Name: Pyrococcus horikoshii OT-3
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 16857

Type strain:

Species: Pyrococcus horikoshii

Strain Designation: OT-3

Culture col. no.: DSM 12428, JCM 9974, NBRC 100139

Strain history: C. Kato <-- F. Robb OT-3.

NCBI tax ID(s): 53953 (species)

SI-ID 44181

ATCC 700860, JCM 9974, DSM 12428, NBRC 100139

Other strains from Pyrococcus horikoshii

P. horikoshii JA-1, DSM 13347, JCM 9975

Section

Pyrococcus horikoshii OT-3 is an anaerobe, hyperthermophilic archaeon that was isolated from marine hydrothermal vent at 1395 m depth.

anaerobe
hyperthermophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Thermococci*
	Order	*Thermococcales*
	Family	*Thermococcaceae*
	Genus	*Pyrococcus*
	Species	**Pyrococcus horikoshii**
	Full Scientific Name (LPSN)	Pyrococcus horikoshii González et al. 1999

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4716]

Culture medium

PYROCOCCUS MEDIUM (DSMZ Medium 377)

[Ref.: #4716]

Culture medium growth

[Ref.: #4716]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4716]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #4716]			growth	95 °C
[Ref.: #67770]			growth	95 °C

[Ref.: #4716]	Temperature range	hyperthermophilic
[Ref.: #67770]	Temperature range	hyperthermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #4716]

Oxygen tolerance

anaerobe

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4716]	Sample type/isolated from	marine hydrothermal vent at 1395 m depth
[Ref.: #4716]	Geographic location (country and/or sea, region)	Pacific Ocean, Okinawa Trough
[Ref.: #4716]	Country	Japan
[Ref.: #4716]	Country ISO 3 Code	JPN
[Ref.: #4716]	Continent	Asia
[Ref.: #4716]	Geographic location	27.5500°/126.9330°

[Ref.: #67770]	Sample type/isolated from	Hydrothermal vent at the Okinawa Trough
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Hydrothermal vent
#Environmental	#Aquatic	#Marine
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4716]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Pyrococcus horikoshii strain DSM 12428 16S ribosomal RNA gene, partial sequence	AY099169	1454	ENA	53953 tax ID	*
[Ref.: #20218]	Pyrococcus horikoshii strain DSM 12428 16S-23S ribosomal RNA intergenic spacer region, complete sequence	AY099205	190	ENA	53953 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Pyrococcus horikoshii OT3	GCA_000011105	complete	GenBank	70601 tax ID

[Ref.: #4716]

GC-content

41 mol%

sequence analysis

Availability in culture collections External links

[Ref.: #4716]

Culture collection no.

DSM 12428, JCM 9974, NBRC 100139

[Ref.: #85866]

SI-ID 44181

Literature:

Only first 10 entries are displayed. Click here to see all.

	Title	Authors	Journal	DOI	Year
Metabolism	Crystal structure of a novel type of ornithine delta-aminotransferase from the hyperthermophilic archaeon Pyrococcus horikoshii.	Kawakami R, Ohshida T, Hayashi J, Yoneda K, Furumoto T, Ohshima T, Sakuraba H	Int J Biol Macromol	10.1016/j.ijbiomac.2022.03.114	2022	*
Metabolism	First characterization of an archaeal amino acid racemase with broad substrate specificity from the hyperthermophile Pyrococcus horikoshii OT-3.	Kawakami R, Sakuraba H, Ohmori T, Ohshima T	J Biosci Bioeng	10.1016/j.jbiosc.2017.02.004	2017	*
Metabolism	Identification of a novel amino acid racemase from a hyperthermophilic archaeon Pyrococcus horikoshii OT-3 induced by D-amino acids.	Kawakami R, Ohmori T, Sakuraba H, Ohshima T	Amino Acids	10.1007/s00726-015-2001-6	2015	*
Enzymology	Comparative analysis of the catalytic components in the archaeal dye-linked L-proline dehydrogenase complexes.	Kawakami R, Noguchi C, Higashi M, Sakuraba H, Ohshima T	Appl Microbiol Biotechnol	10.1007/s00253-012-4201-2	2012	*
Enzymology	A second novel dye-linked L-proline dehydrogenase complex is present in the hyperthermophilic archaeon Pyrococcus horikoshii OT-3.	Kawakami R, Sakuraba H, Tsuge H, Goda S, Katunuma N, Ohshima T	FEBS J	10.1111/j.1742-4658.2005.04810.x	2005	*
Enzymology	Oxidative stress response in an anaerobic hyperthermophilic archaeon: presence of a functional peroxiredoxin in Pyrococcus horikoshii.	Kawakami R, Sakuraba H, Kamohara S, Goda S, Kawarabayasi Y, Ohshima T	J Biochem	10.1093/jb/mvh157	2004	*
Enzymology	L-aspartate oxidase is present in the anaerobic hyperthermophilic archaeon Pyrococcus horikoshii OT-3: characteristics and role in the de novo biosynthesis of nicotinamide adenine dinucleotide proposed by genome sequencing.	Sakuraba H, Satomura T, Kawakami R, Yamamoto S, Kawarabayasi Y, Kikuchi H, Ohshima T	Extremophiles	10.1007/s00792-001-0254-3	2002	*
	A Novel PLP-Dependent Alanine/Serine Racemase From the Hyperthermophilic Archaeon Pyrococcus horikoshii OT-3.	Kawakami R, Ohshida T, Sakuraba H, Ohshima T	Front Microbiol	10.3389/fmicb.2018.01481	2018	*

References

#4716

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12428

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#85866

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID44181.1 )

* These data were automatically processed and therefore are not curated

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