Name: Megasphaera micronuciformis DSM 17226
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17133

Type strain:

Species: Megasphaera micronuciformis

Culture col. no.: DSM 17226, CCUG 45952, CIP 107280, AIP 412.00

Strain history: CIP <- 2001, J.P. Carlier, Inst. Pasteur, Paris, France: strain AIP 412.00 <- 2000, Montpellier Hosp., Montpellier, France

NCBI tax ID(s): 187326 (species)

SI-ID 87921

CIP 107280, CCUG 45952, DSM 17226, VTT E-042113

Section

Megasphaera micronuciformis DSM 17226 is an anaerobe, mesophilic, Gram-negative human pathogen that was isolated from human, liver abscess.

Gram-negative
rod-shaped
anaerobe
human pathogen
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Negativicutes*
	Order	*Veillonellales*
	Family	*Veillonellaceae*
	Genus	*Megasphaera*
	Species	**Megasphaera micronuciformis**
	Full Scientific Name (LPSN)	Megasphaera micronuciformis Marchandin et al. 2003

Information on morphological and physiological properties Morphology

[Ref.: #120949]	Gram stain	negative

[Ref.: #120949]	Cell shape	rod-shaped

[Ref.: #120949]	Motility	no

[Ref.: #6825]	Incubation period	2-3 days

[Ref.: #56999]	Incubation period	2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6825]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #6825]

Culture medium growth

[Ref.: #6825]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6825]

Culture medium composition

expand / minimize

[Ref.: #38195]

Culture medium

MEDIUM 20 - for Anaerobic bacteria

[Ref.: #38195]

Culture medium growth

[Ref.: #38195]

Culture medium composition

expand / minimize

[Ref.: #6825]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #6825]

Culture medium growth

[Ref.: #6825]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6825]

Culture medium composition

expand / minimize

[Ref.: #6825]

Culture medium

MRS pre-reduced (DSMZ Medium 11b)

[Ref.: #6825]

Culture medium growth

[Ref.: #6825]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6825]

Culture medium composition

expand / minimize

[Ref.: #120949]

Culture medium

CIP Medium 20

[Ref.: #120949]

Culture medium growth

[Ref.: #120949]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #6825]	growth	37 °C
[Ref.: #38195]	growth	37 °C
[Ref.: #56999]	growth	37 °C
[Ref.: #120949]	growth	37 °C

[Ref.: #6825]	Temperature range	mesophilic
[Ref.: #38195]	Temperature range	mesophilic
[Ref.: #56999]	Temperature range	mesophilic
[Ref.: #120949]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #6825]	Oxygen tolerance	anaerobe
[Ref.: #56999]	Oxygen tolerance	anaerobe
[Ref.: #120949]	Oxygen tolerance	anaerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #120949]	nitrate ChEBI	-	reduction
[Ref.: #120949]	nitrate ChEBI	+	respiration
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #120949]	nitrite ChEBI	-	reduction
[Ref.: #68380]	raffinose ChEBI	+	fermentation	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	+	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #120949]	beta-galactosidase	-	3.2.1.23
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #120949]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	+		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	+		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	+	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #120949]	urease	-	3.5.1.5
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #6825]	12201	-	-	+	+	-	+	+/-	-	-	-	-	+	-	-	-	-	-	+	+	-	-	-	-	-	+	+	-	-	+/-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6825]	Sample type/isolated from	human, liver abscess
[Ref.: #6825]	Geographic location (country and/or sea, region)	Paris
[Ref.: #6825]	Country	France
[Ref.: #6825]	Country ISO 3 Code	FRA
[Ref.: #6825]	Continent	Europe

[Ref.: #56999]	Sample type/isolated from	Human liver abscess
[Ref.: #56999]	Geographic location (country and/or sea, region)	Montpellier,CHU Arnaud de V.
[Ref.: #56999]	Country	France
[Ref.: #56999]	Country ISO 3 Code	FRA
[Ref.: #56999]	Continent	Europe

[Ref.: #120949]	Sample type/isolated from	Human, Liver abscess
[Ref.: #120949]	Geographic location (country and/or sea, region)	Montpellier
[Ref.: #120949]	Country	France
[Ref.: #120949]	Country ISO 3 Code	FRA
[Ref.: #120949]	Continent	Europe
[Ref.: #120949]	Isolation date	2000
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body-Site	#Other	#Abscess
#Host Body-Site	#Organ	#Liver

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF473834 (>99% sequence identity) for Megasphaera micronuciformis subclade from Microbeatlas

Aquatic Samples	1197
Soil Samples	673
Animal Samples	71231
Plant Samples	386
Total Samples	73487

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6825]	Pathogenicity (human)	yes, in single cases
[Ref.: #6825]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120949]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6825]	Megasphaera micronuciformis strain AIP 49.01 16S ribosomal RNA gene, partial sequence	AF473833	1416	ENA	187326 tax ID
[Ref.: #20218]	Megasphaera micronuciformis gene for 16S rRNA, partial sequence, strain: DSM 17226	AB609707	1180	ENA	187326 tax ID	*
[Ref.: #20218]	Megasphaera micronuciformis strain AIP 412.00 16S ribosomal RNA gene, partial sequence	AF473834	1411	ENA	187326 tax ID	*

[Ref.: #6825]

GC-content

46.4 mol%

Availability in culture collections External links

[Ref.: #6825]

Culture collection no.

DSM 17226, CCUG 45952, CIP 107280, AIP 412.00

[Ref.: #86132]

SI-ID 87921

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Phylogenetic analysis of some Sporomusa sub-branch members isolated from human clinical specimens: description of Megasphaera micronuciformis sp. nov.	Marchandin H, Jumas-Bilak E, Gay B, Teyssier C, Jean-Pierre H, Simeon de Buochberg M, Carriere C, Carlier JP	Int J Syst Evol Microbiol	10.1099/ijs.0.02378-0	2003	*

References

#6825

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17226

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#38195 ; Curators of the CIP;
#56999 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 45952

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#86132

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID87921.1 )

#120949 Collection of Institut Pasteur ; Curators of the CIP; CIP 107280
* These data were automatically processed and therefore are not curated

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