Name: Olsenella umbonata A2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 3061

Type strain:

Species: Olsenella umbonata

Strain Designation: A2

Culture col. no.: DSM 22619, CCUG 58212, JCM 16157

Strain history: M. Kraatz A2 <-- J. R. Wallace.

SI-ID 402570

DSM 22619, CCUG 58212

Other strains from Olsenella umbonata

O. umbonata lac31, DSM 22620, CCUG 58604, JCM ... (type strain)
O. umbonata CA-693-APC-4B, DSM 105184
O. umbonata WB01-CNA04-AN, DSM 105334

Section

Olsenella umbonata A2 is an obligate anaerobe, mesophilic bacterium that was isolated from ruminal fluid of a rumen-fistulated sheep.

mesophilic
obligate anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Coriobacteriia*
	Order	*Coriobacteriales*
	Family	*Atopobiaceae*
	Genus	*Olsenella*
	Species	**Olsenella umbonata**
	Full Scientific Name (LPSN)	Olsenella umbonata Kraatz et al. 2011

Synonym

Parafannyhessea umbonata

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %99.999

[Ref.: #69480]	Motility	no Confidence in %94.206

[Ref.: #61631]

Incubation period

1-4 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16368]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #16368]

Culture medium growth

[Ref.: #16368]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16368]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #16368]	growth	37 °C
[Ref.: #61631]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #16368]	Temperature range	mesophilic
[Ref.: #61631]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #16368]	Oxygen tolerance	obligate anaerobe
[Ref.: #61631]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.999

[Ref.: #69481]	Ability of spore formation	no Confidence in %97
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.989

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	+	hydrolysis	* from API rID32A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	+	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	-	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	+		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #16368]	12768	-	+	-	-	-	+	-	-	-	-	+/-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16368]	Sample type/isolated from	ruminal fluid of a rumen-fistulated sheep
[Ref.: #16368]	Geographic location (country and/or sea, region)	Scotland, Aberdeen, Rowett Research Institute
[Ref.: #16368]	Country	United Kingdom
[Ref.: #16368]	Country ISO 3 Code	GBR
[Ref.: #16368]	Continent	Europe

[Ref.: #61631]	Sample type/isolated from	Sheep ruminal fluid,rumenfistulated sheep
[Ref.: #61631]	Sampling date	2004-07-01
[Ref.: #61631]	Geographic location (country and/or sea, region)	Scotland,Aberdeen,Rowett Research Ins
[Ref.: #61631]	Country	United Kingdom
[Ref.: #61631]	Country ISO 3 Code	GBR
[Ref.: #61631]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Ruminal fluid of a sheep
[Ref.: #67770]	Geographic location (country and/or sea, region)	Aberdeen
[Ref.: #67770]	Country	United Kingdom
[Ref.: #67770]	Country ISO 3 Code	GBR
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Caprinae (Sheep/Goat)
#Host Body-Site	#Organ	#Rumen
#Host Body Product	#Fluids	#Rumen fluid

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ251324 (>99% sequence identity) for Olsenella umbonata subclade from Microbeatlas

Aquatic Samples	902
Soil Samples	512
Animal Samples	12522
Plant Samples	255
Total Samples	14191

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16368]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16368]	Olsenella umbonata partial 16S rRNA gene, strain A2	AJ251324	1433	ENA	604330 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Parafannyhessea umbonata DSM 22619	GCA_900101655	contig	GenBank	604330 tax ID
[Ref.: #66792]	Olsenella umbonata strain DSM 22619	604330.4	wgs	PATRIC	604330 tax ID	*
[Ref.: #66792]	Parafannyhessea umbonata DSM 22619	2606217797	draft	JGI IMG/M	604330 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Olsenella umbonata strain DSM 22619 PATRIC: 604330.4
[Ref.: #69481]	spore-forming	no	97	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Olsenella umbonata strain DSM 22619 PATRIC: 604330.4
[Ref.: #69480]	gram-positive	yes	93.806	no
[Ref.: #69480]	anaerobic	yes	99.048	yes
[Ref.: #69480]	halophile	no	84.033	no
[Ref.: #69480]	spore-forming	no	94.632	no
[Ref.: #69480]	glucose-util	yes	86.017	no
[Ref.: #69480]	thermophile	no	91.166	yes
[Ref.: #69480]	flagellated	no	97.017	no
[Ref.: #69480]	aerobic	no	97.252	no
[Ref.: #69480]	motile	no	89.266	no
[Ref.: #69480]	glucose-ferment	yes	78.891	no

Availability in culture collections External links

[Ref.: #16368]

Culture collection no.

DSM 22619, CCUG 58212, JCM 16157

[Ref.: #72581]

SI-ID 402570

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Olsenella umbonata sp. nov., a microaerotolerant anaerobic lactic acid bacterium from the sheep rumen and pig jejunum, and emended descriptions of Olsenella, Olsenella uli and Olsenella profusa.	Kraatz M, Wallace RJ, Svensson L	Int J Syst Evol Microbiol	10.1099/ijs.0.022954-0	2010	*

References

#16368

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22619

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#61631 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 58212

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#72581

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402570.1 )

* These data were automatically processed and therefore are not curated

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Olsenella umbonata strain DSM 22619

Olsenella umbonata strain DSM 22619

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