Strain identifier
BacDive ID: 24728
Type strain:
Species: Actinopolyspora saharensis
Strain Designation: H32
Strain history: MTCC 11557 <-- N. Sabaou H32.
NCBI tax ID(s): 995062 (species)
General
@ref: 20250
BacDive-ID: 24728
DSM-Number: 45459
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic
description: Actinopolyspora saharensis H32 is an aerobe, spore-forming, mesophilic bacterium that was isolated from desert soil.
NCBI tax id
- NCBI tax id: 995062
- Matching level: species
strain history
@ref | history |
---|---|
20250 | <- N. Sabaou, LPBVB, Kouba, Algeria; H32 |
67770 | MTCC 11557 <-- N. Sabaou H32. |
doi: 10.13145/bacdive24728.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Pseudonocardiales
- family: Pseudonocardiaceae
- genus: Actinopolyspora
- species: Actinopolyspora saharensis
- full scientific name: Actinopolyspora saharensis Meklat et al. 2013
@ref: 20250
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Actinopolysporaceae
genus: Actinopolyspora
species: Actinopolyspora saharensis
full scientific name: Actinopolyspora saharensis Meklat et al. 2013 emend. Nouioui et al. 2018
strain designation: H32
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain |
---|---|---|---|
69480 | no | 94.308 | |
69480 | 100 | positive |
colony morphology
- @ref: 62969
- incubation period: 18-20 days
multimedia
- @ref: 20250
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45459.jpg
- caption: Medium 1159 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
20250 | CM + YE MEDIUM (DSMZ Medium 549) | yes | https://mediadive.dsmz.de/medium/549 | Name: CM + YE MEDIUM (DSMZ Medium 549) Composition: NaCl 200.0 g/l MgSO4 x 7 H2O 20.0 g/l Agar 15.0 g/l Yeast extract 10.0 g/l Casamino acids 7.5 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l FeSO4 x 7 H2O 0.01 g/l HCl 3.646e-05 g/l Distilled water |
20250 | GYM STREPTOMYCES MEDIUM 10% NACL (DSMZ Medium 1159) | yes | https://mediadive.dsmz.de/medium/1159 | Name: GYM STREPTOMYCES MEDIUM 10% NACL (DSMZ Medium 1159) Composition: NaCl 100.0 g/l Agar 15.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
20250 | NUTRIMENT AGAR WITH 150G/L NACL (DSMZ Medium 1258) | yes | https://mediadive.dsmz.de/medium/1258 | Name: NUTRIMENT AGAR WITH 150 G/L NACL (DSMZ Medium 1258) Composition: NaCl 150.0 g/l Agar 20.0 g/l Peptone 5.0 g/l Yeast extract 2.0 g/l Meat extract 1.0 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
20250 | positive | growth | 28 | mesophilic |
62969 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 62969
- oxygen tolerance: aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | yes | 100 |
69480 | yes | 100 |
observation
- @ref: 67770
- observation: quinones: MK-10(H4), MK-9(H4)
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | sampling date |
---|---|---|---|---|---|---|
20250 | desert soil | Sahara, El-Oued province | Algeria | DZA | Africa | |
62969 | Soil,Sahara desert | south,El-Oued province | Algeria | DZA | Africa | 2006 |
67770 | Saharan soil from El-Oued Province | Algeria | DZA | Africa |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Desert |
#Environmental | #Terrestrial | #Soil |
#Condition | #Xerophilic |
taxonmaps
- @ref: 69479
- File name: preview.99_50126.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_495;96_2401;97_2923;98_3701;99_50126&stattab=map
- Last taxonomy: Actinopolyspora
- 16S sequence: HQ918198
- Sequence Identity:
- Total samples: 12
- aquatic counts: 2
- animal counts: 10
Safety information
risk assessment
- @ref: 20250
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 20250
- description: Actinopolyspora saharensis strain H32 16S ribosomal RNA gene, partial sequence
- accession: HQ918198
- length: 1523
- database: ena
- NCBI tax ID: 995062
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Actinopolyspora saharensis strain DSM 45459 | 995062.3 | wgs | patric | 995062 |
66792 | Actinopolyspora saharensis DSM 45459 | 2634166628 | draft | img | 995062 |
67770 | Actinopolyspora saharensis DSM 45459 | GCA_900100925 | contig | ncbi | 995062 |
GC content
- @ref: 67770
- GC-content: 69.5
- method: genome sequence analysis
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
motile | no | 91.417 | no |
flagellated | no | 97.858 | no |
gram-positive | yes | 90.401 | no |
anaerobic | no | 98.591 | yes |
halophile | yes | 76.464 | no |
spore-forming | yes | 76.872 | no |
thermophile | no | 92.371 | no |
glucose-util | yes | 86.759 | no |
aerobic | yes | 88.554 | yes |
glucose-ferment | no | 87.848 | no |
External links
@ref: 20250
culture collection no.: DSM 45459, CCUG 63367, MTCC 11557, JCM 30062
straininfo link
- @ref: 88556
- straininfo: 397729
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 23196893 | Actinopolyspora saharensis sp. nov., a novel halophilic actinomycete isolated from a Saharan soil of Algeria. | Meklat A, Bouras N, Zitouni A, Mathieu F, Lebrihi A, Schumann P, Sproer C, Klenk HP, Sabaou N | Antonie Van Leeuwenhoek | 10.1007/s10482-012-9859-z | 2012 | Actinomycetales/*classification/growth & development/*isolation & purification, Algeria, Bacterial Typing Techniques, Carbohydrates/analysis, Cell Wall/chemistry, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, *Soil Microbiology, Temperature, Vitamin K 2/analysis | Genetics |
Phylogeny | 25429761 | Actinopolyspora biskrensis sp. nov., a novel halophilic actinomycete isolated from Northern Sahara. | Saker R, Bouras N, Meklat A, Zitouni A, Schumann P, Sproer C, Klenk HP, Sabaou N | Curr Microbiol | 10.1007/s00284-014-0740-3 | 2014 | Actinomycetaceae/*classification/genetics/isolation & purification/metabolism, Africa, Northern, Metabolomics, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Phenotype |
Reference
@id | authors | title | doi/url | catalogue |
---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
20250 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-45459 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45459) | |
62969 | Curators of the CCUG | https://www.ccug.se/strain?id=63367 | Culture Collection University of Gothenburg (CCUG) (CCUG 63367) | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
88556 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID397729.1 |