Tsukamurella inchonensis D-771 is an obligate aerobe, mesophilic, Gram-positive prokaryote that was isolated from blood culture of a patient, who ingested HCl.
Gram-positive rod-shaped obligate aerobe mesophilic 16S sequence| @ref 20215 |
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| Domain Bacillati |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Mycobacteriales |
| Family Tsukamurellaceae |
| Genus Tsukamurella |
| Species Tsukamurella inchonensis |
| Full scientific name Tsukamurella inchonensis Yassin et al. 1995 |
| BacDive ID | Other strains from Tsukamurella inchonensis (1) | Type strain |
|---|---|---|
| 17098 | T. inchonensis IMK-9, DSM 43246, ATCC 25938, DSM 43344, ... |
| @ref | Gram stain | Cell shape | Motility | |
|---|---|---|---|---|
| 120074 | positive | rod-shaped |
| @ref: | 11467 |
| multimedia content: | DSM_44067.jpg |
| multimedia.multimedia content: | https://www.dsmz.de/microorganisms/photos/DSM_44067.jpg |
| caption: | Medium 215 28°C |
| intellectual property rights: | © Leibniz-Institut DSMZ |
| manual_annotation: | 1 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 11467 | BHI MEDIUM (DSMZ Medium 215) | Medium recipe at MediaDive | Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water | ||
| 11467 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | Medium recipe at MediaDive | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water | ||
| 11467 | MIDDLEBROOK MEDIUM (DSMZ Medium 645) | Medium recipe at MediaDive | Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water | ||
| 19624 | ISP 2 | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |||
| 19624 | ISP 3 | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |||
| 19624 | ISP 4 | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |||
| 19624 | ISP 5 | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |||
| 19624 | ISP 6 | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 19624 | ISP 7 | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 39097 | MEDIUM 29- Brain heart agar | Distilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g) | |||
| 120074 | CIP Medium 29 | Medium recipe at CIP |
| 120074 | Oxygen toleranceobligate aerobe |
| 67770 | Observationquinones: MK-9, MK-10, MK-8 |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68368 | 29016 ChEBI | arginine | - | hydrolysis | from API 20E |
| 120074 | 16947 ChEBI | citrate | - | carbon source | |
| 68368 | 16947 ChEBI | citrate | - | assimilation | from API 20E |
| 68379 | 17634 ChEBI | D-glucose | - | fermentation | from API Coryne |
| 68379 | 16899 ChEBI | D-mannitol | - | fermentation | from API Coryne |
| 68379 | 16988 ChEBI | D-ribose | - | fermentation | from API Coryne |
| 68379 | 65327 ChEBI | D-xylose | - | fermentation | from API Coryne |
| 68379 | 4853 ChEBI | esculin | + | hydrolysis | from API Coryne |
| 120074 | 4853 ChEBI | esculin | + | hydrolysis | |
| 68379 | 5291 ChEBI | gelatin | - | hydrolysis | from API Coryne |
| 68368 | 5291 ChEBI | gelatin | - | hydrolysis | from API 20E |
| 68379 | 28087 ChEBI | glycogen | - | fermentation | from API Coryne |
| 120074 | 606565 ChEBI | hippurate | - | hydrolysis | |
| 68379 | 17716 ChEBI | lactose | - | fermentation | from API Coryne |
| 68368 | 25094 ChEBI | lysine | - | degradation | from API 20E |
| 68379 | 17306 ChEBI | maltose | - | fermentation | from API Coryne |
| 68379 | 17632 ChEBI | nitrate | - | reduction | from API Coryne |
| 120074 | 17632 ChEBI | nitrate | - | reduction | |
| 120074 | 17632 ChEBI | nitrate | - | respiration | |
| 120074 | 16301 ChEBI | nitrite | - | reduction | |
| 68368 | 18257 ChEBI | ornithine | - | degradation | from API 20E |
| 68379 | 17992 ChEBI | sucrose | - | fermentation | from API Coryne |
| 68368 | 27897 ChEBI | tryptophan | - | energy source | from API 20E |
| 68379 | 16199 ChEBI | urea | - | hydrolysis | from API Coryne |
| 68368 | 16199 ChEBI | urea | - | hydrolysis | from API 20E |
| @ref | Metabolite | Is antibiotic | Is sensitive | Is resistant | |
|---|---|---|---|---|---|
| 120074 | 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate) |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 120074 | alcohol dehydrogenase | - | 1.1.1.1 | |
| 68382 | alkaline phosphatase | + | 3.1.3.1 | from API zym |
| 68379 | alkaline phosphatase | + | 3.1.3.1 | from API Coryne |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | + | 3.2.1.20 | from API zym |
| 68379 | alpha-glucosidase | + | 3.2.1.20 | from API Coryne |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 120074 | amylase | + | ||
| 68368 | arginine dihydrolase | - | 3.5.3.6 | from API 20E |
| 68382 | beta-galactosidase | + | 3.2.1.23 | from API zym |
| 120074 | beta-galactosidase | + | 3.2.1.23 | |
| 68379 | beta-galactosidase | + | 3.2.1.23 | from API Coryne |
| 68368 | beta-galactosidase | - | 3.2.1.23 | from API 20E |
| 68382 | beta-glucosidase | + | 3.2.1.21 | from API zym |
| 68379 | beta-glucosidase | + | 3.2.1.21 | from API Coryne |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 68379 | beta-glucuronidase | - | 3.2.1.31 | from API Coryne |
| 120074 | caseinase | - | 3.4.21.50 | |
| 120074 | catalase | + | 1.11.1.6 | |
| 120074 | DNase | + | ||
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 120074 | gamma-glutamyltransferase | - | 2.3.2.2 | |
| 120074 | gelatinase | - | ||
| 68379 | gelatinase | - | from API Coryne | |
| 68368 | gelatinase | - | from API 20E | |
| 120074 | lecithinase | - | ||
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 120074 | lipase | - | ||
| 120074 | lysine decarboxylase | - | 4.1.1.18 | |
| 68368 | lysine decarboxylase | - | 4.1.1.18 | from API 20E |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API Coryne |
| 120074 | ornithine decarboxylase | - | 4.1.1.17 | |
| 68368 | ornithine decarboxylase | - | 4.1.1.17 | from API 20E |
| 120074 | oxidase | - | ||
| 120074 | phenylalanine ammonia-lyase | - | 4.3.1.24 | |
| 120074 | protease | - | ||
| 68379 | pyrazinamidase | - | 3.5.1.B15 | from API Coryne |
| 68379 | pyrrolidonyl arylamidase | + | 3.4.19.3 | from API Coryne |
| 68382 | trypsin | - | 3.4.21.4 | from API zym |
| 120074 | tryptophan deaminase | - | ||
| 68368 | tryptophan deaminase | - | 4.1.99.1 | from API 20E |
| 120074 | tween esterase | + | ||
| 120074 | urease | + | 3.5.1.5 | |
| 68379 | urease | - | 3.5.1.5 | from API Coryne |
| 68368 | urease | - | 3.5.1.5 | from API 20E |
Global distribution of 16S sequence X85955 (>99% sequence identity) for Tsukamurella from Microbeatlas ![]()
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 20218 | Tsukamurella inchonensis 16S rRNA gene, strain IMMIB D-771T | X85955 | 1490 | 42777 | ||
| 11467 | Tsukamurella inchonensis strain ATCC700082 16S ribosomal RNA gene, complete sequence | AF283281 | 1512 | 42777 | ||
| 124043 | Tsukamurella inchonensis strain ATCC 700082 16S ribosomal RNA gene, partial sequence. | KX924564 | 1221 | 42777 |
| @ref | GC-content (mol%) | Method | |
|---|---|---|---|
| 67770 | 72 | high performance liquid chromatography (HPLC) |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Enzymology | Catheter-related bloodstream infection by Tsukamurella inchonensis in an immunocompromised patient. | Takebe I, Sawabe E, Ohkusu K, Tojo N, Tohda S. | J Clin Microbiol | 10.1128/jcm.00421-14 | 2014 | |
| Enzymology | Detection of Tropheryma whipplei DNA in clinical specimens by LightCycler real-time PCR. | Sloan LM, Rosenblatt JE, Cockerill FR. | J Clin Microbiol | 10.1128/jcm.43.7.3516-3518.2005 | 2005 | |
| Metabolism | Structural elucidation of Tsukamurella pulmonis neutral polysaccharide and its visualization in infected mouse tissues by specific monoclonal antibodies. | Saeed A, Pasciak M, Gorska S, Ceremuga I, Gamian E, Ziolkowski P, Drab M, Gamian A. | Sci Rep | 10.1038/s41598-018-29864-y | 2018 | |
| Enzymology | Identification of a cation-specific channel (TipA) in the cell wall of the gram-positive mycolata Tsukamurella inchonensis: the gene of the channel-forming protein is identical to mspA of Mycobacterium smegmatis and mppA of Mycobacterium phlei. | Dorner U, Maier E, Benz R | Biochim Biophys Acta | 10.1016/j.bbamem.2004.09.001 | 2004 | |
| Phylogeny | Tsukamurella strandjordae sp. nov., a proposed new species causing sepsis. | Kattar MM, Cookson BT, Carlson LD, Stiglich SK, Schwartz MA, Nguyen TT, Daza R, Wallis CK, Yarfitz SL, Coyle MB. | J Clin Microbiol | 10.1128/jcm.39.4.1467-1476.2001 | 2001 | |
| Phylogeny | Tsukamurella serpentis sp. nov., isolated from the oral cavity of Chinese cobras (Naja atra). | Tang Y, Teng JLL, Cheung CLW, Ngan AHY, Huang Y, Wong SSY, Yip EKT, Ng KHL, Que TL, Lau SKP, Woo PCY | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001187 | 2016 | |
| Phylogeny | Tsukamurella tyrosinosolvens sp. nov. | Yassin AF, Rainey FA, Burghardt J, Brzezinka H, Schmitt S, Seifert P, Zimmermann O, Mauch H, Gierth D, Lux I, Schaal KP | Int J Syst Bacteriol | 10.1099/00207713-47-3-607 | 1997 | |
| Phylogeny | Tsukamurella pulmonis sp. nov. | Yassin AF, Rainey FA, Brzezinka H, Burghardt J, Rifai M, Seifert P, Feldmann K, Schaal KP | Int J Syst Bacteriol | 10.1099/00207713-46-2-429 | 1996 | |
| Phylogeny | Tsukamurella inchonensis sp. nov. | Yassin AF, Rainey FA, Brzezinka H, Burghardt J, Lee HJ, Schaal KP | Int J Syst Bacteriol | 10.1099/00207713-45-3-522 | 1995 |
| #11467 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44067 |
| #19624 | Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig . |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 ) |
| #39097 | ; Curators of the CIP; |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68368 | Automatically annotated from API 20E . |
| #68379 | Automatically annotated from API Coryne . |
| #68382 | Automatically annotated from API zym . |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #120074 | Collection of Institut Pasteur ; Curators of the CIP; CIP 104790 |
| #124043 | Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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