Strain identifier

BacDive ID: 16643

Type strain: Yes

Species: Acidianus ambivalens

Strain history: DSM 3772 <-- W. Zillig Lei 10.

NCBI tax ID(s): 2283 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 1468

BacDive-ID: 16643

DSM-Number: 3772

keywords: genome sequence, 16S sequence, Archaea, anaerobe, hyperthermophilic

description: Acidianus ambivalens DSM 3772 is an anaerobe, hyperthermophilic archaeon that was isolated from solfataric mud.

NCBI tax id

  • NCBI tax id: 2283
  • Matching level: species

strain history

@refhistory
1468<- W. Zillig, Max Planck Inst. Biochem., Martinsried, Germany; LEI 10 <- W. Zillig {1984}
67770DSM 3772 <-- W. Zillig Lei 10.

doi: 10.13145/bacdive16643.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/thermoproteota
  • domain: Archaea
  • phylum: Thermoproteota
  • class: Thermoprotei
  • order: Sulfolobales
  • family: Sulfolobaceae
  • genus: Acidianus
  • species: Acidianus ambivalens
  • full scientific name: Acidianus ambivalens (Zillig and Böck 1987) Fuchs et al. 1996
  • synonyms

    • @ref: 20215
    • synonym: Desulfurolobus ambivalens

@ref: 1468

domain: Archaea

phylum: Crenarchaeota

class: Thermoprotei

order: Sulfolobales

family: Sulfolobaceae

genus: Acidianus

species: Acidianus ambivalens

full scientific name: Acidianus ambivalens (Zillig and Böck 1987) Fuchs et al. 1996

type strain: yes

Morphology

colony morphology

  • @ref: 1468
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

  • @ref: 1468
  • name: ACIDIANUS MEDIUM (ANAEROBIC) (DSMZ Medium 358a)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/358a
  • composition: Name: ACIDIANUS MEDIUM (ANAEROBIC) (DSMZ Medium 358a; with strain-specific modifications) Composition: Sulfur 4.9505 g/l (NH4)2SO4 1.28713 g/l KH2PO4 0.277228 g/l MgSO4 x 7 H2O 0.247525 g/l CaCl2 x 2 H2O 0.0693069 g/l Yeast extract 0.019802 g/l FeCl3 x 6 H2O 0.019802 g/l Na2B4O7 x 10 H2O 0.00445545 g/l MnCl2 x 4 H2O 0.00178218 g/l ZnSO4 x 7 H2O 0.000217822 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Na2MoO4 x 2 H2O 2.9703e-05 g/l VOSO4 x 2 H2O 2.9703e-05 g/l CoSO4 x 7 H2O 9.90099e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
1468positivegrowth80hyperthermophilic
67770positivegrowth80hyperthermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 1468
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
1468solfataric mudLeihnukur fissureIcelandISLEurope
67770Solfataric waterhole at Leirhukur fissureIcelandISLEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Mud (Sludge)
#Environmental#Terrestrial#Volcanic

Safety information

risk assessment

  • @ref: 1468
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218D.ambivalens DNA for 16S ribosomal RNA geneX904841422ena2283
1468Acidianus ambivalens 16S rRNA gene, almost full-length sequenceD855061462ena2283
67770Desulfurolobus ambivalens 16S rRNA geneD38774860ena2283

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
67770Acidianus ambivalens LEI 10GCA_009729015completencbi2283
67770Acidianus ambivalens DSM 3772GCA_009428885contigncbi2283

GC content

  • @ref: 1468
  • GC-content: 32.7

External links

@ref: 1468

culture collection no.: DSM 3772, JCM 9191, LEI 10

straininfo link

  • @ref: 85664
  • straininfo: 47758

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology2493451Coupled enzymatic production of sulfite, thiosulfate, and hydrogen sulfide from sulfur: purification and properties of a sulfur oxygenase reductase from the facultatively anaerobic archaebacterium Desulfurolobus ambivalens.Kletzin AJ Bacteriol10.1128/jb.171.3.1638-1643.19891989Archaea/*enzymology, *Archaeal Proteins, Bacteria/*enzymology, Bacteria, Anaerobic/enzymology, Hydrogen Sulfide/analysis, Kinetics, Oxidoreductases/*isolation & purification/metabolism, Oxidoreductases Acting on Sulfur Group Donors, Sulfites/analysis, Thermodynamics, Thiosulfates/analysisBiotechnology
Genetics7851403A seven-iron ferredoxin from the thermoacidophilic archaeon Desulfurolobus ambivalens.Teixeira M, Batista R, Campos AP, Gomes C, Mendes J, Pacheco I, Anemuller S, Hagen WREur J Biochem10.1111/j.1432-1033.1995.tb20392.x1995Amino Acid Sequence, Amino Acids/analysis, Archaea/*chemistry, Electron Spin Resonance Spectroscopy, Ferredoxins/*chemistry/isolation & purification/physiology, Iron/*analysis, Molecular Sequence Data, Sequence Homology, Amino Acid, Spectrophotometry, UltravioletEnzymology
Enzymology9023221The terminal quinol oxidase of the hyperthermophilic archaeon Acidianus ambivalens exhibits a novel subunit structure and gene organization.Purschke WG, Schmidt CL, Petersen A, Schafer GJ Bacteriol10.1128/jb.179.4.1344-1353.19971997Amino Acid Sequence, Chromosome Mapping, Cloning, Molecular, *Genes, Bacterial, Molecular Sequence Data, Multigene Family, Open Reading Frames, *Operon, Oxidation-Reduction, Oxidoreductases/*chemistry/*genetics/isolation & purification/metabolism, Phylogeny, Point Mutation, Protein Conformation, Protein Structure, Secondary, RNA, Bacterial/genetics, RNA, Messenger/genetics, Sequence Alignment, Sulfolobaceae/*enzymology/genetics, Thiophenes/metabolism, Transcription, GeneticPhylogeny
Enzymology10543443Functional characterization of an extremely thermophilic ATPase in membranes of the crenarchaeon Acidianus ambivalens.Hinrichs M, Schafer G, Anemuller SBiol Chem10.1515/BC.1999.1321999Adenosine Triphosphatases/*metabolism, Archaea/*enzymology, Catalysis, Cations, Divalent, Cell Membrane/enzymology, Hydrogen-Ion Concentration, Hydrolysis, Kinetics, Substrate SpecificityMetabolism
Genetics31948968Draft Genome Sequence of Acidianus ambivalens DSM 3772, an Aerobic Thermoacidophilic Sulfur Disproportionator.Bertran E, Ward LM, Johnston DTMicrobiol Resour Announc10.1128/MRA.01415-192020

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1468Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3772)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3772
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
85664Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47758.1StrainInfo: A central database for resolving microbial strain identifiers