Strain identifier

BacDive ID: 17852

Type strain: Yes

Species: Brachyspira murdochii

Strain Designation: 56-150

Strain history: CIP <- 1999, T. Stanton, NADC, Iowa, USA: strain 56-150 <- D. Hampson

NCBI tax ID(s): 526224 (strain), 84378 (species)

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General

@ref: 4674

BacDive-ID: 17852

DSM-Number: 12563

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, animal pathogen

description: Brachyspira murdochii 56-150 is an anaerobe, mesophilic, Gram-negative animal pathogen that was isolated from pig.

NCBI tax id

NCBI tax idMatching level
526224strain
84378species

strain history

@refhistory
4674<- ATCC <- D.J. Hampson <- R. Higgins; 56-150
121192CIP <- 1999, T. Stanton, NADC, Iowa, USA: strain 56-150 <- D. Hampson

doi: 10.13145/bacdive17852.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/spirochaetota
  • domain: Bacteria
  • phylum: Spirochaetota
  • class: Spirochaetia
  • order: Brachyspirales
  • family: Brachyspiraceae
  • genus: Brachyspira
  • species: Brachyspira murdochii
  • full scientific name: Brachyspira murdochii (Stanton et al. 1997) Hampson and La 2006

  • synonyms

    • @ref: 20215
    • synonym: Serpulina murdochii

@ref: 4674

domain: Bacteria

phylum: Spirochaetes

class: Spirochaetia

order: Spirochaetales

family: Brachyspiraceae

genus: Brachyspira

species: Brachyspira murdochii

full scientific name: Brachyspira murdochii (Stanton et al. 1997) Hampson and La 2006

strain designation: 56-150

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.877
121192negativerod-shapedyes

colony morphology

@refincubation periodhemolysis ability
46741-2 days
1211921

multimedia

  • @ref: 66793
  • multimedia content: EM_DSM_12563_1.jpg
  • caption: electron microscopic image
  • intellectual property rights: © HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4674SERPULINA MURDOCHII MEDIUM (DSMZ Medium 840)yeshttps://mediadive.dsmz.de/medium/840Name: SERPULINA MURDOCHII MEDIUM (DSMZ Medium 840) Composition: NaHCO3 0.03 g/l Glucose 0.015 g/l Bovine serum Air Trypticase soy broth
41740MEDIUM 45 - for Columbia agar with sheep bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Sheepblood (100.000 ml)
4674COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
121192CIP Medium 45yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=45

culture temp

@refgrowthtypetemperaturerange
4674positivegrowth37mesophilic
41740positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
4674anaerobe
69480anaerobe99.588
121192anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.931

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1211924853esculin-hydrolysis
121192606565hippurate-hydrolysis
12119217632nitrate-reduction
12119216301nitrite-reduction
6837316199urea-hydrolysis
6837317632nitrate-reduction
68373606565hippurate-hydrolysis
6837378019triphenyltetrazolium chloride-reduction
6837317634D-glucose-assimilation
6837330031succinate-assimilation
6837330089acetate-assimilation
6837317272propionate-assimilation
6837325115malate-assimilation
6837316947citrate-assimilation

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.
6837348923erythromycinyesyes14 µg
68373474053cefazolinyesyes224 µg
68373100147nalidixic acidyesyes84 µg

metabolite production

@refChebi-IDmetaboliteproduction
6837316136hydrogen sulfideno
12119235581indoleno

enzymes

@refvalueactivityec
68373catalase-1.11.1.6
68373alkaline phosphatase-3.1.3.1
68373L-aspartate arylamidase-3.4.11.21
68373L-arginine arylamidase-
68373pyrrolidonyl arylamidase-3.4.19.3
68373gamma-glutamyltransferase-2.3.2.2
68373esterase+
68373urease-3.5.1.5
121192oxidase-
121192beta-galactosidase+3.2.1.23
121192gelatinase-
121192amylase-
121192DNase-
121192caseinase-3.4.21.50
121192catalase-1.11.1.6
121192lecithinase-
121192lipase-
121192protease-
121192tryptophan deaminase-
121192urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121192-+++------+--+-++---

API CAM

@refURENITESTHIPGGTTTCPYRAArgAAspAPALH2SGLUSUTNALCFZACEPROPMLTCITEROCAT
4674--+------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
4674pigQuebecCanadaCANNorth America
121192Animal, Swine, intestinal contentsAustraliaAUSAustralia and Oceania1992

isolation source categories

  • Cat1: #Host
  • Cat2: #Mammals
  • Cat3: #Suidae (Pig,Swine)

taxonmaps

  • @ref: 69479
  • File name: preview.99_235.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_69;96_166;97_179;98_200;99_235&stattab=map
  • Last taxonomy: Brachyspira
  • 16S sequence: AY312492
  • Sequence Identity:
  • Total samples: 11849
  • soil counts: 105
  • aquatic counts: 339
  • animal counts: 11362
  • plant counts: 43

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
4674yes2Risk group (German classification)
1211922Risk group (French classification)

Sequence information

16S sequences

  • @ref: 4674
  • description: Brachyspira murdochii strain 56-150 16S ribosomal RNA gene, partial sequence
  • accession: AY312492
  • length: 1441
  • database: ena
  • NCBI tax ID: 526224

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brachyspira murdochii DSM 12563GCA_000092845completencbi526224
66792Brachyspira murdochii DSM 12563526224.6completepatric526224
66792Brachyspira murdochii 56-150, DSM 12563646564514completeimg526224

GC content

  • @ref: 4674
  • GC-content: 27
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes70.582no
flagellatedno82.702no
gram-positiveno96.319no
anaerobicyes98.404no
aerobicno98.909no
halophileno84.222no
spore-formingno96.07no
glucose-utilyes62.234yes
thermophileno69.913yes
glucose-fermentyes54.51no

External links

@ref: 4674

culture collection no.: DSM 12563, ATCC 51284, CIP 105832

straininfo link

  • @ref: 86843
  • straininfo: 42880

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny9336899Recognition of two new species of intestinal spirochetes: Serpulina intermedia sp. nov. and Serpulina murdochii sp. nov.Stanton TB, Fournie-Amazouz E, Postic D, Trott DJ, Grimont PA, Baranton G, Hampson DJ, Saint Girons IInt J Syst Bacteriol10.1099/00207713-47-4-10071997Bacterial Proteins/metabolism, Base Composition, Brachyspira/*classification/genetics/growth & development/*metabolism, Culture Media, Conditioned/metabolism, DNA, Bacterial/analysis, Humans, Phenotype, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Spirochaetales/classificationMetabolism
Phylogeny16627646Reclassification of Serpulina intermedia and Serpulina murdochii in the genus Brachyspira as Brachyspira intermedia comb. nov. and Brachyspira murdochii comb. nov.Hampson DJ, La TInt J Syst Evol Microbiol10.1099/ijs.0.64004-02006Base Composition, Brachyspira/*classification/enzymology/genetics/physiology, DNA, Bacterial/chemistry/genetics, Genes, rRNA, Phenotype, Phylogeny, Spirochaetales/*classification/enzymology/genetics/physiologyEnzymology
Genetics21304710Complete genome sequence of Brachyspira murdochii type strain (56-150).Pati A, Sikorski J, Gronow S, Munk C, Lapidus A, Copeland A, Glavina Del Tio T, Nolan M, Lucas S, Chen F, Tice H, Cheng JF, Han C, Detter JC, Bruce D, Tapia R, Goodwin L, Pitluck S, Liolios K, Ivanova N, Mavromatis K, Mikhailova N, Chen A, Palaniappan K, Land M, Hauser L, Chang YJ, Jeffries CD, Spring S, Rohde M, Goker M, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HPStand Genomic Sci10.4056/sigs.8319932010

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4674Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12563)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12563
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
41740Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17895
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
68373Automatically annotated from API CAM
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
86843Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42880.1StrainInfo: A central database for resolving microbial strain identifiers
121192Curators of the CIPCollection of Institut Pasteur (CIP 105832)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105832