Name: Mitsuokella multacida A 405-1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 17134

Type strain:

Species: Mitsuokella multacida

Strain Designation: A 405-1

Culture col. no.: DSM 20544, ATCC 27723, NCTC 10934, CCUG 21055, CCM 6003, JCM 2054, CIP 107116

Strain history: CIP <- 2001, CCM <- T. Mitsuoka: strain A 405-1

NCBI tax ID(s): 500635 (strain), 52226 (species)

SI-ID 47010

ATCC 27723, NCTC 10934, CCUG 21055, CCM 6003, DSM 20544, JCM 2054, CIP 107116

Special Collections

Other strains from Mitsuokella multacida

Section

Mitsuokella multacida A 405-1 is an anaerobe bacterium that was isolated from human faeces.

anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Negativicutes*
	Order	*Selenomonadales*
	Family	*Selenomonadaceae*
	Genus	*Mitsuokella*
	Species	**Mitsuokella multacida**
	Full Scientific Name (LPSN)	Mitsuokella multacida corrig. (Mitsuoka et al. 1974) Shah and Collins 1983

	Synonym	Bacteroides multiacidus
	Synonym	Mitsuokella multiacidus

Information on morphological and physiological properties Morphology

[Ref.: #121172]	Gram stain	negative

[Ref.: #121172]	Cell shape	rod-shaped

[Ref.: #121172]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8773]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #8773]

Culture medium growth

[Ref.: #8773]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8773]

Culture medium composition

expand / minimize

[Ref.: #39892]

Culture medium

MEDIUM 137 - for Ruminococcus hansenii and Rarobacter incanus

[Ref.: #39892]

Culture medium growth

[Ref.: #39892]

Culture medium composition

expand / minimize

[Ref.: #8773]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #8773]

Culture medium growth

[Ref.: #8773]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #8773]

Culture medium

FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a)

[Ref.: #8773]

Culture medium growth

[Ref.: #8773]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #121172]

Culture medium

CIP Medium 137

[Ref.: #121172]

Culture medium growth

[Ref.: #121172]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #8773]	growth	37 °C
[Ref.: #39892]	growth	37 °C
[Ref.: #67770]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8773]	Oxygen tolerance	anaerobe
[Ref.: #121172]	Oxygen tolerance	anaerobe

[Ref.: #69481]

Ability of spore formation

no Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #121172]	cellobiose ChEBI	+/-	degradation
[Ref.: #121172]	D-arabinose ChEBI	+/-	degradation
[Ref.: #121172]	D-fructose ChEBI	+/-	degradation
[Ref.: #121172]	D-glucose ChEBI	+/-	degradation
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #121172]	D-xylose ChEBI	+/-	degradation
[Ref.: #121172]	esculin ChEBI	+	hydrolysis
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #121172]	lactose ChEBI	+/-	degradation
[Ref.: #121172]	maltose ChEBI	+/-	degradation
[Ref.: #121172]	nitrate ChEBI	+	reduction
[Ref.: #121172]	nitrate ChEBI	+	respiration
[Ref.: #68380]	nitrate ChEBI	+	reduction	* from API rID32A
[Ref.: #121172]	nitrite ChEBI	-	reduction
[Ref.: #68380]	raffinose ChEBI	+	fermentation	* from API rID32A
[Ref.: #121172]	salicin ChEBI	+/-	degradation
[Ref.: #121172]	sucrose ChEBI	+/-	degradation
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #121172]		indole ChEBI	no
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A, API zym
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A, API zym
[Ref.: #68380]	alpha-galactosidase	+	3.2.1.22	* from API rID32A, API zym
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121172]	amylase	-
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #121172]	beta-galactosidase	+	3.2.1.23
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A, API zym
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	+	3.2.1.21	* from API rID32A, API zym
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A, API zym
[Ref.: #121172]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #121172]	DNase	-
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #121172]	gelatinase	-
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #121172]	lecithinase	-
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A, API zym
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #121172]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121172]	oxidase	-
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #121172]	protease	-
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #121172]	tween esterase	-
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #121172]	urease	-	3.5.1.5
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #8773]	13344	-	-	+	+	-	+	+	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121172]	10078	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	+	+	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8773]	Sample type/isolated from	human faeces

[Ref.: #47265]	Sample type/isolated from	Human feces

[Ref.: #67770]	Sample type/isolated from	Human feces

[Ref.: #121172]	Sample type/isolated from	Human, Feces
[Ref.: #121172]	Country	Japan
[Ref.: #121172]	Country ISO 3 Code	JPN
[Ref.: #121172]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC036321 (>99% sequence identity) for Mitsuokella multacida subclade from Microbeatlas

Aquatic Samples	684
Soil Samples	103
Animal Samples	11825
Plant Samples	122
Total Samples	12734

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8773]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121172]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8773]	M.multiacidus 16S rRNA gene	X81878	1511	GenBank ENA	500635 tax ID
[Ref.: #67770]	Mitsuokella multacida gene for 16S ribosomal RNA, partial sequence, strain: JCM 2054	LC036321	1522	GenBank ENA	52226 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Mitsuokella multacida DSM 20544	GCA_000155955	scaffold	GenBank ENA	500635 tax ID
[Ref.: #66792]	Mitsuokella multacida DSM 20544	500635.8	wgs	PATRIC	500635 tax ID	*
[Ref.: #66792]	Mitsuokella multacida DSM 20544	2562617158	draft	JGI IMG/M	500635 tax ID	*

[Ref.: #8773]	GC-content	57.3 mol%
[Ref.: #67770]	GC-content	57.9 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Mitsuokella multacida DSM 20544 DSM 20544 NCBI: GCA_000155955.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mitsuokella multacida DSM 20544 PATRIC: 500635.8
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	87.679	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	89.512	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	76.559	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	91.727	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	93.583	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	78.059	no

Availability in culture collections External links

[Ref.: #8773]

Culture collection no.

DSM 20544, ATCC 27723, NCTC 10934, CCUG 21055, CCM 6003, JCM 2054, CIP 107116

[Ref.: #86133]

SI-ID 47010

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Mitsuokella jalaludinii sp. nov., from the rumens of cattle in Malaysia.	Lan GQ, Ho YW, Abdullah N	Int J Syst Evol Microbiol	10.1099/00207713-52-3-713	2002	*

References

#8773

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20544

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#39892 ; Curators of the CIP;
#47265 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 21055

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68380

Automatically annotated from API rID32A .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#86133

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID47010.1 )

#121172 Collection of Institut Pasteur ; Curators of the CIP; CIP 107116
* These data were automatically processed and therefore are not curated

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Mitsuokella multacida DSM 20544 DSM 20544

Mitsuokella multacida DSM 20544

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