Methanolinea tarda (DSM 16494, JCM 12467, NBRC 102358.)

Name: Methanolinea tarda (DSM 16494, JCM 12467, NBRC 102358.)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 17853

Type strain

Strain Designation NOBi-1

Culture col. no. DSM 16494 JCM 12467 NBRC 102358. NOBi-1 NBRC 102358

NCBI tax ID(s) 882090 263906

Special Collections DSMZ JCM Literature strains

History <- H. Imachi, Nagaoka University of Technology, Japan; NOBI-1 <- H. Imachi; {2003} H. Imachi NOBI-1.

Links

Methanolinea tarda NOBi-1 is an anaerobe, Gram-negative, rod-shaped archaeon that was isolated from anaerobic digested sludge.

Gram-negative rod-shaped anaerobe genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 323978

DSM 16494,JCM 12467,NBRC 102358

@ref 20215

Last LPSN update 2026-02-09 11:22:19

Domain Archaea

Phylum Methanobacteriota

Class "Methanomicrobia"

Order Methanomicrobiales

Family Methanoregulaceae

Genus Methanolinea

Species Methanolinea tarda

Full scientific name Methanolinea tarda Imachi et al. 2008

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Confidence
32372	negative	2 µm	0.85 µm	rod-shaped
125438						91.275

Colony morphology

6376

Incubation period>14 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
6376	PELOTOMACULUM MEDIUM (DSMZ Medium 960)		Medium recipe at MediaDive	Name: PELOTOMACULUM MEDIUM (DSMZ Medium 960; with strain-specific modifications) Composition: Na-formate 2.71457 g/l Na2CO3 1.49701 g/l NH4Cl 0.538922 g/l Na2S x 9 H2O 0.299401 g/l L-Cysteine HCl x H2O 0.299401 g/l MgCl2 x 6 H2O 0.199601 g/l KH2PO4 0.139721 g/l Yeast extract 0.0998004 g/l Na acetate 0.0998004 g/l 2-Mercaptoethanesulfonic acid 0.0798403 g/l Nitrilotriacetic acid 0.0127745 g/l FeCl2 x 4 H2O 0.000998004 g/l NaCl 0.000998004 g/l Sodium resazurin 0.000499002 g/l ZnCl2 9.98004e-05 g/l Pyridoxine hydrochloride 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l CaCl2 x 2 H2O 9.98004e-05 g/l NiCl2 x 6 H2O 9.98004e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l Na2WO4 x 2 H2O 3.99202e-05 g/l CoCl2 x 6 H2O 2.99401e-05 g/l Na2MoO4 x 2 H2O 2.99401e-05 g/l Na2SeO3 x 5 H2O 2.99401e-05 g/l CuCl2 1.99601e-05 g/l Folic acid 1.99601e-05 g/l Biotin 1.99601e-05 g/l H3BO3 9.98004e-06 g/l Vitamin B12 9.98004e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
6376	positive	growth	50
32372	positive	growth	35-55
32372	positive	optimum	50
67770	positive	growth	50

Culture pH

@ref	Ability	Type	PH
32372	positive	optimum	7
32372	positive	growth	6.7-8

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
6376	anaerobe
32372	anaerobe

Spore formation

@ref	Spore formation	Confidence
125438		92.161

Halophily

@ref	Salt	Growth	Tested relation	Concentration
32372	NaCl	positive	growth	0-1.5 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
32372	30089 ChEBI	acetate	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
32372	16183 ChEBI	methane

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Mud (Sludge)
#Condition	#Anoxic (anaerobic)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6376	anaerobic digested sludge	Niigata, Nagaoka	Japan	JPN	Asia
67770	Mesophilic methanogenic sludge digesting municipal sewage sludge

Taxonmaps

69479

Global distribution of 16S sequence AB162774 (>99% sequence identity) for Methanolinea tarda subclade from Microbeatlas

Aquatic Samples	308
Soil Samples	50
Animal Samples	13
Plant Samples	32
Total Samples	403

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6376	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID
66792	ASM23568v1 assembly for Methanolinea tarda NOBI-1	scaffold	GCA_000235685	882090.7	882090

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6376	Methanolinea tarda gene for 16S ribosomal RNA, partial sequence	AB162774	1433		882090

GC content

@ref	GC-content (mol%)	Method
32372	56.3
67770	56.3	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methanolinea tarda NOBI-1
PATRIC: 882090.7

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	71.21	no
125438	anaerobic	anaerobicⓘ	yes	89.89	yes
125438	aerobic	aerobicⓘ	no	92.76	yes
125438	spore-forming	spore-formingⓘ	no	92.16	no
125438	thermophilic	thermophileⓘ	yes	51.69	yes
125438	flagellated	motile2+ⓘ	no	91.28	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanolinea tarda gen. nov., sp. nov., a methane-producing archaeon isolated from a methanogenic digester sludge.	Imachi H, Sakai S, Sekiguchi Y, Hanada S, Kamagata Y, Ohashi A, Harada H	Int J Syst Evol Microbiol	10.1099/ijs.0.65394-0	2008

References

#6376	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16494
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#28600	IJSEM 294 2008 ( DOI 10.1099/ijs.0.65394-0 , PubMed 18175725 )
#32372	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28600
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanolinea tarda (DSM 16494, JCM 12467, NBRC 102358.)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000235685

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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