Afipia felis (DSM 7326, ATCC 53690, B-91-007352)

Name: Afipia felis (DSM 7326, ATCC 53690, B-91-007352)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1778

Type strain

Strain Designation B-91-007352, CSD1, CSD 1

Culture col. no. DSM 7326 ATCC 53690 B-91-007352 CSD1 CCUG 30456 2 more

NCBI tax ID(s) 883080 1035

Special Collections DSMZ CCUG CIP

Links

Afipia felis B-91-007352 is an obligate aerobe, mesophilic, Gram-negative human pathogen that was isolated from human lymph node tissue.

Gram-negative motile rod-shaped obligate aerobe mesophilic human pathogen genome sequence 16S sequence

Name and taxonomic classification

SI-ID 12658

LMG 18884,ATCC 53690,CIP 103515,DSM 7326,NCTC 12499,CCUG 30456

@ref 20215

Last LPSN update 2025-12-16 09:47:11

Domain Pseudomonadati

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Hyphomicrobiales

Family Nitrobacteraceae

Genus Afipia

Species Afipia felis

Full scientific name Afipia felis Brenner et al. 1992

BacDive ID	Other strains from **Afipia felis** (2)	Type strain
1779	A. felis 25E-1, DSM 16694
135486	A. felis 76713, CIP 106335, CCUG 43109

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
119298	negative	rod-shaped
125438	negative		98.979
125439	negative		99.9

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
3001	BCYE-AGAR (DSMZ Medium 585)	Medium recipe at MediaDive	Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
36858	MEDIUM 23 - for Afipia and Legionella		Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
119298	CIP Medium 23	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
3001	positive	growth	30	mesophilic
36858	positive	growth	30	mesophilic
50090	positive	growth	37	mesophilic
119298	positive	growth	22-30
119298	negative	growth	37
119298	negative	growth	41

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
119298	obligate aerobe
125439	obligate aerobe	96.4

Spore formation

@ref	Spore formation	Confidence
125439		99.6

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
119298	17234 ChEBI	glucose	-	degradation
119298	15792 ChEBI	malonate	-	assimilation
119298	17632 ChEBI	nitrate	+	reduction
119298	17632 ChEBI	nitrate	-	respiration
119298	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
119298	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
119298	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
119298	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
119298	gelatinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
119298	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
119298	ornithine decarboxylase	-	4.1.1.17
119298	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
119298	tryptophan deaminase	-
119298	urease	+	3.5.1.5
68382	valine arylamidase	-		from API zym

Fatty acid profile

Metadata FA analysis

@ref

50090

Fatty acid	Percentage	ECL
C19:0 CYCLO ω8c	24.1	18.9
C18:1 ω7c /12t/9t	21.7	17.824
C17:0 CYCLO	20.5	16.888
C16:0	13.9	16
11 methyl 18:1 ω7c	8.1	18.081
C18:0	5.9	18
C16:1 ω7c	5.7	15.819

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119298	-	-	+	+	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Human	-
#Host Body-Site	#Organ	#Lymph node

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent
3001	human lymph node tissue		USA	USA	USA	North America
50090	Lymph node,patient with CSD	1987	Washington DC	USA	USA	North America
119298	Human, Lymph node tissue

Taxonmaps

69479

Global distribution of 16S sequence HF970590 (>99% sequence identity) for Afipia from Microbeatlas

Aquatic Samples	275
Soil Samples	269
Animal Samples	203
Plant Samples	60
Total Samples	807

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Pathogenicity animal	Biosafety level	Biosafety level comment
3001			2	Risk group (German classification) According to TRBA 466
119298			1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	Afip_feli_felis_ATCC_53690_V1 assembly for Afipia felis ATCC 53690	scaffold	GCA_000314735	883080.3	2537561903	883080	64.25

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Afipia felis ATCC 53690 strain B-91-007352 16S ribosomal RNA gene, partial sequence	M65248	1425		883080
20218	Afipia felis partial 16S rRNA gene, strain CIP 103515	HF970590	1411		1035

GC content

@ref	GC-content (mol%)	Method
3001	62.5	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Afipia felis ATCC 53690
NCBI: GCA_000314735

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.60	no
125439	motility	BacteriaNetⓘ	yes	85.30	no
125439	gram_stain	BacteriaNetⓘ	negative	99.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	96.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Afipia felis ATCC 53690 ATCC 53690
NCBI: GCA_000314735.2

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.98	no
125438	anaerobic	anaerobicⓘ	no	92.28	no
125438	aerobic	aerobicⓘ	yes	76.18	no
125438	spore-forming	spore-formingⓘ	no	86.62	no
125438	thermophilic	thermophileⓘ	no	97.89	yes
125438	flagellated	motile2+ⓘ	yes	70.15	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Afipia felis induces uptake by macrophages directly into a nonendocytic compartment.	Luhrmann A, Streker K, Schuttfort A, Daniels JJ, Haas A.	Proc Natl Acad Sci U S A	10.1073/pnas.121190398	2001
Phylogeny	Reclassification of Pseudomonas boreopolis Gray and Thornton 1928 (Approved Lists 1980) as Xanthomonas boreopolis comb. nov. and Pseudomonas carboxydohydrogena (ex Sanjieva and Zavarzin 1971) Meyer et al. 1980 as Afipia carboxydohydrogena comb. nov. based on comparative genomic analysis.	Fatahi-Bafghi M.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006865	2025
Genetics	Metagenomic survey of methanesulfonic acid (MSA) catabolic genes in an Atlantic Ocean surface water sample and in a partial enrichment.	Henriques AC, Azevedo RM, De Marco P.	PeerJ	10.7717/peerj.2498	2016
Enzymology	Highly Sensitive Bacteriophage-Based Detection of Brucella abortus in Mixed Culture and Spiked Blood.	Sergueev KV, Filippov AA, Nikolich MP.	Viruses	10.3390/v9060144	2017
Genetics	Evidence of carbon fixation pathway in a bacterium from candidate phylum SBR1093 revealed with genomic analysis.	Wang Z, Guo F, Liu L, Zhang T.	PLoS One	10.1371/journal.pone.0109571	2014
	Legionella-like and other amoebal pathogens as agents of community-acquired pneumonia.	Marrie TJ, Raoult D, La Scola B, Birtles RJ, de Carolis E, Canadian Community-Acquired Pneumonia Study Group.	Emerg Infect Dis	10.3201/eid0706.010619	2001
	Isoprenoid quinones of "Afipia" spp.	Moss CW, Daneshvar MI, Hollis DG, Birkness KA.	J Clin Microbiol	10.1128/jcm.29.12.2904-2905.1991	1991
Enzymology	A case of Q fever prosthetic joint infection and description of an assay for detection of Coxiella burnetii.	Tande AJ, Cunningham SA, Raoult D, Sim FH, Berbari EF, Patel R.	J Clin Microbiol	10.1128/jcm.02352-12	2013
Phylogeny	Usefulness of rpoB gene sequencing for identification of Afipia and Bosea species, including a strategy for choosing discriminative partial sequences.	Khamis A, Colson P, Raoult D, Scola BL.	Appl Environ Microbiol	10.1128/aem.69.11.6740-6749.2003	2003
	Cat scratch disease: detection of Bartonella henselae DNA in archival biopsies from patients with clinically, serologically, and histologically defined disease.	Scott MA, McCurley TL, Vnencak-Jones CL, Hager C, McCoy JA, Anderson B, Collins RD, Edwards KM.	Am J Pathol		1996
Enzymology	Semiquantitative species-specific detection of Bartonella henselae and Bartonella quintana by PCR-enzyme immunoassay.	Sander A, Penno S.	J Clin Microbiol	10.1128/jcm.37.10.3097-3101.1999	1999
Enzymology	Detection of Rochalimaea henselae DNA in specimens from cat scratch disease patients by PCR.	Anderson B, Sims K, Regnery R, Robinson L, Schmidt MJ, Goral S, Hager C, Edwards K.	J Clin Microbiol	10.1128/jcm.32.4.942-948.1994	1994
Enzymology	Differentiation of Bartonella-like isolates at the species level by PCR-restriction fragment length polymorphism in the citrate synthase gene.	Norman AF, Regnery R, Jameson P, Greene C, Krause DC.	J Clin Microbiol	10.1128/jcm.33.7.1797-1803.1995	1995
	Compilation of small ribosomal subunit RNA structures.	Neefs JM, Van de Peer Y, De Rijk P, Chapelle S, De Wachter R.	Nucleic Acids Res	10.1093/nar/21.13.3025	1993
Phylogeny	Phylogenetic placement and characterization of a new alpha-2 proteobacterium isolated from a patient with sepsis.	Blomqvist G, Wesslen L, Pahlson C, Hjelm E, Pettersson B, Nikkila T, Allard U, Svensson O, Uhlen M, Morein B, Friman G.	J Clin Microbiol	10.1128/jcm.35.8.1988-1995.1997	1997
Phylogeny	Isolation and properties of methanesulfonate-degrading Afipia felis from Antarctica and comparison with other strains of A. felis.	Moosvi SA, Pacheco CC, McDonald IR, De Marco P, Pearce DA, Kelly DP, Wood AP	Environ Microbiol	10.1111/j.1462-2920.2004.00661.x	2005
Enzymology	Production and characterization of mouse monoclonal antibodies against Afipia felis.	Engbaek K, Uttenthal LO, Koch C	APMIS	10.1111/j.1699-0463.1997.tb00558.x	1997
Pathogenicity	Diagnostic value of the indirect immunofluorescence assay in cat scratch disease with Bartonella henselae and Afipia felis antigens.	Amerein MP, De Briel D, Jaulhac B, Meyer P, Monteil H, Piemont Y	Clin Diagn Lab Immunol	10.1128/cdli.3.2.200-204.1996	1996
Phylogeny	Proposal of Afipia gen. nov., with Afipia felis sp. nov. (formerly the cat scratch disease bacillus), Afipia clevelandensis sp. nov. (formerly the Cleveland Clinic Foundation strain), Afipia broomeae sp. nov., and three unnamed genospecies.	Brenner DJ, Hollis DG, Moss CW, English CK, Hall GS, Vincent J, Radosevic J, Birkness KA, Bibb WF, Quinn FD.	J Clin Microbiol	10.1128/jcm.29.11.2450-2460.1991	1991
Phylogeny	Bosea minatitlanensis sp. nov., a strictly aerobic bacterium isolated from an anaerobic digester.	Ouattara AS, Assih EA, Thierry S, Cayol JL, Labat M, Monroy O, Macarie H	Int J Syst Evol Microbiol	10.1099/ijs.0.02540-0	2003

References

#3001	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7326
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#36858	; Curators of the CIP;
#50090	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 30456
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119298	Collection of Institut Pasteur ; Curators of the CIP; CIP 103515
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Afipia felis (DSM 7326, ATCC 53690, B-91-007352)

Name and taxonomic classification