Name: Thiomonas intermedia DSM 18155
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17949

Type strain:

Species: Thiomonas intermedia

Culture col. no.: DSM 18155, ATCC 15466, CIP 104401, IAM 12131, IFO 14564, NBRC 14564, NCIMB 9515, JCM 20425

Strain history: CIP <- 1995, IFO <- 1987, IAM <- ATCC <- J. London

NCBI tax ID(s): 926 (species)

SI-ID 10392

LMG 7198, IAM 12131, LMG 8563, ATCC 15466, LMG 8694, CIP 104401, IFO 14564, NCIB 9515, NCIMB 9515, NBRC 14564, DSM 18155, BCRC 17547, CCRC 17547, JCM 20425

Section

Thiomonas intermedia DSM 18155 is a mesophilic, Gram-negative bacterium that was isolated from mud at the bank of a freshwater stream.

Gram-negative
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Comamonadaceae*
	Genus	*Thiomonas*
	Species	**Thiomonas intermedia**
	Full Scientific Name (LPSN)	Thiomonas intermedia (London 1963) Moreira and Amils 1997

Synonym

Thiobacillus intermedius

Information on morphological and physiological properties Morphology

[Ref.: #120147]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %100

[Ref.: #120147]	Cell shape	rod-shaped

[Ref.: #120147]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7339]

Culture medium

THIOMONAS MEDIUM (DSMZ Medium 35a)

[Ref.: #7339]

Culture medium growth

[Ref.: #7339]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7339]

Culture medium composition

expand / minimize

[Ref.: #34702]

Culture medium

MEDIUM 126 - for Thiomonas

[Ref.: #34702]

Culture medium growth

[Ref.: #34702]

Culture medium composition

expand / minimize

[Ref.: #120147]

Culture medium

CIP Medium 126

[Ref.: #120147]

Culture medium growth

[Ref.: #120147]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #7339]		growth	30 °C
[Ref.: #34702]		growth	30 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #120147]		growth	30 °C
[Ref.: #120147]	no	growth	10 °C
[Ref.: #120147]	no	growth	37 °C
[Ref.: #120147]	no	growth	45 °C

[Ref.: #7339]	Temperature range	mesophilic
[Ref.: #34702]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #120147]	Temperature range	mesophilic
[Ref.: #120147]	Temperature range	psychrophilic
[Ref.: #120147]	Temperature range	mesophilic
[Ref.: #120147]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %97
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.999

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #120147]	nitrate ChEBI	-	reduction
[Ref.: #120147]	nitrate ChEBI	-	respiration
[Ref.: #120147]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #120147]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #120147]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #120147]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #120147]	beta-galactosidase	-	3.2.1.23
[Ref.: #120147]	catalase	-	1.11.1.6
[Ref.: #120147]	gelatinase	-
[Ref.: #120147]	lysine decarboxylase	-	4.1.1.18
[Ref.: #120147]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120147]	oxidase	+
[Ref.: #120147]	urease	-	3.5.1.5

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #120147]	9592	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7339]	Sample type/isolated from	mud at the bank of a freshwater stream

[Ref.: #67770]	Sample type/isolated from	Mud

[Ref.: #120147]	Sample type/isolated from	Environment, Soil
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#River (Creek)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY455809 (>99% sequence identity) for Thiomonas from Microbeatlas

Aquatic Samples	2269
Soil Samples	388
Animal Samples	312
Plant Samples	154
Total Samples	3123

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7339]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120147]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7339]	Thiomonas intermedia 16S ribosomal RNA gene, partial sequence	AY455809	1390	ENA	926 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Thiomonas intermedia ATCC 15466	GCA_002028405	complete	GenBank	926 tax ID	*
[Ref.: #66792]	Thiomonas intermedia strain ATCC 15466	926.5	plasmid	PATRIC	926 tax ID	*
[Ref.: #66792]	Thiomonas intermedia strain ATCC 15466	926.3	complete	PATRIC	926 tax ID	*
[Ref.: #66792]	Thiomonas intermedia ATCC 15466	2772190779	complete	JGI IMG/M	926 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Thiomonas intermedia ATCC 15466 NCBI: GCA_002028405.1
[Ref.: #69481]	spore-forming	no	97	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Thiomonas intermedia ATCC 15466 NCBI: GCA_002028405.1
[Ref.: #69480]	flagellated	yes	67.465	no
[Ref.: #69480]	gram-positive	no	97.963	no
[Ref.: #69480]	anaerobic	no	98.544	no
[Ref.: #69480]	aerobic	yes	81.307	no
[Ref.: #69480]	halophile	no	95.928	no
[Ref.: #69480]	spore-forming	no	96.267	no
[Ref.: #69480]	glucose-util	no	59.971	no
[Ref.: #69480]	thermophile	no	96.965	yes
[Ref.: #69480]	motile	yes	91.794	no
[Ref.: #69480]	glucose-ferment	no	88.365	no

Availability in culture collections External links

[Ref.: #7339]

Culture collection no.

DSM 18155, ATCC 15466, CIP 104401, IAM 12131, IFO 14564, NBRC 14564, NCIMB 9515, JCM 20425

[Ref.: #86937]

SI-ID 10392

Literature:

Topic	Title	Authors	Journal	DOI	Year
Cultivation	Key internal factors leading to the variability in CO2 fixation efficiency of different sulfur-oxidizing bacteria during autotrophic cultivation.	Wang YN, Kai Y, Wang L, Tsang YF, Fu X, Hu J, Xie Y	J Environ Manage	10.1016/j.jenvman.2020.110957	2020	*
Metabolism	Response of cbb gene transcription levels of four typical sulfur-oxidizing bacteria to the CO2 concentration and its effect on their carbon fixation efficiency during sulfur oxidation.	Wang YN, Wang L, Tsang YF, Fu X, Hu J, Li H, Le Y	Enzyme Microb Technol	10.1016/j.enzmictec.2016.06.015	2016	*
Phylogeny	Bacteria involved in sulfur amendment oxidation and acidification processes of alkaline 'alperujo' compost.	Garcia-de-la-Fuente R, Cuesta G, Sanchis-Jimenez E, Botella S, Abad M, Fornes F	Bioresour Technol	10.1016/j.biortech.2010.09.103	2010	*
Phylogeny	Thiomonas islandica sp. nov., a moderately thermophilic, hydrogen- and sulfur-oxidizing betaproteobacterium isolated from a hot spring.	Vesteinsdottir H, Reynisdottir DB, Orlygsson J	Int J Syst Evol Microbiol	10.1099/ijs.0.015511-0	2010	*
Phylogeny	Thiomonas bhubaneswarensis sp. nov., an obligately mixotrophic, moderately thermophilic, thiosulfate-oxidizing bacterium.	Panda SK, Jyoti V, Bhadra B, Nayak KC, Shivaji S, Rainey FA, Das SK	Int J Syst Evol Microbiol	10.1099/ijs.0.007120-0	2009	*

References

#7339

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18155

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#34702 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#86937

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID10392.1 )

#120147 Collection of Institut Pasteur ; Curators of the CIP; CIP 104401
* These data were automatically processed and therefore are not curated

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Thiomonas intermedia ATCC 15466

Thiomonas intermedia ATCC 15466

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