Name: Effusibacillus pohliae MP4
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 434

Type strain:

Species: Effusibacillus pohliae

Strain Designation: MP4

Culture col. no.: DSM 22757, CIP 109385, NCIMB 14276

Strain history: CIP <- 2006, L. Marri, Siena Univ., Siena, Italy, "Alicyclobacillus melbournensis": strain MP4

NCBI tax ID(s): 1120973 (strain), 232270 (species)

SI-ID 323943

CIP 109385, NCIMB 14276, DSM 22757

Other strains from Effusibacillus pohliae

E. pohliae SF006210(FSU),
E. pohliae SF002674(FSU),
E. pohliae SF002675(FSU),

Section

Effusibacillus pohliae MP4 is an aerobe, spore-forming, thermophilic bacterium that was isolated from geothermal soil .

Gram-positive
rod-shaped
spore-forming
aerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Alicyclobacillaceae*
	Genus	*Effusibacillus*
	Species	**Effusibacillus pohliae**
	Full Scientific Name (LPSN)	Effusibacillus pohliae (Imperio et al. 2008) Watanabe et al. 2014

Synonym

Alicyclobacillus pohliae

Information on morphological and physiological properties Morphology

[Ref.: #32186]	Gram stain	positive
[Ref.: #121921]	Gram stain	negative
[Ref.: #69480]	Gram stain	positive Confidence in %99.999

[Ref.: #32186]	Cell length	2 µm

[Ref.: #32186]	Cell width	0.5 µm

[Ref.: #32186]	Cell shape	rod-shaped
[Ref.: #121921]	Cell shape	rod-shaped

[Ref.: #121921]	Motility	no
[Ref.: #69480]	Motility	yes Confidence in %93.472

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16497]

Culture medium

ALICYCLOBACILLUS POHLIAE MEDIUM (DSMZ Medium 1209)

[Ref.: #16497]

Culture medium growth

[Ref.: #16497]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16497]

Culture medium composition

expand / minimize

[Ref.: #36573]

Culture medium

MEDIUM 685 - for Alicyclobacillus melbournensis

[Ref.: #36573]

Culture medium growth

[Ref.: #121921]

Culture medium

CIP Medium 685

[Ref.: #121921]

Culture medium growth

[Ref.: #121921]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #16497]	growth	55 °C
[Ref.: #32186]	growth	42-60 °C
[Ref.: #32186]	optimum	55 °C
[Ref.: #36573]	growth	55 °C

[Ref.: #16497]	Temperature range	thermophilic
[Ref.: #32186]	Temperature range	thermophilic
[Ref.: #36573]	Temperature range	thermophilic
[Ref.: #69480]	Temperature range	thermophilic Confidence in %99.633

	pH	Kind of pH		pH
[Ref.: #32186]			optimum	5.5
[Ref.: #32186]			growth	4.5-7.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #32186]	Oxygen tolerance	aerobe
[Ref.: #121921]	Oxygen tolerance	obligate aerobe

[Ref.: #32186]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.999

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #32186]	2-dehydro-D-gluconate ChEBI	+	carbon source
[Ref.: #32186]	5-dehydro-D-gluconate ChEBI	+	carbon source
[Ref.: #32186]	arabinose ChEBI	+	carbon source
[Ref.: #32186]	cellobiose ChEBI	+	carbon source
[Ref.: #32186]	fructose ChEBI	+	carbon source
[Ref.: #32186]	fucose ChEBI	+	carbon source
[Ref.: #32186]	galactose ChEBI	+	carbon source
[Ref.: #32186]	glucose ChEBI	+	carbon source
[Ref.: #32186]	glycogen ChEBI	+	carbon source
[Ref.: #32186]	lactose ChEBI	+	carbon source
[Ref.: #32186]	maltose ChEBI	+	carbon source
[Ref.: #32186]	mannose ChEBI	+	carbon source
[Ref.: #32186]	melibiose ChEBI	+	carbon source
[Ref.: #32186]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #121921]	nitrate ChEBI	+	reduction
[Ref.: #121921]	nitrite ChEBI	-	reduction
[Ref.: #32186]	rhamnose ChEBI	+	carbon source
[Ref.: #32186]	ribose ChEBI	+	carbon source
[Ref.: #32186]	sucrose ChEBI	+	carbon source
[Ref.: #32186]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #121921]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121921]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121921]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121921]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121921]	11830	-	-	+	+	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16497]	Sample type/isolated from	geothermal soil (in the immediate vicinity of a steam vent)
[Ref.: #16497]	Geographic location (country and/or sea, region)	northern Victoria Land, north-west slope of Mount Melbourne (74°20.994'S 164°39.792'E, 2498 m altitude)
[Ref.: #16497]	Continent	Australia and Oceania
[Ref.: #16497]	Geographic location	-74.3499°/164.6630°

[Ref.: #121921]	Sample type/isolated from	Environment, Geothermal soil
[Ref.: #121921]	Geographic location (country and/or sea, region)	Mount Melbourne, Victoria land
[Ref.: #121921]	Country	Antarctica
[Ref.: #121921]	Country ISO 3 Code	ATA
[Ref.: #121921]	Continent	Antarctica
[Ref.: #121921]	Isolation date	2002
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ564766 (>99% sequence identity) for Effusibacillus pohliae subclade from Microbeatlas

Aquatic Samples	155
Soil Samples	132
Animal Samples	1191
Plant Samples	31
Total Samples	1509

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16497]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121921]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16497]	Alicyclobacillus pohliae partial 16S rRNA gene, type strain CIP 109385T	AJ564766	1505	ENA	232270 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Effusibacillus pohliae DSM 22757	GCA_000376225	contig	GenBank	1120973 tax ID	*
[Ref.: #66792]	Alicyclobacillus pohliae DSM 22757	1120973.4	wgs	PATRIC	1120973 tax ID	*
[Ref.: #66792]	Effusibacillus pohliae DSM 22757	2518645625	draft	JGI IMG/M	1120973 tax ID	*

[Ref.: #16497]

GC-content

55.1 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Alicyclobacillus pohliae DSM 22757 PATRIC: 1120973.4
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Alicyclobacillus pohliae DSM 22757 PATRIC: 1120973.4
[Ref.: #69480]	motile	yes	82.198	no
[Ref.: #69480]	flagellated	no	57.588	no
[Ref.: #69480]	gram-positive	yes	88.105	yes
[Ref.: #69480]	anaerobic	no	97.012	yes
[Ref.: #69480]	aerobic	yes	89.538	yes
[Ref.: #69480]	halophile	no	95.022	no
[Ref.: #69480]	spore-forming	yes	96.483	yes
[Ref.: #69480]	glucose-util	yes	89.087	yes
[Ref.: #69480]	thermophile	yes	99.999	yes
[Ref.: #69480]	glucose-ferment	no	91.751	no

Availability in culture collections External links

[Ref.: #16497]

Culture collection no.

DSM 22757, CIP 109385, NCIMB 14276

[Ref.: #70113]

SI-ID 323943

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Alicyclobacillus consociatus sp. nov., isolated from a human clinical specimen.	Glaeser SP, Falsen E, Martin K, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.048173-0	2013	*
Phylogeny	Alicyclobacillus pohliae sp. nov., a thermophilic, endospore-forming bacterium isolated from geothermal soil of the north-west slope of Mount Melbourne (Antarctica).	Imperio T, Viti C, Marri L	Int J Syst Evol Microbiol	10.1099/ijs.0.65092-0	2008	*

References

#16497

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22757

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32186

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28428 (see below)

#36573 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70113

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID323943.1 )

#121921 Collection of Institut Pasteur ; Curators of the CIP; CIP 109385

#28428

IJSEM 221 2008 ( DOI 10.1099/ijs.0.65092-0 , PubMed 18175712 )

* These data were automatically processed and therefore are not curated

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Alicyclobacillus pohliae DSM 22757

Alicyclobacillus pohliae DSM 22757

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