Pyrococcus woesei (DSM 3773, JCM 8421, ATCC 49860)

Name: Pyrococcus woesei (DSM 3773, JCM 8421, ATCC 49860)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16856

Type strain

Culture col. no. DSM 3773 JCM 8421 ATCC 49860 CGMCC 1.2515

NCBI tax ID(s) 2262

Special Collections DSMZ JCM

History DSMZ <- W. Zillig, Vul4 JCM <-- DSM 3773 <-- W. Zillig Vul4.

Links

Pyrococcus woesei DSM 3773 is an anaerobe archaeon that was isolated from marine solfatara.

anaerobe genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 47759

DSM 3773,JCM 8421,ATCC 49860,CGMCC 1.2515

@ref 20215

Last LPSN update 2026-05-29 08:28:52

Domain Archaea

Phylum Methanobacteriota

Class Thermococci

Order Thermococcales

Family Thermococcaceae

Genus Pyrococcus

Species Pyrococcus woesei

Full scientific name Pyrococcus woesei Zillig 1988

Synonyms (1)

"Pyrococcus furiosus subsp. woesei"

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
1469	PYROCOCCUS MEDIUM (DSMZ Medium 377)		Medium recipe at MediaDive	Name: PYROCOCCUS MEDIUM (DSMZ Medium 377) Composition: Sulfur 30.0 g/l Peptone 5.0 g/l MgCl2 x 6 H2O 2.75 g/l Yeast extract 1.0 g/l KH2PO4 0.5 g/l Na2S x 9 H2O 0.5 g/l KCl 0.33 g/l NaBr 0.05 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l (NH4)2SO4 0.01 g/l SrCl2 x 6 H2O 0.007 g/l Citric acid 0.005 g/l MnSO4 x H2O 0.005 g/l CoSO4 x 7 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.0018 g/l CaCl2 x 2 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l CuSO4 x 5 H2O 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l H3BO3 0.0001 g/l KI 5e-05 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
1469	positive	growth	97
67770	positive	growth	95

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
1469	anaerobe
125439	anaerobe	98.835

Spore formation

@ref	Spore formation	Confidence
125439		91.082

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Environmental	#Terrestrial	#Volcanic

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
1469	marine solfatara	Vulcano island	Italy	ITA	Europe
67770	Hot sediments in vents of marine solfatatras at the beach of Vulcano Island		Italy	ITA	Europe

Taxonmaps

69479

Global distribution of 16S sequence AY519654 (>99% sequence identity) for Pyrococcus furiosus from Microbeatlas

Aquatic Samples	4
Soil Samples	0
Animal Samples	0
Plant Samples	0
Total Samples	4

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1469	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM5049610v1 assembly for Pyrococcus woesei DSM 3773	complete	GCA_050496105	2262	97.59

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
1469	Pyrococcus woesei partial 16S rRNA gene, type strain DSM 3773T	HE654000	1465		2262
67770	Pyrococcus woesei 16S ribosomal RNA gene, 16S-23S ribosomal RNA intergenic spacer, and tRNA-Ala gene, complete sequence; and 23S ribosomal RNA gene, partial sequence	AY519654	1807		2262

GC content

@ref	GC-content (mol%)	Method
1469	37.5
67770	37.5	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM5049610v1 assembly for Pyrococcus woesei DSM 3773
NCBI: GCA_050496105

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	98.84	no
125439	gram_stain	BacteriaNetⓘ	negative	40.27	no
125439	motility	BacteriaNetⓘ	yes	44.31	no
125439	spore_formation	BacteriaNetⓘ	no	91.08	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genome sequence of Pyrococcus woesei DSM 3773^T-an anaerobic, hyperthermophilic archaeum from heated marine solfataras.	Drew S, St John E, Bartlett M.	Microbiol Resour Announc	10.1128/mra.00528-25	2025
	Cloning, Expression, and Purification of Hyperthermophile alpha-Amylase from Pyrococcus woesei.	Ghasemi A, Ghafourian S, Vafaei S, Mohebi R, Farzi M, Taherikalani M, Sadeghifard N.	Osong Public Health Res Perspect	10.1016/j.phrp.2015.10.003	2015
Enzymology	Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei.	Ghasemi A, Salmanian AH, Sadeghifard N, Salarian AA, Gholi MK.	Iran J Microbiol		2011
	Isolation and some properties of the thermostable beta-balactosidase of Pyrococcus woesei expressed in Escherichia coli	Synowiecki J, Maciunska J.	J Food Biochem		2002
	Synthetic linear glycopolymers and their biological applications.	Qin Q, Lang S, Huang X.	J Carbohydr Chem	10.1080/07328303.2021.1928156	2021
Enzymology	Biotechnological applications of archaeal enzymes from extreme environments.	Cabrera MA, Blamey JM.	Biol Res	10.1186/s40659-018-0186-3	2018
Phylogeny	A proposal to rename the hyperthermophile Pyrococcus woesei as Pyrococcus furiosus subsp. woesei.	Kanoksilapatham W, Gonzalez JM, Maeder DL, DiRuggiero J, Robb FT.	Archaea	10.1155/2004/513563	2004
	Nucleotide sequence of the 5S ribosomal RNA of the archaebacterium Pyrococcus woesei.	De Wachter R, Willekens P, Zillig W.	Nucleic Acids Res	10.1093/nar/17.14.5848	1989
Enzymology	Isolation and characterization of a heat-stable pullulanase from the hyperthermophilic archaeon Pyrococcus woesei after cloning and expression of its gene in Escherichia coli.	Rudiger A, Jorgensen PL, Antranikian G.	Appl Environ Microbiol	10.1128/aem.61.2.567-575.1995	1995
Enzymology	Glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaebacterium Pyrococcus woesei: characterization of the enzyme, cloning and sequencing of the gene, and expression in Escherichia coli.	Zwickl P, Fabry S, Bogedain C, Haas A, Hensel R.	J Bacteriol	10.1128/jb.172.8.4329-4338.1990	1990
Phylogeny	Discontinuous occurrence of the hsp70 (dnaK) gene among Archaea and sequence features of HSP70 suggest a novel outlook on phylogenies inferred from this protein.	Gribaldo S, Lumia V, Creti R, Conway de Macario E, Sanangelantoni A, Cammarano P.	J Bacteriol	10.1128/jb.181.2.434-443.1999	1999
Phylogeny	PAV1, the first virus-like particle isolated from a hyperthermophilic euryarchaeote, "Pyrococcus abyssi".	Geslin C, Le Romancer M, Erauso G, Gaillard M, Perrot G, Prieur D.	J Bacteriol	10.1128/jb.185.13.3888-3894.2003	2003
	Keratin Degradation by Fervidobacterium pennavorans, a Novel Thermophilic Anaerobic Species of the Order Thermotogales.	Friedrich AB, Antranikian G.	Appl Environ Microbiol	10.1128/aem.62.8.2875-2882.1996	1996
	Compilation of 5S rRNA and 5S rRNA gene sequences.	Specht T, Wolters J, Erdmann VA.	Nucleic Acids Res	10.1093/nar/18.suppl.2215	1990
Enzymology	Microwave-Assisted Synthesis of Glycoconjugates by Transgalactosylation with Recombinant Thermostable beta-Glycosidase from Pyrococcus.	Henze M, Merker D, Elling L	Int J Mol Sci	10.3390/ijms17020210	2016
Enzymology	Cloning of the thermostable alpha-amylase gene from Pyrococcus woesei in Escherichia coli: isolation and some properties of the enzyme.	Grzybowska B, Szweda P, Synowiecki J	Mol Biotechnol	10.1385/MB:26:2:101	2004
Enzymology	Cloning and nucleotide sequence of the thermostable beta-galactosidase gene from Pyrococcus woesei in Escherichia coli and some properties of the isolated enzyme.	Dabrowski S, Maciunska J, Synowiecki J	Mol Biotechnol	10.1007/BF02740841	1998

References

#1469	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 3773
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Pyrococcus woesei (DSM 3773, JCM 8421, ATCC 49860)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_050496105

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media