Strain identifier

BacDive ID: 24728

Type strain: Yes

Species: Actinopolyspora saharensis

Strain Designation: H32

Strain history: MTCC 11557 <-- N. Sabaou H32.

NCBI tax ID(s): 995062 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 20250

BacDive-ID: 24728

DSM-Number: 45459

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic

description: Actinopolyspora saharensis H32 is an aerobe, spore-forming, mesophilic bacterium that was isolated from desert soil.

NCBI tax id

  • NCBI tax id: 995062
  • Matching level: species

strain history

@refhistory
20250<- N. Sabaou, LPBVB, Kouba, Algeria; H32
67770MTCC 11557 <-- N. Sabaou H32.

doi: 10.13145/bacdive24728.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Actinopolyspora
  • species: Actinopolyspora saharensis
  • full scientific name: Actinopolyspora saharensis Meklat et al. 2013

@ref: 20250

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinopolysporaceae

genus: Actinopolyspora

species: Actinopolyspora saharensis

full scientific name: Actinopolyspora saharensis Meklat et al. 2013 emend. Nouioui et al. 2018

strain designation: H32

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no94.308
69480100positive

colony morphology

  • @ref: 62969
  • incubation period: 18-20 days

multimedia

  • @ref: 20250
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45459.jpg
  • caption: Medium 1159 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
20250CM + YE MEDIUM (DSMZ Medium 549)yeshttps://mediadive.dsmz.de/medium/549Name: CM + YE MEDIUM (DSMZ Medium 549) Composition: NaCl 200.0 g/l MgSO4 x 7 H2O 20.0 g/l Agar 15.0 g/l Yeast extract 10.0 g/l Casamino acids 7.5 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l FeSO4 x 7 H2O 0.01 g/l HCl 3.646e-05 g/l Distilled water
20250GYM STREPTOMYCES MEDIUM 10% NACL (DSMZ Medium 1159)yeshttps://mediadive.dsmz.de/medium/1159Name: GYM STREPTOMYCES MEDIUM 10% NACL (DSMZ Medium 1159) Composition: NaCl 100.0 g/l Agar 15.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20250NUTRIMENT AGAR WITH 150G/L NACL (DSMZ Medium 1258)yeshttps://mediadive.dsmz.de/medium/1258Name: NUTRIMENT AGAR WITH 150 G/L NACL (DSMZ Medium 1258) Composition: NaCl 150.0 g/l Agar 20.0 g/l Peptone 5.0 g/l Yeast extract 2.0 g/l Meat extract 1.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
20250positivegrowth28mesophilic
62969positivegrowth30mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 62969
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481yes100
69480yes100

observation

  • @ref: 67770
  • observation: quinones: MK-10(H4), MK-9(H4)

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
20250desert soilSahara, El-Oued provinceAlgeriaDZAAfrica
62969Soil,Sahara desertsouth,El-Oued provinceAlgeriaDZAAfrica2006
67770Saharan soil from El-Oued ProvinceAlgeriaDZAAfrica

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Desert
#Environmental#Terrestrial#Soil
#Condition#Xerophilic

taxonmaps

  • @ref: 69479
  • File name: preview.99_50126.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_495;96_2401;97_2923;98_3701;99_50126&stattab=map
  • Last taxonomy: Actinopolyspora
  • 16S sequence: HQ918198
  • Sequence Identity:
  • Total samples: 12
  • aquatic counts: 2
  • animal counts: 10

Safety information

risk assessment

  • @ref: 20250
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20250
  • description: Actinopolyspora saharensis strain H32 16S ribosomal RNA gene, partial sequence
  • accession: HQ918198
  • length: 1523
  • database: ena
  • NCBI tax ID: 995062

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinopolyspora saharensis strain DSM 45459995062.3wgspatric995062
66792Actinopolyspora saharensis DSM 454592634166628draftimg995062
67770Actinopolyspora saharensis DSM 45459GCA_900100925contigncbi995062

GC content

  • @ref: 67770
  • GC-content: 69.5
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno91.417no
flagellatedno97.858no
gram-positiveyes90.401no
anaerobicno98.591yes
halophileyes76.464no
spore-formingyes76.872no
thermophileno92.371no
glucose-utilyes86.759no
aerobicyes88.554yes
glucose-fermentno87.848no

External links

@ref: 20250

culture collection no.: DSM 45459, CCUG 63367, MTCC 11557, JCM 30062

straininfo link

  • @ref: 88556
  • straininfo: 397729

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny23196893Actinopolyspora saharensis sp. nov., a novel halophilic actinomycete isolated from a Saharan soil of Algeria.Meklat A, Bouras N, Zitouni A, Mathieu F, Lebrihi A, Schumann P, Sproer C, Klenk HP, Sabaou NAntonie Van Leeuwenhoek10.1007/s10482-012-9859-z2012Actinomycetales/*classification/growth & development/*isolation & purification, Algeria, Bacterial Typing Techniques, Carbohydrates/analysis, Cell Wall/chemistry, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, *Soil Microbiology, Temperature, Vitamin K 2/analysisGenetics
Phylogeny25429761Actinopolyspora biskrensis sp. nov., a novel halophilic actinomycete isolated from Northern Sahara.Saker R, Bouras N, Meklat A, Zitouni A, Schumann P, Sproer C, Klenk HP, Sabaou NCurr Microbiol10.1007/s00284-014-0740-32014Actinomycetaceae/*classification/genetics/isolation & purification/metabolism, Africa, Northern, Metabolomics, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAPhenotype

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
20250Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-45459Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45459)
62969Curators of the CCUGhttps://www.ccug.se/strain?id=63367Culture Collection University of Gothenburg (CCUG) (CCUG 63367)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
88556Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397729.1