Actinomadura viridilutea (DSM 43750, ATCC 49883, IFO 15178)

Name: Actinomadura viridilutea (DSM 43750, ATCC 49883, IFO 15178)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16970

Type strain

Strain Designation 2991

Culture col. no. DSM 43750 ATCC 49883 IFO 15178 IMET 9705 JCM 7345 10 more

NCBI tax ID(s) 115335

Special Collections DSMZ JCM KCTC CIP Wink compendium

History <- N.S. Agre, 2991 (Excellospora rubrobrunea) IMET 9705 <-- N. S. Agre 2991. CIP <- 1998, JCM <- IMET <- N.S. Agre: strain 2991, "Excellospora rubrobrunea", "Micropolyspora rubrobrunea"

Links

Actinomadura viridilutea 2991 is an obligate aerobe, Gram-positive, rod-shaped bacterium that builds an aerial mycelium.

Gram-positive rod-shaped obligate aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 43364

ATCC 49883,DSM 43750,JCM 7345,CIP 105486,IFO 15275,IMET 9705,VKM Ac-1470,IFO 15178,KCTC 9493,NBRC 15275,NBRC 15178,KACC 20071,BCRC 16817,CCRC 16817

@ref 20215

Last LPSN update 2026-02-09 11:22:06

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Streptosporangiales

Family Thermomonosporaceae

Genus Actinomadura

Species Actinomadura viridilutea

Full scientific name Actinomadura viridilutea (Agre and Guzeva 1975) Zhang et al. 2001

Synonyms (4)

"Excellospora rubrobrunea"
Actinomadura rubrobrunea
"Micropolyspora rubrobrunea"
Excellospora viridilutea

BacDive ID	Other strains from **Actinomadura viridilutea** (4)	Type strain
16968	A. viridilutea DSM 44433, IFO 14480, IMET 9742, INMI 187, ... (type strain)
16971	A. viridilutea DSM 43751, VKM Ac-775
16972	A. viridilutea N2991, DSM 46178, IMET 9590
128622	A. viridilutea STH00380(ZIMET), ST 27511, HKI 0380

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
125439	positive			99.9
122292	positive	rod-shaped

Colony morphology

@ref	Colony color	Incubation period	Medium used
19950	Beige	10-14 days	ISP 3

Multicellular morphology

@ref	Complex name	Complex color	Medium name
19950			ISP 2
19950	Aerial Mycelium	Cream (9001)	ISP 3
19950			ISP 4
19950			ISP 5
19950			ISP 6
19950			ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
19950	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
39303	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
11247	ISP MEDIUM 4 (DSMZ Medium 547)	Medium recipe at MediaDive	Name: ISP MEDIUM 4 (DSMZ Medium 547) Composition: Agar 19.98 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999002 g/l MgSO4 x 7 H2O 0.999002 g/l NaCl 0.999002 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
11247	OAT FLAKES AGAR WITH YEAST EXTRACT (DSMZ Medium 248)	Medium recipe at MediaDive	Name: OAT FLAKES AGAR WITH YEAST EXTRACT (DSMZ Medium 248) Composition: Agar 20.0 g/l Oat flakes 20.0 g/l Yeast extract 2.0 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l ZnSO4 x 7 H2O 0.001 g/l Distilled water
122292	CIP Medium 3	Medium recipe at CIP
122292	CIP Medium 271	Medium recipe at CIP
122292	CIP Medium 272	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
11247	positive	growth	50
19950	positive	optimum	55
39303	positive	growth	55
67770	positive	growth	50
122292	positive	growth	37-55
122292	negative	growth	5
122292	negative	growth	15
122292	negative	growth	22
122292	negative	growth	30

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
122292	obligate aerobe
125438	aerobe	91.115
125439	obligate aerobe	99.7

Spore formation

@ref	Spore formation	Confidence
19950
125439		91.4

Halophily

@ref	Salt	Growth	Tested relation	Concentration
122292	NaCl	positive	growth	0-4 %
122292	NaCl		growth	6 %
122292	NaCl		growth	8 %
122292	NaCl		growth	10 %

Observation

67770

Observationquinones: MK-9(H₆), MK-9(H₈), MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
122292	4853 ChEBI	esculin	+	hydrolysis
122292	17632 ChEBI	nitrate	+	reduction
122292	17632 ChEBI	nitrate	-	respiration
122292	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
122292	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
122292	15688 ChEBI	acetoin	-
122292	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
122292	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
122292	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	+		from API zym
122292	gamma-glutamyltransferase	-	2.3.2.2
122292	gelatinase	+
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
122292	oxidase	-
122292	phenylalanine ammonia-lyase	-	4.3.1.24
68382	trypsin	-	3.4.21.4	from API zym
122292	tryptophan deaminase	-
122292	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
122292	-	+	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
39303			United Kingdom	GBR	Europe
67770	Soil
122292	Environment, Soil	England	United Kingdom	GBR	Europe

Taxonmaps

69479

Global distribution of 16S sequence EU637008 (>99% sequence identity) for Actinomadura from Microbeatlas

Aquatic Samples	96
Soil Samples	285
Animal Samples	116
Plant Samples	45
Total Samples	542

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11247	1	Risk group (German classification) According to TRBA 466
19950	1	German classification According to TRBA 466
122292	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM155223v1 assembly for Actinomadura rubrobrunea NBRC 15275	contig	GCA_001552235	1220567.3	2731957576	1220567	29.99

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Actinomadura rubrobrunea strain DSM 43750 16S ribosomal RNA gene, partial sequence	EU637008	1490		115335

GC content

@ref	GC-content (mol%)	Method
67770	72.8	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Actinomadura rubrobrunea NBRC 15275
NCBI: GCA_001552235

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	91.40	no
125439	motility	BacteriaNetⓘ	no	88.10	no
125439	gram_stain	BacteriaNetⓘ	positive	99.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinomadura rubrobrunea NBRC 15275
PATRIC: 1220567.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	84.60	no
125438	anaerobic	anaerobicⓘ	no	94.79	yes
125438	spore-forming	spore-formingⓘ	yes	71.85	yes
125438	aerobic	aerobicⓘ	yes	91.12	no
125438	thermophilic	thermophileⓘ	no	93.91	no
125438	flagellated	motile2+ⓘ	no	89.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018

References

#11247	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43750
#19950	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#39303	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#122292	Collection of Institut Pasteur ; Curators of the CIP; CIP 105486
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinomadura viridilutea (DSM 43750, ATCC 49883, IFO 15178)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

Isolation, sampling and environmental information

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001552235

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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