Strain identifier

BacDive ID: 414

Type strain: Yes

Species: Alicyclobacillus acidiphilus

Strain Designation: TA-67

Strain history: CIP <- 2002, IAM <- H Matsubara: strain TA-67

NCBI tax ID(s): 1255277 (strain), 182455 (species)

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General

@ref: 5384

BacDive-ID: 414

DSM-Number: 14558

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, thermophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Alicyclobacillus acidiphilus TA-67 is an aerobe, spore-forming, thermophilic bacterium that forms circular colonies and was isolated from acidic beverage, that was off-flavored.

NCBI tax id

NCBI tax idMatching level
182455species
1255277strain

strain history

@refhistory
5384<- H. Matsubara; TA-67 (Alicyclobacillus acidophilus)
67770IAM 14935 <-- H. Matsubara TA-67.
116003CIP <- 2002, IAM <- H Matsubara: strain TA-67

doi: 10.13145/bacdive414.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Alicyclobacillaceae
  • genus: Alicyclobacillus
  • species: Alicyclobacillus acidiphilus
  • full scientific name: Alicyclobacillus acidiphilus Matsubara et al. 2002

@ref: 5384

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Alicyclobacillaceae

genus: Alicyclobacillus

species: Alicyclobacillus acidiphilus

full scientific name: Alicyclobacillus acidiphilus Matsubara et al. 2002

strain designation: TA-67

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
23285positive4.8-6.3 µm0.9-1.1 µmrod-shapedyes
69480yes92.82
69480positive100
116003positiverod-shapedno

colony morphology

@refcolony sizecolony colorcolony shapeincubation periodmedium used
232851.1-3.8 mmcreamy whitecircular3 daysBAM agar
116003

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5384ALICYCLOBACILLUS MEDIUM (DSMZ Medium 402)yeshttps://mediadive.dsmz.de/medium/402Name: ALICYCLOBACILLUS MEDIUM (DSMZ Medium 402) Composition: Agar 15.0 g/l Glucose 5.0 g/l KH2PO4 3.0 g/l Yeast extract 2.0 g/l MgSO4 x 7 H2O 0.5 g/l CaCl2 x 2 H2O 0.25 g/l (NH4)2SO4 0.2 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water
23285BAM agaryes
38174MEDIUM 413 - for Alicyclobacillus hesperidumyesDistilled water make up to (500.000 ml);Magnesium sulphate heptahydrate (0.500 g);Calcium chloride dihydrate (0.250 g);Glucose (5.000 g);Yeast extract (2.000 g);Ammonium sulphate (0.200 g);Potassium di-hydrogen phosphate (3.000 g);Solution SL-6 - M00545(1
116003CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
116003CIP Medium 413yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=413

culture temp

@refgrowthtypetemperaturerange
5384positivegrowth45thermophilic
23285positivegrowth20-55
23285positiveoptimum50thermophilic
38174positivegrowth45thermophilic
67770positivegrowth50thermophilic
116003positivegrowth37-45
116003nogrowth10psychrophilic
116003nogrowth22psychrophilic

culture pH

@refabilitytypepHPH range
23285positivegrowth2.5-5.5acidophile
23285positiveoptimum3.0
116003nogrowth6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23285aerobe
116003obligate aerobe

spore formation

@refspore descriptiontype of sporespore formationconfidence
23285ellipsoidal to oval and terminal to subterminal, and sporangia were swollensporeyes
69481yes99
69480yes99.447

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
23285168082-dehydro-D-gluconate-builds acid from
23285174265-dehydro-D-gluconate-builds acid from
2328515963ribitol-builds acid from
2328527613amygdalin-builds acid from
2328518333D-arabitol-builds acid from
2328528847D-fucose-builds acid from
2328562318D-lyxose-builds acid from
2328516443D-tagatose-builds acid from
2328516813galactitol-builds acid from
2328517113erythritol-builds acid from
2328524265gluconate-builds acid from
2328517754glycerol-builds acid from
2328528087glycogen-builds acid from
2328517268myo-inositol-builds acid from
2328515443inulin-builds acid from
2328518403L-arabitol-builds acid from
2328518287L-fucose-builds acid from
2328565328L-xylose-builds acid from
2328529864mannitol-builds acid from
2328528053melibiose-builds acid from
2328543943methyl alpha-D-mannoside-builds acid from
2328574863methyl beta-D-xylopyranoside-builds acid from
23285506227N-acetylglucosamine-builds acid from
2328526546rhamnose-builds acid from
2328528017starch-builds acid from
2328517234glucose-builds gas from
2328528044phenylalanine-degradation
2328518186tyrosine-degradation
232855291gelatin-hydrolysis
2328528017starch-hydrolysis
2328517632nitrate-reduction
2328518305arbutin+builds acid from
2328517057cellobiose+builds acid from
2328517108D-arabinose+builds acid from
2328515824D-fructose+builds acid from
2328517634D-glucose+builds acid from
2328516024D-mannose+builds acid from
2328516634raffinose+builds acid from
2328532528turanose+builds acid from
2328565327D-xylose+builds acid from
232854853esculin+builds acid from
2328528260galactose+builds acid from
2328528066gentiobiose+builds acid from
2328530849L-arabinose+builds acid from
2328517266L-sorbose+builds acid from
2328517716lactose+builds acid from
2328517306maltose+builds acid from
232856731melezitose+builds acid from
23285320061methyl alpha-D-glucopyranoside+builds acid from
2328533942ribose+builds acid from
2328517814salicin+builds acid from
2328530911sorbitol+builds acid from
2328517992sucrose+builds acid from
2328527082trehalose+builds acid from
1160034853esculin+hydrolysis
116003606565hippurate-hydrolysis
11600317632nitrate-reduction
11600316301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
2328535581indoleno
2328515688acetoinyes
11600335581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
2328515688acetoin+/-
11600315688acetoin-
11600317234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
116003oxidase-
116003beta-galactosidase-3.2.1.23
116003alcohol dehydrogenase-1.1.1.1
116003gelatinase-
116003catalase+1.11.1.6
116003gamma-glutamyltransferase+2.3.2.2
116003lysine decarboxylase-4.1.1.18
116003ornithine decarboxylase-4.1.1.17
116003urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116003--++-+--+---+--++---

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
5384acidic beverage, that was off-flavoredJapanJPNAsia
67770Off-flavoured, acidic beverage
116003Acidic beverageJapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Beverage
#Condition#Acidic

taxonmaps

  • @ref: 69479
  • File name: preview.99_36484.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_313;96_2480;97_21437;98_26884;99_36484&stattab=map
  • Last taxonomy: Alicyclobacillus acidiphilus subclade
  • 16S sequence: AB076660
  • Sequence Identity:
  • Total samples: 1345
  • soil counts: 472
  • aquatic counts: 68
  • animal counts: 533
  • plant counts: 272

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
53841Risk group (German classification)
1160031Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Alicyclobacillus acidiphilus 16S ribosomal RNA gene, partial sequenceDQ279110884ena182455
20218Alicyclobacillus acidiphilus strain DSM 14558 16S-23S ribosomal RNA intergenic spacer and 23S ribosomal RNA gene, partial sequenceGU208679222ena182455
5384Alicyclobacillus acidiphilus gene for 16S rRNA, partial sequenceAB0766601542ena182455

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Alicyclobacillus acidiphilus NBRC 1008591255277.3wgspatric1255277
66792Alicyclobacillus acidiphilus NBRC 1008592731957568draftimg1255277
67770Alicyclobacillus acidiphilus NBRC 100859GCA_001544355contigncbi1255277

GC content

@refGC-contentmethod
538454.1
2328554.1high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes99no
motileyes84.866yes
gram-positiveyes88.325yes
anaerobicno98.937no
aerobicyes93.036no
halophileno90.937no
spore-formingyes94.523yes
thermophileyes96.185yes
glucose-utilyes87.455no
flagellatedno69.411no
glucose-fermentno87.878no

External links

@ref: 5384

culture collection no.: DSM 14558, IAM 14935, NRIC 0496, JCM 21417, CIP 107723, NBRC 100859, NRIC 6496

straininfo link

  • @ref: 70094
  • straininfo: 100493

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12361274Alicyclobacillus acidiphilus sp. nov., a novel thermo-acidophilic, omega-alicyclic fatty acid-containing bacterium isolated from acidic beverages.Matsubara H, Goto K, Matsumura T, Mochida K, Iwaki M, Niwa M, Yamasato KInt J Syst Evol Microbiol10.1099/00207713-52-5-16812002Bacteria, Aerobic/*classification/genetics/*isolation & purification/metabolism, Base Composition, Beverages/microbiology, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis/chemistry, Gram-Positive Endospore-Forming Rods/*classification/genetics/*isolation & purification/metabolism, Guaiacol/metabolism, Hydrogen-Ion Concentration, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Terminology as TopicMetabolism
Biotechnology20338657Kinetics models for the inactivation of Alicyclobacillus acidiphilus DSM14558(T) and Alicyclobacillus acidoterrestris DSM 3922(T) in apple juice by ultrasound.Wang J, Hu X, Wang ZInt J Food Microbiol10.1016/j.ijfoodmicro.2010.02.0292010Alicyclobacillus/*growth & development, Beverages/*microbiology, Food Contamination/prevention & control, *Food Microbiology, Linear Models, Malus/*microbiology, Microbial Viability, Models, Theoretical, *UltrasonicsEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5384Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14558)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14558
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23285Hiroshige Matsubara, Keiichi Goto, Terumi Matsumura, Kaoru Mochida, Masaharu Iwaki, Motohiro Niwa, Kazuhide Yamasato10.1099/00207713-52-5-1681Alicyclobacillus acidiphilus sp. nov., a novel thermo-acidophilic, omega-alicyclic fatty acid-containing bacterium isolated from acidic beverages.IJSEM 52: 1681-1685 200212361274
38174Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5190
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70094Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100493.1StrainInfo: A central database for resolving microbial strain identifiers
116003Curators of the CIPCollection of Institut Pasteur (CIP 107723)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107723