Name: Brevibacterium sandarakinum 01-Je-003
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1871

Type strain:

Species: Brevibacterium sandarakinum

Strain Designation: 01-Je-003

Culture col. no.: DSM 22082, CCM 7649, CCUG 57423, JCM 30489

Strain history: CCM 7649 <-- P. Kämpfer; Justus-Liebig-Univ. Giessen, Germany; 01-Je-003.

NCBI tax ID(s): 629680 (species)

SI-ID 402391

DSM 22082

Section

Brevibacterium sandarakinum 01-Je-003 is a mesophilic, Gram-positive, coccus-shaped bacterium that was isolated from wall of a house colonized with moulds.

coccus-shaped
Gram-positive
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Brevibacteriaceae*
	Genus	*Brevibacterium*
	Species	**Brevibacterium sandarakinum**
	Full Scientific Name (LPSN)	Brevibacterium sandarakinum Kämpfer et al. 2010

Information on morphological and physiological properties Morphology

[Ref.: #29489]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #29489]	Cell width	1 µm

[Ref.: #29489]	Cell shape	coccus-shaped

[Ref.: #29489]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %94.592

[Ref.: #21366]	Colony color	Daffodil yellow (1007)
[Ref.: #21366]	Incubation period	10-14 days
[Ref.: #21366]	Cultivation medium used	ISP 2

[Ref.: #21366]	Colony color	Sun yellow (1037)
[Ref.: #21366]	Incubation period	10-14 days
[Ref.: #21366]	Cultivation medium used	ISP 5

[Ref.: #21366]	Colony color	Sun yellow (1037)
[Ref.: #21366]	Incubation period	10-14 days
[Ref.: #21366]	Cultivation medium used	ISP 7

[Ref.: #21366]	Colony color	Sand yellow (1002)
[Ref.: #21366]	Incubation period	10-14 days
[Ref.: #21366]	Cultivation medium used	suter without tyrosine

[Ref.: #21366]	Incubation period	10-14 days
[Ref.: #21366]	Cultivation medium used	ISP 3

[Ref.: #21366]	Incubation period	10-14 days
[Ref.: #21366]	Cultivation medium used	ISP 4

[Ref.: #21366]	Incubation period	10-14 days
[Ref.: #21366]	Cultivation medium used	suter with tyrosine

[Ref.: #29489]

Pigment production

[Ref.: #21366]	Multicellular complex forming ability	no
[Ref.: #21366]	Medium Name (multicellularity)	ISP 2

[Ref.: #21366]	Multicellular complex forming ability	no
[Ref.: #21366]	Medium Name (multicellularity)	ISP 3

[Ref.: #21366]	Multicellular complex forming ability	no
[Ref.: #21366]	Medium Name (multicellularity)	ISP 4

[Ref.: #21366]	Multicellular complex forming ability	no
[Ref.: #21366]	Medium Name (multicellularity)	ISP 5

[Ref.: #21366]	Multicellular complex forming ability	no
[Ref.: #21366]	Medium Name (multicellularity)	ISP 7

[Ref.: #21366]	Multicellular complex forming ability	no
[Ref.: #21366]	Medium Name (multicellularity)	Suter with tyrosine

[Ref.: #21366]	Multicellular complex forming ability	no
[Ref.: #21366]	Medium Name (multicellularity)	Suter without tyrosine

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16127]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #16127]

Culture medium growth

[Ref.: #16127]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16127]

Culture medium composition

expand / minimize

[Ref.: #21366]

Culture medium

ISP 2

[Ref.: #21366]

Culture medium growth

[Ref.: #21366]

Culture medium composition

expand / minimize

[Ref.: #21366]

Culture medium

ISP 3

[Ref.: #21366]

Culture medium growth

[Ref.: #21366]

Culture medium composition

expand / minimize

[Ref.: #21366]

Culture medium

ISP 4

[Ref.: #21366]

Culture medium growth

[Ref.: #21366]

Culture medium composition

expand / minimize

[Ref.: #21366]

Culture medium

ISP 5

[Ref.: #21366]

Culture medium growth

[Ref.: #21366]

Culture medium composition

expand / minimize

[Ref.: #21366]

Culture medium

ISP 7

[Ref.: #21366]

Culture medium growth

[Ref.: #21366]

Culture medium composition

expand / minimize

[Ref.: #21366]

Culture medium

suter with tyrosine

[Ref.: #21366]

Culture medium growth

[Ref.: #21366]

Culture medium composition

expand / minimize

[Ref.: #21366]

Culture medium

suter without tyrosine

[Ref.: #21366]

Culture medium growth

[Ref.: #21366]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #16127]	growth	28 °C
[Ref.: #29489]	growth	04-36 °C
[Ref.: #29489]	optimum	27.5 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #16127]	Temperature range	mesophilic
[Ref.: #29489]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29489]			optimum	8.5
[Ref.: #29489]			growth	5.5-12.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29489]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.991

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #21366]		NaCl		maximum	10	%
[Ref.: #29489]		NaCl		growth	01-10	%

[Ref.: #67770]

Observation

quinones: MK-8(H₂), MK-7(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29489]	3-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #29489]	acetate ChEBI	+	carbon source
[Ref.: #21366]	arabinose ChEBI	-
[Ref.: #21366]	cellulose ChEBI	+/-
[Ref.: #29489]	citrate ChEBI	+	carbon source
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #21366]	fructose ChEBI	+
[Ref.: #29489]	galactose ChEBI	+	carbon source
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #21366]	glucose ChEBI	+
[Ref.: #29489]	glucose ChEBI	+	carbon source
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #29489]	histidine ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #21366]	mannose ChEBI	+/-
[Ref.: #29489]	mannose ChEBI	+	carbon source
[Ref.: #21366]	myo-inositol ChEBI	-
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #29489]	propionate ChEBI	+	carbon source
[Ref.: #29489]	pyruvate ChEBI	+	carbon source
[Ref.: #21366]	raffinose ChEBI	+/-
[Ref.: #21366]	rhamnose ChEBI	-
[Ref.: #29489]	ribose ChEBI	+	carbon source
[Ref.: #29489]	sorbitol ChEBI	+	carbon source
[Ref.: #21366]	sucrose ChEBI	-
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #21366]	xylose ChEBI	-
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API Coryne
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API Coryne
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #21366]	495	-	+/-	-	-	-	-	-	-	-	-	+	not determinedn.d.	-	-	-	-	-	+/-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #21366]	1007	not determinedn.d.	+/-	+	+	-	+	-	-	+/-	-	-	+/-	-	-	-	-	-	+/-	-	+/-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16127]	Sample type/isolated from	wall of a house colonized with moulds
[Ref.: #16127]	Geographic location (country and/or sea, region)	Jena
[Ref.: #16127]	Country	Germany
[Ref.: #16127]	Country ISO 3 Code	DEU
[Ref.: #16127]	Continent	Europe

[Ref.: #61319]	Sample type/isolated from	Wall of a house colonized with moulds
[Ref.: #61319]	Geographic location (country and/or sea, region)	Jena
[Ref.: #61319]	Country	Germany
[Ref.: #61319]	Country ISO 3 Code	DEU
[Ref.: #61319]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Wall of a house colonized with moulds
[Ref.: #67770]	Geographic location (country and/or sea, region)	Jena
[Ref.: #67770]	Country	Germany
[Ref.: #67770]	Country ISO 3 Code	DEU
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Built environment	#House
#Host	#Fungi	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16127]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16127]	Brevibacterium sandarakinum partial 16S rRNA gene, type strain 01-Je-003T	FN293377	1424	ENA	629680 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Brevibacterium sandarakinum DSM 22082	GCA_900105045	chromosome	GenBank	629680 tax ID	*
[Ref.: #66792]	Brevibacterium sandarakinum strain DSM 22082	629680.3	complete	PATRIC	629680 tax ID	*
[Ref.: #66792]	Brevibacterium sandarakinum DSM 22082	2634166182	draft	JGI IMG/M	629680 tax ID	*

[Ref.: #67770]

GC-content

63.3 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Brevibacterium sandarakinum DSM 22082 NCBI: GCA_900105045.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Brevibacterium sandarakinum strain DSM 22082 PATRIC: 629680.3
[Ref.: #69480]	gram-positive	yes	90.94	yes
[Ref.: #69480]	anaerobic	no	99.466	no
[Ref.: #69480]	halophile	yes	82.067	no
[Ref.: #69480]	spore-forming	no	88.211	no
[Ref.: #69480]	glucose-util	yes	86.511	no
[Ref.: #69480]	thermophile	no	99.241	yes
[Ref.: #69480]	flagellated	no	97.766	yes
[Ref.: #69480]	aerobic	yes	94.945	no
[Ref.: #69480]	motile	no	93.922	yes
[Ref.: #69480]	glucose-ferment	no	92.504	yes

Availability in culture collections External links

[Ref.: #16127]

Culture collection no.

DSM 22082, CCM 7649, CCUG 57423, JCM 30489

[Ref.: #71516]

SI-ID 402391

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Brevibacterium sandarakinum sp. nov., isolated from a wall of an indoor environment.	Kampfer P, Schafer J, Lodders N, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijs.0.014100-0	2009	*

References

#16127

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22082

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21366

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#29489

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25889 (see below)

#61319 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 57423

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71516

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402391.1 )

#25889

IJSEM 909 2010 ( DOI 10.1099/ijs.0.014100-0 , PubMed 19661519 )

* These data were automatically processed and therefore are not curated

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Brevibacterium sandarakinum DSM 22082

Brevibacterium sandarakinum strain DSM 22082

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