Name: Alicyclobacillus acidiphilus TA-67
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 414

Type strain:

Species: Alicyclobacillus acidiphilus

Strain Designation: TA-67

Culture col. no.: DSM 14558, IAM 14935, NRIC 0496, JCM 21417, CIP 107723, NBRC 100859, NRIC 6496

Strain history: CIP <- 2002, IAM <- H Matsubara: strain TA-67

NCBI tax ID(s): 1255277 (strain), 182455 (species)

SI-ID 100493

DSM 14558, IAM 14935, KCTC 3799, NBRC 100859, CBMAI 247, JCM 21417, CIP 107723

Section

Alicyclobacillus acidiphilus TA-67 is an aerobe, spore-forming, thermophilic bacterium that forms circular colonies and was isolated from acidic beverage, that was off-flavored.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
aerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Alicyclobacillaceae*
	Genus	*Alicyclobacillus*
	Species	**Alicyclobacillus acidiphilus**
	Full Scientific Name (LPSN)	Alicyclobacillus acidiphilus Matsubara et al. 2002

Information on morphological and physiological properties Morphology

[Ref.: #23285]	Gram stain	positive
[Ref.: #116003]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #23285]	Cell length	4.8-6.3 µm

[Ref.: #23285]	Cell width	0.9-1.1 µm

[Ref.: #23285]	Cell shape	rod-shaped
[Ref.: #116003]	Cell shape	rod-shaped

[Ref.: #23285]	Motility	yes
[Ref.: #116003]	Motility	no
[Ref.: #69480]	Motility	yes Confidence in %92.82

[Ref.: #23285]	Colony size	1.1-3.8 mm
[Ref.: #23285]	Colony color	creamy white
[Ref.: #23285]	Colony shape	circular
[Ref.: #23285]	Incubation period	3 days
[Ref.: #23285]	Cultivation medium used	BAM agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5384]

Culture medium

ALICYCLOBACILLUS MEDIUM (DSMZ Medium 402)

[Ref.: #5384]

Culture medium growth

[Ref.: #5384]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5384]

Culture medium composition

expand / minimize

[Ref.: #23285]

Culture medium

BAM agar

[Ref.: #23285]

Culture medium growth

[Ref.: #38174]

Culture medium

MEDIUM 413 - for Alicyclobacillus hesperidum

[Ref.: #38174]

Culture medium growth

[Ref.: #38174]

Culture medium composition

expand / minimize

[Ref.: #116003]

Culture medium

CIP Medium 72

[Ref.: #116003]

Culture medium growth

[Ref.: #116003]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #116003]

Culture medium

CIP Medium 413

[Ref.: #116003]

Culture medium growth

[Ref.: #116003]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5384]		growth	45 °C
[Ref.: #23285]		growth	20-55 °C
[Ref.: #23285]		optimum	50 °C
[Ref.: #38174]		growth	45 °C
[Ref.: #67770]		growth	50 °C
[Ref.: #116003]		growth	37-45 °C
[Ref.: #116003]	no	growth	10 °C
[Ref.: #116003]	no	growth	22 °C

[Ref.: #5384]	Temperature range	thermophilic
[Ref.: #23285]	Temperature range	thermophilic
[Ref.: #38174]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic
[Ref.: #116003]	Temperature range	psychrophilic

	Kind of pH		pH
[Ref.: #23285]		optimum	3.0
[Ref.: #23285]		growth	2.5-5.5
[Ref.: #116003]	no	growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23285]	Oxygen tolerance	aerobe
[Ref.: #116003]	Oxygen tolerance	obligate aerobe

[Ref.: #23285]	Spore description	ellipsoidal to oval and terminal to subterminal, and sporangia were swollen
[Ref.: #23285]	Type of spore	spore
[Ref.: #23285]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %99
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.447

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23285]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23285]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23285]	amygdalin ChEBI	-	builds acid from
[Ref.: #23285]	arbutin ChEBI	+	builds acid from
[Ref.: #23285]	cellobiose ChEBI	+	builds acid from
[Ref.: #23285]	D-arabinose ChEBI	+	builds acid from
[Ref.: #23285]	D-arabitol ChEBI	-	builds acid from
[Ref.: #23285]	D-fructose ChEBI	+	builds acid from
[Ref.: #23285]	D-fucose ChEBI	-	builds acid from
[Ref.: #23285]	D-glucose ChEBI	+	builds acid from
[Ref.: #23285]	D-lyxose ChEBI	-	builds acid from
[Ref.: #23285]	D-mannose ChEBI	+	builds acid from
[Ref.: #23285]	D-tagatose ChEBI	-	builds acid from
[Ref.: #23285]	D-xylose ChEBI	+	builds acid from
[Ref.: #23285]	erythritol ChEBI	-	builds acid from
[Ref.: #23285]	esculin ChEBI	+	builds acid from
[Ref.: #116003]	esculin ChEBI	+	hydrolysis
[Ref.: #23285]	galactitol ChEBI	-	builds acid from
[Ref.: #23285]	galactose ChEBI	+	builds acid from
[Ref.: #23285]	gelatin ChEBI	-	hydrolysis
[Ref.: #23285]	gentiobiose ChEBI	+	builds acid from
[Ref.: #23285]	gluconate ChEBI	-	builds acid from
[Ref.: #23285]	glucose ChEBI	-	builds gas from
[Ref.: #23285]	glycerol ChEBI	-	builds acid from
[Ref.: #23285]	glycogen ChEBI	-	builds acid from
[Ref.: #116003]	hippurate ChEBI	-	hydrolysis
[Ref.: #23285]	inulin ChEBI	-	builds acid from
[Ref.: #23285]	L-arabinose ChEBI	+	builds acid from
[Ref.: #23285]	L-arabitol ChEBI	-	builds acid from
[Ref.: #23285]	L-fucose ChEBI	-	builds acid from
[Ref.: #23285]	L-sorbose ChEBI	+	builds acid from
[Ref.: #23285]	L-xylose ChEBI	-	builds acid from
[Ref.: #23285]	lactose ChEBI	+	builds acid from
[Ref.: #23285]	maltose ChEBI	+	builds acid from
[Ref.: #23285]	mannitol ChEBI	-	builds acid from
[Ref.: #23285]	melezitose ChEBI	+	builds acid from
[Ref.: #23285]	melibiose ChEBI	-	builds acid from
[Ref.: #23285]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from
[Ref.: #23285]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #23285]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #23285]	myo-inositol ChEBI	-	builds acid from
[Ref.: #23285]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #23285]	nitrate ChEBI	-	reduction
[Ref.: #116003]	nitrate ChEBI	-	reduction
[Ref.: #116003]	nitrite ChEBI	-	reduction
[Ref.: #23285]	phenylalanine ChEBI	-	degradation
[Ref.: #23285]	raffinose ChEBI	+	builds acid from
[Ref.: #23285]	rhamnose ChEBI	-	builds acid from
[Ref.: #23285]	ribitol ChEBI	-	builds acid from
[Ref.: #23285]	ribose ChEBI	+	builds acid from
[Ref.: #23285]	salicin ChEBI	+	builds acid from
[Ref.: #23285]	sorbitol ChEBI	+	builds acid from
[Ref.: #23285]	starch ChEBI	-	builds acid from
[Ref.: #23285]	starch ChEBI	-	hydrolysis
[Ref.: #23285]	sucrose ChEBI	+	builds acid from
[Ref.: #23285]	trehalose ChEBI	+	builds acid from
[Ref.: #23285]	turanose ChEBI	+	builds acid from
[Ref.: #23285]	tyrosine ChEBI	-	degradation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #23285]	acetoin ChEBI	yes
[Ref.: #23285]	indole ChEBI	no
[Ref.: #116003]	indole ChEBI	no

	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #23285]	acetoin ChEBI	+/-
[Ref.: #116003]	acetoin ChEBI	-
[Ref.: #116003]	glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #116003]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #116003]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #116003]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #116003]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #116003]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #116003]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #116003]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #116003]	oxidase	-
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #116003]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116003]	10600	-	-	+	+	-	+	-	-	+	-	-	-	+	-	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5384]	Sample type/isolated from	acidic beverage, that was off-flavored
[Ref.: #5384]	Country	Japan
[Ref.: #5384]	Country ISO 3 Code	JPN
[Ref.: #5384]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Off-flavoured, acidic beverage

[Ref.: #116003]	Sample type/isolated from	Acidic beverage
[Ref.: #116003]	Country	Japan
[Ref.: #116003]	Country ISO 3 Code	JPN
[Ref.: #116003]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Beverage
#Condition	#Acidic	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB076660 (>99% sequence identity) for Alicyclobacillus acidiphilus subclade from Microbeatlas

Aquatic Samples	68
Soil Samples	472
Animal Samples	533
Plant Samples	272
Total Samples	1345

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5384]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116003]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5384]	Alicyclobacillus acidiphilus gene for 16S rRNA, partial sequence	AB076660	1542	ENA	182455 tax ID
[Ref.: #20218]	Alicyclobacillus acidiphilus strain DSM 14558 16S-23S ribosomal RNA intergenic spacer and 23S ribosomal RNA gene, partial sequence	GU208679	222	ENA	182455 tax ID	*
[Ref.: #20218]	Alicyclobacillus acidiphilus 16S ribosomal RNA gene, partial sequence	DQ279110	884	ENA	182455 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Alicyclobacillus acidiphilus NBRC 100859	GCA_001544355	contig	GenBank	1255277 tax ID
[Ref.: #66792]	Alicyclobacillus acidiphilus NBRC 100859	1255277.3	wgs	PATRIC	1255277 tax ID	*
[Ref.: #66792]	Alicyclobacillus acidiphilus NBRC 100859	2731957568	draft	JGI IMG/M	1255277 tax ID	*

[Ref.: #5384]	GC-content	54.1 mol%
[Ref.: #23285]	GC-content	54.1 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Alicyclobacillus acidiphilus NBRC 100859 PATRIC: 1255277.3
[Ref.: #69481]	spore-forming	yes	99	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Alicyclobacillus acidiphilus NBRC 100859 PATRIC: 1255277.3
[Ref.: #69480]	motile	yes	84.866	yes
[Ref.: #69480]	gram-positive	yes	88.325	yes
[Ref.: #69480]	anaerobic	no	98.937	no
[Ref.: #69480]	aerobic	yes	93.036	no
[Ref.: #69480]	halophile	no	90.937	no
[Ref.: #69480]	spore-forming	yes	94.523	yes
[Ref.: #69480]	thermophile	yes	96.185	yes
[Ref.: #69480]	glucose-util	yes	87.455	no
[Ref.: #69480]	flagellated	no	69.411	no
[Ref.: #69480]	glucose-ferment	no	87.878	no

Availability in culture collections External links

[Ref.: #5384]

Culture collection no.

DSM 14558, IAM 14935, NRIC 0496, JCM 21417, CIP 107723, NBRC 100859, NRIC 6496

[Ref.: #70094]

SI-ID 100493

Literature:

Topic	Title	Authors	Journal	DOI	Year
Biotechnology	Kinetics models for the inactivation of Alicyclobacillus acidiphilus DSM14558(T) and Alicyclobacillus acidoterrestris DSM 3922(T) in apple juice by ultrasound.	Wang J, Hu X, Wang Z	Int J Food Microbiol	10.1016/j.ijfoodmicro.2010.02.029	2010	*
Phylogeny	Alicyclobacillus acidiphilus sp. nov., a novel thermo-acidophilic, omega-alicyclic fatty acid-containing bacterium isolated from acidic beverages.	Matsubara H, Goto K, Matsumura T, Mochida K, Iwaki M, Niwa M, Yamasato K	Int J Syst Evol Microbiol	10.1099/00207713-52-5-1681	2002	*

References

#5384

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14558

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23285

Hiroshige Matsubara, Keiichi Goto, Terumi Matsumura, Kaoru Mochida, Masaharu Iwaki, Motohiro Niwa, Kazuhide Yamasato: Alicyclobacillus acidiphilus sp. nov., a novel thermo-acidophilic, omega-alicyclic fatty acid-containing bacterium isolated from acidic beverages.. IJSEM 52: 1681 - 1685 2002 ( DOI 10.1099/00207713-52-5-1681 , PubMed 12361274 )

#38174 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70094

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID100493.1 )

#116003 Collection of Institut Pasteur ; Curators of the CIP; CIP 107723
* These data were automatically processed and therefore are not curated

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Alicyclobacillus acidiphilus NBRC 100859

Alicyclobacillus acidiphilus NBRC 100859

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