Name: Dissulfuribacter thermophilus S69
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 23455

Type strain:

Species: Dissulfuribacter thermophilus

Strain Designation: S69

Culture col. no.: DSM 25762, VKM B-2760

Strain history: <- A. Slobodkin, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; S69 <- A. Slobodkin {2010}

NCBI tax ID(s): 1156395 (species)

SI-ID 398252

DSM 25762

Section

Dissulfuribacter thermophilus S69 is an anaerobe, thermophilic, Gram-negative bacterium that was isolated from deep-sea hydrothermal chimney.

Gram-negative
motile
rod-shaped
anaerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Deltaproteobacteria*
	Order	*Dissulfuribacterales*
	Family	*Dissulfuribacteraceae*
	Genus	*Dissulfuribacter*
	Species	**Dissulfuribacter thermophilus**
	Full Scientific Name (LPSN)	Dissulfuribacter thermophilus Slobodkin et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30893]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.996

[Ref.: #30893]	Cell length	1.75 µm

[Ref.: #30893]	Cell width	0.5 µm

[Ref.: #30893]	Cell shape	rod-shaped

[Ref.: #30893]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %92.821

[Ref.: #30893]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #19341]

Culture medium

THERMOSULFURIMONAS MEDIUM (DSMZ Medium 1210a)

[Ref.: #19341]

Culture medium growth

[Ref.: #19341]

Culture medium link

Medium recipe at MediaDive

[Ref.: #19341]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #19341]	growth	60 °C
[Ref.: #30893]	growth	28-70 °C
[Ref.: #30893]	optimum	61 °C

[Ref.: #19341]	Temperature range	thermophilic
[Ref.: #30893]	Temperature range	thermophilic
[Ref.: #69480]	Temperature range	thermophilic Confidence in %100

	pH	Kind of pH		pH
[Ref.: #30893]			optimum	6.8
[Ref.: #30893]			growth	5.6-7.9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #19341]	Oxygen tolerance	anaerobe
[Ref.: #30893]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.719

[Ref.: #30893]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %99
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.929

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30893]		NaCl		growth	0.9-5.0	%
[Ref.: #30893]		NaCl		optimum	2.25	%

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	glycogen metabolism	100	5 of 5
[Ref.: #66794]	reductive acetyl coenzyme A pathway	100	7 of 7
[Ref.: #66794]	hydrogen production	100	5 of 5
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	1,4-dihydroxy-6-naphthoate biosynthesis	100	6 of 6
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	100	4 of 4
[Ref.: #66794]	biotin biosynthesis	100	4 of 4
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	sulfopterin metabolism	100	4 of 4
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	methylglyoxal degradation	100	5 of 5
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	lipid A biosynthesis	88.89	8 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	palmitate biosynthesis	86.36	19 of 22
[Ref.: #66794]	ubiquinone biosynthesis	85.71	6 of 7
[Ref.: #66794]	photosynthesis	85.71	12 of 14
[Ref.: #66794]	factor 420 biosynthesis	80	4 of 5
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	heme metabolism	78.57	11 of 14
[Ref.: #66794]	tetrahydrofolate metabolism	78.57	11 of 14
[Ref.: #66794]	NAD metabolism	77.78	14 of 18
[Ref.: #66794]	valine metabolism	77.78	7 of 9
[Ref.: #66794]	d-mannose degradation	77.78	7 of 9
[Ref.: #66794]	vitamin B1 metabolism	76.92	10 of 13
[Ref.: #66794]	vitamin B12 metabolism	76.47	26 of 34
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	isoleucine metabolism	75	6 of 8
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	C4 and CAM-carbon fixation	75	6 of 8
[Ref.: #66794]	cardiolipin biosynthesis	71.43	5 of 7
[Ref.: #66794]	purine metabolism	69.15	65 of 94
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	molybdenum cofactor biosynthesis	66.67	6 of 9
[Ref.: #66794]	flavin biosynthesis	66.67	10 of 15
[Ref.: #66794]	serine metabolism	66.67	6 of 9
[Ref.: #66794]	selenocysteine biosynthesis	66.67	4 of 6
[Ref.: #66794]	nitrate assimilation	66.67	6 of 9
[Ref.: #66794]	alanine metabolism	65.52	19 of 29
[Ref.: #66794]	methionine metabolism	65.38	17 of 26
[Ref.: #66794]	pyrimidine metabolism	64.44	29 of 45
[Ref.: #66794]	vitamin B6 metabolism	63.64	7 of 11
[Ref.: #66794]	gluconeogenesis	62.5	5 of 8
[Ref.: #66794]	leucine metabolism	61.54	8 of 13
[Ref.: #66794]	phenylalanine metabolism	61.54	8 of 13
[Ref.: #66794]	threonine metabolism	60	6 of 10
[Ref.: #66794]	creatinine degradation	60	3 of 5
[Ref.: #66794]	glycolysis	58.82	10 of 17
[Ref.: #66794]	isoprenoid biosynthesis	57.69	15 of 26
[Ref.: #66794]	glutamate and glutamine metabolism	57.14	16 of 28
[Ref.: #66794]	citric acid cycle	57.14	8 of 14
[Ref.: #66794]	CO2 fixation in Crenarchaeota	55.56	5 of 9
[Ref.: #66794]	cysteine metabolism	55.56	10 of 18
[Ref.: #66794]	metabolism of disaccharids	54.55	6 of 11
[Ref.: #66794]	sulfate reduction	53.85	7 of 13
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	50	4 of 8
[Ref.: #66794]	denitrification	50	1 of 2
[Ref.: #66794]	myo-inositol biosynthesis	50	5 of 10
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	dTDPLrhamnose biosynthesis	50	4 of 8
[Ref.: #66794]	aminopropanol phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	pantothenate biosynthesis	50	3 of 6
[Ref.: #66794]	adipate degradation	50	1 of 2
[Ref.: #66794]	starch degradation	50	5 of 10
[Ref.: #66794]	phenylmercury acetate degradation	50	1 of 2
[Ref.: #66794]	ketogluconate metabolism	50	4 of 8
[Ref.: #66794]	4-hydroxyphenylacetate degradation	50	5 of 10
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	non-pathway related	47.37	18 of 38
[Ref.: #66794]	degradation of hexoses	44.44	8 of 18
[Ref.: #66794]	propanol degradation	42.86	3 of 7
[Ref.: #66794]	tryptophan metabolism	42.11	16 of 38
[Ref.: #66794]	lipid metabolism	41.94	13 of 31
[Ref.: #66794]	histidine metabolism	41.38	12 of 29
[Ref.: #66794]	ascorbate metabolism	40.91	9 of 22
[Ref.: #66794]	oxidative phosphorylation	40.66	37 of 91
[Ref.: #66794]	lysine metabolism	40.48	17 of 42
[Ref.: #66794]	coenzyme M biosynthesis	40	4 of 10
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	metabolism of amino sugars and derivatives	40	2 of 5
[Ref.: #66794]	arginine metabolism	37.5	9 of 24
[Ref.: #66794]	proline metabolism	36.36	4 of 11
[Ref.: #66794]	pentose phosphate pathway	36.36	4 of 11
[Ref.: #66794]	glutathione metabolism	35.71	5 of 14
[Ref.: #66794]	tyrosine metabolism	35.71	5 of 14
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	33.33	4 of 12
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	glycolate and glyoxylate degradation	33.33	2 of 6
[Ref.: #66794]	L-lactaldehyde degradation	33.33	1 of 3
[Ref.: #66794]	enterobactin biosynthesis	33.33	1 of 3
[Ref.: #66794]	octane oxidation	33.33	1 of 3
[Ref.: #66794]	urea cycle	30.77	4 of 13
[Ref.: #66794]	polyamine pathway	30.43	7 of 23
[Ref.: #66794]	Entner Doudoroff pathway	30	3 of 10
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	butanoate fermentation	25	1 of 4
[Ref.: #66794]	methanogenesis from CO2	25	3 of 12
[Ref.: #66794]	degradation of pentoses	21.43	6 of 28
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #19341]	Sample type/isolated from	deep-sea hydrothermal chimney
[Ref.: #19341]	Geographic location (country and/or sea, region)	Pacific Ocean, Mariner hydrothermal field on the East Lau Spreading Center and Valu Fa Ridge
[Ref.: #19341]	Country	Tonga
[Ref.: #19341]	Country ISO 3 Code	TON
[Ref.: #19341]	Continent	Australia and Oceania
[Ref.: #19341]	Geographic location	-22.1803°/-176.6020°
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Hydrothermal vent
#Environmental	#Aquatic	#Marine
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JQ414031 (>99% sequence identity) for Dissulfuribacter thermophilus subclade from Microbeatlas

Aquatic Samples	272
Soil Samples	27
Animal Samples	11
Plant Samples	4
Total Samples	314

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #19341]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #19341]	Dissulfuribacter thermophilus strain S69 16S ribosomal RNA gene, partial sequence	JQ414031	1502	ENA	1156395 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Dissulfuribacter thermophilus S69	GCA_001687335	contig	GenBank	1156395 tax ID	*
[Ref.: #66792]	Dissulfuribacter thermophilus strain S69	1156395.6	wgs	PATRIC	1156395 tax ID	*
[Ref.: #66792]	Dissulfuribacter thermophilus S69	2724679641	draft	JGI IMG/M	1156395 tax ID	*

[Ref.: #19341]	GC-content	40.5 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #30893]	GC-content	40.5 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Dissulfuribacter thermophilus strain S69 PATRIC: 1156395.6
[Ref.: #69481]	spore-forming	no	99	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Dissulfuribacter thermophilus strain S69 PATRIC: 1156395.6
[Ref.: #69480]	motile	yes	90.105	no
[Ref.: #69480]	gram-positive	no	99.645	no
[Ref.: #69480]	anaerobic	yes	99.999	yes
[Ref.: #69480]	aerobic	no	99.407	yes
[Ref.: #69480]	halophile	no	78.77	no
[Ref.: #69480]	spore-forming	no	95.504	no
[Ref.: #69480]	flagellated	no	78.476	no
[Ref.: #69480]	thermophile	yes	99.188	no
[Ref.: #69480]	glucose-util	no	70.432	no
[Ref.: #69480]	glucose-ferment	no	81.153	no

Availability in culture collections External links

[Ref.: #19341]

Culture collection no.

DSM 25762, VKM B-2760

[Ref.: #87604]

SI-ID 398252

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Dissulfuribacter thermophilus gen. nov., sp. nov., a thermophilic, autotrophic, sulfur-disproportionating, deeply branching deltaproteobacterium from a deep-sea hydrothermal vent.	Slobodkin AI, Reysenbach AL, Slobodkina GB, Kolganova TV, Kostrikina NA, Bonch-Osmolovskaya EA	Int J Syst Evol Microbiol	10.1099/ijs.0.046938-0	2012	*

References

#19341

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25762

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30893

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27223 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#87604

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID398252.1 )

#27223

IJSEM 1967 2013 ( DOI 10.1099/ijs.0.046938-0 , PubMed 23024145 )

* These data were automatically processed and therefore are not curated

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Dissulfuribacter thermophilus strain S69

Dissulfuribacter thermophilus strain S69

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