Strain identifier

BacDive ID: 4252

Type strain: Yes

Species: Dietzia natronolimnaea

Strain Designation: 15LN1

Strain history: W. D. Grant 15LN1.

NCBI tax ID(s): 161920 (species)

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General

@ref: 12147

BacDive-ID: 4252

DSM-Number: 44860

keywords: 16S sequence, Bacteria, microaerophile, mesophilic

description: Dietzia natronolimnaea 15LN1 is a microaerophile, mesophilic bacterium that was isolated from soda lake.

NCBI tax id

  • NCBI tax id: 161920
  • Matching level: species

strain history

@refhistory
12147<- JCM <- W. D. Grant; 15LN1
67770W. D. Grant 15LN1.

doi: 10.13145/bacdive4252.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Dietziaceae
  • genus: Dietzia
  • species: Dietzia natronolimnaea
  • full scientific name: Dietzia natronolimnaea corrig. Duckworth et al. 1999

  • synonyms

    • @ref: 20215
    • synonym: Dietzia natronolimnaios

@ref: 12147

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Dietziaceae

genus: Dietzia

species: Dietzia natronolimnaea

full scientific name: Dietzia natronolimnaea Duckworth et al. 1999

strain designation: 15LN1

type strain: yes

Morphology

multimedia

  • @ref: 12147
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44860.jpg
  • caption: Medium 535 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

  • @ref: 12147
  • name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/535
  • composition: Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
12147positivegrowth28mesophilic
60411positivegrowth37mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 60411
  • oxygen tolerance: microaerophile

observation

  • @ref: 67770
  • observation: quinones: MK-8(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837917632nitrate+reduction
683794853esculin+hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
68379catalase+1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlGLURIBXYLMANMALLACSACGLYGCAT
60411+-++--+-+-----------+

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
60411-+-+-+-++--+---+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
12147soda lakeLake Oloidien (0° 49' S, 36° 13' E)KenyaKENAfrica-0.816736.2167
60411Soda LakeEast AfricaAfrica
67770Littoral sediments of Lake Oloidien (Little Lake Naivasha)KenyaKENAfrica

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Condition#Alkaline

Safety information

risk assessment

  • @ref: 12147
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Dietzia natronolimnaea 16S rRNA gene, strain 15LN1; CBS 107.95X921571456ena161920
20218Dietzia natronolimnaea strain DSM 44860 16S ribosomal RNA gene, partial sequenceFJ4683291439ena161920

GC content

  • @ref: 67770
  • GC-content: 66.1
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 12147

culture collection no.: DSM 44860, CBS 107.95, JCM 11417, CCUG 54951, NBRC 105040

straininfo link

  • @ref: 73749
  • straininfo: 97722

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny9783184Dietzia natronolimnaios sp. nov., a new member of the genus Dietzia isolated from an east African soda lake.Duckworth AW, Grant S, Grant WD, Jones BE, Meijer DExtremophiles10.1007/s0079200500791998Bacteria, Aerobic/classification/genetics/*isolation & purification, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Environment, Fresh Water/*microbiology, Hydrogen-Ion Concentration, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sodium ChlorideEnzymology
Phylogeny18984691Dietzia schimae sp. nov. and Dietzia cercidiphylli sp. nov., from surface-sterilized plant tissues.Li J, Zhao GZ, Zhang YQ, Klenk HP, Pukall R, Qin S, Xu LH, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.2008/000919-02008Actinomycetales/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, Plant Roots/microbiology, Plant Stems/microbiology, Plants/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Sterilization/methods, Theaceae/*microbiologyGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
12147Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44860)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44860
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
60411Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 54951)https://www.ccug.se/strain?id=54951
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
73749Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID97722.1StrainInfo: A central database for resolving microbial strain identifiers