Name: Dietzia natronolimnaea 15LN1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 4252

Type strain:

Species: Dietzia natronolimnaea

Strain Designation: 15LN1

Culture col. no.: DSM 44860, CBS 107.95, JCM 11417, CCUG 54951, NBRC 105040

Strain history: W. D. Grant 15LN1.

NCBI tax ID(s): 161920 (species)

SI-ID 97722

CBS 107.95, JCM 11417, DSM 44860, CCUG 54951

Special Collections

Other strains from Dietzia natronolimnaea

Section

Dietzia natronolimnaea 15LN1 is a microaerophile bacterium that was isolated from soda lake.

microaerophile
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Dietziaceae*
	Genus	*Dietzia*
	Species	**Dietzia natronolimnaea**
	Full Scientific Name (LPSN)	Dietzia natronolimnaea corrig. Duckworth et al. 1999

Synonym

Dietzia natronolimnaios

Information on morphological and physiological properties Morphology

Multimedia content

[Ref.: #12147]

Caption:

Medium 535 28°C

[Ref.: #12147]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #12147]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #12147]

Culture medium growth

[Ref.: #12147]

Culture medium link

Medium recipe at MediaDive

[Ref.: #12147]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #12147]	growth	28 °C
[Ref.: #60411]	growth	37 °C
[Ref.: #67770]	growth	28 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #60411]

Oxygen tolerance

microaerophile

[Ref.: #67770]

Observation

quinones: MK-8(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	nitrate ChEBI	+	reduction	* from API Coryne
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API Coryne
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API Coryne
[Ref.: #68379]	catalase	+	1.11.1.6	* from API Coryne
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #60411]	1718	+	-	+	+	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #60411]	2087	-	+	-	+	-	+	-	+	+	-	-	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #12147]	Sample type/isolated from	soda lake
[Ref.: #12147]	Geographic location (country and/or sea, region)	Lake Oloidien (0° 49' S, 36° 13' E)
[Ref.: #12147]	Country	Kenya
[Ref.: #12147]	Country ISO 3 Code	KEN
[Ref.: #12147]	Continent	Africa
[Ref.: #12147]	Geographic location	-0.8167°/36.2167°

[Ref.: #60411]	Sample type/isolated from	Soda Lake
[Ref.: #60411]	Geographic location (country and/or sea, region)	East Africa
[Ref.: #60411]	Continent	Africa

[Ref.: #67770]	Sample type/isolated from	Littoral sediments of Lake Oloidien (Little Lake Naivasha)
[Ref.: #67770]	Country	Kenya
[Ref.: #67770]	Country ISO 3 Code	KEN
[Ref.: #67770]	Continent	Africa
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Condition	#Alkaline	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #12147]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Dietzia natronolimnaea 16S rRNA gene, strain 15LN1; CBS 107.95	X92157	1456	GenBank ENA	161920 tax ID	*
[Ref.: #20218]	Dietzia natronolimnaea strain DSM 44860 16S ribosomal RNA gene, partial sequence	FJ468329	1439	GenBank ENA	161920 tax ID	*

[Ref.: #67770]

GC-content

66.1 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #12147]

Culture collection no.

DSM 44860, CBS 107.95, JCM 11417, CCUG 54951, NBRC 105040

[Ref.: #73749]

SI-ID 97722

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Dietzia schimae sp. nov. and Dietzia cercidiphylli sp. nov., from surface-sterilized plant tissues.	Li J, Zhao GZ, Zhang YQ, Klenk HP, Pukall R, Qin S, Xu LH, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.2008/000919-0	2008	*
Phylogeny	Dietzia natronolimnaios sp. nov., a new member of the genus Dietzia isolated from an east African soda lake.	Duckworth AW, Grant S, Grant WD, Jones BE, Meijer D	Extremophiles	10.1007/s007920050079	1998	*

References

#12147

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44860

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#60411 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 54951
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#73749

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID97722.1 )

* These data were automatically processed and therefore are not curated

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