Actinomadura viridilutea (DSM 44433, IFO 14480, IMET 9742)

Name: Actinomadura viridilutea (DSM 44433, IFO 14480, IMET 9742)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16968

Type strain

Culture col. no. DSM 44433 IFO 14480 IMET 9742 INMI 187 JCM 3398 6 more

NCBI tax ID(s) 58112

Special Collections DSMZ JCM KCTC Wink compendium

History <- IFO <- VKM <- INMI A. Shimazu <-- IFO 14480 <-- VKM <-- INMI 187.

Links

Actinomadura viridilutea DSM 44433 is a spore-forming, thermophilic prokaryote that builds an aerial mycelium and was isolated from light-gray desert soil.

spore-forming thermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 92965

ATCC 33925,JCM 3398,DSM 44433,IMET 9742,DSM 43934,IFO 14480,JCM 7346,CCRC 13638,KCTC 9185,NBRC 100039,NBRC 14480,BCRC 13638

@ref 20215

Last LPSN update 2025-12-16 09:50:58

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Streptosporangiales

Family Thermomonosporaceae

Genus Actinomadura

Species Actinomadura viridilutea

Full scientific name Actinomadura viridilutea (Agre and Guzeva 1975) Zhang et al. 2001

Synonyms (4)

"Excellospora rubrobrunea"
Actinomadura rubrobrunea
"Micropolyspora rubrobrunea"
Excellospora viridilutea

BacDive ID	Other strains from **Actinomadura viridilutea** (4)	Type strain
16970	A. viridilutea 2991, DSM 43750, ATCC 49883, IFO 15178, IMET ...
16971	A. viridilutea DSM 43751, VKM Ac-775
16972	A. viridilutea N2991, DSM 46178, IMET 9590
128622	A. viridilutea STH00380(ZIMET), ST 27511, HKI 0380

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	positive	99.8

Colony morphology

@ref	Colony color	Incubation period	Medium used
20059	Beige (1001)	10-14 days	ISP 2
20059	Beige (1001)	10-14 days	ISP 3
20059	Beige (1001)	10-14 days	ISP 4
20059	Beige (1001)	10-14 days	ISP 5
20059	Beige (1001)	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
20059			ISP 2
20059			ISP 3
20059	Aerial Mycelium	Turquoise blue (5018)	ISP 4
20059			ISP 5
20059			ISP 6
20059			ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11744	ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)	Medium recipe at MediaDive	Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
20059	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20059	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20059	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20059	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20059	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
11744	R2A MEDIUM (DSMZ Medium 830)	Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
11744	positive	growth	45	thermophilic
20059	positive	optimum	45	thermophilic
67770	positive	growth	50	thermophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125438	aerobe	91.115
125439	obligate aerobe	99.3

Spore formation

@ref	Spore description	Spore formation	Confidence
20059	Formation of spore chains, spore surface smooth
125439			91.2

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	kanosamine biosynthesis II	100	2 of 2
66794	methylglyoxal degradation	100	5 of 5
66794	butanoate fermentation	100	4 of 4
66794	valine metabolism	100	9 of 9
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	lipoate biosynthesis	100	5 of 5
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	starch degradation	100	10 of 10
66794	biotin biosynthesis	100	4 of 4
66794	propanol degradation	100	7 of 7
66794	denitrification	100	2 of 2
66794	hydrogen production	100	5 of 5
66794	molybdenum cofactor biosynthesis	100	9 of 9
66794	teichoic acid biosynthesis	100	1 of 1
66794	ppGpp biosynthesis	100	4 of 4
66794	sulfopterin metabolism	100	4 of 4
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	Entner Doudoroff pathway	100	10 of 10
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	cardiolipin biosynthesis	100	7 of 7
66794	threonine metabolism	100	10 of 10
66794	ethanol fermentation	100	2 of 2
66794	acetate fermentation	100	4 of 4
66794	vitamin K metabolism	100	5 of 5
66794	formaldehyde oxidation	100	3 of 3
66794	photosynthesis	100	14 of 14
66794	palmitate biosynthesis	100	22 of 22
66794	coenzyme A metabolism	100	4 of 4
66794	adipate degradation	100	2 of 2
66794	daunorubicin biosynthesis	100	9 of 9
66794	suberin monomers biosynthesis	100	2 of 2
66794	cellulose degradation	100	5 of 5
66794	taurine degradation	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	flavin biosynthesis	93.33	14 of 15
66794	phenylalanine metabolism	92.31	12 of 13
66794	myo-inositol biosynthesis	90	9 of 10
66794	4-hydroxymandelate degradation	88.89	8 of 9
66794	serine metabolism	88.89	8 of 9
66794	CO2 fixation in Crenarchaeota	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	NAD metabolism	88.89	16 of 18
66794	ketogluconate metabolism	87.5	7 of 8
66794	gluconeogenesis	87.5	7 of 8
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	isoleucine metabolism	87.5	7 of 8
66794	tryptophan metabolism	86.84	33 of 38
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	aclacinomycin biosynthesis	85.71	6 of 7
66794	glutathione metabolism	85.71	12 of 14
66794	citric acid cycle	85.71	12 of 14
66794	purine metabolism	85.11	80 of 94
66794	phenol degradation	85	17 of 20
66794	urea cycle	84.62	11 of 13
66794	leucine metabolism	84.62	11 of 13
66794	glycolate and glyoxylate degradation	83.33	5 of 6
66794	selenocysteine biosynthesis	83.33	5 of 6
66794	glutamate and glutamine metabolism	82.14	23 of 28
66794	proline metabolism	81.82	9 of 11
66794	pentose phosphate pathway	81.82	9 of 11
66794	methionine metabolism	80.77	21 of 26
66794	propionate fermentation	80	8 of 10
66794	glycine betaine biosynthesis	80	4 of 5
66794	pyrimidine metabolism	80	36 of 45
66794	factor 420 biosynthesis	80	4 of 5
66794	glycogen metabolism	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	heme metabolism	78.57	11 of 14
66794	tetrahydrofolate metabolism	78.57	11 of 14
66794	d-mannose degradation	77.78	7 of 9
66794	allantoin degradation	77.78	7 of 9
66794	vitamin B1 metabolism	76.92	10 of 13
66794	vitamin B12 metabolism	76.47	26 of 34
66794	glycolysis	76.47	13 of 17
66794	non-pathway related	76.32	29 of 38
66794	alanine metabolism	75.86	22 of 29
66794	degradation of sugar alcohols	75	12 of 16
66794	glycogen biosynthesis	75	3 of 4
66794	cyclohexanol degradation	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	dTDPLrhamnose biosynthesis	75	6 of 8
66794	metabolism of disaccharids	72.73	8 of 11
66794	oxidative phosphorylation	70.33	64 of 91
66794	isoprenoid biosynthesis	69.23	18 of 26
66794	lipid metabolism	67.74	21 of 31
66794	acetoin degradation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	arginine metabolism	66.67	16 of 24
66794	cyanate degradation	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	aspartate and asparagine metabolism	66.67	6 of 9
66794	histidine metabolism	65.52	19 of 29
66794	tyrosine metabolism	64.29	9 of 14
66794	d-xylose degradation	63.64	7 of 11
66794	phosphatidylethanolamine bioynthesis	61.54	8 of 13
66794	cysteine metabolism	61.11	11 of 18
66794	degradation of hexoses	61.11	11 of 18
66794	gallate degradation	60	3 of 5
66794	glycine metabolism	60	6 of 10
66794	lysine metabolism	59.52	25 of 42
66794	carotenoid biosynthesis	59.09	13 of 22
66794	degradation of aromatic, nitrogen containing compounds	58.33	7 of 12
66794	degradation of pentoses	57.14	16 of 28
66794	ubiquinone biosynthesis	57.14	4 of 7
66794	androgen and estrogen metabolism	56.25	9 of 16
66794	cholesterol biosynthesis	54.55	6 of 11
66794	sulfate reduction	53.85	7 of 13
66794	3-phenylpropionate degradation	53.33	8 of 15
66794	coenzyme M biosynthesis	50	5 of 10
66794	toluene degradation	50	2 of 4
66794	quinate degradation	50	1 of 2
66794	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	ascorbate metabolism	50	11 of 22
66794	phenylmercury acetate degradation	50	1 of 2
66794	CMP-KDO biosynthesis	50	2 of 4
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	bile acid biosynthesis, neutral pathway	47.06	8 of 17
66794	nitrate assimilation	44.44	4 of 9
66794	chlorophyll metabolism	44.44	8 of 18
66794	lipid A biosynthesis	44.44	4 of 9
66794	polyamine pathway	43.48	10 of 23
66794	creatinine degradation	40	2 of 5
66794	4-hydroxyphenylacetate degradation	40	4 of 10
66794	arachidonate biosynthesis	40	2 of 5
66794	degradation of sugar acids	40	10 of 25
66794	ethylmalonyl-CoA pathway	40	2 of 5
66794	D-cycloserine biosynthesis	40	2 of 5
66794	3-chlorocatechol degradation	40	2 of 5
66794	bacilysin biosynthesis	40	2 of 5
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	carnitine metabolism	37.5	3 of 8
66794	vitamin B6 metabolism	36.36	4 of 11
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	pantothenate biosynthesis	33.33	2 of 6
66794	methanogenesis from CO2	33.33	4 of 12
66794	sphingosine metabolism	33.33	2 of 6
66794	arachidonic acid metabolism	33.33	6 of 18
66794	IAA biosynthesis	33.33	1 of 3
66794	enterobactin biosynthesis	33.33	1 of 3
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	mevalonate metabolism	28.57	2 of 7
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	lactate fermentation	25	1 of 4
66794	catecholamine biosynthesis	25	1 of 4
66794	vitamin E metabolism	25	1 of 4
66794	alginate biosynthesis	25	1 of 4

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Desert
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Country	Country ISO 3 Code
11744	light-gray desert soil
67770	Light-gray desert soil	Russia	RUS

Taxonmaps

69479

Global distribution of 16S sequence D86943 (>99% sequence identity) for Actinomadura from Microbeatlas

Aquatic Samples	96
Soil Samples	285
Animal Samples	116
Plant Samples	45
Total Samples	542

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11744	1	Risk group (German classification) According to TRBA 466
20059	1	German classification According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM300179v1 assembly for Actinomadura viridilutea DSM 44433	scaffold	GCA_003001795	58112.4	2728369515	58112	37.8

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Actinomadura viridilutea gene for 16S ribosomal RNA, partial sequence	D86943	1475		58112
20218	Excellospora viridilutea 16S-23S ribosomal RNA intergenic spacer region, partial sequence	AF134110	418		58112

GC content

@ref	GC-content (mol%)	Method
67770	72.8	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Actinomadura viridilutea DSM 44433
NCBI: GCA_003001795

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	91.20	no
125439	motility	BacteriaNetⓘ	no	88.50	no
125439	gram_stain	BacteriaNetⓘ	positive	99.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinomadura viridilutea strain DSM 44433
PATRIC: 58112.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	84.60	no
125438	anaerobic	anaerobicⓘ	no	94.79	no
125438	spore-forming	spore-formingⓘ	yes	71.85	yes
125438	aerobic	aerobicⓘ	yes	91.12	no
125438	thermophilic	thermophileⓘ	no	93.91	no
125438	flagellated	motile2+ⓘ	no	89.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018
	Novel thrombolytic protease from the rare halophile Brachybacterium paraconglomeratum: bioprocessing and in vitro application.	Teja M, Jarso TS, Kumar DM, Godi S.	BMC Biotechnol	10.1186/s12896-025-00967-5	2025
	Heterologous expression and purification of keratinase from Actinomadura viridilutea DZ50: feather biodegradation and animal hide dehairing bioprocesses.	Ben Elhoul M, Zarai Jaouadi N, Bouacem K, Allala F, Rekik H, Mechri S, Khemir Ezzine H, Miled N, Jaouadi B.	Environ Sci Pollut Res Int	10.1007/s11356-020-11371-1	2021
Enzymology	Biochemical and molecular characterization of new keratinoytic protease from Actinomadura viridilutea DZ50.	Ben Elhoul M, Zarai Jaouadi N, Rekik H, Omrane Benmrad M, Mechri S, Moujehed E, Kourdali S, El Hattab M, Badis A, Bejar S, Jaouadi B.	Int J Biol Macromol	10.1016/j.ijbiomac.2016.07.009	2016
Genetics	A Newly Isolated Strain Lysobacter brunescens YQ20 and Its Performance on Wool Waste Biodegradation.	Ma Q, Zhang YN, Zheng X, Luan F, Han P, Zhang X, Yin Y, Wang X, Gao X.	Front Microbiol	10.3389/fmicb.2022.794738	2022
Phylogeny	Clarification of the relationship between the members of the family Thermomonosporaceae on the basis of 16S rDNA, 16S-23S rRNA internal transcribed spacer and 23S rDNA sequences and chemotaxonomic analyses.	Zhang Z, Kudo T, Nakajima Y, Wang Y.	Int J Syst Evol Microbiol	10.1099/00207713-51-2-373	2001

References

#11744	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44433
#20059	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinomadura viridilutea (DSM 44433, IFO 14480, IMET 9742)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_003001795

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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