Clostridium tetanomorphum (DSM 4474, ATCC 49273, NCTC 543)

Name: Clostridium tetanomorphum (DSM 4474, ATCC 49273, NCTC 543)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2727

Type strain

Strain Designation 259 E.III.

Culture col. no. DSM 4474 ATCC 49273 NCTC 543 259 E.III.

NCBI tax ID(s) 1553

Special Collections DSMZ

History DSMZ <- NCTC; NCTC 543 <- M. Robertson, Lister Institute, London, UK; 259 E.III. {1916}

Links

Clostridium tetanomorphum 259 E.III. is an anaerobe bacterium that produces alcohol and was isolated from septic wound.

alcohol production anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 42396

ATCC 49273,NCTC 543,DSM 4474,BCRC 14534,CCRC 14534

@ref 20215

Last LPSN update 2026-05-29 08:25:32

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Clostridiaceae

Genus Clostridium

Species Clostridium tetanomorphum

Full scientific name Clostridium tetanomorphum (ex Bulloch et al. 1919) Wilde et al. 1989

BacDive ID	Other strains from **Clostridium tetanomorphum** (3)	Type strain
2725	C. tetanomorphum H1, DSM 528, ATCC 15920
2726	C. tetanomorphum 297 VII, DSM 665, NCTC 288
134685	C. tetanomorphum 1772C, CIP 108179

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
1687	WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339)		Medium recipe at MediaDive	Name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water
1687	CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)		Medium recipe at MediaDive	Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
1687	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
1687	anaerobe
125438	anaerobe	90.105
125439	facultative anaerobe	97.049

Compound production

1687

Compoundn butanol

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Disease	-
#Host Body-Site	#Other	#Wound

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
1687	septic wound	United Kingdom	GBR	Europe

Taxonmaps

69479

Global distribution of 16S sequence DQ241819 (>99% sequence identity) for Clostridium tetanomorphum subclade from Microbeatlas

Aquatic Samples	850
Soil Samples	1355
Animal Samples	6449
Plant Samples	176
Total Samples	8830

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
1687	yes, in single cases	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1787321v1 assembly for Clostridium tetanomorphum DSM 4474	contig	GCA_017873215	1553.15	2913338184	1553	67.53

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Clostridium tetanomorphum strain DSM 4474 16S ribosomal RNA gene, partial sequence	DQ911261	320	1553
1687	Clostridium tetanomorphum strain DSM 4474 16S ribosomal RNA gene, partial sequence	DQ241819	1407	1553
124043	Clostridium tetanomorphum strain DSM 4474 16S ribosomal RNA gene, partial sequence.	MZ314719	383	1553
124043	Clostridium tetanomorphum strain DSM 4474 16S ribosomal RNA gene, partial sequence.	MZ340548	851	1553

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1787321v1 assembly for Clostridium tetanomorphum DSM 4474
NCBI: GCA_017873215

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	97.05	no
125439	gram_stain	BacteriaNetⓘ	positive	58.45	no
125439	motility	BacteriaNetⓘ	yes	68.92	no
125439	spore_formation	BacteriaNetⓘ	yes	68.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Clostridium tetanomorphum strain DSM 4474
PATRIC: 1553.15

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	50.73	no
125438	anaerobic	anaerobicⓘ	yes	90.11	no
125438	spore-forming	spore-formingⓘ	yes	76.80	no
125438	thermophilic	thermophileⓘ	no	88.66	yes
125438	aerobic	aerobicⓘ	no	96.64	yes
125438	flagellated	motile2+ⓘ	yes	75.26	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Draft genome sequence of Clostridium tetanomorphum DSM 4474^T isolated from a septic wound.	Watkins A, Cano CW, Hoover E, Peterson N, Spring S, Goker M, Ivanova N, Seshadri R, Van Laar TA.	Microbiol Resour Announc	10.1128/mra.00842-25	2025
Enzymology	Occurrence of ferredoxin:NAD(+) oxidoreductase activity and its ion specificity in several Gram-positive and Gram-negative bacteria.	Hess V, Gallegos R, Jones JA, Barquera B, Malamy MH, Muller V.	PeerJ	10.7717/peerj.1515	2016
	Formation of n-Butanol from d-Glucose by Strains of the "Clostridium tetanomorphum" Group.	Gottwald M, Hippe H, Gottschalk G.	Appl Environ Microbiol	10.1128/aem.48.3.573-576.1984	1984
Genetics	Description of Clostridium cagae sp. nov., Clostridium rectalis sp. nov. and Hathewaya massiliensis sp. nov., new anaerobic bacteria isolated from human stool samples.	Tall ML, Lo CI, Yimagou EK, Ndongo S, Pham TPT, Raoult D, Fournier PE, Fenollar F, Levasseur A.	New Microbes New Infect	10.1016/j.nmni.2020.100719	2020
Phylogeny	Clostridium beihaiense sp. nov., an anaerobic bacterium isolated from activated sludge.	Dong Y, Liu Y, Chen N, Zhong Y, Liu L, Xie Q	Int J Syst Evol Microbiol	10.1099/ijsem.0.002885	2018
Phylogeny	Clostridium liquoris sp. nov., isolated from a fermentation pit used for the production of Chinese strong-flavoured liquor.	Yin Q, Tao Y, Zhu X, Zhou Y, He X, Cheng L, Huang Y, Li D	Int J Syst Evol Microbiol	10.1099/ijsem.0.000787	2015
Phylogeny	Clostridium lundense sp. nov., a novel anaerobic lipolytic bacterium isolated from bovine rumen.	Cirne DG, Delgado OD, Marichamy S, Mattiasson B	Int J Syst Evol Microbiol	10.1099/ijs.0.63730-0	2006

References

#1687	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 4474
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Clostridium tetanomorphum (DSM 4474, ATCC 49273, NCTC 543)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Compound production

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_017873215

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

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