Effusibacillus pohliae (DSM 22757, CIP 109385, NCIMB 14276)

Name: Effusibacillus pohliae (DSM 22757, CIP 109385, NCIMB 14276)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 434

Type strain

Strain Designation MP4

Culture col. no. DSM 22757 CIP 109385 NCIMB 14276 MP4

NCBI tax ID(s) 1120973 232270

Special Collections DSMZ CIP Literature strains

History 2006, L. Marri, Siena Univ., Sienna, Italy, "Alicyclobacillus melbournensis": strain MP4 <- CIP <- L. Marri, Univ. Siena, Italy; MP4 (Alicyclobacillus melbournensis) CIP <- 2006, L. Marri, Siena Univ., Siena, Italy, "Alicyclobacillus melbournensis": strain MP4

Links

Effusibacillus pohliae MP4 is an aerobe, spore-forming, thermophilic prokaryote that was isolated from geothermal soil .

spore-forming Gram-positive rod-shaped aerobe thermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 323943

CIP 109385,NCIMB 14276,DSM 22757

@ref 20215

Last LPSN update 2025-12-16 09:46:51

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Alicyclobacillaceae

Genus Effusibacillus

Species Effusibacillus pohliae

Full scientific name Effusibacillus pohliae (Imperio et al. 2008) Watanabe et al. 2014

Synonyms (1)

Alicyclobacillus pohliae

BacDive ID	Other strains from **Effusibacillus pohliae** (3)	Type strain
100603	E. pohliae SF006210(FSU),
100604	E. pohliae SF002674(FSU),
100605	E. pohliae SF002675(FSU),

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
32186	positive	2 µm	0.5 µm	rod-shaped
121921				rod-shaped
125439					93.3

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
16497	ALICYCLOBACILLUS POHLIAE MEDIUM (DSMZ Medium 1209)	Medium recipe at MediaDive	Name: ALICYCOBACILLUS POHLIAE MEDIUM (DSMZ Medium 1209) Composition: Agar 15.0 g/l Tryptone 5.0 g/l D-Glucose 0.5 g/l (NH4)2SO4 0.1 g/l FeSO4 x 7 H2O 0.07 g/l Distilled water
36573	MEDIUM 685 - for Alicyclobacillus melbournensis
121921	CIP Medium 685	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
16497	positive	growth	55	thermophilic
32186	positive	growth	42-60	thermophilic
32186	positive	optimum	55	thermophilic
36573	positive	growth	55	thermophilic

Culture pH

@ref	Ability	Type	PH
32186	positive	optimum	5.5
32186	positive	growth	4.5-7.5

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
32186	aerobe
121921	obligate aerobe
125439	aerobe	95.9

Spore formation

@ref	Spore formation	Confidence
32186
125438		93.58
125439		95.2

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
32186	16808 ChEBI	2-dehydro-D-gluconate	+	carbon source
32186	58143 ChEBI	5-dehydro-D-gluconate	+	carbon source
32186	22599 ChEBI	arabinose	+	carbon source
32186	17057 ChEBI	cellobiose	+	carbon source
32186	28757 ChEBI	fructose	+	carbon source
32186	33984 ChEBI	fucose	+	carbon source
32186	28260 ChEBI	galactose	+	carbon source
32186	17234 ChEBI	glucose	+	carbon source
32186	28087 ChEBI	glycogen	+	carbon source
32186	17716 ChEBI	lactose	+	carbon source
32186	17306 ChEBI	maltose	+	carbon source
32186	37684 ChEBI	mannose	+	carbon source
32186	28053 ChEBI	melibiose	+	carbon source
32186	506227 ChEBI	N-acetylglucosamine	+	carbon source
121921	17632 ChEBI	nitrate	+	reduction
121921	16301 ChEBI	nitrite	-	reduction
32186	26546 ChEBI	rhamnose	+	carbon source
32186	33942 ChEBI	ribose	+	carbon source
32186	17992 ChEBI	sucrose	+	carbon source
32186	18222 ChEBI	xylose	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
121921	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
121921	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121921	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
121921	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121921	-	-	+	+	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date	Latitude	Longitude
16497	geothermal soil (in the immediate vicinity of a steam vent)	northern Victoria Land, north-west slope of Mount Melbourne (74°20.994'S 164°39.792'E, 2498 m altitude)			Australia and Oceania		-74.3499	164.663 -74.3499/164.663
121921	Environment, Geothermal soil	Mount Melbourne, Victoria land	Antarctica	ATA	Antarctica	2002

Taxonmaps

69479

Global distribution of 16S sequence AJ564766 (>99% sequence identity) for Effusibacillus pohliae subclade from Microbeatlas

Aquatic Samples	155
Soil Samples	132
Animal Samples	1191
Plant Samples	31
Total Samples	1509

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
16497	1	Risk group (German classification) According to TRBA 466
121921	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM37622v1 assembly for Effusibacillus pohliae DSM 22757	contig	GCA_000376225	1120973.4	2518645625	1120973	47.67

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
16497	Alicyclobacillus pohliae partial 16S rRNA gene, type strain CIP 109385T	AJ564766	1505		232270

GC content

16497

GC-content (mol%)55.1

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Effusibacillus pohliae DSM 22757
NCBI: GCA_000376225

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	95.20	no
125439	motility	BacteriaNetⓘ	yes	93.30	no
125439	gram_stain	BacteriaNetⓘ	variable	86.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	95.90	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Alicyclobacillus pohliae DSM 22757
PATRIC: 1120973.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	73.75	yes
125438	anaerobic	anaerobicⓘ	no	86.17	no
125438	spore-forming	spore-formingⓘ	yes	93.58	yes
125438	aerobic	aerobicⓘ	yes	80.08	yes
125438	thermophilic	thermophileⓘ	yes	59.79	no
125438	flagellated	motile2+ⓘ	yes	78.21	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Metal Ion Supplementation to Boost Melanin Production by Streptomyces nashvillensis.	Restaino OF, Kordjazi T, Tancredi F, Manini P, Lanzillo F, Raganati F, Marzocchella A, Porta R, Mariniello L.	Int J Mol Sci	10.3390/ijms26010416	2025
	Biotechnological Production and Characterization of Extracellular Melanin by Streptomyces nashvillensis.	Restaino OF, Manini P, Kordjazi T, Alfieri ML, Rippa M, Mariniello L, Porta R.	Microorganisms	10.3390/microorganisms12020297	2024
	Streptomycetes as Microbial Cell Factories for the Biotechnological Production of Melanin.	Kordjazi T, Mariniello L, Giosafatto CVL, Porta R, Restaino OF.	Int J Mol Sci	10.3390/ijms25053013	2024
Genetics	Streptomyces spinosus sp. nov. and Streptomyces shenzhenensis subsp. oryzicola subsp. nov. endophytic actinobacteria isolated from Jasmine rice and their genome mining for potential as antibiotic producers and plant growth promoters.	Kaewkla O, Sukpanoa S, Suriyachadkun C, Chamroensaksi N, Chumroenphat T, Franco CMM.	Antonie Van Leeuwenhoek	10.1007/s10482-022-01741-9	2022
Phylogeny	Effusibacillus dendaii sp. nov. isolated from farm soil.	Konishi T, Tamura T, Tobita T, Sakai S, Matsuda N, Kawasaki H.	Arch Microbiol	10.1007/s00203-021-02470-9	2021
Phylogeny	Streptomyces kebangsaanensis sp. nov., an endophytic actinomycete isolated from an ethnomedicinal plant, which produces phenazine-1-carboxylic acid.	Sarmin NIM, Tan GYA, Franco CMM, Edrada-Ebel R, Latip J, Zin NM.	Int J Syst Evol Microbiol	10.1099/ijs.0.047878-0	2013
Metabolism	Streptomyces monashensis sp. nov., a novel mangrove soil actinobacterium from East Malaysia with antioxidative potential.	Law JW, Ser HL, Ab Mutalib NS, Saokaew S, Duangjai A, Khan TM, Chan KG, Goh BH, Lee LH.	Sci Rep	10.1038/s41598-019-39592-6	2019
Phylogeny	Alicyclobacillus consociatus sp. nov., isolated from a human clinical specimen.	Glaeser SP, Falsen E, Martin K, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.048173-0	2013
Phylogeny	Alicyclobacillus pohliae sp. nov., a thermophilic, endospore-forming bacterium isolated from geothermal soil of the north-west slope of Mount Melbourne (Antarctica).	Imperio T, Viti C, Marri L	Int J Syst Evol Microbiol	10.1099/ijs.0.65092-0	2008

References

#16497	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22757
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#28428	IJSEM 221 2008 ( DOI 10.1099/ijs.0.65092-0 , PubMed 18175712 )
#32186	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28428
#36573	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121921	Collection of Institut Pasteur ; Curators of the CIP; CIP 109385
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Effusibacillus pohliae (DSM 22757, CIP 109385, NCIMB 14276)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000376225

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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