Clostridium tyrobutyricum (DSM 663, G33R)

Name: Clostridium tyrobutyricum (DSM 663, G33R)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2735

Type strain

Strain Designation G33R

Culture col. no. DSM 663 G33R

NCBI tax ID(s) 1519

Special Collections DSMZ

History <- H. J. Kutzner, TH Darmstadt, Germany; G33R <- H. J. Kutzner {1963}

Links

Clostridium tyrobutyricum G33R is an anaerobe bacterium that was isolated from emmental cheese.

anaerobe genome sequence Bacteria

Name and taxonomic classification

SI-ID 47250

DSM 663,NCIMB 12573,VTT E-99908

@ref 20215

Last LPSN update 2026-02-09 11:18:21

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Clostridiaceae

Genus Clostridium

Species Clostridium tyrobutyricum

Full scientific name Clostridium tyrobutyricum van Beynum and Pette 1935 (Approved Lists 1980)

BacDive ID	Other strains from **Clostridium tyrobutyricum** (5)	Type strain
2738	C. tyrobutyricum DSM 2637, ATCC 25755, NCIMB 10635, CCUG 48315, ... (type strain)
2736	C. tyrobutyricum W55, DSM 664
2737	C. tyrobutyricum La1, DSM 1460
135616	C. tyrobutyricum CIP 60.31
139499	C. tyrobutyricum CIP 60.51

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
251	PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c)		Medium recipe at MediaDive	Name: PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l Na2CO3 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
251	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
251	anaerobe
125439	anaerobe	98.1

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Dairy product

Isolation

251

Sample typeemmental cheese

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
251	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
66792	ASM1914414v1 assembly for Clostridium tyrobutyricum DSM 663	scaffold	GCA_019144145	1519.82	1519	56.41

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Clostridium tyrobutyricum DSM 663
NCBI: GCA_019144145

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	74.80	no
125439	motility	BacteriaNetⓘ	yes	77.00	no
125439	gram_stain	BacteriaNetⓘ	positive	69.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	98.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Clostridium tyrobutyricum DSM 663
NCBI: GCA_019144145.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	63.42	no
125438	anaerobic	anaerobicⓘ	yes	88.87	no
125438	aerobic	aerobicⓘ	no	92.12	yes
125438	spore-forming	spore-formingⓘ	yes	76.95	no
125438	thermophilic	thermophileⓘ	no	89.19	yes
125438	flagellated	motile2+ⓘ	yes	81.26	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Draft Genome Sequences of 12 Clostridium tyrobutyricum Strains Isolated from Raw Milk and Cheese.	Podrzaj L, Burtscher J, Domig KJ.	Microbiol Resour Announc	10.1128/mra.00735-21	2021
	Leuconostoc lactis strain APC 3969 produces a new variant of cyclic bacteriocin leucocyclicin Q and displays potent anti-Clostridium perfringens activity.	de Farias FM, Soria MC, O'Connor PM, Huang X, Buttimer C, Kamilari E, Deliephan A, Hill D, Fursenko O, Wiese J, Draper L, Stanton C, Hill C, Ross RP.	Sci Rep	10.1038/s41598-025-89450-x	2025
	Specificity of the AMP-6000 Method for Enumerating Clostridium Endospores in Milk.	Burtscher J, Rudavsky T, Zitz U, Domig KJ.	Foods	10.3390/foods13081192	2024
	Strain-Dependent Cheese Spoilage Potential of Clostridium tyrobutyricum.	Podrzaj L, Burtscher J, Kuller F, Domig KJ.	Microorganisms	10.3390/microorganisms8111836	2020
	Characterization of Clostridium tyrobutyricum Strains Using Three Different Typing Techniques.	Burtscher J, Kuller F, Dreier M, Arias-Roth E, Drissner D, Domig KJ.	Microorganisms	10.3390/microorganisms8071057	2020
Metabolism	Identification of Clostridium tyrobutyricum as the causative agent of late blowing in cheese by species-specific PCR amplification.	Klijn N, Nieuwenhof FF, Hoolwerf JD, van der Waals CB, Weerkamp AH.	Appl Environ Microbiol	10.1128/aem.61.8.2919-2924.1995	1995

References

#251	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 663
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Clostridium tyrobutyricum (DSM 663, G33R)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_019144145

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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