Strain identifier
BacDive ID: 2727
Type strain: 
Species: Clostridium tetanomorphum
Strain Designation: 259 E.III.
Strain history: <- NCTC <- M. Robertson, Lister Institute, London, U.K.; 259 E.III. {1920}
NCBI tax ID(s): 1553 (species)
version 9.3 (current version)
General
@ref: 1687
BacDive-ID: 2727
DSM-Number: 4474
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, alcohol production
description: Clostridium tetanomorphum 259 E.III. is an anaerobe, mesophilic bacterium that produces alcohol and was isolated from septic wound.
NCBI tax id
- NCBI tax id: 1553
- Matching level: species
strain history
- @ref: 1687
- history: <- NCTC <- M. Robertson, Lister Institute, London, U.K.; 259 E.III. {1920}
doi: 10.13145/bacdive2727.20250331.9.3
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Clostridium
- species: Clostridium tetanomorphum
- full scientific name: Clostridium tetanomorphum (ex Bulloch et al. 1919) Wilde et al. 1989
@ref: 1687
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Clostridiaceae
genus: Clostridium
species: Clostridium tetanomorphum
full scientific name: Clostridium tetanomorphum (ex Bulloch et al. 1919) Wilde et al. 1989
strain designation: 259 E.III.
type strain: yes
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 1687 | WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) | yes | https://mediadive.dsmz.de/medium/339 | Name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water |
| 1687 | CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) | yes | https://mediadive.dsmz.de/medium/110 | Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water |
culture temp
- @ref: 1687
- growth: positive
- type: growth
- temperature: 37
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 1687 | anaerobe | |
| 125438 | anaerobe | 90.105 |
| 125439 | anaerobe | 98.5 |
compound production
- @ref: 1687
- compound: n butanol
Isolation, sampling and environmental information
isolation
- @ref: 1687
- sample type: septic wound
- country: United Kingdom
- origin.country: GBR
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Infection | #Disease | |
| #Host Body-Site | #Other | #Wound |
taxonmaps
- @ref: 69479
- File name: preview.99_5107.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_34;96_2485;97_3035;98_3812;99_5107&stattab=map
- Last taxonomy: Clostridium tetanomorphum subclade
- 16S sequence: DQ241819
- Sequence Identity:
- Total samples: 8830
- soil counts: 1355
- aquatic counts: 850
- animal counts: 6449
- plant counts: 176
Safety information
risk assessment
- @ref: 1687
- pathogenicity human: yes, in single cases
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Clostridium tetanomorphum strain DSM 4474 16S ribosomal RNA gene, partial sequence | DQ911261 | 320 | nuccore | 1553 |
| 1687 | Clostridium tetanomorphum strain DSM 4474 16S ribosomal RNA gene, partial sequence | DQ241819 | 1407 | nuccore | 1553 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Clostridium tetanomorphum DSM 4474 | GCA_017873215 | contig | ncbi | 1553 |
| 66792 | Clostridium tetanomorphum strain DSM 4474 | 1553.15 | wgs | patric | 1553 |
| 66792 | Clostridium tetanomorphum DSM 4474 | 2913338184 | draft | img | 1553 |
Genome-based predictions
predictions
| @ref | model | trait | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 50.732 | no |
| 125438 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | yes | 90.105 | no |
| 125438 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 76.797 | no |
| 125438 | thermophile | thermophilic | Ability to grow at temperatures above or equal to 45°C | no | 88.663 | yes |
| 125438 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | no | 96.642 | yes |
| 125438 | motile2+ | flagellated | Ability to perform flagellated movement | yes | 75.259 | no |
| 125439 | BacteriaNet | spore_formation | Ability to form endo- or exospores | yes | 76.9 | |
| 125439 | BacteriaNet | motility | Ability to perform movement | yes | 76.2 | |
| 125439 | BacteriaNet | gram_stain | Reaction to gram-staining | positive | 76.8 | |
| 125439 | BacteriaNet | oxygen_tolerance | Oxygenic conditions needed for growth | anaerobe | 98.5 |
External links
@ref: 1687
culture collection no.: DSM 4474, ATCC 49273, NCTC 543
straininfo link
- @ref: 72255
- straininfo: 42396
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 16514039 | Clostridium lundense sp. nov., a novel anaerobic lipolytic bacterium isolated from bovine rumen. | Cirne DG, Delgado OD, Marichamy S, Mattiasson B | Int J Syst Evol Microbiol | 10.1099/ijs.0.63730-0 | 2006 | Animals, Cattle/*microbiology, Clostridium/*classification/genetics/isolation & purification/physiology, DNA, Ribosomal/chemistry, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Rumen/*microbiology, Sequence Analysis, DNA | Phenotype |
| Phylogeny | 26597830 | Clostridium liquoris sp. nov., isolated from a fermentation pit used for the production of Chinese strong-flavoured liquor. | Yin Q, Tao Y, Zhu X, Zhou Y, He X, Cheng L, Huang Y, Li D | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000787 | 2015 | Biotechnology | |
| Phylogeny | 30028283 | Clostridium beihaiense sp. nov., an anaerobic bacterium isolated from activated sludge. | Dong Y, Liu Y, Chen N, Zhong Y, Liu L, Xie Q | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002885 | 2018 | Bacterial Typing Techniques, Base Composition, China, Clostridium/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Waste Water/microbiology | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 1687 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4474) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-4474 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 72255 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID42396.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
| 125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann | 10.1101/2024.08.12.607695 | Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets | |||
| 125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy | https://github.com/GenomeNet/deepG | deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 |