Oligosphaera ethanolica (DSM 24202, JCM 17152, 8KG-4)

Name: Oligosphaera ethanolica (DSM 24202, JCM 17152, 8KG-4)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 23458

Type strain

Strain Designation 8KG-4

Culture col. no. DSM 24202 JCM 17152 8KG-4 KCTC 15378 CGMCC 1.5160

NCBI tax ID(s) 760260

Special Collections DSMZ JCM KCTC Literature strains

History <- Q. Yan-ling, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences (CAS), Qingdao; 8KG-4 <- Q. Yan-ling {2010} Y.-L. Qiu and Y. Sekiguchi 8KG-4.

Links

Oligosphaera ethanolica 8KG-4 is an anaerobe, mesophilic, Gram-negative prokaryote that was isolated from methanogenic, mesophilic granular sludge from a reactor treating wastewater from salted vegetable production processes.

Gram-negative coccus-shaped anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 407412

JCM 17152,DSM 24202

@ref 20215

Last LPSN update 2025-12-16 09:52:40

Domain Pseudomonadati

Phylum Lentisphaerota

Class Oligosphaeria

Order Oligosphaerales

Family Oligosphaeraceae

Genus Oligosphaera

Species Oligosphaera ethanolica

Full scientific name Oligosphaera ethanolica Qiu et al. 2013

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
30595	negative	1 µm	1 µm	coccus-shaped

Colony morphology

19258

Incubation period8-14 days

Pigmentation

30595

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
19258	PELOTOMACULUM MEDIUM (DSMZ Medium 960)		Medium recipe at MediaDive	Name: PELOTOMACULUM MEDIUM (DSMZ Medium 960; with strain-specific modifications) Composition: Na2CO3 1.49701 g/l NH4Cl 0.538922 g/l Na2S x 9 H2O 0.299401 g/l L-Cysteine HCl x H2O 0.299401 g/l MgCl2 x 6 H2O 0.199601 g/l D-Glucose 0.179641 g/l KH2PO4 0.139721 g/l Nitrilotriacetic acid 0.0127745 g/l FeCl2 x 4 H2O 0.000998004 g/l NaCl 0.000998004 g/l Sodium resazurin 0.000499002 g/l ZnCl2 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l NiCl2 x 6 H2O 9.98004e-05 g/l CaCl2 x 2 H2O 9.98004e-05 g/l Pyridoxine hydrochloride 9.98004e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l Na2WO4 x 2 H2O 3.99202e-05 g/l CoCl2 x 6 H2O 2.99401e-05 g/l Na2MoO4 x 2 H2O 2.99401e-05 g/l Na2SeO3 x 5 H2O 2.99401e-05 g/l CuCl2 1.99601e-05 g/l Biotin 1.99601e-05 g/l Folic acid 1.99601e-05 g/l H3BO3 9.98004e-06 g/l Vitamin B12 9.98004e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
19258	positive	growth	37	mesophilic
30595	positive	growth	25-40
30595	positive	optimum	37	mesophilic
67770	positive	growth	37	mesophilic

Culture pH

@ref	Ability	Type	PH
30595	positive	optimum	7
30595	positive	growth	6.5-7.5

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
19258	anaerobe
30595	anaerobe

Spore formation

30595

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
30595	NaCl	positive	growth	0-1 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
30595	28260 ChEBI	galactose	+	carbon source
30595	17234 ChEBI	glucose	+	carbon source
30595	33942 ChEBI	ribose	+	carbon source
30595	17992 ChEBI	sucrose	+	carbon source
30595	18222 ChEBI	xylose	+	carbon source

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	-
#Engineered	#Waste	#Activated sludge
#Condition	#Anoxic (anaerobic)	-
#Condition	#Saline	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
19258	methanogenic, mesophilic granular sludge from a reactor treating wastewater from salted vegetable production processes	Tokyo	Japan	JPN	Asia
67770	Upflow anaerobic sludge blanket reactor treating high-strength organic wastewater from salted vegetable production processes

Taxonmaps

69479

Global distribution of 16S sequence AB558581 (>99% sequence identity) for Oligosphaera ethanolica subclade from Microbeatlas

Aquatic Samples	390
Soil Samples	84
Animal Samples	40
Plant Samples	69
Total Samples	583

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
19258	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM3081469v1 assembly for Oligosphaera ethanolica DSM 24202	contig	GCA_030814695	760260.3	2927555367	760260	77.01

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
19258	Oligosphaera ethanolica gene for 16S rRNA, partial sequence	AB558581	1434		760260

GC content

@ref	GC-content (mol%)	Method
19258	61.1	high performance liquid chromatography (HPLC)
30595	61.1

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Oligosphaera ethanolica gen. nov., sp. nov., an anaerobic, carbohydrate-fermenting bacterium isolated from methanogenic sludge, and description of Oligosphaeria classis nov. in the phylum Lentisphaerae.	Qiu YL, Muramatsu M, Hanada S, Kamagata Y, Guo RB, Sekiguchi Y	Int J Syst Evol Microbiol	10.1099/ijs.0.039545-0	2012

References

#19258	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24202
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#26926	IJSEM 533 2013 ( DOI 10.1099/ijs.0.039545-0 , PubMed 22523166 )
#30595	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26926
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Oligosphaera ethanolica (DSM 24202, JCM 17152, 8KG-4)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_030814695

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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