Name: Afipia felis B-91-007352
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1778

Type strain:

Species: Afipia felis

Strain Designation: B-91-007352, CSD1, CSD 1

Culture col. no.: DSM 7326, ATCC 53690, CCUG 30456, CIP 103515

Strain history: CIP <- 1992, ATCC <- Armed Forces Inst. Path.: strain CSD 1

NCBI tax ID(s): 883080 (strain), 1035 (species)

SI-ID 12658

LMG 18884, ATCC 53690, CIP 103515, DSM 7326, NCTC 12499, CCUG 30456

Special Collections

Other strains from Afipia felis

Section

Afipia felis B-91-007352 is an obligate aerobe, Gram-negative human pathogen that was isolated from human lymph node tissue.

Gram-negative
human pathogen
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Nitrobacteraceae*
	Genus	*Afipia*
	Species	**Afipia felis**
	Full Scientific Name (LPSN)	Afipia felis Brenner et al. 1992

Information on morphological and physiological properties Morphology

[Ref.: #119298]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %98.979

[Ref.: #119298]	Cell shape	rod-shaped

[Ref.: #119298]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3001]

Culture medium

BCYE-AGAR (DSMZ Medium 585)

[Ref.: #3001]

Culture medium growth

[Ref.: #3001]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3001]

Culture medium composition

expand / minimize

[Ref.: #36858]

Culture medium

MEDIUM 23 - for Afipia and Legionella

[Ref.: #36858]

Culture medium growth

[Ref.: #36858]

Culture medium composition

expand / minimize

[Ref.: #119298]

Culture medium

CIP Medium 23

[Ref.: #119298]

Culture medium growth

[Ref.: #119298]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #3001]		growth	30 °C
[Ref.: #36858]		growth	30 °C
[Ref.: #50090]		growth	37 °C
[Ref.: #119298]		growth	22-30 °C
[Ref.: #119298]	no	growth	37 °C
[Ref.: #119298]	no	growth	41 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #119298]

Oxygen tolerance

obligate aerobe

[Ref.: #69481]

Ability of spore formation

no Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #119298]	glucose ChEBI	-	degradation
[Ref.: #119298]	malonate ChEBI	-	assimilation
[Ref.: #119298]	nitrate ChEBI	+	reduction
[Ref.: #119298]	nitrate ChEBI	-	respiration
[Ref.: #119298]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #119298]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #119298]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #119298]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #119298]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #119298]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #119298]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #119298]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119298]	tryptophan deaminase	-
[Ref.: #119298]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #50090]

Fatty acid	Percentage	ECL
C19:0 CYCLO ω8c	24.1	18.9
C18:1 ω7c /12t/9t	21.7	17.824
C17:0 CYCLO	20.5	16.888
C16:0	13.9	16
11 methyl 18:1 ω7c	8.1	18.081
C18:0	5.9	18
C16:1 ω7c	5.7	15.819

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119298]	7561	-	-	+	+	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3001]	Sample type/isolated from	human lymph node tissue
[Ref.: #3001]	Geographic location (country and/or sea, region)	USA
[Ref.: #3001]	Country	USA
[Ref.: #3001]	Country ISO 3 Code	USA
[Ref.: #3001]	Continent	North America

[Ref.: #50090]	Sample type/isolated from	Lymph node,patient with CSD
[Ref.: #50090]	Sampling date	1987
[Ref.: #50090]	Geographic location (country and/or sea, region)	Washington DC
[Ref.: #50090]	Country	USA
[Ref.: #50090]	Country ISO 3 Code	USA
[Ref.: #50090]	Continent	North America

[Ref.: #119298]	Sample type/isolated from	Human, Lymph node tissue
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body-Site	#Organ	#Lymph node

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HF970590 (>99% sequence identity) for Afipia from Microbeatlas

Aquatic Samples	275
Soil Samples	269
Animal Samples	203
Plant Samples	60
Total Samples	807

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3001]	Pathogenicity (human)	yes
[Ref.: #3001]	Pathogenicity (animal)	yes
[Ref.: #3001]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #119298]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Afipia felis ATCC 53690 strain B-91-007352 16S ribosomal RNA gene, partial sequence	M65248	1425	GenBank ENA	883080 tax ID	*
[Ref.: #20218]	Afipia felis partial 16S rRNA gene, strain CIP 103515	HF970590	1411	GenBank ENA	1035 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Afipia felis ATCC 53690	GCA_000314735	scaffold	GenBank ENA	883080 tax ID	*
[Ref.: #66792]	Afipia felis ATCC 53690	883080.3	wgs	PATRIC	883080 tax ID	*
[Ref.: #66792]	Afipia felis felis ATCC 53690	2537561903	draft	JGI IMG/M	883080 tax ID	*

[Ref.: #3001]

GC-content

62.5 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Afipia felis ATCC 53690 ATCC 53690 NCBI: GCA_000314735.2
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Afipia felis ATCC 53690 ATCC 53690 NCBI: GCA_000314735.2
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	98.979	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	92.282	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	76.184	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	86.616	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	97.887	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	70.152	no

Availability in culture collections External links

[Ref.: #3001]

Culture collection no.

DSM 7326, ATCC 53690, CCUG 30456, CIP 103515

[Ref.: #71425]

SI-ID 12658

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Isolation and properties of methanesulfonate-degrading Afipia felis from Antarctica and comparison with other strains of A. felis.	Moosvi SA, Pacheco CC, McDonald IR, De Marco P, Pearce DA, Kelly DP, Wood AP	Environ Microbiol	10.1111/j.1462-2920.2004.00661.x	2005	*
Phylogeny	Bosea minatitlanensis sp. nov., a strictly aerobic bacterium isolated from an anaerobic digester.	Ouattara AS, Assih EA, Thierry S, Cayol JL, Labat M, Monroy O, Macarie H	Int J Syst Evol Microbiol	10.1099/ijs.0.02540-0	2003	*
Enzymology	Production and characterization of mouse monoclonal antibodies against Afipia felis.	Engbaek K, Uttenthal LO, Koch C	APMIS	10.1111/j.1699-0463.1997.tb00558.x	1997	*
Pathogenicity	Diagnostic value of the indirect immunofluorescence assay in cat scratch disease with Bartonella henselae and Afipia felis antigens.	Amerein MP, De Briel D, Jaulhac B, Meyer P, Monteil H, Piemont Y	Clin Diagn Lab Immunol	10.1128/cdli.3.2.200-204.1996	1996	*

References

#3001

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7326

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#36858 ; Curators of the CIP;
#50090 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 30456

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71425

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID12658.1 )

#119298 Collection of Institut Pasteur ; Curators of the CIP; CIP 103515
* These data were automatically processed and therefore are not curated

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Afipia felis ATCC 53690 ATCC 53690

Afipia felis ATCC 53690 ATCC 53690

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