerolinea thermolimosa strain IMO-1	229919.9	wgs	PATRIC	229919 tax ID	*

[Ref.: #6506]	GC-content	53.3 mol%
[Ref.: #23356]	GC-content	53.3 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	54.6 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Anaerolinea thermolimosa IMO-1 NCBI: GCA_001050195.2
[Ref.: #69481]	spore-forming	no	94	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Anaerolinea thermolimosa strain IMO-1 PATRIC: 229919.9
[Ref.: #69480]	motile	no	56.075	no
[Ref.: #69480]	gram-positive	no	95.387	yes
[Ref.: #69480]	anaerobic	yes	70.848	no
[Ref.: #69480]	aerobic	no	92.273	yes
[Ref.: #69480]	halophile	no	95.879	no
[Ref.: #69480]	spore-forming	no	82.816	no
[Ref.: #69480]	glucose-util	yes	85.091	no
[Ref.: #69480]	flagellated	no	89.917	no
[Ref.: #69480]	thermophile	yes	98.119	yes
[Ref.: #69480]	glucose-ferment	no	75.153	no

Availability in culture collections External links

[Ref.: #6506]

Culture collection no.

DSM 16554, JCM 12577

[Ref.: #70181]

SI-ID 297125

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft Genome Sequences of Anaerolinea thermolimosa IMO-1, Bellilinea caldifistulae GOMI-1, Leptolinea tardivitalis YMTK-2, Levilinea saccharolytica KIBI-1, Longilinea arvoryzae KOME-1, Previously Described as Members of the Class Anaerolineae (Chloroflexi).	Matsuura N, Tourlousse DM, Ohashi A, Hugenholtz P, Sekiguchi Y	Genome Announc	10.1128/genomeA.00975-15	2015	*
Phylogeny	Anaerolinea thermolimosa sp. nov., Levilinea saccharolytica gen. nov., sp. nov. and Leptolinea tardivitalis gen. nov., sp. nov., novel filamentous anaerobes, and description of the new classes Anaerolineae classis nov. and Caldilineae classis nov. in the bacterial phylum Chloroflexi.	Yamada T, Sekiguchi Y, Hanada S, Imachi H, Ohashi A, Harada H, Kamagata Y	Int J Syst Evol Microbiol	10.1099/ijs.0.64169-0	2006	*

References

#6506

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16554

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23356

Takeshi Yamada, Yuji Sekiguchi, Satoshi Hanada, Hiroyuki Imachi, Akiyoshi Ohashi, Hideki Harada, Yoichi Kamagata: Anaerolinea thermolimosa sp. nov., Levilinea saccharolytica gen. nov., sp. nov. and Leptolinea tardivitalis gen. nov., sp. nov., novel filamentous anaerobes, and description of the new classes Anaerolineae classis nov. and Caldilineae classis nov. in the bacterial phylum Chloroflexi. IJSEM 56: 1331 - 1340 2006 ( DOI 10.1099/ijs.0.64169-0 , PubMed 16738111 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70181

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID297125.1 )

* These data were automatically processed and therefore are not curated

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Anaerolinea thermolimosa IMO-1

Anaerolinea thermolimosa strain IMO-1

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