Strain identifier

BacDive ID: 3060

Type strain: Yes

Species: Olsenella profusa

Strain Designation: D315A-29

Strain history: CIP <- 2001, J. Downes, Oral Microbiol. Unit, London, UK: strain D315A-29 <- Kings College, London, UK <- Forsyth Dental Inst., Boston, USA <- W.E.C. Moore, USA: strain D52, Eubacterium

NCBI tax ID(s): 997349 (strain), 138595 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 5207

BacDive-ID: 3060

DSM-Number: 13989

keywords: genome sequence, 16S sequence, Bacteria, obligate anaerobe, mesophilic, Gram-positive, oval-shaped

description: Olsenella profusa D315A-29 is an obligate anaerobe, mesophilic, Gram-positive bacterium that was isolated from subgingival plaque.

NCBI tax id

NCBI tax idMatching level
997349strain
138595species

strain history

@refhistory
5207<- J. Downes; D315A-29 <- Forsyth Dental Institute, Boston <- VPI (Eubacterium D52)
67770CCUG 45371 <-- J. Downes D315A-29 <-- A. Tanner <-- W. E. C. Moore D52.
121961CIP <- 2001, J. Downes, Oral Microbiol. Unit, London, UK: strain D315A-29 <- Kings College, London, UK <- Forsyth Dental Inst., Boston, USA <- W.E.C. Moore, USA: strain D52, Eubacterium

doi: 10.13145/bacdive3060.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Coriobacteriia
  • order: Coriobacteriales
  • family: Atopobiaceae
  • genus: Olsenella
  • species: Olsenella profusa
  • full scientific name: Olsenella profusa Dewhirst et al. 2001

@ref: 5207

domain: Bacteria

phylum: Actinobacteria

class: Coriobacteriia

order: Coriobacteriales

family: Atopobiaceae

genus: Olsenella

species: Olsenella profusa

full scientific name: Olsenella profusa Dewhirst et al. 2001 emend. Kraatz et al. 2011

strain designation: D315A-29

type strain: yes

Morphology

cell morphology

  • @ref: 121961
  • gram stain: positive
  • cell shape: oval-shaped
  • motility: no

colony morphology

  • @ref: 121961
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5207PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
38652MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
121961CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
5207positivegrowth37mesophilic
38652positivegrowth37mesophilic
56749positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
56749obligate anaerobe
121961anaerobe

murein

  • @ref: 5207
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12196117108D-arabinose+degradation
12196115824D-fructose+degradation
12196117634D-glucose+degradation
12196165327D-xylose+degradation
12196117057cellobiose+degradation
12196117716lactose+degradation
12196117306maltose+degradation
12196117814salicin+degradation
1219614853esculin+hydrolysis
12196117632nitrate-reduction
12196116301nitrite-reduction
12196117632nitrate+respiration

metabolite production

  • @ref: 121961
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
121961oxidase-
121961beta-galactosidase+3.2.1.23
121961gelatinase-
121961amylase-
121961DNase-
121961caseinase-3.4.21.50
121961catalase-1.11.1.6
121961tween esterase-
121961urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121961-----+-+--++---++---

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
5207subgingival plaqueUSAUSANorth America
56749Subgingival plaque in adult with periodontitis
67770Human subgingival plaque
121961Human, Subgingival plaqueUnited States of AmericaUSANorth America1993

isolation source categories

  • Cat1: #Host Body-Site
  • Cat2: #Oral cavity and airways
  • Cat3: #Subgingival plaque

taxonmaps

  • @ref: 69479
  • File name: preview.99_7817.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_150;96_1373;97_1633;98_5713;99_7817&stattab=map
  • Last taxonomy: Olsenella profusa subclade
  • 16S sequence: LC258149
  • Sequence Identity:
  • Total samples: 4775
  • soil counts: 69
  • aquatic counts: 185
  • animal counts: 4478
  • plant counts: 43

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
52072Risk group (German classification)
1219611Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Olsenella profusa partial 16S rRNA gene, type strain DSM 13989TFN1784651428ena997349
20218Olsenella profusa partial 16S rRNA gene, type strain CCUG 45371TFN1784661428ena997349
5207Olsenella profusa 16S ribosomal RNA gene, partial sequenceAF2923741437ena997349
67770Olsenella profusa gene for 16S ribosomal RNA, partial sequence, strain: JCM 14553LC2581491453ena138595

Genome sequences

  • @ref: 66792
  • description: Olsenella profusa DSM 13989
  • accession: 2923489329
  • assembly level: draft
  • database: img
  • NCBI tax ID: 997349

GC content

@refGC-contentmethod
520764high performance liquid chromatography (HPLC)
6777064.5high performance liquid chromatography (HPLC)

External links

@ref: 5207

culture collection no.: CIP 106885, JCM 14553, DSM 13989, CCUG 45371, KCTC 15029

straininfo link

  • @ref: 72580
  • straininfo: 49542

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny25634945Olsenella scatoligenes sp. nov., a 3-methylindole- (skatole) and 4-methylphenol- (p-cresol) producing bacterium isolated from pig faeces.Li X, Jensen RL, Hojberg O, Canibe N, Jensen BBInt J Syst Evol Microbiol10.1099/ijs.0.0000832015Actinobacteria/*classification/genetics/isolation & purification, Animals, Bacterial Typing Techniques, Base Composition, Cresols/*metabolism, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/microbiology, Fermentation, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Skatole/*metabolism, Sus scrofa/*microbiologyMetabolism
Phylogeny29458507Parolsenella catena gen. nov., sp. nov., isolated from human faeces.Sakamoto M, Iino T, Hamada M, Ohkuma MInt J Syst Evol Microbiol10.1099/ijsem.0.0026452018Actinobacteria/*classification/genetics/isolation & purification, Adult, Bacteria, Anaerobic/classification/genetics/isolation & purification, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/*microbiology, Genes, Bacterial, Humans, Japan, Male, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAEnzymology
Phylogeny31135332Olsenella faecalis sp. nov., an anaerobic actinobacterium isolated from human faeces.Han KI, Lee KC, Eom MK, Kim JS, Suh MK, Park SH, Lee JH, Kang SW, Park JE, Oh BS, Yu SY, Choi SH, Lee DH, Yoon H, Kim BY, Yang SJ, Lee JSInt J Syst Evol Microbiol10.1099/ijsem.0.0034692019Actinobacteria/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Feces/*microbiology, Glycolipids/chemistry, Humans, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNAPathogenicity
Phylogeny33024551Taxono-genomics description of Olsenella lakotia SW165 (T) sp. nov., a new anaerobic bacterium isolated from cecum of feral chicken.Wongkuna S, Ghimire S, Janvilisri T, Doerner K, Chankhamhaengdecha S, Scaria JF1000Res10.12688/f1000research.25823.12020Actinobacteria, Anaerobiosis, Animals, Bacterial Typing Techniques, Base Composition, Cecum, *Chickens, DNA, Bacterial, *Genomics, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
Phylogeny34914571Reclassification of Olsenella gallinarum as Thermophilibacter gallinarum comb. nov. and description of Thermophilibacter immobilis sp. nov., isolated from the mud in a fermentation cellar used for the production of Chinese Luzhou-flavour Baijiu.Lu LF, Yang Y, Zheng L, Zhang R, Liu GQ, Tu TY, Xu T, Luo X, Ran MF, Zhang LQ, Wang ST, Shen CH, Zhang YGInt J Syst Evol Microbiol10.1099/ijsem.0.0051922021Actinobacteria/*classification/isolation & purification, Alcoholic Beverages, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, *Fatty Acids/chemistry, *Fermentation, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil MicrobiologyTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5207Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13989)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13989
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38652Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19064
56749Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 45371)https://www.ccug.se/strain?id=45371
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
72580Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49542.1StrainInfo: A central database for resolving microbial strain identifiers
121961Curators of the CIPCollection of Institut Pasteur (CIP 106885)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106885