Giesbergeria kuznetsovii (DSM 12827, CIP 109213, D-412)

Name: Giesbergeria kuznetsovii (DSM 12827, CIP 109213, D-412)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2960

Type strain

Strain Designation D-412

Culture col. no. DSM 12827 CIP 109213 D-412

NCBI tax ID(s) 314202

Special Collections DSMZ CIP Literature strains

Links

Giesbergeria kuznetsovii D-412 is an aerobe, mesophilic, Gram-negative prokaryote that was isolated from sulphide spring.

Gram-negative motile rod-shaped aerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 49375

DSM 12827,VKM B-2352,CIP 109213

@ref 20215

Last LPSN update 2025-12-16 09:47:26

Domain Pseudomonadati

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Comamonadaceae

Genus Giesbergeria

Species Giesbergeria kuznetsovii

Full scientific name Giesbergeria kuznetsovii Grabovich et al. 2006

Morphology

Cell morphology

@ref	Gram stain	Cell width	Cell shape	Motility
31673	negative	1.2-1.5 µm	rod-shaped
120518	negative		rod-shaped

Pigmentation

31673

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
4842	AQUASPIRILLUM MEDIUM II (DSMZ Medium 1495)	Medium recipe at MediaDive	Name: AQUASPIRILLUM MEDIUM II (DSMZ Medium 1495) Composition: Agar 15.0 g/l Peptone 2.0 g/l Succinic acid 1.0 g/l (NH4)2SO4 1.0 g/l MgSO4 x 7 H2O 1.0 g/l CaCl2 x 2 H2O 0.03 g/l EDTA 0.005 g/l FeSO4 x 6 H2O 0.0022 g/l Biotin 0.0002 g/l CoCl2 x 6 H2O 0.0002 g/l Riboflavin 0.0001 g/l ZnSO4 x 7 H2O 0.0001 g/l Pyridoxine hydrochloride 0.0001 g/l Nicotinic acid 5e-05 g/l Thiamine HCl 5e-05 g/l Para-aminobenzoic acid 5e-05 g/l DL-Pantothenic acid 5e-05 g/l MnCl2 x 4 H2O 3e-05 g/l H3BO3 3e-05 g/l CuCl2 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l Folic acid 2e-05 g/l Vitamin B12 1e-06 g/l Distilled water
4842	SPIRILLUM MEDIUM (DSMZ Medium 37)	Medium recipe at MediaDive	Name: SPIRILLUM MEDIUM (DSMZ Medium 37) Composition: Peptone 10.0 g/l (NH4)2SO4 1.0 g/l Succinic acid 1.0 g/l MgSO4 x 7 H2O 1.0 g/l FeCl3 x 6 H2O 0.002 g/l MnSO4 x H2O 0.002 g/l Distilled water
120518	CIP Medium 65	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
4842	positive	growth	28	mesophilic
31673	positive	growth	07-36
31673	positive	optimum	28	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
31673	positive	optimum	7
31673	positive	growth	6-8.5	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
31673	aerobe
120518	facultative anaerobe

Spore formation

31673

Spore formationno

Observation

31673

Observationaggregates in chains

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31673	30089 ChEBI	acetate	+	carbon source
31673	16449 ChEBI	alanine	+	carbon source
31673	22653 ChEBI	asparagine	+	carbon source
31673	35391 ChEBI	aspartate	+	carbon source
31673	16296 ChEBI	D-tryptophan	+	carbon source
31673	15740 ChEBI	formate	+	carbon source
31673	17754 ChEBI	glycerol	+	carbon source
31673	15428 ChEBI	glycine	+	carbon source
31673	27570 ChEBI	histidine	+	carbon source
31673	24996 ChEBI	lactate	+	carbon source
31673	25017 ChEBI	leucine	+	carbon source
31673	25115 ChEBI	malate	+	carbon source
120518	17632 ChEBI	nitrate	+	reduction
120518	16301 ChEBI	nitrite	-	reduction
31673	18257 ChEBI	ornithine	+	carbon source
31673	28044 ChEBI	phenylalanine	+	carbon source
31673	26271 ChEBI	proline	+	carbon source
31673	17272 ChEBI	propionate	+	carbon source
31673	15361 ChEBI	pyruvate	+	carbon source
31673	17822 ChEBI	serine	+	carbon source
31673	30031 ChEBI	succinate	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
120518	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
120518	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	+	3.2.1.31	from API zym
31673	catalase	+	1.11.1.6
120518	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
31673	cytochrome oxidase	+	1.9.3.1
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
120518	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
120518	ornithine decarboxylase	-	4.1.1.17
120518	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
120518	urease	+	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120518	-	+	+	+	-	-	-	-	-	-	+	+	+	+	+	+	+	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Spring
#Condition	#Sulfuric	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
4842	sulphide spring	The Grimea	Russia	RUS	Europe
120518	Environment, Sulphide spring

Taxonmaps

69479

Global distribution of 16S sequence AY780907 (>99% sequence identity) for Giesbergeria from Microbeatlas

Aquatic Samples	2847
Soil Samples	266
Animal Samples	810
Plant Samples	117
Total Samples	4040

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
4842	1	Risk group (German classification) According to TRBA 466
120518	1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
31673	Giesbergeria kuznetsovii strain D-412 16S ribosomal RNA gene, partial sequence	AY780907	1491		314202

GC content

31673

GC-content (mol%)56.5

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Proposal of Giesbergeria voronezhensis gen. nov., sp. nov. and G. kuznetsovii sp. nov. and reclassification of [Aquaspirillum] anulus, [A.] sinuosum and [A.] giesbergeri as Giesbergeria anulus comb. nov., G. sinuosa comb. nov. and G. giesbergeri comb. nov., and [Aquaspirillum] metamorphum and [A.] psychrophilum as Simplicispira metamorpha gen. nov., comb. nov. and S. psychrophila comb. nov.	Grabovich M, Gavrish E, Kuever J, Lysenko AM, Podkopaeva D, Dubinina G	Int J Syst Evol Microbiol	10.1099/ijs.0.64027-0	2006

References

#4842	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12827
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#31673	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27952 (see below)
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120518	Collection of Institut Pasteur ; Curators of the CIP; CIP 109213
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Giesbergeria kuznetsovii (DSM 12827, CIP 109213, D-412)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

Help

Social Media