Name: Afipia clevelandensis Cleveland clinic foundation strain
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1777

Type strain:

Species: Afipia clevelandensis

Strain Designation: Cleveland clinic foundation strain, 411m, B91-007353, F6703, G1849

Culture col. no.: CCUG 30457, DSM 7315, ATCC 49720, CIP 103516

Strain history: CIP <- 1992, ATCC <- D.J. Brenner, CDC: strain B91-007353 <- G.S. Hall: strain G1849, F6703

NCBI tax ID(s): 883079 (strain), 1034 (species)

SI-ID 12657

LMG 18883, ATCC 49720, CIP 103516, DSM 7315, NCTC 12721, CCUG 30457

Section

Afipia clevelandensis Cleveland clinic foundation strain is a mesophilic, motile bacterium that was isolated from human tibia, biopsy.

motile
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Nitrobacteraceae*
	Genus	*Afipia*
	Species	**Afipia clevelandensis**
	Full Scientific Name (LPSN)	Afipia clevelandensis Brenner et al. 1992

Information on morphological and physiological properties Morphology

[Ref.: #118308]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.973

[Ref.: #118308]	Cell shape	rod-shaped

[Ref.: #118308]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %91.107

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3117]

Culture medium

BCYE-AGAR (DSMZ Medium 585)

[Ref.: #3117]

Culture medium growth

[Ref.: #3117]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3117]

Culture medium composition

expand / minimize

[Ref.: #40686]

Culture medium

MEDIUM 23 - for Afipia and Legionella

[Ref.: #40686]

Culture medium growth

[Ref.: #40686]

Culture medium composition

expand / minimize

[Ref.: #118308]

Culture medium

CIP Medium 23

[Ref.: #118308]

Culture medium growth

[Ref.: #118308]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #3117]		growth	28 °C
[Ref.: #40686]		growth	30 °C
[Ref.: #50091]		growth	37 °C
[Ref.: #118308]		growth	30 °C
[Ref.: #118308]	no	growth	10 °C
[Ref.: #118308]	no	growth	15 °C
[Ref.: #118308]	no	growth	41 °C

[Ref.: #3117]	Temperature range	mesophilic
[Ref.: #40686]	Temperature range	mesophilic
[Ref.: #50091]	Temperature range	mesophilic
[Ref.: #118308]	Temperature range	mesophilic
[Ref.: #118308]	Temperature range	psychrophilic
[Ref.: #118308]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.998

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #118308]	glucose ChEBI	-	degradation
[Ref.: #118308]	malonate ChEBI	-	assimilation
[Ref.: #118308]	nitrate ChEBI	-	reduction
[Ref.: #118308]	nitrate ChEBI	-	respiration
[Ref.: #118308]	nitrite ChEBI	-	reduction

	Enzyme	Enzyme activity	EC number
[Ref.: #118308]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #118308]	catalase	-	1.11.1.6
[Ref.: #118308]	lysine decarboxylase	-	4.1.1.18
[Ref.: #118308]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #118308]	oxidase	+
[Ref.: #118308]	urease	+	3.5.1.5

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #50091]

Fatty acid	Percentage	ECL
C18:1 ω7c /12t/9t	26.6	17.824
C17:0 CYCLO	23.1	16.888
11 methyl 18:1 ω7c	13.3	18.081
C18:0	12.2	18
C16:1 ω7c	9.2	15.819
C19:0 CYCLO ω8c	8.6	18.9
C16:0	5.5	16
C20:1 ω7c	0.9	19.833
C17:0	0.7	17

Metadata FA analysis

Reference

[Ref.: #50091]

Fatty acid	Percentage	ECL
C18:1 ω7c /12t/9t	28.5	17.824
C16:1 ω7c	19.5	15.819
C17:0 CYCLO	16.3	16.888
C18:0	12.4	18
C16:0	7.1	16
11 methyl 18:1 ω7c	6.8	18.081
C18:2 ω6,9c/C18:0 ANTE	3.5	17.724
C19:0 CYCLO ω8c	2.6	18.9
C20:1 ω7c	1.4	19.833
C14:0	0.7	14
Unidentified	0.6	19.785
Unidentified	0.4	18.441
Unidentified	0.3	13.742

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3117]	Sample type/isolated from	human tibia, biopsy
[Ref.: #3117]	Geographic location (country and/or sea, region)	Ohio
[Ref.: #3117]	Country	USA
[Ref.: #3117]	Country ISO 3 Code	USA
[Ref.: #3117]	Continent	North America

[Ref.: #50091]	Sample type/isolated from	Tibial biopsy,male,age 69yr,died
[Ref.: #50091]	Sampling date	1988
[Ref.: #50091]	Geographic location (country and/or sea, region)	Ohio
[Ref.: #50091]	Country	USA
[Ref.: #50091]	Country ISO 3 Code	USA
[Ref.: #50091]	Continent	North America

[Ref.: #118308]	Sample type/isolated from	Human, Tibial biopsy
* marker position based on {}

Isolation sources categories

#Infection	#Patient	#Biopsy
#Host	#Human	-
#Host Body-Site	#Limb	#Leg

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3117]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #118308]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Afipia clevelandensis strain B-91-007353 16S ribosomal RNA gene, partial sequence	M69186	1425	ENA	883079 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Afipia clevelandensis ATCC 49720	GCA_000336555	scaffold	GenBank	883079 tax ID	*
[Ref.: #66792]	Afipia clevelandensis ATCC 49720	883079.3	wgs	PATRIC	883079 tax ID	*
[Ref.: #66792]	Afipia clevelandensis ATCC 49720	2537561906	draft	JGI IMG/M	883079 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Afipia clevelandensis ATCC 49720 ATCC 49720 NCBI: GCA_000336555.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Afipia clevelandensis ATCC 49720 ATCC 49720 NCBI: GCA_000336555.1
[Ref.: #69480]	motile	yes	77.632	no
[Ref.: #69480]	flagellated	no	64.264	no
[Ref.: #69480]	gram-positive	no	98.588	no
[Ref.: #69480]	anaerobic	no	98.451	no
[Ref.: #69480]	aerobic	yes	93.654	no
[Ref.: #69480]	halophile	no	88.041	no
[Ref.: #69480]	spore-forming	no	96.89	no
[Ref.: #69480]	thermophile	no	97.578	no
[Ref.: #69480]	glucose-util	yes	58.797	no
[Ref.: #69480]	glucose-ferment	no	93.057	no

Availability in culture collections External links

[Ref.: #3117]

Culture collection no.

CCUG 30457, DSM 7315, ATCC 49720, CIP 103516

[Ref.: #71424]

SI-ID 12657

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bosea minatitlanensis sp. nov., a strictly aerobic bacterium isolated from an anaerobic digester.	Ouattara AS, Assih EA, Thierry S, Cayol JL, Labat M, Monroy O, Macarie H	Int J Syst Evol Microbiol	10.1099/ijs.0.02540-0	2003	*

References

#3117

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7315

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40686 ; Curators of the CIP;
#50091 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 30457

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71424

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID12657.1 )

#118308 Collection of Institut Pasteur ; Curators of the CIP; CIP 103516
* These data were automatically processed and therefore are not curated

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Afipia clevelandensis ATCC 49720 ATCC 49720

Afipia clevelandensis ATCC 49720 ATCC 49720

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