Ottowia thiooxydans (DSM 14619, JCM 11629, K11)

Name: Ottowia thiooxydans (DSM 14619, JCM 11629, K11)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2979

Type strain

Strain Designation K11

Culture col. no. DSM 14619 JCM 11629 K11

NCBI tax ID(s) 1122623 219182

Special Collections DSMZ JCM Literature strains

Links

Ottowia thiooxydans K11 is a facultative anaerobe, mesophilic, Gram-negative prokaryote that was isolated from activated sludge.

Gram-negative rod-shaped facultative anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 130849

DSM 14619,JCM 11629,CCUG 56032,CCUG 56058

@ref 20215

Last LPSN update 2025-12-16 09:47:26

Domain Pseudomonadati

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Comamonadaceae

Genus Ottowia

Species Ottowia thiooxydans

Full scientific name Ottowia thiooxydans Spring et al. 2004

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
29945	negative	4 µm	0.8 µm	rod-shaped
125438	negative				96.625
125439	negative				98.4

Pigmentation

29945

Productionyes

Multimedia

@ref:	66793
multimedia content:	EM_DSM_14619_1.jpg
multimedia.multimedia content:	EM_DSM_14619_1.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_14619_2.jpg
multimedia.multimedia content:	EM_DSM_14619_2.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_14619_3.jpg
multimedia.multimedia content:	EM_DSM_14619_3.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_14619_4.jpg
multimedia.multimedia content:	EM_DSM_14619_4.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_14619_5.jpg
multimedia.multimedia content:	EM_DSM_14619_5.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5412	REACTIVATION WITH LIQUID MEDIUM (DSMZ Medium 535b)	Medium recipe at MediaDive	Name: REACTIVATION WITH LIQUID MEDIUM (DSMZ Medium 535b) Composition: Trypticase soy broth 30.0 g/l Casein peptone 17.0 g/l Agar 15.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l K2HPO4 2.5 g/l D(+)-Glucose 2.5 g/l Distilled water
5412	REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c)	Medium recipe at MediaDive	Name: REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c) Composition: Agar 15.0 g/l Yeast extract 0.5 g/l Proteose peptone 0.5 g/l Casamino acids 0.5 g/l Glucose 0.5 g/l Starch 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
5412	REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a)	Medium recipe at MediaDive	Name: REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a) Composition: Casein peptone 17.0 g/l Agar 15.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
5412	positive	growth	25	mesophilic
29945	positive	growth	02-29
29945	positive	optimum	26	mesophilic
67770	positive	growth	26	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
29945	positive	optimum	7.3
29945	positive	growth	5.6-9.8	alkaliphile

Physiology and metabolism

Oxygen tolerance

29945

Oxygen tolerancefacultative anaerobe

Spore formation

@ref	Spore formation	Confidence
29945
125439		98.7

Observation

@ref	Observation
29945	aggregates in chains
67770	quinones: Q-8

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
29945	37054 ChEBI	3-hydroxybutyrate	+	carbon source
29945	30089 ChEBI	acetate	+	carbon source
29945	35391 ChEBI	aspartate	+	carbon source
29945	24996 ChEBI	lactate	+	carbon source
29945	25017 ChEBI	leucine	+	carbon source
29945	25115 ChEBI	malate	+	carbon source
29945	17632 ChEBI	nitrate	+	reduction
29945	18401 ChEBI	phenylacetate	+	carbon source
29945	28044 ChEBI	phenylalanine	+	carbon source
29945	26271 ChEBI	proline	+	carbon source
29945	17272 ChEBI	propionate	+	carbon source
29945	15361 ChEBI	pyruvate	+	carbon source
29945	9300 ChEBI	suberic acid	+	carbon source

Enzymes

@ref	Value	Activity	Ec
29945	catalase	+	1.11.1.6
29945	cytochrome oxidase	+	1.9.3.1

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Waste	#Activated sludge

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
5412	activated sludge	Munich	Germany	DEU	Europe
67770	Activated sludge of a municipal wastewater treatment plant	Grosslappen	Germany	DEU	Europe

Taxonmaps

69479

Global distribution of 16S sequence LC373193 (>99% sequence identity) for Ottowia thiooxydans subclade from Microbeatlas

Aquatic Samples	274
Soil Samples	27
Animal Samples	116
Plant Samples	13
Total Samples	430

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5412	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM42288v1 assembly for Ottowia thiooxydans DSM 14619	scaffold	GCA_000422885	1122623.5	2523533611	1122623	60.53

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
29945	Ottowia thiooxidans partial 16S rRNA gene, type strain K11T	AJ537466	1525		219182
67770	Ottowia thiooxydans JCM 11629 gene for 16S ribosomal RNA, partial sequence	LC373193	1470		219182

GC content

@ref	GC-content (mol%)	Method
5412	59.3	thermal denaturation, midpoint method (Tm)
29945	59

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Ottowia thiooxydans DSM 14619
NCBI: GCA_000422885

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	98.70	no
125439	motility	BacteriaNetⓘ	yes	74.60	no
125439	gram_stain	BacteriaNetⓘ	negative	98.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	85.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Ottowia thiooxydans DSM 14619
PATRIC: 1122623.5

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	96.63	yes
125438	anaerobic	anaerobicⓘ	no	95.29	no
125438	spore-forming	spore-formingⓘ	no	83.19	no
125438	aerobic	aerobicⓘ	yes	77.49	yes
125438	thermophilic	thermophileⓘ	no	97.94	no
125438	flagellated	motile2+ⓘ	yes	53.98	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
	Identification of NpdA as the protein forming the surface layer in Paracidovorax citrulli and evidence of its occurrence as a surface layer protein in diverse genera of the Betaproteobacteria and Gammaproteobacteria.	Gajbhiye S, Gonzales ED, Toso DB, Kirk NA, Hickey WJ.	Access Microbiol	10.1099/acmi.0.000685.v3	2023
Enzymology	Protocatechuate hydroxylase is a novel group A flavoprotein monooxygenase with a unique substrate recognition mechanism.	Katsuki N, Fukushima R, Doi Y, Masuo S, Arakawa T, Yamada C, Fushinobu S, Takaya N.	J Biol Chem	10.1016/j.jbc.2023.105508	2024
Phylogeny	Ottowia shaoguanensis sp. nov., isolated from coking wastewater.	Geng S, Pan XC, Mei R, Wang YN, Sun JQ, Liu XY, Tang YQ, Wu XL.	Curr Microbiol	10.1007/s00284-013-0481-8	2014
Phylogeny	Ottowia beijingensis sp. nov., isolated from coking wastewater activated sludge, and emended description of the genus Ottowia.	Cao J, Lai Q, Liu Y, Li G, Shao Z.	Int J Syst Evol Microbiol	10.1099/ijs.0.054015-0	2014
Phylogeny	Ottowia cancrivicina sp. nov., isolated from human stomach.	Song Y, Yuan XY, Cong HY, Liu Y, Liu P, Wang MY, Du ZJ.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006360	2024
Phylogeny	Ottowia testudinis sp. nov., isolated from the cloaca of a giant Asian pond turtle (Heosemys grandis).	Busse HJ, Kampfer P, Szostak MP, Spergser J	Int J Syst Evol Microbiol	10.1099/ijsem.0.005348	2022
Phylogeny	Ottowia caeni sp. nov., a novel phenylacetic acid degrading bacterium isolated from sludge.	Pang FH, Yang HY, Sun J, Yu X, Zhang H	Int J Syst Evol Microbiol	10.1099/ijsem.0.005144	2021
Phylogeny	Ottowia flava sp. nov., isolated from fish intestines.	Shi SB, Li GD, Yang LF, Liu C, Jiang MG, Li QY, Wu JF, Zhang K, Jiang LQ, Shen NK, Jiang CL, Jiang Y	Antonie Van Leeuwenhoek	10.1007/s10482-019-01284-6	2019
Phylogeny	Ottowia oryzae sp. nov., isolated from Andong sikhye, a Korean traditional rice beverage.	Heo J, Cho H, Hong SB, Kim JS, Kwon SW, Kim SJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.002935	2018
Phylogeny	Ottowia thiooxydans gen. nov., sp. nov., a novel facultatively anaerobic, N2O-producing bacterium isolated from activated sludge, and transfer of Aquaspirillum gracile to Hylemonella gracilis gen. nov., comb. nov.	Spring S, Jackel U, Wagner M, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.02727-0	2004

References

#5412	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14619
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#29945	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26314 (see below)
#66793	Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Ottowia thiooxydans (DSM 14619, JCM 11629, K11)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000422885

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media