Name: Fontimonas thermophila HA-01
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 23456

Type strain:

Species: Fontimonas thermophila

Strain Designation: HA-01

Culture col. no.: DSM 23609, CCUG 59713

Strain history: <- P. Lawson, Univ. Oklahoma, USA; HA-01 <- N.A. Losey

NCBI tax ID(s): 1076937 (species)

SI-ID 405672

DSM 23609, CCUG 59713

Section

Fontimonas thermophila HA-01 is an aerobe, thermophilic, Gram-negative bacterium that was isolated from freshwater hot spring.

Gram-negative
motile
rod-shaped
aerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Nevskiales*
	Family	*Nevskiaceae*
	Genus	*Fontimonas*
	Species	**Fontimonas thermophila**
	Full Scientific Name (LPSN)	Fontimonas thermophila Losey et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30484]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.99

[Ref.: #30484]	Cell shape	rod-shaped

[Ref.: #30484]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %91.087

[Ref.: #30484]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #19194]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #19194]

Culture medium growth

[Ref.: #19194]

Culture medium link

Medium recipe at MediaDive

[Ref.: #19194]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #19194]	growth	50 °C
[Ref.: #30484]	growth	37-60 °C
[Ref.: #30484]	optimum	47.5 °C
[Ref.: #62085]	growth	56 °C

[Ref.: #19194]	Temperature range	thermophilic
[Ref.: #30484]	Temperature range	thermophilic
[Ref.: #62085]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #30484]			optimum	6.75
[Ref.: #30484]			growth	6.5-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30484]	Oxygen tolerance	aerobe
[Ref.: #62085]	Oxygen tolerance	aerobe

[Ref.: #30484]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30484]		NaCl		growth	0-1	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30484]	2-oxopentanoate ChEBI	+	carbon source
[Ref.: #30484]	alanine ChEBI	+	carbon source
[Ref.: #30484]	aspartate ChEBI	+	carbon source
[Ref.: #30484]	glutamate ChEBI	+	carbon source
[Ref.: #30484]	glycine ChEBI	+	carbon source
[Ref.: #30484]	L-alaninamide ChEBI	+	carbon source
[Ref.: #30484]	leucine ChEBI	+	carbon source
[Ref.: #30484]	methyl pyruvate ChEBI	+	carbon source
[Ref.: #30484]	proline ChEBI	+	carbon source
[Ref.: #30484]	tween 40 ChEBI	+	carbon source
[Ref.: #30484]	tween 80 ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #30484]	alkaline phosphatase	+	3.1.3.1
[Ref.: #30484]	catalase	+	1.11.1.6
[Ref.: #30484]	cytochrome oxidase	+	1.9.3.1

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	L-lactaldehyde degradation	100	3 of 3
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	reductive acetyl coenzyme A pathway	100	7 of 7
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	octane oxidation	100	3 of 3
[Ref.: #66794]	biotin biosynthesis	100	4 of 4
[Ref.: #66794]	sulfopterin metabolism	100	4 of 4
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	methylglyoxal degradation	100	5 of 5
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	tetrahydrofolate metabolism	92.86	13 of 14
[Ref.: #66794]	palmitate biosynthesis	90.91	20 of 22
[Ref.: #66794]	lipid A biosynthesis	88.89	8 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	molybdenum cofactor biosynthesis	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	valine metabolism	88.89	8 of 9
[Ref.: #66794]	gluconeogenesis	87.5	7 of 8
[Ref.: #66794]	NAD metabolism	83.33	15 of 18
[Ref.: #66794]	phenylacetate degradation (aerobic)	80	4 of 5
[Ref.: #66794]	propionate fermentation	80	8 of 10
[Ref.: #66794]	glutamate and glutamine metabolism	78.57	22 of 28
[Ref.: #66794]	citric acid cycle	78.57	11 of 14
[Ref.: #66794]	heme metabolism	78.57	11 of 14
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	CO2 fixation in Crenarchaeota	77.78	7 of 9
[Ref.: #66794]	leucine metabolism	76.92	10 of 13
[Ref.: #66794]	phenylalanine metabolism	76.92	10 of 13
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	C4 and CAM-carbon fixation	75	6 of 8
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
[Ref.: #66794]	isoleucine metabolism	75	6 of 8
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	methionine metabolism	73.08	19 of 26
[Ref.: #66794]	propanol degradation	71.43	5 of 7
[Ref.: #66794]	glycolysis	70.59	12 of 17
[Ref.: #66794]	vitamin B1 metabolism	69.23	9 of 13
[Ref.: #66794]	purine metabolism	69.15	65 of 94
[Ref.: #66794]	alanine metabolism	68.97	20 of 29
[Ref.: #66794]	androgen and estrogen metabolism	68.75	11 of 16
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	d-mannose degradation	66.67	6 of 9
[Ref.: #66794]	methane metabolism	66.67	2 of 3
[Ref.: #66794]	serine metabolism	66.67	6 of 9
[Ref.: #66794]	peptidoglycan biosynthesis	66.67	10 of 15
[Ref.: #66794]	lipid metabolism	64.52	20 of 31
[Ref.: #66794]	proline metabolism	63.64	7 of 11
[Ref.: #66794]	vitamin B6 metabolism	63.64	7 of 11
[Ref.: #66794]	tryptophan metabolism	60.53	23 of 38
[Ref.: #66794]	threonine metabolism	60	6 of 10
[Ref.: #66794]	flavin biosynthesis	60	9 of 15
[Ref.: #66794]	cellulose degradation	60	3 of 5
[Ref.: #66794]	lipoate biosynthesis	60	3 of 5
[Ref.: #66794]	pyrimidine metabolism	60	27 of 45
[Ref.: #66794]	arachidonate biosynthesis	60	3 of 5
[Ref.: #66794]	non-pathway related	57.89	22 of 38
[Ref.: #66794]	tyrosine metabolism	57.14	8 of 14
[Ref.: #66794]	ubiquinone biosynthesis	57.14	4 of 7
[Ref.: #66794]	glutathione metabolism	57.14	8 of 14
[Ref.: #66794]	cysteine metabolism	55.56	10 of 18
[Ref.: #66794]	histidine metabolism	55.17	16 of 29
[Ref.: #66794]	arginine metabolism	54.17	13 of 24
[Ref.: #66794]	isoprenoid biosynthesis	53.85	14 of 26
[Ref.: #66794]	3-phenylpropionate degradation	53.33	8 of 15
[Ref.: #66794]	lysine metabolism	52.38	22 of 42
[Ref.: #66794]	dTDPLrhamnose biosynthesis	50	4 of 8
[Ref.: #66794]	phenylmercury acetate degradation	50	1 of 2
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	toluene degradation	50	2 of 4
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	50	6 of 12
[Ref.: #66794]	glycogen biosynthesis	50	2 of 4
[Ref.: #66794]	cyclohexanol degradation	50	2 of 4
[Ref.: #66794]	butanoate fermentation	50	2 of 4
[Ref.: #66794]	oxidative phosphorylation	48.35	44 of 91
[Ref.: #66794]	sulfate reduction	46.15	6 of 13
[Ref.: #66794]	metabolism of disaccharids	45.45	5 of 11
[Ref.: #66794]	nitrate assimilation	44.44	4 of 9
[Ref.: #66794]	degradation of sugar alcohols	43.75	7 of 16
[Ref.: #66794]	cardiolipin biosynthesis	42.86	3 of 7
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	Entner Doudoroff pathway	40	4 of 10
[Ref.: #66794]	4-hydroxyphenylacetate degradation	40	4 of 10
[Ref.: #66794]	vitamin K metabolism	40	2 of 5
[Ref.: #66794]	D-cycloserine biosynthesis	40	2 of 5
[Ref.: #66794]	glycine betaine biosynthesis	40	2 of 5
[Ref.: #66794]	degradation of hexoses	38.89	7 of 18
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	38.46	5 of 13
[Ref.: #66794]	ketogluconate metabolism	37.5	3 of 8
[Ref.: #66794]	carnitine metabolism	37.5	3 of 8
[Ref.: #66794]	ascorbate metabolism	36.36	8 of 22
[Ref.: #66794]	cholesterol biosynthesis	36.36	4 of 11
[Ref.: #66794]	pentose phosphate pathway	36.36	4 of 11
[Ref.: #66794]	bile acid biosynthesis, neutral pathway	35.29	6 of 17
[Ref.: #66794]	phenol degradation	35	7 of 20
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	glycolate and glyoxylate degradation	33.33	2 of 6
[Ref.: #66794]	4-hydroxymandelate degradation	33.33	3 of 9
[Ref.: #66794]	pantothenate biosynthesis	33.33	2 of 6
[Ref.: #66794]	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	sphingosine metabolism	33.33	2 of 6
[Ref.: #66794]	arachidonic acid metabolism	33.33	6 of 18
[Ref.: #66794]	selenocysteine biosynthesis	33.33	2 of 6
[Ref.: #66794]	degradation of pentoses	32.14	9 of 28
[Ref.: #66794]	urea cycle	30.77	4 of 13
[Ref.: #66794]	polyamine pathway	30.43	7 of 23
[Ref.: #66794]	starch degradation	30	3 of 10
[Ref.: #66794]	coenzyme M biosynthesis	30	3 of 10
[Ref.: #66794]	aclacinomycin biosynthesis	28.57	2 of 7
[Ref.: #66794]	benzoyl-CoA degradation	28.57	2 of 7
[Ref.: #66794]	catecholamine biosynthesis	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	ppGpp biosynthesis	25	1 of 4
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #19194]	Sample type/isolated from	freshwater hot spring
[Ref.: #19194]	Geographic location (country and/or sea, region)	Arkansas, Hot Springs National Park, Hale Bathhouse (W 93° 3' 13'', N 34° 30' 47'')
[Ref.: #19194]	Country	USA
[Ref.: #19194]	Country ISO 3 Code	USA
[Ref.: #19194]	Continent	North America
[Ref.: #19194]	Geographic location	34.5131°/-93.0536°

[Ref.: #62085]	Sample type/isolated from	Hot spring water
[Ref.: #62085]	Sampling date	2010-01-20
[Ref.: #62085]	Geographic location (country and/or sea, region)	Arkansas,Hale Bath House
[Ref.: #62085]	Country	USA
[Ref.: #62085]	Country ISO 3 Code	USA
[Ref.: #62085]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #19194]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #19194]	Fontimonas thermophila strain HA-01 16S ribosomal RNA gene, partial sequence	JN415769	1468	ENA	1076937 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Fontimonas thermophila DSM 23609	GCA_900113085	scaffold	GenBank	1076937 tax ID	*
[Ref.: #66792]	Fontimonas thermophila strain DSM 23609	1076937.3	wgs	PATRIC	1076937 tax ID	*
[Ref.: #66792]	Fontimonas thermophila DSM 23609	2622736503	draft	JGI IMG/M	1076937 tax ID	*

[Ref.: #19194]	GC-content	64.4 mol%	high performance liquid chromatography (HPLC)
[Ref.: #30484]	GC-content	64.4 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Fontimonas thermophila DSM 23609 NCBI: GCA_900113085.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Fontimonas thermophila DSM 23609 NCBI: GCA_900113085.1
[Ref.: #69480]	motile	yes	87.153	yes
[Ref.: #69480]	flagellated	no	64.554	no
[Ref.: #69480]	gram-positive	no	99.116	yes
[Ref.: #69480]	anaerobic	no	97.903	no
[Ref.: #69480]	aerobic	yes	85.253	yes
[Ref.: #69480]	halophile	no	89.97	yes
[Ref.: #69480]	spore-forming	no	94.376	yes
[Ref.: #69480]	glucose-ferment	no	92.108	no
[Ref.: #69480]	thermophile	yes	72.735	yes
[Ref.: #69480]	glucose-util	yes	50.754	no

Availability in culture collections External links

[Ref.: #19194]

Culture collection no.

DSM 23609, CCUG 59713

[Ref.: #87605]

SI-ID 405672

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Flagellatimonas centrodinii gen. nov., sp. nov., a novel member of the family Nevskiaceae isolated from toxin-producing dinoflagellate Centrodinium punctatum.	Jiang Y, Jiang L, Peng Y, Kim KH, Shin HH, Kim YM, Lee J, Li Z	Int J Syst Evol Microbiol	10.1099/ijsem.0.005084	2021	*
Phylogeny	Fontimonas thermophila gen. nov., sp. nov., a moderately thermophilic bacterium isolated from a freshwater hot spring, and proposal of Solimonadaceae fam. nov. to replace Sinobacteraceae Zhou et al. 2008.	Losey NA, Stevenson BS, Verbarg S, Rudd S, Moore ERB, Lawson PA	Int J Syst Evol Microbiol	10.1099/ijs.0.037127-0	2012	*

References

#19194

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23609

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30484

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26818 (see below)

#62085 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 59713

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#87605

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405672.1 )

#26818

IJSEM 254 2013 ( DOI 10.1099/ijs.0.037127-0 , PubMed 22407788 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Fontimonas thermophila in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Fontimonas thermophila DSM 23609

Fontimonas thermophila DSM 23609

Change proposal

Change proposal