Ottowia thiooxydans K11 | Type strain | DSM 14619, JCM 11629 | BacDiveID:2979

Strain identifier

BacDive ID: 2979

Type strain:

Species: Ottowia thiooxydans

Strain Designation: K11

Culture col. no.: DSM 14619, JCM 11629

Strain history: S. Spring K11 <-- P. Kämpfer <-- M. Wagner.

NCBI tax ID(s): 1122623 (strain), 219182 (species)

SI-ID 130849

DSM 14619, JCM 11629, CCUG 56032, CCUG 56058

Section

Ottowia thiooxydans K11 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from activated sludge.

Gram-negative
rod-shaped
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Comamonadaceae*
	Genus	*Ottowia*
	Species	**Ottowia thiooxydans**
	Full Scientific Name (LPSN)	Ottowia thiooxydans Spring et al. 2004

Information on morphological and physiological properties Morphology

[Ref.: #29945]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.998

[Ref.: #29945]	Cell length	4 µm

[Ref.: #29945]	Cell width	0.8 µm

[Ref.: #29945]	Cell shape	rod-shaped

[Ref.: #29945]	Motility	no

Pigment production

yes

Multimedia content

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Information on culture and growth conditions Culture and growth conditions

Culture medium

REACTIVATION WITH LIQUID MEDIUM (DSMZ Medium 535b)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

Culture medium

REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

Culture medium

REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #5412]			growth	25 °C
[Ref.: #29945]			growth	02-29 °C
[Ref.: #29945]			optimum	26 °C
[Ref.: #67770]			growth	26 °C

[Ref.: #5412]	Temperature range	mesophilic
[Ref.: #29945]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29945]			optimum	7.3
[Ref.: #29945]			growth	5.6-9.8

Information on physiology and metabolism Physiology and metabolism

Oxygen tolerance

facultative anaerobe

[Ref.: #29945]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.996

[Ref.: #29945]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: Q-8

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29945]		3-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #29945]		acetate ChEBI	+	carbon source
[Ref.: #29945]		aspartate ChEBI	+	carbon source
[Ref.: #29945]		lactate ChEBI	+	carbon source
[Ref.: #29945]		leucine ChEBI	+	carbon source
[Ref.: #29945]		malate ChEBI	+	carbon source
[Ref.: #29945]		nitrate ChEBI	+	reduction
[Ref.: #29945]		phenylacetate ChEBI	+	carbon source
[Ref.: #29945]		phenylalanine ChEBI	+	carbon source
[Ref.: #29945]		proline ChEBI	+	carbon source
[Ref.: #29945]		propionate ChEBI	+	carbon source
[Ref.: #29945]		pyruvate ChEBI	+	carbon source
[Ref.: #29945]		suberic acid ChEBI	+	carbon source
		Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #29945]		catalase	+	1.11.1.6
[Ref.: #29945]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5412]	Sample type/isolated from	activated sludge
[Ref.: #5412]	Geographic location (country and/or sea, region)	Munich
[Ref.: #5412]	Country	Germany
[Ref.: #5412]	Country ISO 3 Code	DEU
[Ref.: #5412]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Activated sludge of a municipal wastewater treatment plant
[Ref.: #67770]	Geographic location (country and/or sea, region)	Grosslappen
[Ref.: #67770]	Country	Germany
[Ref.: #67770]	Country ISO 3 Code	DEU
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered

#Waste

#Activated sludge

What are isolation sources categories?

Global distribution of 16S sequence LC373193 (>99% sequence identity) for Ottowia thiooxydans subclade from Microbeatlas

Aquatic Samples	274
Soil Samples	27
Animal Samples	116
Plant Samples	13
Total Samples	430

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

Biosafety level

1

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #29945]	Ottowia thiooxidans partial 16S rRNA gene, type strain K11T	AJ537466	1525	GenBank	219182 tax ID
[Ref.: #67770]	Ottowia thiooxydans JCM 11629 gene for 16S ribosomal RNA, partial sequence	LC373193	1470	ENA	219182 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Ottowia thiooxydans DSM 14619	GCA_000422885	scaffold	GenBank	1122623 tax ID
[Ref.: #66792]	Ottowia thiooxydans DSM 14619	1122623.5	wgs	PATRIC	1122623 tax ID	*
[Ref.: #66792]	Ottowia thiooxydans DSM 14619	2523533611	draft	JGI IMG/M	1122623 tax ID	*

[Ref.: #5412]	GC-content	59.3 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #29945]	GC-content	59 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Ottowia thiooxydans DSM 14619 DSM 14619 NCBI: GCA_000422885.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Ottowia thiooxydans DSM 14619 PATRIC: 1122623.5
[Ref.: #69480]	motile	no	65.736	no
[Ref.: #69480]	flagellated	no	91.833	yes
[Ref.: #69480]	gram-positive	no	97.956	yes
[Ref.: #69480]	anaerobic	no	98.617	no
[Ref.: #69480]	aerobic	yes	94.449	no
[Ref.: #69480]	halophile	no	94.558	no
[Ref.: #69480]	spore-forming	no	93.65	yes
[Ref.: #69480]	glucose-util	no	76.507	no
[Ref.: #69480]	thermophile	no	99.727	yes
[Ref.: #69480]	glucose-ferment	no	91.1	no

Availability in culture collections External links

Culture collection no.

DSM 14619, JCM 11629

SI-ID 130849

*

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Ottowia testudinis sp. nov., isolated from the cloaca of a giant Asian pond turtle (Heosemys grandis).	Busse HJ, Kampfer P, Szostak MP, Spergser J	Int J Syst Evol Microbiol	10.1099/ijsem.0.005348	2022	*
Phylogeny	Ottowia caeni sp. nov., a novel phenylacetic acid degrading bacterium isolated from sludge.	Pang FH, Yang HY, Sun J, Yu X, Zhang H	Int J Syst Evol Microbiol	10.1099/ijsem.0.005144	2021	*
Phylogeny	Ottowia flava sp. nov., isolated from fish intestines.	Shi SB, Li GD, Yang LF, Liu C, Jiang MG, Li QY, Wu JF, Zhang K, Jiang LQ, Shen NK, Jiang CL, Jiang Y	Antonie Van Leeuwenhoek	10.1007/s10482-019-01284-6	2019	*
Phylogeny	Ottowia oryzae sp. nov., isolated from Andong sikhye, a Korean traditional rice beverage.	Heo J, Cho H, Hong SB, Kim JS, Kwon SW, Kim SJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.002935	2018	*
Phylogeny	Ottowia thiooxydans gen. nov., sp. nov., a novel facultatively anaerobic, N2O-producing bacterium isolated from activated sludge, and transfer of Aquaspirillum gracile to Hylemonella gracilis gen. nov., comb. nov.	Spring S, Jackel U, Wagner M, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.02727-0	2004	*

References

#5412

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14619

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29945

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26314 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66793

Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#72501

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID130849.1 )

#26314

IJSEM 99 2004 ( DOI 10.1099/ijs.0.02727-0 , PubMed 14742465 )

* These data were automatically processed and therefore are not curated

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