Name: Brachyspira murdochii 56-150
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17852

Type strain:

Species: Brachyspira murdochii

Strain Designation: 56-150

Culture col. no.: DSM 12563, ATCC 51284, CIP 105832

Strain history: CIP <- 1999, T. Stanton, NADC, Iowa, USA: strain 56-150 <- D. Hampson

NCBI tax ID(s): 526224 (strain), 84378 (species)

SI-ID 42880

ATCC 51284, DSM 12563, CIP 105832

Section

Brachyspira murdochii 56-150 is an anaerobe, mesophilic, Gram-negative animal pathogen that was isolated from pig.

Gram-negative
anaerobe
animal pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Spirochaetota*
	Class	*Spirochaetia*
	Order	*Brachyspirales*
	Family	*Brachyspiraceae*
	Genus	*Brachyspira*
	Species	**Brachyspira murdochii**
	Full Scientific Name (LPSN)	Brachyspira murdochii (Stanton et al. 1997) Hampson and La 2006

Synonym

Serpulina murdochii

Information on morphological and physiological properties Morphology

[Ref.: #121192]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.877

[Ref.: #121192]	Cell shape	rod-shaped

[Ref.: #121192]	Motility	yes

[Ref.: #121192]	Hemolysis ability	1

[Ref.: #4674]	Incubation period	1-2 days

Multimedia content

[Ref.: #66793]

Caption:

electron microscopic image

[Ref.: #66793]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4674]

Culture medium

SERPULINA MURDOCHII MEDIUM (DSMZ Medium 840)

[Ref.: #4674]

Culture medium growth

[Ref.: #4674]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4674]

Culture medium composition

expand / minimize

[Ref.: #41740]

Culture medium

MEDIUM 45 - for Columbia agar with sheep blood

[Ref.: #41740]

Culture medium growth

[Ref.: #41740]

Culture medium composition

expand / minimize

[Ref.: #4674]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #4674]

Culture medium growth

[Ref.: #4674]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4674]

Culture medium composition

expand / minimize

[Ref.: #121192]

Culture medium

CIP Medium 45

[Ref.: #121192]

Culture medium growth

[Ref.: #121192]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #4674]			growth	37 °C
[Ref.: #41740]			growth	37 °C

[Ref.: #4674]	Temperature range	mesophilic
[Ref.: #41740]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #4674]	Oxygen tolerance	anaerobe
[Ref.: #121192]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.588

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.931

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68373]	acetate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	citrate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	D-glucose ChEBI	-	assimilation	* from API CAM
[Ref.: #121192]	esculin ChEBI	-	hydrolysis
[Ref.: #121192]	hippurate ChEBI	-	hydrolysis
[Ref.: #68373]	hippurate ChEBI	-	hydrolysis	* from API CAM
[Ref.: #68373]	malate ChEBI	-	assimilation	* from API CAM
[Ref.: #121192]	nitrate ChEBI	-	reduction
[Ref.: #68373]	nitrate ChEBI	-	reduction	* from API CAM
[Ref.: #121192]	nitrite ChEBI	-	reduction
[Ref.: #68373]	propionate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	succinate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	triphenyltetrazolium chloride ChEBI	-	reduction	* from API CAM
[Ref.: #68373]	urea ChEBI	-	hydrolysis	* from API CAM
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #68373]	cefazolin ChEBI	yes	224	µg	* from API CAM
[Ref.: #68373]	erythromycin ChEBI	yes	14	µg	* from API CAM
[Ref.: #68373]	nalidixic acid ChEBI	yes	84	µg	* from API CAM

	Metabolite production	Metabolite	Production
[Ref.: #121192]		indole ChEBI	no
[Ref.: #68373]		hydrogen sulfide ChEBI	no	* from API CAM

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68373]	alkaline phosphatase	-	3.1.3.1	* from API CAM
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121192]	amylase	-
[Ref.: #121192]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121192]	caseinase	-	3.4.21.50
[Ref.: #121192]	catalase	-	1.11.1.6
[Ref.: #68373]	catalase	-	1.11.1.6	* from API CAM
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #121192]	DNase	-
[Ref.: #68373]	esterase	+		* from API CAM
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68373]	gamma-glutamyltransferase	-	2.3.2.2	* from API CAM
[Ref.: #121192]	gelatinase	-
[Ref.: #68373]	L-arginine arylamidase	-		* from API CAM
[Ref.: #68373]	L-aspartate arylamidase	-	3.4.11.21	* from API CAM
[Ref.: #121192]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #121192]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #121192]	oxidase	-
[Ref.: #121192]	protease	-
[Ref.: #68373]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API CAM
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121192]	tryptophan deaminase	-
[Ref.: #121192]	urease	-	3.5.1.5
[Ref.: #68373]	urease	-	3.5.1.5	* from API CAM
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® CAM:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Reduction of nitrateNIT	EsteraseEST	Hippuric acidHIP	gamma-Glutamyl transferaseGGT	Reduction of Triphenyl tetrazolium chlorideTTC	Pyrrolidonyl arylamidasePYRA	L-arginine arylamidaseArgA	L-aspartic acid arylamidaseAspA	Alkaline phosphatasePAL	H2S productionH2S	Assimilation of D-glucoseGLU	Assimilation of succinateSUT	Growth inhibitionNAL	Growth inhibitionCFZ	Assimilation of acetateACE	Assimilation of propionic acidPROP	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Erythromycin resistance (+) sensitivity (-)ERO	CatalaseCAT
[Ref.: #4674]	3909	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121192]	9031	-	+	+	+	-	-	-	-	-	-	+	-	-	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4674]	Sample type/isolated from	pig
[Ref.: #4674]	Geographic location (country and/or sea, region)	Quebec
[Ref.: #4674]	Country	Canada
[Ref.: #4674]	Country ISO 3 Code	CAN
[Ref.: #4674]	Continent	North America

[Ref.: #121192]	Sample type/isolated from	Animal, Swine, intestinal contents
[Ref.: #121192]	Country	Australia
[Ref.: #121192]	Country ISO 3 Code	AUS
[Ref.: #121192]	Continent	Australia and Oceania
[Ref.: #121192]	Isolation date	1992
* marker position based on {}

Isolation sources categories

#Host

#Mammals

#Suidae (Pig,Swine)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY312492 (>99% sequence identity) for Brachyspira from Microbeatlas

Aquatic Samples	339
Soil Samples	105
Animal Samples	11362
Plant Samples	43
Total Samples	11849

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4674]	Pathogenicity (animal)	yes
[Ref.: #4674]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121192]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #4674]	Brachyspira murdochii strain 56-150 16S ribosomal RNA gene, partial sequence	AY312492	1441	ENA	526224 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Brachyspira murdochii DSM 12563	GCA_000092845	complete	GenBank	526224 tax ID	*
[Ref.: #66792]	Brachyspira murdochii DSM 12563	526224.6	complete	PATRIC	526224 tax ID	*
[Ref.: #66792]	Brachyspira murdochii 56-150, DSM 12563	646564514	complete	JGI IMG/M	526224 tax ID	*

[Ref.: #4674]

GC-content

27 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Brachyspira murdochii DSM 12563 DSM 12563 NCBI: GCA_000092845.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Brachyspira murdochii DSM 12563 DSM 12563 NCBI: GCA_000092845.1
[Ref.: #69480]	motile	yes	70.582	no
[Ref.: #69480]	flagellated	no	82.702	no
[Ref.: #69480]	gram-positive	no	96.319	no
[Ref.: #69480]	anaerobic	yes	98.404	no
[Ref.: #69480]	aerobic	no	98.909	no
[Ref.: #69480]	halophile	no	84.222	no
[Ref.: #69480]	spore-forming	no	96.07	no
[Ref.: #69480]	glucose-util	yes	62.234	yes
[Ref.: #69480]	thermophile	no	69.913	yes
[Ref.: #69480]	glucose-ferment	yes	54.51	no

Availability in culture collections External links

[Ref.: #4674]

Culture collection no.

DSM 12563, ATCC 51284, CIP 105832

[Ref.: #86843]

SI-ID 42880

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete genome sequence of Brachyspira murdochii type strain (56-150).	Pati A, Sikorski J, Gronow S, Munk C, Lapidus A, Copeland A, Glavina Del Tio T, Nolan M, Lucas S, Chen F, Tice H, Cheng JF, Han C, Detter JC, Bruce D, Tapia R, Goodwin L, Pitluck S, Liolios K, Ivanova N, Mavromatis K, Mikhailova N, Chen A, Palaniappan K, Land M, Hauser L, Chang YJ, Jeffries CD, Spring S, Rohde M, Goker M, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP	Stand Genomic Sci	10.4056/sigs.831993	2010	*
Phylogeny	Reclassification of Serpulina intermedia and Serpulina murdochii in the genus Brachyspira as Brachyspira intermedia comb. nov. and Brachyspira murdochii comb. nov.	Hampson DJ, La T	Int J Syst Evol Microbiol	10.1099/ijs.0.64004-0	2006	*
Phylogeny	Recognition of two new species of intestinal spirochetes: Serpulina intermedia sp. nov. and Serpulina murdochii sp. nov.	Stanton TB, Fournie-Amazouz E, Postic D, Trott DJ, Grimont PA, Baranton G, Hampson DJ, Saint Girons I	Int J Syst Bacteriol	10.1099/00207713-47-4-1007	1997	*

References

#4674

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12563

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#41740 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66793

Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )

#68373

Automatically annotated from API CAM .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#86843

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID42880.1 )

#121192 Collection of Institut Pasteur ; Curators of the CIP; CIP 105832
* These data were automatically processed and therefore are not curated

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Brachyspira murdochii DSM 12563 DSM 12563

Brachyspira murdochii DSM 12563 DSM 12563

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