Strain identifier

BacDive ID: 434

Type strain: Yes

Species: Effusibacillus pohliae

Strain Designation: MP4

Strain history: CIP <- 2006, L. Marri, Siena Univ., Siena, Italy, "Alicyclobacillus melbournensis": strain MP4

NCBI tax ID(s): 1120973 (strain), 232270 (species)

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General

@ref: 16497

BacDive-ID: 434

DSM-Number: 22757

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, thermophilic, Gram-positive, rod-shaped

description: Effusibacillus pohliae MP4 is an aerobe, spore-forming, thermophilic bacterium that was isolated from geothermal soil .

NCBI tax id

NCBI tax idMatching level
1120973strain
232270species

strain history

@refhistory
365732006, L. Marri, Siena Univ., Sienna, Italy, "Alicyclobacillus melbournensis": strain MP4
16497<- CIP <- L. Marri, Univ. Siena, Italy; MP4 (Alicyclobacillus melbournensis)
121921CIP <- 2006, L. Marri, Siena Univ., Siena, Italy, "Alicyclobacillus melbournensis": strain MP4

doi: 10.13145/bacdive434.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Alicyclobacillaceae
  • genus: Effusibacillus
  • species: Effusibacillus pohliae
  • full scientific name: Effusibacillus pohliae (Imperio et al. 2008) Watanabe et al. 2014

  • synonyms

    • @ref: 20215
    • synonym: Alicyclobacillus pohliae

@ref: 16497

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Alicyclobacillaceae

genus: Effusibacillus

species: Effusibacillus pohliae

full scientific name: Effusibacillus pohliae (Imperio et al. 2008) Watanabe et al. 2014

strain designation: MP4

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
32186positive2 µm0.5 µmrod-shaped
69480yes93.472
69480positive99.999
121921negativerod-shapedno

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16497ALICYCLOBACILLUS POHLIAE MEDIUM (DSMZ Medium 1209)yeshttps://mediadive.dsmz.de/medium/1209Name: ALICYCOBACILLUS POHLIAE MEDIUM (DSMZ Medium 1209) Composition: Agar 15.0 g/l Tryptone 5.0 g/l D-Glucose 0.5 g/l (NH4)2SO4 0.1 g/l FeSO4 x 7 H2O 0.07 g/l Distilled water
36573MEDIUM 685 - for Alicyclobacillus melbournensisyes
121921CIP Medium 685yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=685

culture temp

@refgrowthtypetemperaturerangeconfidence
16497positivegrowth55thermophilic
32186positivegrowth42-60thermophilic
32186positiveoptimum55thermophilic
36573positivegrowth55thermophilic
69480thermophilic99.633

culture pH

@refabilitytypepH
32186positivegrowth4.5-7.5
32186positiveoptimum5.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
32186aerobe
121921obligate aerobe

spore formation

@refspore formationconfidence
32186yes
69481yes100
69480yes99.999

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
32186168082-dehydro-D-gluconate+carbon source
32186581435-dehydro-D-gluconate+carbon source
3218622599arabinose+carbon source
3218617057cellobiose+carbon source
3218628757fructose+carbon source
3218633984fucose+carbon source
3218628260galactose+carbon source
3218617234glucose+carbon source
3218628087glycogen+carbon source
3218617716lactose+carbon source
3218617306maltose+carbon source
3218637684mannose+carbon source
3218628053melibiose+carbon source
32186506227N-acetylglucosamine+carbon source
3218626546rhamnose+carbon source
3218633942ribose+carbon source
3218617992sucrose+carbon source
3218618222xylose+carbon source
12192117632nitrate+reduction
12192116301nitrite-reduction

metabolite production

  • @ref: 121921
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
121921oxidase+
121921catalase+1.11.1.6
121921urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121921--++------++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcontinentlatitudelongitudecountryorigin.countryisolation date
16497geothermal soil (in the immediate vicinity of a steam vent)northern Victoria Land, north-west slope of Mount Melbourne (74°20.994'S 164°39.792'E, 2498 m altitude)Australia and Oceania-74.3499164.663
121921Environment, Geothermal soilMount Melbourne, Victoria landAntarcticaAntarcticaATA2002

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_56256.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1221;96_9387;97_11285;98_13877;99_56256&stattab=map
  • Last taxonomy: Effusibacillus pohliae subclade
  • 16S sequence: AJ564766
  • Sequence Identity:
  • Total samples: 1509
  • soil counts: 132
  • aquatic counts: 155
  • animal counts: 1191
  • plant counts: 31

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
164971Risk group (German classification)
1219211Risk group (French classification)

Sequence information

16S sequences

  • @ref: 16497
  • description: Alicyclobacillus pohliae partial 16S rRNA gene, type strain CIP 109385T
  • accession: AJ564766
  • length: 1505
  • database: ena
  • NCBI tax ID: 232270

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Effusibacillus pohliae DSM 22757GCA_000376225contigncbi1120973
66792Alicyclobacillus pohliae DSM 227571120973.4wgspatric1120973
66792Effusibacillus pohliae DSM 227572518645625draftimg1120973

GC content

  • @ref: 16497
  • GC-content: 55.1

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes82.198no
flagellatedno57.588no
gram-positiveyes88.105yes
anaerobicno97.012yes
aerobicyes89.538yes
halophileno95.022no
spore-formingyes96.483yes
glucose-utilyes89.087yes
thermophileyes99.999yes
glucose-fermentno91.751no

External links

@ref: 16497

culture collection no.: DSM 22757, CIP 109385, NCIMB 14276

straininfo link

  • @ref: 70113
  • straininfo: 323943

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18175712Alicyclobacillus pohliae sp. nov., a thermophilic, endospore-forming bacterium isolated from geothermal soil of the north-west slope of Mount Melbourne (Antarctica).Imperio T, Viti C, Marri LInt J Syst Evol Microbiol10.1099/ijs.0.65092-02008Antarctic Regions, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, Fatty Acids/analysis, Genes, rRNA, Gram-Positive Endospore-Forming Rods/*classification/genetics/*isolation & purification/physiology, *Hot Temperature, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Species SpecificityStress
Phylogeny23606481Alicyclobacillus consociatus sp. nov., isolated from a human clinical specimen.Glaeser SP, Falsen E, Martin K, Kampfer PInt J Syst Evol Microbiol10.1099/ijs.0.048173-02013Alicyclobacillus/*classification/genetics/isolation & purification, Base Composition, Blood/*microbiology, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Female, Humans, Middle Aged, Molecular Sequence Data, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Vitamin K 2/analogs & derivatives/analysisPathogenicity

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16497Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22757)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22757
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32186Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2842828776041
36573Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7120
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70113Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID323943.1StrainInfo: A central database for resolving microbial strain identifiers
121921Curators of the CIPCollection of Institut Pasteur (CIP 109385)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109385