Name: Megasphaera sueciensis DSM 17042
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17132

Type strain:

Species: Megasphaera sueciensis

Culture col. no.: DSM 17042, VTT E-97791

Strain history: <- R. Juvonen <- VTT

NCBI tax ID(s): 349094 (species)

SI-ID 290986

DSM 17042, VTT E-97791

Section

Megasphaera sueciensis DSM 17042 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from spoiled Swedish beer.

coccus-shaped
Gram-negative
anaerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Negativicutes*
	Order	*Veillonellales*
	Family	*Veillonellaceae*
	Genus	*Megasphaera*
	Species	**Megasphaera sueciensis**
	Full Scientific Name (LPSN)	Megasphaera sueciensis Juvonen and Suihko 2006

Information on morphological and physiological properties Morphology

[Ref.: #31505]	Gram stain	negative

[Ref.: #31505]	Cell length	1-1.4 µm

[Ref.: #31505]	Cell width	0.8-1.2 µm

[Ref.: #31505]	Cell shape	coccus-shaped

[Ref.: #31505]	Motility	no

[Ref.: #31505]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6703]

Culture medium

FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203)

[Ref.: #6703]

Culture medium growth

[Ref.: #6703]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6703]

Culture medium composition

expand / minimize

[Ref.: #6703]

Culture medium

MRS pre-reduced (DSMZ Medium 11b)

[Ref.: #6703]

Culture medium growth

[Ref.: #6703]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6703]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #6703]	growth	30 °C
[Ref.: #31505]	growth	15-37 °C
[Ref.: #31505]	optimum	30 °C

[Ref.: #6703]	Temperature range	mesophilic
[Ref.: #31505]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31505]			optimum	7
[Ref.: #31505]			growth	7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #6703]	Oxygen tolerance	anaerobe
[Ref.: #31505]	Oxygen tolerance	anaerobe

[Ref.: #31505]

Ability of spore formation

[Ref.: #31505]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #31505]	gluconate ChEBI	+	carbon source
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #31505]	pyruvate ChEBI	+	carbon source
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	-	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	-	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #6703]	12190	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6703]	Sample type/isolated from	spoiled Swedish beer
[Ref.: #6703]	Country	Sweden
[Ref.: #6703]	Country ISO 3 Code	SWE
[Ref.: #6703]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Fermented
#Environmental	#Microbial community	-
#Engineered	#Food production	#Beverage

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ223729 (>99% sequence identity) for Megasphaera from Microbeatlas

Aquatic Samples	1110
Soil Samples	125
Animal Samples	838
Plant Samples	34
Total Samples	2107

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6703]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6703]	Megasphaera sueciensis strain VTT E-97791 16S ribosomal RNA gene, partial sequence	DQ223729	1540	ENA	349094 tax ID
[Ref.: #20218]	Megasphaera sueciensis gene for 16S rRNA, partial sequence, strain: DSM 17042	AB609709	1182	ENA	349094 tax ID	*

[Ref.: #6703]

GC-content

43.1 mol%

Availability in culture collections External links

[Ref.: #6703]

Culture collection no.

DSM 17042, VTT E-97791

[Ref.: #86131]

SI-ID 290986

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Megasphaera paucivorans sp. nov., Megasphaera sueciensis sp. nov. and Pectinatus haikarae sp. nov., isolated from brewery samples, and emended description of the genus Pectinatus.	Juvonen R, Suihko ML	Int J Syst Evol Microbiol	10.1099/ijs.0.63699-0	2006	*

References

#6703

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17042

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#31505

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27803 (see below)

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#86131

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID290986.1 )

#27803

IJSEM 695 2006 ( DOI 10.1099/ijs.0.63699-0 , PubMed 16585679 )

* These data were automatically processed and therefore are not curated

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