Actinopolyspora saharensis (DSM 45459, CCUG 63367, MTCC 11557)

Name: Actinopolyspora saharensis (DSM 45459, CCUG 63367, MTCC 11557)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 24728

Type strain

Strain Designation H32

Culture col. no. DSM 45459 CCUG 63367 MTCC 11557 H32 JCM 30062

NCBI tax ID(s) 995062

Special Collections DSMZ CCUG JCM

Links

Actinopolyspora saharensis H32 is an aerobe, mesophilic prokaryote that was isolated from desert soil.

aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 397729

DSM 45459,CCUG 63367

@ref 20215

Last LPSN update 2025-12-16 09:52:55

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Actinopolyspora

Species Actinopolyspora saharensis

Full scientific name Actinopolyspora saharensis Meklat et al. 2013

BacDive ID	Other strains from **Actinopolyspora saharensis** (1)	Type strain
160699	A. saharensis H133, DSM 46667

Morphology

Cell morphology

@ref	Confidence	Gram stain
125438	90.5
125438	91.551	positive
125439	96.9	positive

Colony morphology

62969

Incubation period18-20 days

Multimedia

@ref:	20250
multimedia content:	DSM_45459.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_45459.jpg
caption:	Medium 1159 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
20250	CM + YE MEDIUM (DSMZ Medium 549)	Medium recipe at MediaDive	Name: CM + YE MEDIUM (DSMZ Medium 549) Composition: NaCl 200.0 g/l MgSO4 x 7 H2O 20.0 g/l Agar 15.0 g/l Yeast extract 10.0 g/l Casamino acids 7.5 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l FeSO4 x 7 H2O 0.01 g/l HCl 3.646e-05 g/l Distilled water
20250	GYM STREPTOMYCES MEDIUM 10% NACL (DSMZ Medium 1159)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM 10% NACL (DSMZ Medium 1159) Composition: NaCl 100.0 g/l Agar 15.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20250	NUTRIMENT AGAR WITH 150G/L NACL (DSMZ Medium 1258)	Medium recipe at MediaDive	Name: NUTRIMENT AGAR WITH 150 G/L NACL (DSMZ Medium 1258) Composition: NaCl 150.0 g/l Agar 20.0 g/l Peptone 5.0 g/l Yeast extract 2.0 g/l Meat extract 1.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
20250	positive	growth	28	mesophilic
62969	positive	growth	30	mesophilic
67770	positive	growth	28	mesophilic

Physiology and metabolism

Oxygen tolerance

62969

Oxygen toleranceaerobe

Observation

67770

Observationquinones: MK-10(H₄), MK-9(H₄)

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Desert
#Environmental	#Terrestrial	#Soil
#Condition	#Xerophilic	-

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent
20250	desert soil		Sahara, El-Oued province	Algeria	DZA	Africa
62969	Soil,Sahara desert	2006	south,El-Oued province	Algeria	DZA	Africa
67770	Saharan soil from El-Oued Province			Algeria	DZA	Africa

Taxonmaps

69479

Global distribution of 16S sequence HQ918198 (>99% sequence identity) for Actinopolyspora from Microbeatlas

Aquatic Samples	2
Soil Samples
Animal Samples	10
Plant Samples
Total Samples	12

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
20250	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	IMG-taxon 2634166628 annotated assembly for Actinopolyspora saharensis DSM 45459	contig	GCA_900100925	995062.3	2634166628	995062	76.91

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20250	Actinopolyspora saharensis strain H32 16S ribosomal RNA gene, partial sequence	HQ918198	1523		995062

GC content

@ref	GC-content (mol%)	Method
67770	69.5	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Actinopolyspora saharensis DSM 45459
NCBI: GCA_900100925

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	58.40	no
125439	motility	BacteriaNetⓘ	no	78.50	no
125439	gram_stain	BacteriaNetⓘ	positive	96.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	73.90	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinopolyspora saharensis strain DSM 45459
PATRIC: 995062.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	91.55	no
125438	anaerobic	anaerobicⓘ	no	94.68	no
125438	aerobic	aerobicⓘ	yes	88.77	no
125438	spore-forming	spore-formingⓘ	yes	81.57	no
125438	thermophilic	thermophileⓘ	no	93.32	yes
125438	flagellated	motile2+ⓘ	no	90.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Whole-genome sequencing of two Streptomyces strains isolated from the sand dunes of Sahara.	Zerouki C, Bensalah F, Kuittinen S, Pappinen A, Turunen O.	BMC Genomics	10.1186/s12864-021-07866-x	2021
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018
Phylogeny	Actinopolyspora biskrensis sp. nov., a novel halophilic actinomycete isolated from Northern Sahara.	Saker R, Bouras N, Meklat A, Zitouni A, Schumann P, Sproer C, Klenk HP, Sabaou N	Curr Microbiol	10.1007/s00284-014-0740-3	2014
Phylogeny	Actinopolyspora saharensis sp. nov., a novel halophilic actinomycete isolated from a Saharan soil of Algeria.	Meklat A, Bouras N, Zitouni A, Mathieu F, Lebrihi A, Schumann P, Sproer C, Klenk HP, Sabaou N	Antonie Van Leeuwenhoek	10.1007/s10482-012-9859-z	2012

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20250	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45459
#62969	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 63367
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinopolyspora saharensis (DSM 45459, CCUG 63367, MTCC 11557)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Observation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900100925

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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