Acidianus ambivalens (DSM 3772, JCM 9191, LEI 10)

Name: Acidianus ambivalens (DSM 3772, JCM 9191, LEI 10)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16643

Type strain

Culture col. no. DSM 3772 JCM 9191 LEI 10

NCBI tax ID(s) 2283

Special Collections DSMZ JCM

History <- W. Zillig, Max Planck Inst. Biochem., Martinsried, Germany; LEI 10 <- W. Zillig; {1984} DSM 3772 <-- W. Zillig Lei 10.

Links

Acidianus ambivalens DSM 3772 is an anaerobe archaeon that was isolated from solfataric mud.

anaerobe genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 47758

DSM 3772,JCM 9191,CIP 104912

@ref 20215

Last LPSN update 2026-02-09 11:22:01

Domain Archaea

Phylum Thermoproteota

Class Thermoprotei

Order Sulfolobales

Family Sulfolobaceae

Genus Acidianus

Species Acidianus ambivalens

Full scientific name Acidianus ambivalens (Zillig and Böck 1987) Fuchs et al. 1996

Synonyms (1)

Desulfurolobus ambivalens

Morphology

Colony morphology

1468

Incubation period2-3 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
1468	ACIDIANUS MEDIUM (ANAEROBIC) (DSMZ Medium 358a)		Medium recipe at MediaDive	Name: ACIDIANUS MEDIUM (ANAEROBIC) (DSMZ Medium 358a; with strain-specific modifications) Composition: Sulfur 4.9505 g/l (NH4)2SO4 1.28713 g/l KH2PO4 0.277228 g/l MgSO4 x 7 H2O 0.247525 g/l CaCl2 x 2 H2O 0.0693069 g/l Yeast extract 0.019802 g/l FeCl3 x 6 H2O 0.019802 g/l Na2B4O7 x 10 H2O 0.00445545 g/l MnCl2 x 4 H2O 0.00178218 g/l ZnSO4 x 7 H2O 0.000217822 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Na2MoO4 x 2 H2O 2.9703e-05 g/l VOSO4 x 2 H2O 2.9703e-05 g/l CoSO4 x 7 H2O 9.90099e-06 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
1468	positive	growth	80
67770	positive	growth	80

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
1468	anaerobe
125439	anaerobe	92.4

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Mud (Sludge)
#Environmental	#Terrestrial	#Volcanic

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
1468	solfataric mud	Leihnukur fissure	Iceland	ISL	Europe
67770	Solfataric waterhole at Leirhukur fissure		Iceland	ISL	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1468	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM972901v1 assembly for Acidianus ambivalens LEI 10	complete	GCA_009729015	2283.11	8065311606	2283	97.04
67770	ASM942888v1 assembly for Acidianus ambivalens DSM 3772	contig	GCA_009428885	2283.10	8065314359	2283	64.2

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	D.ambivalens DNA for 16S ribosomal RNA gene	X90484	1422		2283
1468	Acidianus ambivalens 16S rRNA gene, almost full-length sequence	D85506	1462		2283

GC content

1468

GC-content (mol%)32.7

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Acidianus ambivalens DSM 3772
NCBI: GCA_009428885

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	64.90	no
125439	motility	BacteriaNetⓘ	no	66.60	no
125439	gram_stain	BacteriaNetⓘ	negative	83.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	92.40	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Genome Sequences of Five Type Strain Members of the Archaeal Family Sulfolobaceae, Acidianus ambivalens, Acidianus infernus, Stygiolobus azoricus, Sulfuracidifex metallicus, and Sulfurisphaera ohwakuensis.	Counts JA, Vitko NP, Kelly RM.	Microbiol Resour Announc	10.1128/mra.01490-19	2020
Metabolism	Tetrathionate hydrolase from the acidophilic microorganisms.	Kanao T.	Front Microbiol	10.3389/fmicb.2024.1338669	2024
Metabolism	The Two-Component System RsrS-RsrR Regulates the Tetrathionate Intermediate Pathway for Thiosulfate Oxidation in Acidithiobacillus caldus.	Wang ZB, Li YQ, Lin JQ, Pang X, Liu XM, Liu BQ, Wang R, Zhang CJ, Wu Y, Lin JQ, Chen LX.	Front Microbiol	10.3389/fmicb.2016.01755	2016
	Highly integrated adaptive mechanisms in Spiribacter halalkaliphilus, a bacterium abundant in Chinese soda-saline lakes.	Xue Q, Zhao D, Zhang S, Zhou H, Zuo Z, Zhou J, Li M, Xiang H.	Environ Microbiol	10.1111/1462-2920.15794	2021
	Sulfur Oxidation in the Acidophilic Autotrophic Acidithiobacillus spp.	Wang R, Lin JQ, Liu XM, Pang X, Zhang CJ, Yang CL, Gao XY, Lin CM, Li YQ, Li Y, Lin JQ, Chen LX.	Front Microbiol	10.3389/fmicb.2018.03290	2018
Metabolism	An Extracellular Tetrathionate Hydrolase from the Thermoacidophilic Archaeon Acidianus Ambivalens with an Activity Optimum at pH 1.	Protze J, Muller F, Lauber K, Nass B, Mentele R, Lottspeich F, Kletzin A.	Front Microbiol	10.3389/fmicb.2011.00068	2011
Metabolism	The sulphur oxygenase reductase from Acidianus ambivalens is a multimeric protein containing a low-potential mononuclear non-haem iron centre.	Urich T, Bandeiras TM, Leal SS, Rachel R, Albrecht T, Zimmermann P, Scholz C, Teixeira M, Gomes CM, Kletzin A.	Biochem J	10.1042/bj20040003	2004
Metabolism	Protein-protein, protein-RNA and protein-lipid interactions of signal-recognition particle components in the hyperthermoacidophilic archaeon Acidianus ambivalens.	Moll RG.	Biochem J	10.1042/bj20030475	2003
	Molecular analysis of pDL10 from Acidianus ambivalens reveals a family of related plasmids from extremely thermophilic and acidophilic archaea.	Kletzin A, Lieke A, Urich T, Charlebois RL, Sensen CW.	Genetics	10.1093/genetics/152.4.1307	1999
Metabolism	Microbial alkyl- and aryl-sulfatases: mechanism, occurrence, screening and stereoselectivities.	Toesch M, Schober M, Faber K.	Appl Microbiol Biotechnol	10.1007/s00253-013-5438-0	2014
	Organic solutes in hyperthermophilic archaea.	Martins LO, Huber R, Huber H, Stetter KO, Da Costa MS, Santos H.	Appl Environ Microbiol	10.1128/aem.63.3.896-902.1997	1997
Metabolism	Molecular characterization of the sor gene, which encodes the sulfur oxygenase/reductase of the thermoacidophilic Archaeum Desulfurolobus ambivalens.	Kletzin A.	J Bacteriol	10.1128/jb.174.18.5854-5859.1992	1992
Phylogeny	A novel acidophilic, thermophilic iron and sulfur-oxidizing archaeon isolated from a hot spring of tengchong, yunnan, China.	Ding J, Zhang R, Yu Y, Jin D, Liang C, Yi Y, Zhu W, Xia J.	Braz J Microbiol	10.1590/s1517-838220110002000016	2011
Genetics	Phenotype-driven assessment of the ancestral trajectory of sulfur biooxidation in the thermoacidophilic archaea Sulfolobaceae.	Willard DJ, H Manesh MJ, Bing RG, Alexander BH, Kelly RM.	mBio	10.1128/mbio.01033-24	2024
Enzymology	Enzymes Catalyzing Crotonyl-CoA Conversion to Acetoacetyl-CoA During the Autotrophic CO₂ Fixation in Metallosphaera sedula.	Liu L, Huber H, Berg IA.	Front Microbiol	10.3389/fmicb.2020.00354	2020
Genetics	Draft Genome Sequence of Acidianus ambivalens DSM 3772, an Aerobic Thermoacidophilic Sulfur Disproportionator.	Bertran E, Ward LM, Johnston DT	Microbiol Resour Announc	10.1128/MRA.01415-19	2020
Enzymology	Functional characterization of an extremely thermophilic ATPase in membranes of the crenarchaeon Acidianus ambivalens.	Hinrichs M, Schafer G, Anemuller S	Biol Chem	10.1515/BC.1999.132	1999
Enzymology	The terminal quinol oxidase of the hyperthermophilic archaeon Acidianus ambivalens exhibits a novel subunit structure and gene organization.	Purschke WG, Schmidt CL, Petersen A, Schafer G	J Bacteriol	10.1128/jb.179.4.1344-1353.1997	1997
Genetics	A seven-iron ferredoxin from the thermoacidophilic archaeon Desulfurolobus ambivalens.	Teixeira M, Batista R, Campos AP, Gomes C, Mendes J, Pacheco I, Anemuller S, Hagen WR	Eur J Biochem	10.1111/j.1432-1033.1995.tb20392.x	1995
Enzymology	Coupled enzymatic production of sulfite, thiosulfate, and hydrogen sulfide from sulfur: purification and properties of a sulfur oxygenase reductase from the facultatively anaerobic archaebacterium Desulfurolobus ambivalens.	Kletzin A	J Bacteriol	10.1128/jb.171.3.1638-1643.1989	1989

References

#1468	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 3772
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Acidianus ambivalens (DSM 3772, JCM 9191, LEI 10)

Name and taxonomic classification

Morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_009729015

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media