Name: Neobacillus ginsengisoli DSM 27594
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 23709

Type strain:

Species: Neobacillus ginsengisoli

Culture col. no.: DSM 27594, JCM 17335, KCTC 13945, DCY 53

Strain history: <- YJ Kim, Kyunghee Univ.

NCBI tax ID(s): 904295 (species)

SI-ID 407750

DSM 27594

Special Collections

Section

Neobacillus ginsengisoli DSM 27594 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from ginseng soil.

Gram-positive
motile
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Neobacillus*
	Species	**Neobacillus ginsengisoli**
	Full Scientific Name (LPSN)	Neobacillus ginsengisoli (Nguyen et al. 2013) Gupta et al. 2020

Synonym

Bacillus ginsengisoli

Information on morphological and physiological properties Morphology

[Ref.: #30250]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #30250]	Cell length	6.5 µm

[Ref.: #30250]	Cell width	1.2 µm

[Ref.: #30250]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #30250]	Motility	yes
[Ref.: #67771]	Motility	yes

[Ref.: #67771]	Flagellum arrangement	lophotrichous

[Ref.: #30250]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #20437]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #20437]

Culture medium growth

[Ref.: #20437]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20437]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #20437]	growth	30 °C
[Ref.: #30250]	growth	15-45 °C
[Ref.: #30250]	optimum	33.5 °C
[Ref.: #67770]	growth	30 °C
[Ref.: #67771]	growth	30-37 °C

	pH	Kind of pH		pH
[Ref.: #30250]			optimum	6.75
[Ref.: #30250]			growth	4.5-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30250]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #67771]	Type of spore	endospore
[Ref.: #67771]	Ability of spore formation	yes
[Ref.: #30250]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30250]		NaCl		growth	0-2	%

[Ref.: #67770]	Observation	quinones: MK-7
[Ref.: #67771]	Observation	quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30250]	3-hydroxybenzoate ChEBI	+	carbon source
[Ref.: #30250]	3-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #30250]	alanine ChEBI	+	carbon source
[Ref.: #30250]	esculin ChEBI	+	hydrolysis
[Ref.: #30250]	glucose ChEBI	+	carbon source
[Ref.: #30250]	glycogen ChEBI	+	carbon source
[Ref.: #30250]	lactate ChEBI	+	carbon source
[Ref.: #30250]	maltose ChEBI	+	carbon source
[Ref.: #30250]	mannitol ChEBI	+	carbon source
[Ref.: #30250]	mannose ChEBI	+	carbon source
[Ref.: #30250]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #30250]	nitrate ChEBI	+	reduction
[Ref.: #30250]	proline ChEBI	+	carbon source
[Ref.: #30250]	sucrose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #30250]	acid phosphatase	+	3.1.3.2
[Ref.: #30250]	alkaline phosphatase	+	3.1.3.1
[Ref.: #30250]	alpha-galactosidase	+	3.2.1.22
[Ref.: #30250]	catalase	+	1.11.1.6
[Ref.: #30250]	cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20437]	Sample type/isolated from	ginseng soil
[Ref.: #20437]	Geographic location (country and/or sea, region)	Pocheon Province
[Ref.: #20437]	Country	Republic of Korea
[Ref.: #20437]	Country ISO 3 Code	KOR
[Ref.: #20437]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Ginseng soil
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From 6-year-old ginseng soil
[Ref.: #67771]	Geographic location (country and/or sea, region)	Pocheon
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20437]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20437]	Bacillus ginsengisoli strain DCY53 16S ribosomal RNA gene, partial sequence	HQ224517	1437	GenBank ENA	904295 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus ginsengisoli DSM 27594	2926503875	draft	JGI IMG/M	904295 tax ID	*

[Ref.: #20437]	GC-content	43.6 mol%
[Ref.: #67770]	GC-content	43.6 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #20437]

Culture collection no.

DSM 27594, JCM 17335, KCTC 13945, DCY 53

[Ref.: #87755]

SI-ID 407750

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus ginsengisoli sp. nov., isolated from soil of a ginseng field.	Nguyen NL, Kim YJ, Hoang VA, Min JW, Liang ZQ, Yang DC	Int J Syst Evol Microbiol	10.1099/ijs.0.031740-0	2012	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20437

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 27594

#30250

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26592 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#87755

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID407750.1 )

#26592

IJSEM 855 2013 ( DOI 10.1099/ijs.0.031740-0 , PubMed 22611202 )

* These data were automatically processed and therefore are not curated

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