Name: Brevibacterium pityocampae TP12
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1870

Type strain:

Species: Brevibacterium pityocampae

Strain Designation: TP12

Culture col. no.: DSM 21720, JCM 17808, NCCB 100255

Strain history: DSM 21720 <-- I. A. Ince Tp12.

NCBI tax ID(s): 506594 (species)

SI-ID 401595

DSM 21720

Other strains from Brevibacterium pityocampae

B. pityocampae CCUG 52856 B
B. pityocampae CCUG 52857
B. pityocampae IMI 500470

Section

Brevibacterium pityocampae TP12 is a mesophilic, Gram-positive, rod-shaped bacterium that builds an aerial mycelium and was isolated from healthy larvae of the pine processionary moth Thaumetopoea pityocampa.

Gram-positive
rod-shaped
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Brevibacteriaceae*
	Genus	*Brevibacterium*
	Species	**Brevibacterium pityocampae**
	Full Scientific Name (LPSN)	Brevibacterium pityocampae Kati et al. 2010

Information on morphological and physiological properties Morphology

[Ref.: #29119]	Gram stain	positive

[Ref.: #29119]	Cell length	1 µm

[Ref.: #29119]	Cell width	0.5 µm

[Ref.: #29119]	Cell shape	rod-shaped

[Ref.: #29119]	Motility	no

[Ref.: #21356]	Colony color	Lemon yellow (1012)
[Ref.: #21356]	Incubation period	10-14 days
[Ref.: #21356]	Cultivation medium used	ISP 2

[Ref.: #21356]	Colony color	Sand yellow (1002)
[Ref.: #21356]	Incubation period	10-14 days
[Ref.: #21356]	Cultivation medium used	suter without tyrosine

[Ref.: #21356]	Incubation period	10-14 days
[Ref.: #21356]	Cultivation medium used	ISP 3

[Ref.: #21356]	Incubation period	10-14 days
[Ref.: #21356]	Cultivation medium used	ISP 4

[Ref.: #21356]	Incubation period	10-14 days
[Ref.: #21356]	Cultivation medium used	ISP 5

[Ref.: #21356]	Incubation period	10-14 days
[Ref.: #21356]	Cultivation medium used	ISP 7

[Ref.: #21356]	Incubation period	10-14 days
[Ref.: #21356]	Cultivation medium used	suter with tyrosine

[Ref.: #29119]

Pigment production

yes

[Ref.: #21356]	Multicellular complex forming ability	no
[Ref.: #21356]	Medium Name (multicellularity)	ISP 2

[Ref.: #21356]	Multicellular complex forming ability	no
[Ref.: #21356]	Medium Name (multicellularity)	ISP 3

[Ref.: #21356]	Multicellular complex forming ability	no
[Ref.: #21356]	Medium Name (multicellularity)	ISP 4

[Ref.: #21356]	Multicellular complex forming ability	no
[Ref.: #21356]	Medium Name (multicellularity)	ISP 5

[Ref.: #21356]	Multicellular complex forming ability	no
[Ref.: #21356]	Medium Name (multicellularity)	ISP 7

[Ref.: #21356]	Multicellular complex forming ability	no
[Ref.: #21356]	Medium Name (multicellularity)	Suter with tyrosine

[Ref.: #21356]	Multicellular complex forming ability	yes
[Ref.: #21356]	Multicellular complex name	Aerial Mycelium
[Ref.: #21356]	Multicellular complex color	Light ivory (1015)
[Ref.: #21356]	Medium Name (multicellularity)	Suter without tyrosine

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15892]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #15892]

Culture medium growth

[Ref.: #15892]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15892]

Culture medium composition

expand / minimize

[Ref.: #21356]

Culture medium

ISP 2

[Ref.: #21356]

Culture medium growth

[Ref.: #21356]

Culture medium composition

expand / minimize

[Ref.: #21356]

Culture medium

suter without tyrosine

[Ref.: #21356]

Culture medium growth

[Ref.: #21356]

Culture medium composition

expand / minimize

[Ref.: #21356]

Culture medium

ISP 3

[Ref.: #21356]

Culture medium growth

[Ref.: #21356]

Culture medium composition

expand / minimize

[Ref.: #21356]

Culture medium

ISP 4

[Ref.: #21356]

Culture medium growth

[Ref.: #21356]

Culture medium composition

expand / minimize

[Ref.: #21356]

Culture medium

ISP 5

[Ref.: #21356]

Culture medium growth

[Ref.: #21356]

Culture medium composition

expand / minimize

[Ref.: #21356]

Culture medium

ISP 7

[Ref.: #21356]

Culture medium growth

[Ref.: #21356]

Culture medium composition

expand / minimize

[Ref.: #21356]

Culture medium

suter with tyrosine

[Ref.: #21356]

Culture medium growth

[Ref.: #21356]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #15892]	growth	30 °C
[Ref.: #29119]	growth	25-40 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #15892]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29119]			optimum	8
[Ref.: #29119]			growth	06-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29119]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #21356]		NaCl		maximum	10	%
[Ref.: #29119]		NaCl		growth	0-10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #21356]	arabinose ChEBI	-
[Ref.: #21356]	cellulose ChEBI	-
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #21356]	fructose ChEBI	+
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #21356]	glucose ChEBI	-
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #21356]	mannose ChEBI	+/-
[Ref.: #21356]	myo-inositol ChEBI	-
[Ref.: #29119]	nitrate ChEBI	+	reduction
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #21356]	raffinose ChEBI	-
[Ref.: #21356]	rhamnose ChEBI	+/-
[Ref.: #21356]	sucrose ChEBI	-
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #21356]	xylose ChEBI	+/-
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API Coryne
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API Coryne
[Ref.: #29119]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API Coryne
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #21356]	485	-	-	-	-	-	-	-	-	+	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #21356]	997	not determinedn.d.	-	+	+	-	+	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15892]	Sample type/isolated from	healthy larvae of the pine processionary moth Thaumetopoea pityocampa
[Ref.: #15892]	Host species	Thaumetopoea pityocampa
[Ref.: #15892]	Geographic location (country and/or sea, region)	Middle Black Sea region, Samsun
[Ref.: #15892]	Country	Turkey
[Ref.: #15892]	Country ISO 3 Code	TUR
[Ref.: #15892]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Healthy larvae of Thaumetopoea pityocampa from the Middle Black Sea region of Turkey
[Ref.: #67770]	Host species	Thaumetopoea pityocampa
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host	#Plants	#Tree

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU484189 (>99% sequence identity) for Brevibacterium from Microbeatlas

Aquatic Samples	1936
Soil Samples	1815
Animal Samples	16032
Plant Samples	611
Total Samples	20394

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15892]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15892]	Brevibacterium pityocampae strain Tp12 16S ribosomal RNA gene, partial sequence	EU484189	1402	ENA	506594 tax ID

[Ref.: #15892]	GC-content	69.8 mol%	high performance liquid chromatography (HPLC)
[Ref.: #29119]	GC-content	69.8 mol%

Availability in culture collections External links

[Ref.: #15892]

Culture collection no.

DSM 21720, JCM 17808, NCCB 100255

[Ref.: #71515]

SI-ID 401595

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Brevibacterium daeguense sp. nov., a nitrate-reducing bacterium isolated from a 4-chlorophenol enrichment culture.	Cui Y, Kang MS, Woo SG, Jin L, Kim KK, Park J, Lee M, Lee ST	Int J Syst Evol Microbiol	10.1099/ijs.0.038141-0	2012	*
Phylogeny	Brevibacterium pityocampae sp. nov., isolated from caterpillars of Thaumetopoea pityocampa (Lepidoptera, Thaumetopoeidae).	Kati H, Ince IA, Demir I, Demirbag Z	Int J Syst Evol Microbiol	10.1099/ijs.0.006692-0	2009	*

References

#15892

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21720

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21356

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#29119

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25545 (see below)

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#71515

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401595.1 )

#25545

IJSEM 312 2010 ( DOI 10.1099/ijs.0.006692-0 , PubMed 19651741 )

* These data were automatically processed and therefore are not curated

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