Strain identifier

BacDive ID: 17132

Type strain: Yes

Species: Megasphaera sueciensis

Strain history: <- R. Juvonen <- VTT

NCBI tax ID(s): 349094 (species)

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General

@ref: 6703

BacDive-ID: 17132

DSM-Number: 17042

keywords: 16S sequence, Bacteria, anaerobe, Gram-negative, coccus-shaped

description: Megasphaera sueciensis DSM 17042 is an anaerobe, Gram-negative, coccus-shaped bacterium that was isolated from spoiled Swedish beer.

NCBI tax id

  • NCBI tax id: 349094
  • Matching level: species

strain history

  • @ref: 6703
  • history: <- R. Juvonen <- VTT

doi: 10.13145/bacdive17132.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Negativicutes
  • order: Veillonellales
  • family: Veillonellaceae
  • genus: Megasphaera
  • species: Megasphaera sueciensis
  • full scientific name: Megasphaera sueciensis Juvonen and Suihko 2006

@ref: 6703

domain: Bacteria

phylum: Firmicutes

class: Negativicutes

order: Selenomonadales

family: Veillonellaceae

genus: Megasphaera

species: Megasphaera sueciensis

full scientific name: Megasphaera sueciensis Juvonen and Suihko 2006

type strain: yes

Morphology

cell morphology

  • @ref: 31505
  • gram stain: negative
  • cell length: 1-1.4 µm
  • cell width: 0.8-1.2 µm
  • cell shape: coccus-shaped
  • motility: no

pigmentation

  • @ref: 31505
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6703FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203)yeshttps://mediadive.dsmz.de/medium/1203Name: FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203) Composition: Horse blood 100.0 g/l Fastidious Anaerobe Agar 45.7 g/l Distilled water
6703MRS pre-reduced (DSMZ Medium 11b)yeshttps://mediadive.dsmz.de/medium/11bName: MRS MEDIUM (pre-reduced) (DSMZ Medium 11b) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Resazurin 0.001 g/l Distilled water

culture temp

@refgrowthtypetemperature
6703positivegrowth30
31505positivegrowth15-37
31505positiveoptimum30

culture pH

@refabilitytypepH
31505positivegrowth7
31505positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
6703anaerobe
31505anaerobe

spore formation

  • @ref: 31505
  • spore formation: no

observation

  • @ref: 31505
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3150524265gluconate+carbon source
3150515361pyruvate+carbon source
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
6703-----------------------------

Isolation, sampling and environmental information

isolation

  • @ref: 6703
  • sample type: spoiled Swedish beer
  • country: Sweden
  • origin.country: SWE
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Fermented
#Environmental#Microbial community
#Engineered#Food production#Beverage

taxonmaps

  • @ref: 69479
  • File name: preview.99_69741.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_37;96_26665;97_37764;98_48985;99_69741&stattab=map
  • Last taxonomy: Megasphaera
  • 16S sequence: DQ223729
  • Sequence Identity:
  • Total samples: 2107
  • soil counts: 125
  • aquatic counts: 1110
  • animal counts: 838
  • plant counts: 34

Safety information

risk assessment

  • @ref: 6703
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Megasphaera sueciensis gene for 16S rRNA, partial sequence, strain: DSM 17042AB6097091182nuccore349094
6703Megasphaera sueciensis strain VTT E-97791 16S ribosomal RNA gene, partial sequenceDQ2237291540nuccore349094

GC content

  • @ref: 6703
  • GC-content: 43.1

External links

@ref: 6703

culture collection no.: DSM 17042, VTT E-97791

straininfo link

  • @ref: 86131
  • straininfo: 290986

literature

  • topic: Phylogeny
  • Pubmed-ID: 16585679
  • title: Megasphaera paucivorans sp. nov., Megasphaera sueciensis sp. nov. and Pectinatus haikarae sp. nov., isolated from brewery samples, and emended description of the genus Pectinatus.
  • authors: Juvonen R, Suihko ML
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.63699-0
  • year: 2006
  • mesh: Beer/*microbiology, Megasphaera/*classification/genetics/isolation & purification/*metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Pectinatus/*classification, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/analysis/genetics
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
6703Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17042)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17042
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
31505Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604127803
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
86131Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID290986.1StrainInfo: A central database for resolving microbial strain identifiers