Actinomadura viridilutea 2991 | DSM 43750, ATCC 49883, IFO 15178, IMET 9705, JCM 7345, NBRC 15178, BCRC 16817, CIP 105486, IFO 15275, IMSNU 22192, INMI 2991, KCTC 9493, NBRC 15275, VKM Ac-1470

Culture medium

ISP MEDIUM 4 (DSMZ Medium 547)

Culture medium growth

Culture medium link

Culture medium

OAT FLAKES AGAR WITH YEAST EXTRACT (DSMZ Medium 248)

Culture medium growth

Culture medium link

Culture medium

CIP Medium 3

Culture medium growth

Culture medium link

Culture medium

CIP Medium 271

Culture medium growth

Culture medium link

Culture medium

CIP Medium 272

Culture medium growth

Culture medium link

	Kind of temperature		Temperature
[Ref.: #11247]		growth	50 °C
[Ref.: #19950]		optimum	55 °C
[Ref.: #39303]		growth	55 °C
[Ref.: #67770]		growth	50 °C
[Ref.: #122292]		growth	37-55 °C
[Ref.: #122292]	no	growth	5 °C
[Ref.: #122292]	no	growth	15 °C
[Ref.: #122292]	no	growth	22 °C
[Ref.: #122292]	no	growth	30 °C

[Ref.: #11247]	Temperature range	thermophilic
[Ref.: #19950]	Temperature range	thermophilic
[Ref.: #39303]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic
[Ref.: #122292]	Temperature range	psychrophilic
[Ref.: #122292]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

Oxygen tolerance

obligate aerobe

[Ref.: #19950]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Salt	Tested relation		Salt conc.
[Ref.: #122292]	NaCl		growth	0-4	%
[Ref.: #122292]	NaCl	no	growth	6	%
[Ref.: #122292]	NaCl	no	growth	8	%
[Ref.: #122292]	NaCl	no	growth	10	%

[Ref.: #67770]

Observation

quinones: MK-9(H₆), MK-9(H₈), MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #122292]	esculin ChEBI	+	hydrolysis
[Ref.: #122292]	nitrate ChEBI	+	reduction
[Ref.: #122292]	nitrate ChEBI	-	respiration
[Ref.: #122292]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #122292]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #122292]		acetoin ChEBI	-
[Ref.: #122292]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #122292]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #122292]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #122292]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #122292]	gelatinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #122292]	oxidase	-
[Ref.: #122292]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #122292]	tryptophan deaminase	-
[Ref.: #122292]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122292]	8739	-	+	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Soil

[Ref.: #122292]	Sample type/isolated from	Environment, Soil
[Ref.: #122292]	Geographic location (country and/or sea, region)	England
[Ref.: #122292]	Country	United Kingdom
[Ref.: #122292]	Country ISO 3 Code	GBR
[Ref.: #122292]	Continent	Europe

[Ref.: #39303]	Country	United Kingdom
[Ref.: #39303]	Country ISO 3 Code	GBR
[Ref.: #39303]	Continent	Europe
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence EU637008 (>99% sequence identity) for Actinomadura from Microbeatlas

Aquatic Samples	96
Soil Samples	285
Animal Samples	116
Plant Samples	45
Total Samples	542

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11247]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #19950]	Biosafety level	1	German classification
[Ref.: #122292]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Actinomadura rubrobrunea strain DSM 43750 16S ribosomal RNA gene, partial sequence	EU637008	1490	ENA	115335 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Actinomadura rubrobrunea NBRC 15275	GCA_001552235	contig	GenBank	1220567 tax ID
[Ref.: #66792]	Actinomadura rubrobrunea NBRC 15275	1220567.3	wgs	PATRIC	1220567 tax ID	*
[Ref.: #66792]	Actinomadura rubrobrunea NBRC 15275	2731957576	draft	JGI IMG/M	1220567 tax ID	*

[Ref.: #67770]

GC-content

72.8 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Actinomadura rubrobrunea NBRC 15275 PATRIC: 1220567.3
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinomadura rubrobrunea NBRC 15275 PATRIC: 1220567.3
[Ref.: #69480]	motile	no	92.333	no
[Ref.: #69480]	gram-positive	yes	87.075	no
[Ref.: #69480]	anaerobic	no	98.422	no
[Ref.: #69480]	aerobic	yes	86.892	no
[Ref.: #69480]	halophile	no	87.233	no
[Ref.: #69480]	spore-forming	yes	91.75	no
[Ref.: #69480]	glucose-util	yes	91.053	no
[Ref.: #69480]	flagellated	no	98.185	no
[Ref.: #69480]	thermophile	yes	64.62	yes
[Ref.: #69480]	glucose-ferment	no	90.402	no

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