Name: Actinopolyspora saharensis H32
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 24728

Type strain:

Species: Actinopolyspora saharensis

Strain Designation: H32

Culture col. no.: DSM 45459, CCUG 63367, MTCC 11557, JCM 30062

Strain history: MTCC 11557 <-- N. Sabaou H32.

NCBI tax ID(s): 995062 (species)

SI-ID 397729

DSM 45459, CCUG 63367

Special Collections

Other strains from Actinopolyspora saharensis

Section

Actinopolyspora saharensis H32 is an aerobe, spore-forming, mesophilic bacterium that was isolated from desert soil.

spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Actinopolyspora*
	Species	**Actinopolyspora saharensis**
	Full Scientific Name (LPSN)	Actinopolyspora saharensis Meklat et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %91.551

[Ref.: #69480]	Motility	no Confidence in %90.5

[Ref.: #62969]

Incubation period

18-20 days

Multimedia content

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Caption:

Medium 1159 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

CM + YE MEDIUM (DSMZ Medium 549)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

GYM STREPTOMYCES MEDIUM 10% NACL (DSMZ Medium 1159)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #20250]

Culture medium composition

expand / minimize

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Culture medium

NUTRIMENT AGAR WITH 150G/L NACL (DSMZ Medium 1258)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #20250]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #20250]	growth	28 °C
[Ref.: #62969]	growth	30 °C
[Ref.: #67770]	growth	28 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #62969]

Oxygen tolerance

aerobe

[Ref.: #69481]

Ability of spore formation

yes Confidence in %100

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Observation

quinones: MK-10(H₄), MK-9(H₄)

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20250]	Sample type/isolated from	desert soil
[Ref.: #20250]	Geographic location (country and/or sea, region)	Sahara, El-Oued province
[Ref.: #20250]	Country	Algeria
[Ref.: #20250]	Country ISO 3 Code	DZA
[Ref.: #20250]	Continent	Africa

[Ref.: #62969]	Sample type/isolated from	Soil,Sahara desert
[Ref.: #62969]	Sampling date	2006
[Ref.: #62969]	Geographic location (country and/or sea, region)	south,El-Oued province
[Ref.: #62969]	Country	Algeria
[Ref.: #62969]	Country ISO 3 Code	DZA
[Ref.: #62969]	Continent	Africa

[Ref.: #67770]	Sample type/isolated from	Saharan soil from El-Oued Province
[Ref.: #67770]	Country	Algeria
[Ref.: #67770]	Country ISO 3 Code	DZA
[Ref.: #67770]	Continent	Africa
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Desert
#Environmental	#Terrestrial	#Soil
#Condition	#Xerophilic	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HQ918198 (>99% sequence identity) for Actinopolyspora from Microbeatlas

Aquatic Samples	2
Soil Samples
Animal Samples	10
Plant Samples
Total Samples	12

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20250]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20250]	Actinopolyspora saharensis strain H32 16S ribosomal RNA gene, partial sequence	HQ918198	1523	GenBank ENA	995062 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Actinopolyspora saharensis DSM 45459	GCA_900100925	contig	GenBank ENA	995062 tax ID
[Ref.: #66792]	Actinopolyspora saharensis strain DSM 45459	995062.3	wgs	PATRIC	995062 tax ID	*
[Ref.: #66792]	Actinopolyspora saharensis DSM 45459	2634166628	draft	JGI IMG/M	995062 tax ID	*

[Ref.: #67770]

GC-content

69.5 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Actinopolyspora saharensis strain DSM 45459 PATRIC: 995062.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinopolyspora saharensis strain DSM 45459 PATRIC: 995062.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	91.551	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	94.682	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	88.767	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	81.57	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	93.322	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	90.5	no

Availability in culture collections External links

[Ref.: #20250]

Culture collection no.

DSM 45459, CCUG 63367, MTCC 11557, JCM 30062

[Ref.: #88556]

SI-ID 397729

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinopolyspora biskrensis sp. nov., a novel halophilic actinomycete isolated from Northern Sahara.	Saker R, Bouras N, Meklat A, Zitouni A, Schumann P, Sproer C, Klenk HP, Sabaou N	Curr Microbiol	10.1007/s00284-014-0740-3	2014	*
Phylogeny	Actinopolyspora saharensis sp. nov., a novel halophilic actinomycete isolated from a Saharan soil of Algeria.	Meklat A, Bouras N, Zitouni A, Mathieu F, Lebrihi A, Schumann P, Sproer C, Klenk HP, Sabaou N	Antonie Van Leeuwenhoek	10.1007/s10482-012-9859-z	2012	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20250

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45459

#62969 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 63367

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#88556

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID397729.1 )

* These data were automatically processed and therefore are not curated

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Actinopolyspora saharensis strain DSM 45459

Actinopolyspora saharensis strain DSM 45459

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