Neobacillus ginsengisoli (DSM 27594, JCM 17335, KCTC 13945)

Name: Neobacillus ginsengisoli (DSM 27594, JCM 17335, KCTC 13945)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 23709

Type strain

Culture col. no. DSM 27594 JCM 17335 KCTC 13945 DCY 53

NCBI tax ID(s) 904295

Special Collections DSMZ JCM KCTC Literature strains

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Neobacillus ginsengisoli DSM 27594 is an aerobe, spore-forming, mesophilic prokaryote that was isolated from ginseng soil.

spore-forming Gram-positive motile rod-shaped aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 407750

KCTC 13945,JCM 17335,DSM 27594

@ref 20215

Last LPSN update 2025-12-16 09:52:42

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Cytobacillaceae

Genus Neobacillus

Species Neobacillus ginsengisoli

Full scientific name Neobacillus ginsengisoli (Nguyen et al. 2013) Gupta et al. 2020

Synonyms (1)

Bacillus ginsengisoli

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Flagellum arrangement
30250	positive	6.5 µm	1.2 µm	rod-shaped
67771				rod-shaped	lophotrichous
67771	positive

Pigmentation

30250

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
20437	R2A MEDIUM (DSMZ Medium 830)		Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
20437	positive	growth	30	mesophilic
30250	positive	growth	15-45
30250	positive	optimum	33.5	mesophilic
67770	positive	growth	30	mesophilic
67771	positive	growth	30-37	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
30250	positive	optimum	6.75
30250	positive	growth	4.5-8.5	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
30250	aerobe
67771	aerobe
125439	obligate aerobe	94.2

Spore formation

@ref	Type of spore	Confidence
30250
67771	endospore
125439		90

Halophily

@ref	Salt	Growth	Tested relation	Concentration
30250	NaCl	positive	growth	0-2 %

Observation

@ref	Observation
67770	quinones: MK-7
67771	quinones: MK-7

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
30250	16193 ChEBI	3-hydroxybenzoate	+	carbon source
30250	37054 ChEBI	3-hydroxybutyrate	+	carbon source
30250	16449 ChEBI	alanine	+	carbon source
30250	4853 ChEBI	esculin	+	hydrolysis
30250	17234 ChEBI	glucose	+	carbon source
30250	28087 ChEBI	glycogen	+	carbon source
30250	24996 ChEBI	lactate	+	carbon source
30250	17306 ChEBI	maltose	+	carbon source
30250	29864 ChEBI	mannitol	+	carbon source
30250	37684 ChEBI	mannose	+	carbon source
30250	506227 ChEBI	N-acetylglucosamine	+	carbon source
30250	17632 ChEBI	nitrate	+	reduction
30250	26271 ChEBI	proline	+	carbon source
30250	17992 ChEBI	sucrose	+	carbon source

Enzymes

@ref	Value	Activity	Ec
30250	acid phosphatase	+	3.1.3.2
30250	alkaline phosphatase	+	3.1.3.1
30250	alpha-galactosidase	+	3.2.1.22
30250	catalase	+	1.11.1.6
30250	cytochrome oxidase	+	1.9.3.1

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Herbaceous plants (Grass,Crops)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
20437	ginseng soil	Pocheon Province	Republic of Korea	KOR	Asia
67770	Ginseng soil		Republic of Korea	KOR	Asia
67771	From 6-year-old ginseng soil	Pocheon	Republic of Korea	KOR	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
20437	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM3081305v1 assembly for Neobacillus ginsengisoli DSM 27594	contig	GCA_030813055	904295.3	2926503875	904295	63.08

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20437	Bacillus ginsengisoli strain DCY53 16S ribosomal RNA gene, partial sequence	HQ224517	1437		904295

GC content

@ref	GC-content (mol%)	Method
20437	43.6
67770	43.6	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Bacillus ginsengisoli DSM 27594
IMG: 2926503875

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	90.00	no
125439	motility	BacteriaNetⓘ	yes	80.20	no
125439	gram_stain	BacteriaNetⓘ	variable	81.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	94.20	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Neobacillus driksii sp. nov. isolated from a Mars 2020 spacecraft assembly facility and genomic potential for lasso peptide production in Neobacillus.	Hameed A, McDonagh F, Sengupta P, Miliotis G, Sivabalan SKM, Szydlowski L, Simpson A, Singh NK, Rekha PD, Raman K, Venkateswaran K.	Microbiol Spectr	10.1128/spectrum.01376-24	2025
Phylogeny	Bacillus ginsengisoli sp. nov., isolated from soil of a ginseng field.	Nguyen NL, Kim YJ, Hoang VA, Min JW, Liang ZQ, Yang DC	Int J Syst Evol Microbiol	10.1099/ijs.0.031740-0	2012

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20437	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 27594
#30250	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26592 (see below)
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Neobacillus ginsengisoli (DSM 27594, JCM 17335, KCTC 13945)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_030813055

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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