Name: Pyrococcus woesei DSM 3773
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 16856

Type strain:

Species: Pyrococcus woesei

Culture col. no.: DSM 3773, JCM 8421, ATCC 49860, CGMCC 1.2515

Strain history: DSM 3773 <-- W. Zillig Vul4.

NCBI tax ID(s): 2262 (species)

SI-ID 47759

DSM 3773, JCM 8421, ATCC 49860, CGMCC 1.2515

Special Collections

DSMZ
JCM

Section

Pyrococcus woesei DSM 3773 is an anaerobe archaeon that was isolated from marine solfatara.

anaerobe
16S sequence
Archaea

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Thermococci*
	Order	*Thermococcales*
	Family	*Thermococcaceae*
	Genus	*Pyrococcus*
	Species	**Pyrococcus woesei**
	Full Scientific Name (LPSN)	Pyrococcus woesei Zillig 1988

Synonym

"Pyrococcus furiosus subsp. woesei"

Information on culture and growth conditions Culture and growth conditions

[Ref.: #1469]

Culture medium

PYROCOCCUS MEDIUM (DSMZ Medium 377)

[Ref.: #1469]

Culture medium growth

[Ref.: #1469]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1469]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #1469]			growth	97 °C
[Ref.: #67770]			growth	95 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #1469]

Oxygen tolerance

anaerobe

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #1469]	Sample type/isolated from	marine solfatara
[Ref.: #1469]	Geographic location (country and/or sea, region)	Vulcano island
[Ref.: #1469]	Country	Italy
[Ref.: #1469]	Country ISO 3 Code	ITA
[Ref.: #1469]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Hot sediments in vents of marine solfatatras at the beach of Vulcano Island
[Ref.: #67770]	Country	Italy
[Ref.: #67770]	Country ISO 3 Code	ITA
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Terrestrial	#Volcanic

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY519654 (>99% sequence identity) for Pyrococcus furiosus from Microbeatlas

Aquatic Samples	4
Soil Samples
Animal Samples
Plant Samples
Total Samples	4

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1469]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #1469]	Pyrococcus woesei partial 16S rRNA gene, type strain DSM 3773T	HE654000	1465	GenBank ENA	2262 tax ID
[Ref.: #67770]	Pyrococcus woesei 16S ribosomal RNA gene, 16S-23S ribosomal RNA intergenic spacer, and tRNA-Ala gene, complete sequence; and 23S ribosomal RNA gene, partial sequence	AY519654	1807	GenBank ENA	2262 tax ID

[Ref.: #1469]	GC-content	37.5 mol%
[Ref.: #67770]	GC-content	37.5 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #1469]

Culture collection no.

DSM 3773, JCM 8421, ATCC 49860, CGMCC 1.2515

[Ref.: #85865]

SI-ID 47759

Literature:

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Microwave-Assisted Synthesis of Glycoconjugates by Transgalactosylation with Recombinant Thermostable beta-Glycosidase from Pyrococcus.	Henze M, Merker D, Elling L	Int J Mol Sci	10.3390/ijms17020210	2016	*
Enzymology	Cloning of the thermostable alpha-amylase gene from Pyrococcus woesei in Escherichia coli: isolation and some properties of the enzyme.	Grzybowska B, Szweda P, Synowiecki J	Mol Biotechnol	10.1385/MB:26:2:101	2004	*
Enzymology	Cloning and nucleotide sequence of the thermostable beta-galactosidase gene from Pyrococcus woesei in Escherichia coli and some properties of the isolated enzyme.	Dabrowski S, Maciunska J, Synowiecki J	Mol Biotechnol	10.1007/BF02740841	1998	*

References

#1469

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 3773

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#85865

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID47759.1 )

* These data were automatically processed and therefore are not curated

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