Brachyspira murdochii (DSM 12563, ATCC 51284, CIP 105832)

Name: Brachyspira murdochii (DSM 12563, ATCC 51284, CIP 105832)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 17852

Type strain

Strain Designation 56-150

Culture col. no. DSM 12563 ATCC 51284 CIP 105832 56-150

NCBI tax ID(s) 526224 84378

Special Collections DSMZ CIP

History <- ATCC <- D.J. Hampson <- R. Higgins; 56-150 CIP <- 1999, T. Stanton, NADC, Iowa, USA: strain 56-150 <- D. Hampson

Links

Brachyspira murdochii 56-150 is an anaerobe, mesophilic, Gram-negative animal pathogen that was isolated from pig.

Gram-negative motile rod-shaped anaerobe mesophilic animal pathogen genome sequence 16S sequence

Name and taxonomic classification

SI-ID 42880

ATCC 51284,DSM 12563,CIP 105832

@ref 20215

Last LPSN update 2026-02-09 11:22:19

Domain Bacteria

Phylum Spirochaetota

Class Spirochaetia

Order Brachyspirales

Family Brachyspiraceae

Genus Brachyspira

Species Brachyspira murdochii

Full scientific name Brachyspira murdochii (Stanton et al. 1997) Hampson and La 2006

Synonyms (1)

Serpulina murdochii

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
125439	negative			98.6
121192	negative	rod-shaped

Colony morphology

@ref	Incubation period	Hemolysis ability
4674	1-2 days
121192		1

Multimedia

@ref:	66793
multimedia content:	EM_DSM_12563_1.jpg
multimedia.multimedia content:	EM_DSM_12563_1.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
4674	SERPULINA MURDOCHII MEDIUM (DSMZ Medium 840)	Medium recipe at MediaDive	Name: SERPULINA MURDOCHII MEDIUM (DSMZ Medium 840) Composition: NaHCO3 0.03 g/l Glucose 0.015 g/l Bovine serum Air Trypticase soy broth
41740	MEDIUM 45 - for Columbia agar with sheep blood		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Sheepblood (100.000 ml)
4674	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
121192	CIP Medium 45	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
4674	positive	growth	37	mesophilic
41740	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
4674	anaerobe
121192	anaerobe

Spore formation

@ref	Spore formation	Confidence
125439		94.1

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68373	30089 ChEBI	acetate	-	assimilation	from API CAM
68373	16947 ChEBI	citrate	-	assimilation	from API CAM
68373	17634 ChEBI	D-glucose	-	assimilation	from API CAM
121192	4853 ChEBI	esculin	-	hydrolysis
68373	606565 ChEBI	hippurate	-	hydrolysis	from API CAM
121192	606565 ChEBI	hippurate	-	hydrolysis
68373	25115 ChEBI	malate	-	assimilation	from API CAM
68373	17632 ChEBI	nitrate	-	reduction	from API CAM
121192	17632 ChEBI	nitrate	-	reduction
121192	16301 ChEBI	nitrite	-	reduction
68373	17272 ChEBI	propionate	-	assimilation	from API CAM
68373	30031 ChEBI	succinate	-	assimilation	from API CAM
68373	78019 ChEBI	triphenyltetrazolium chloride	-	reduction	from API CAM
68373	16199 ChEBI	urea	-	hydrolysis	from API CAM

Antibiotic resistance

@ref	ChEBI	Metabolite	Sensitivity conc.
68373	474053	cefazolin	224 µg	from API CAM
68373	48923	erythromycin	14 µg	from API CAM
68373	100147	nalidixic acid	84 µg	from API CAM

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68373	16136 ChEBI	hydrogen sulfide		from API CAM
121192	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68373	alkaline phosphatase	-	3.1.3.1	from API CAM
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
121192	amylase	-
68382	beta-galactosidase	+	3.2.1.23	from API zym
121192	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
121192	caseinase	-	3.4.21.50
121192	catalase	-	1.11.1.6
68373	catalase	-	1.11.1.6	from API CAM
68382	cystine arylamidase	-	3.4.11.3	from API zym
121192	DNase	-
68373	esterase	+		from API CAM
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68373	gamma-glutamyltransferase	-	2.3.2.2	from API CAM
121192	gelatinase	-
68373	L-arginine arylamidase	-		from API CAM
68373	L-aspartate arylamidase	-	3.4.11.21	from API CAM
121192	lecithinase	-
68382	leucine arylamidase	-	3.4.11.1	from API zym
121192	lipase	-
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
121192	oxidase	-
121192	protease	-
68373	pyrrolidonyl arylamidase	-	3.4.19.3	from API CAM
68382	trypsin	-	3.4.21.4	from API zym
121192	tryptophan deaminase	-
121192	urease	-	3.5.1.5
68373	urease	-	3.5.1.5	from API CAM
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121192	-	+	+	+	-	-	-	-	-	-	+	-	-	+	-	+	+	-	-	-

API CAM

@ref	URE	Reduction of nitrateNIT	EsteraseEST	HIP	GGT	TTC	PYRA	L-arginine arylamidaseArgA	L-aspartic acid arylamidaseAspA	PAL	H2S productionH2S	GLU	SUT	NAL	CFZ	ACE	PROP	MLT	CIT	Erythromycin resistance (+) sensitivity (-)ERO	CAT
4674	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Suidae (Pig,Swine)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
4674	pig	Quebec	Canada	CAN	North America
121192	Animal, Swine, intestinal contents		Australia	AUS	Australia and Oceania	1992

Taxonmaps

69479

Global distribution of 16S sequence AY312492 (>99% sequence identity) for Brachyspira from Microbeatlas

Aquatic Samples	339
Soil Samples	105
Animal Samples	11362
Plant Samples	43
Total Samples	11849

Interaction and safety

Risk assessment

@ref	Pathogenicity animal	Biosafety level	Biosafety level comment
4674		2	Risk group (German classification) According to TRBA 466
121192		2	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM9284v1 assembly for Brachyspira murdochii DSM 12563	complete	GCA_000092845	526224.6	646564514	526224	99.36

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
4674	Brachyspira murdochii strain 56-150 16S ribosomal RNA gene, partial sequence	AY312492	1441		526224

GC content

@ref	GC-content (mol%)	Method
4674	27	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Brachyspira murdochii DSM 12563
NCBI: GCA_000092845

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	94.10	no
125439	motility	BacteriaNetⓘ	no	54.20	no
125439	gram_stain	BacteriaNetⓘ	negative	98.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	76.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Brachyspira murdochii DSM 12563 DSM 12563
NCBI: GCA_000092845.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	89.39	no
125438	anaerobic	anaerobicⓘ	yes	83.98	yes
125438	aerobic	aerobicⓘ	no	91.72	yes
125438	spore-forming	spore-formingⓘ	no	83.85	no
125438	thermophilic	thermophileⓘ	no	75.72	no
125438	flagellated	motile2+ⓘ	yes	52.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Arabinoxylan and Pectin Metabolism in Crohn's Disease Microbiota: An In Silico Study.	Sabater C, Calvete-Torre I, Ruiz L, Margolles A.	Int J Mol Sci	10.3390/ijms23137093	2022
Phylogeny	Evidence of homologous recombination as a driver of diversity in Brachyspira pilosicoli.	Pandey A, Humbert MV, Jackson A, Passey JL, Hampson DJ, Cleary DW, La Ragione RM, Christodoulides M.	Microb Genom	10.1099/mgen.0.000470	2020
	Veillonella, Firmicutes: Microbes disguised as Gram negatives.	Vesth T, Ozen A, Andersen SC, Kaas RS, Lukjancenko O, Bohlin J, Nookaew I, Wassenaar TM, Ussery DW.	Stand Genomic Sci	10.4056/sigs.2981345	2013
	Structural properties of 2/2 hemoglobins: the group III protein from Helicobacter hepaticus.	Nothnagel HJ, Winer BY, Vuletich DA, Pond MP, Lecomte JT.	IUBMB Life	10.1002/iub.430	2011
	The complete genome sequence of the pathogenic intestinal spirochete Brachyspira pilosicoli and comparison with other Brachyspira genomes.	Wanchanthuek P, Bellgard MI, La T, Ryan K, Moolhuijzen P, Chapman B, Black M, Schibeci D, Hunter A, Barrero R, Phillips ND, Hampson DJ.	PLoS One	10.1371/journal.pone.0011455	2010
Metabolism	Genome mining for ribosomally synthesized and post-translationally modified peptides (RiPPs) in anaerobic bacteria.	Letzel AC, Pidot SJ, Hertweck C.	BMC Genomics	10.1186/1471-2164-15-983	2014
Metabolism	Molecular Evolution of the Oxygen-Binding Hemerythrin Domain.	Alvarez-Carreno C, Becerra A, Lazcano A.	PLoS One	10.1371/journal.pone.0157904	2016
	Genomic Sequencing of High-Efficiency Transducing Streptococcal Bacteriophage A25: Consequences of Escape from Lysogeny.	McCullor K, Postoak B, Rahman M, King C, McShan WM.	J Bacteriol	10.1128/jb.00358-18	2018
	Ankyrin domains across the Tree of Life.	Jernigan KK, Bordenstein SR.	PeerJ	10.7717/peerj.264	2014
Genetics	Direct comparisons of Illumina vs. Roche 454 sequencing technologies on the same microbial community DNA sample.	Luo C, Tsementzi D, Kyrpides N, Read T, Konstantinidis KT.	PLoS One	10.1371/journal.pone.0030087	2012
Metabolism	Identification of surprisingly diverse type IV pili, across a broad range of gram-positive bacteria.	Imam S, Chen Z, Roos DS, Pohlschroder M.	PLoS One	10.1371/journal.pone.0028919	2011
Phylogeny	Bioinformatic characterization of the 4-Toluene Sulfonate Uptake Permease (TSUP) family of transmembrane proteins.	Shlykov MA, Zheng WH, Chen JS, Saier MH.	Biochim Biophys Acta	10.1016/j.bbamem.2011.12.005	2012
Genetics	Complete genome sequence of Brachyspira murdochii type strain (56-150).	Pati A, Sikorski J, Gronow S, Munk C, Lapidus A, Copeland A, Glavina Del Tio T, Nolan M, Lucas S, Chen F, Tice H, Cheng JF, Han C, Detter JC, Bruce D, Tapia R, Goodwin L, Pitluck S, Liolios K, Ivanova N, Mavromatis K, Mikhailova N, Chen A, Palaniappan K, Land M, Hauser L, Chang YJ, Jeffries CD, Spring S, Rohde M, Goker M, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP	Stand Genomic Sci	10.4056/sigs.831993	2010
Phylogeny	Reclassification of Serpulina intermedia and Serpulina murdochii in the genus Brachyspira as Brachyspira intermedia comb. nov. and Brachyspira murdochii comb. nov.	Hampson DJ, La T	Int J Syst Evol Microbiol	10.1099/ijs.0.64004-0	2006
Phylogeny	Recognition of two new species of intestinal spirochetes: Serpulina intermedia sp. nov. and Serpulina murdochii sp. nov.	Stanton TB, Fournie-Amazouz E, Postic D, Trott DJ, Grimont PA, Baranton G, Hampson DJ, Saint Girons I	Int J Syst Bacteriol	10.1099/00207713-47-4-1007	1997

References

#4674	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12563
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#41740	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66793	Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )
#68373	Automatically annotated from API CAM .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121192	Collection of Institut Pasteur ; Curators of the CIP; CIP 105832
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Brachyspira murdochii (DSM 12563, ATCC 51284, CIP 105832)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Antibiotic resistance

Metabolite production

Enzymes

API zym

API CAM

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000092845

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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