Name: Desulfurococcus amylolyticus 1221n
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 4200

Type strain:

Species: Desulfurococcus amylolyticus

Strain Designation: 1221n

Culture col. no.: DSM 18924, JCM 16383, VKM B-2413

Strain history: DSM 18924 <-- I. V. Kublanov 1221n.

NCBI tax ID(s): 94694 (species)

SI-ID 402929

DSM 18924

Other strains from Desulfurococcus amylolyticus

D. amylolyticus DSM 3822, JCM 9188 (type strain)
D. amylolyticus Z-1312, DSM 16532, JCM 13383, VKM ...

Section

Desulfurococcus amylolyticus 1221n is an anaerobe, chemoorganoheterotroph, hyperthermophilic archaeon that was isolated from mixed water and sediment.

coccus-shaped
anaerobe
chemoorganoheterotroph
hyperthermophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Thermoproteota*
	Class	*Thermoprotei*
	Order	*Desulfurococcales*
	Family	*Desulfurococcaceae*
	Genus	*Desulfurococcus*
	Species	**Desulfurococcus amylolyticus**
	Full Scientific Name (LPSN)	Desulfurococcus amylolyticus Bonch-Osmolovskaya et al. 2001

	Synonym	Desulfurococcus fermentans
	Synonym	Desulfurococcus kamchatkensis

Information on morphological and physiological properties Morphology

[Ref.: #23226]	Cell length	0.6-1.0 µm

[Ref.: #23226]	Cell shape	coccus-shaped

[Ref.: #23226]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7778]

Culture medium

DESULFUROCOCCUS MEDIUM (DSMZ Medium 395)

[Ref.: #7778]

Culture medium growth

[Ref.: #7778]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7778]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #7778]	growth	82 °C
[Ref.: #23226]	growth	65-87 °C
[Ref.: #23226]	optimum	85 °C
[Ref.: #67770]	growth	82 °C

[Ref.: #7778]	Temperature range	hyperthermophilic
[Ref.: #23226]	Temperature range	hyperthermophilic
[Ref.: #67770]	Temperature range	hyperthermophilic

	pH	Kind of pH		pH
[Ref.: #23226]			optimum	6.5
[Ref.: #23226]			growth	5.5-7.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #7778]	Oxygen tolerance	anaerobe
[Ref.: #23226]	Oxygen tolerance	obligate anaerobe

[Ref.: #23226]

Nutrition type

chemoorganoheterotroph

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23226]	acetate ChEBI	-	carbon source
[Ref.: #23226]	arabinose ChEBI	-	carbon source
[Ref.: #23226]	carboxymethylcellulose ChEBI	-	carbon source
[Ref.: #23226]	casamino acids	-	carbon source
[Ref.: #23226]	casein	-	carbon source
[Ref.: #23226]	casein hydrolysate	-	carbon source
[Ref.: #23226]	cellobiose ChEBI	-	carbon source
[Ref.: #23226]	cellulose ChEBI	-	carbon source
[Ref.: #23226]	chitin ChEBI	-	carbon source
[Ref.: #23226]	dextran ChEBI	+	energy source
[Ref.: #23226]	dextran ChEBI	+	fermentation
[Ref.: #23226]	dextrin ChEBI	+	energy source
[Ref.: #23226]	dextrin ChEBI	+	fermentation
[Ref.: #23226]	elemental sulfur ChEBI	+	electron acceptor
[Ref.: #23226]	ethanol ChEBI	-	carbon source
[Ref.: #23226]	fructose ChEBI	-	carbon source
[Ref.: #23226]	fumarate ChEBI	-	carbon source
[Ref.: #23226]	galactose ChEBI	-	carbon source
[Ref.: #23226]	gelatin ChEBI	+	energy source
[Ref.: #23226]	gelatin ChEBI	+	fermentation
[Ref.: #23226]	glucose ChEBI	+	energy source
[Ref.: #23226]	glucose ChEBI	+	fermentation
[Ref.: #23226]	lactose ChEBI	-	carbon source
[Ref.: #23226]	maltose ChEBI	-	carbon source
[Ref.: #23226]	methanol ChEBI	-	carbon source
[Ref.: #23226]	nitrate ChEBI	-	growth
[Ref.: #23226]	pectin ChEBI	-	carbon source
[Ref.: #23226]	peptone	+	energy source
[Ref.: #23226]	peptone	+	fermentation
[Ref.: #23226]	pyruvate ChEBI	-	carbon source
[Ref.: #23226]	starch ChEBI	-	carbon source
[Ref.: #23226]	succinate ChEBI	-	carbon source
[Ref.: #23226]	sucrose ChEBI	+	energy source
[Ref.: #23226]	sucrose ChEBI	+	fermentation
[Ref.: #23226]	sulfite ChEBI	-	growth
[Ref.: #23226]	thiosulfate ChEBI	-	growth
[Ref.: #23226]	tryptone	+	energy source
[Ref.: #23226]	tryptone	+	fermentation
[Ref.: #23226]	xylan ChEBI	-	carbon source
[Ref.: #23226]	yeast extract	+	energy source
[Ref.: #23226]	yeast extract	+	fermentation
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7778]	Sample type/isolated from	mixed water and sediment
[Ref.: #7778]	Geographic location (country and/or sea, region)	Kamchatka, Uzon Caldera, Izvilistyi hot spring (54°50'0.6'' N, 160°00'57.1'' E)
[Ref.: #7778]	Country	Russia
[Ref.: #7778]	Country ISO 3 Code	RUS
[Ref.: #7778]	Continent	Asia
[Ref.: #7778]	Geographic location	54.8335°/160.0160°

[Ref.: #23226]	Sample type/isolated from	Izvilistyi spring of Central Field

[Ref.: #67770]	Sample type/isolated from	Hot spring of Uzon
[Ref.: #67770]	Geographic location (country and/or sea, region)	Caldera, Kamchatka Peninsula
[Ref.: #67770]	Country	Russia
[Ref.: #67770]	Country ISO 3 Code	RUS
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Spring
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU167539 (>99% sequence identity) for Desulfurococcus amylolyticus subclade from Microbeatlas

Aquatic Samples	25
Soil Samples
Animal Samples	2
Plant Samples
Total Samples	27

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7778]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7778]	Desulfurococcus kamchatkensis strain 1221n 16S ribosomal RNA gene, complete sequence	EU167539	1496	ENA	490899 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Desulfurococcus amylolyticus 1221n	GCA_000020905	complete	GenBank	490899 tax ID	*

[Ref.: #7778]	GC-content	44.4 mol%
[Ref.: #23226]	GC-content	44.4 mol%	Thermal denaturation, fluorometry
[Ref.: #67770]	GC-content	44.4 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Desulfurococcus amylolyticus 1221n 1221n NCBI: GCA_000020905.1
[Ref.: #69480]	motile	no	64.398	yes
[Ref.: #69480]	flagellated	no	90.96	no
[Ref.: #69480]	gram-positive	no	94.65	no
[Ref.: #69480]	anaerobic	yes	99.064	yes
[Ref.: #69480]	aerobic	no	96.526	yes
[Ref.: #69480]	halophile	no	78.144	no
[Ref.: #69480]	spore-forming	no	98.086	no
[Ref.: #69480]	thermophile	yes	100	no
[Ref.: #69480]	glucose-util	yes	77.73	no
[Ref.: #69480]	glucose-ferment	no	58.149	yes

Availability in culture collections External links

[Ref.: #7778]

Culture collection no.

DSM 18924, JCM 16383, VKM B-2413

[Ref.: #73700]

SI-ID 402929

Literature:

	Title	Authors	Journal	DOI	Year
Phylogeny	Desulfurococcus kamchatkensis sp. nov., a novel hyperthermophilic protein-degrading archaeon isolated from a Kamchatka hot spring.	Kublanov IV, Bidjieva SKh, Mardanov AV, Bonch-Osmolovskaya EA	Int J Syst Evol Microbiol	10.1099/ijs.0.006726-0	2009	*
	Identified Hybrid tRNA Structure Genes in Archaeal Genome.	Mandal UR, Das SS, Chattopadhyay B, Sahoo S	Iran J Biotechnol	10.29252/ijb.2254	2019	*

References

#7778

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18924

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23226

I. V. Kublanov, S. Kh. Bidjieva, A. V. Mardanov, E. A. Bonch-Osmolovskaya: Desulfurococcus kamchatkensis sp. nov., a novel hyperthermophilic protein-degrading archaeon isolated from a Kamchatka hot spring. IJSEM 59: 1743 - 1747 2009 ( DOI 10.1099/ijs.0.006726-0 , PubMed 19542129 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#73700

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402929.1 )

* These data were automatically processed and therefore are not curated

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Desulfurococcus amylolyticus 1221n 1221n

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