Name: Olsenella profusa D315A-29
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 3060

Type strain:

Species: Olsenella profusa

Strain Designation: D315A-29

Culture col. no.: CIP 106885, JCM 14553, DSM 13989, CCUG 45371, KCTC 15029

Strain history: CIP <- 2001, J. Downes, Oral Microbiol. Unit, London, UK: strain D315A-29 <- Kings College, London, UK <- Forsyth Dental Inst., Boston, USA <- W.E.C. Moore, USA: strain D52, Eubacterium

NCBI tax ID(s): 997349 (strain), 138595 (species)

SI-ID 49542

DSM 13989, CIP 106885, KCTC 13917, CCUG 45371, JCM 14553, KCTC 15029

Other strains from Olsenella profusa

O. profusa CCUG 45372

Section

Olsenella profusa D315A-29 is an obligate anaerobe, mesophilic, Gram-positive bacterium that was isolated from subgingival plaque.

Gram-positive
oval-shaped
mesophilic
obligate anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Coriobacteriia*
	Order	*Coriobacteriales*
	Family	*Atopobiaceae*
	Genus	*Olsenella*
	Species	**Olsenella profusa**
	Full Scientific Name (LPSN)	Olsenella profusa Dewhirst et al. 2001

Information on morphological and physiological properties Morphology

[Ref.: #121961]	Gram stain	positive

[Ref.: #121961]	Cell shape	oval-shaped

[Ref.: #121961]	Motility	no

[Ref.: #121961]

Hemolysis ability

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5207]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #5207]

Culture medium growth

[Ref.: #5207]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5207]

Culture medium composition

expand / minimize

[Ref.: #38652]

Culture medium

MEDIUM 6 - Columbia agar with 10 % horse blood

[Ref.: #38652]

Culture medium growth

[Ref.: #38652]

Culture medium composition

expand / minimize

[Ref.: #121961]

Culture medium

CIP Medium 6

[Ref.: #121961]

Culture medium growth

[Ref.: #121961]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #5207]	growth	37 °C
[Ref.: #38652]	growth	37 °C
[Ref.: #56749]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #5207]	Temperature range	mesophilic
[Ref.: #38652]	Temperature range	mesophilic
[Ref.: #56749]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #56749]	Oxygen tolerance	obligate anaerobe
[Ref.: #121961]	Oxygen tolerance	anaerobe

[Ref.: #5207]	Murein short key	A11.31
[Ref.: #5207]	Murein types	A4alpha L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #121961]	cellobiose ChEBI	+	degradation
[Ref.: #121961]	D-arabinose ChEBI	+	degradation
[Ref.: #121961]	D-fructose ChEBI	+	degradation
[Ref.: #121961]	D-glucose ChEBI	+	degradation
[Ref.: #121961]	D-xylose ChEBI	+	degradation
[Ref.: #121961]	esculin ChEBI	+	hydrolysis
[Ref.: #121961]	lactose ChEBI	+	degradation
[Ref.: #121961]	maltose ChEBI	+	degradation
[Ref.: #121961]	nitrate ChEBI	-	reduction
[Ref.: #121961]	nitrate ChEBI	+	respiration
[Ref.: #121961]	nitrite ChEBI	-	reduction
[Ref.: #121961]	salicin ChEBI	+	degradation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #121961]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121961]	amylase	-
[Ref.: #121961]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121961]	caseinase	-	3.4.21.50
[Ref.: #121961]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #121961]	DNase	-
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #121961]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121961]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121961]	tween esterase	-
[Ref.: #121961]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121961]	9889	-	-	-	-	-	+	-	+	-	-	+	+	-	-	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5207]	Sample type/isolated from	subgingival plaque
[Ref.: #5207]	Country	USA
[Ref.: #5207]	Country ISO 3 Code	USA
[Ref.: #5207]	Continent	North America

[Ref.: #56749]	Sample type/isolated from	Subgingival plaque in adult with periodontitis

[Ref.: #67770]	Sample type/isolated from	Human subgingival plaque

[Ref.: #121961]	Sample type/isolated from	Human, Subgingival plaque
[Ref.: #121961]	Country	United States of America
[Ref.: #121961]	Country ISO 3 Code	USA
[Ref.: #121961]	Continent	North America
[Ref.: #121961]	Isolation date	1993
* marker position based on {}

Isolation sources categories

#Host Body-Site

#Oral cavity and airways

#Subgingival plaque

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC258149 (>99% sequence identity) for Olsenella profusa subclade from Microbeatlas

Aquatic Samples	185
Soil Samples	69
Animal Samples	4478
Plant Samples	43
Total Samples	4775

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5207]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121961]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5207]	Olsenella profusa 16S ribosomal RNA gene, partial sequence	AF292374	1437	ENA	997349 tax ID
[Ref.: #67770]	Olsenella profusa gene for 16S ribosomal RNA, partial sequence, strain: JCM 14553	LC258149	1453	ENA	138595 tax ID
[Ref.: #20218]	Olsenella profusa partial 16S rRNA gene, type strain DSM 13989T	FN178465	1428	ENA	997349 tax ID	*
[Ref.: #20218]	Olsenella profusa partial 16S rRNA gene, type strain CCUG 45371T	FN178466	1428	ENA	997349 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Olsenella profusa DSM 13989	2923489329	draft	JGI IMG/M	997349 tax ID	*

[Ref.: #5207]	GC-content	64 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	64.5 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #5207]

Culture collection no.

CIP 106885, JCM 14553, DSM 13989, CCUG 45371, KCTC 15029

[Ref.: #72580]

SI-ID 49542

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Reclassification of Olsenella gallinarum as Thermophilibacter gallinarum comb. nov. and description of Thermophilibacter immobilis sp. nov., isolated from the mud in a fermentation cellar used for the production of Chinese Luzhou-flavour Baijiu.	Lu LF, Yang Y, Zheng L, Zhang R, Liu GQ, Tu TY, Xu T, Luo X, Ran MF, Zhang LQ, Wang ST, Shen CH, Zhang YG	Int J Syst Evol Microbiol	10.1099/ijsem.0.005192	2021	*
Phylogeny	Taxono-genomics description of Olsenella lakotia SW165 (T) sp. nov., a new anaerobic bacterium isolated from cecum of feral chicken.	Wongkuna S, Ghimire S, Janvilisri T, Doerner K, Chankhamhaengdecha S, Scaria J	F1000Res	10.12688/f1000research.25823.1	2020	*
Phylogeny	Olsenella faecalis sp. nov., an anaerobic actinobacterium isolated from human faeces.	Han KI, Lee KC, Eom MK, Kim JS, Suh MK, Park SH, Lee JH, Kang SW, Park JE, Oh BS, Yu SY, Choi SH, Lee DH, Yoon H, Kim BY, Yang SJ, Lee JS	Int J Syst Evol Microbiol	10.1099/ijsem.0.003469	2019	*
Phylogeny	Parolsenella catena gen. nov., sp. nov., isolated from human faeces.	Sakamoto M, Iino T, Hamada M, Ohkuma M	Int J Syst Evol Microbiol	10.1099/ijsem.0.002645	2018	*
Phylogeny	Olsenella scatoligenes sp. nov., a 3-methylindole- (skatole) and 4-methylphenol- (p-cresol) producing bacterium isolated from pig faeces.	Li X, Jensen RL, Hojberg O, Canibe N, Jensen BB	Int J Syst Evol Microbiol	10.1099/ijs.0.000083	2015	*

References

#5207

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13989

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#38652 ; Curators of the CIP;
#56749 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 45371

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#72580

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID49542.1 )

#121961 Collection of Institut Pasteur ; Curators of the CIP; CIP 106885
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Olsenella profusa in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF