Sulfurisphaera ohwakuensis (DSM 12421, IFO 15161, JCM 9065)

Name: Sulfurisphaera ohwakuensis (DSM 12421, IFO 15161, JCM 9065)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16639

Type strain

Strain Designation TA-1

Culture col. no. DSM 12421 IFO 15161 JCM 9065 NBRC 15161 TA-1

NCBI tax ID(s) 69656

Special Collections DSMZ JCM

History DSMZ <- JCM <- IFO <- N. Kurosawa, Soka University, Faculty of Engineeering, Tokyo, Japan; TA-1 JCM <-- IFO 15161 <-- T. Itoh TA-1.

Links

Sulfurisphaera ohwakuensis TA-1 is an archaeon that was isolated from hot spring.

genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 49285

DSM 12421,IFO 15161,JCM 9065,NBRC 15161

@ref 20215

Last LPSN update 2026-05-29 08:28:49

Domain Archaea

Phylum Thermoproteota

Class Thermoprotei

Order Sulfolobales

Family Sulfolobaceae

Genus Sulfurisphaera

Species Sulfurisphaera ohwakuensis

Full scientific name Sulfurisphaera ohwakuensis Kurosawa et al. 1998

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
4552	SULFOLOBUS MEDIUM (DSMZ Medium 88)		Medium recipe at MediaDive	Name: SULFOLOBUS MEDIUM (DSMZ Medium 88) Composition: (NH4)2SO4 1.28713 g/l Yeast extract 0.990099 g/l KH2PO4 0.277228 g/l MgSO4 x 7 H2O 0.247525 g/l CaCl2 x 2 H2O 0.0693069 g/l FeCl3 x 6 H2O 0.019802 g/l Na2B4O7 x 10 H2O 0.00445545 g/l MnCl2 x 4 H2O 0.00178218 g/l ZnSO4 x 7 H2O 0.000217822 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Na2MoO4 x 2 H2O 2.9703e-05 g/l VOSO4 x 2 H2O 2.9703e-05 g/l CoSO4 x 7 H2O 9.90099e-06 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
4552	positive	growth	75
67770	positive	growth	75

Physiology and metabolism

Spore formation

@ref	Spore formation	Confidence
125439		90.354

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
4552	hot spring	Ohwaku Valley, Hakone	Japan	JPN	Asia
67770	Acidic hot mud	Hakone	Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence D85507 (>99% sequence identity) for Sulfurisphaera from Microbeatlas

Aquatic Samples	24
Soil Samples	0
Animal Samples	1
Plant Samples	0
Total Samples	25

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
4552	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM972905v1 assembly for Sulfurisphaera ohwakuensis TA-1	complete	GCA_009729055	69656.6	8048261668	69656	95.57
66792	ASM1420210v1 assembly for Sulfurisphaera ohwakuensis DSM 12421	contig	GCA_014202105	69656.7	2861352127	69656	61.57

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
4552	Sulfurisphaera ohwakuensis 16S rRNA gene, complete sequence	D85507	2213		69656

GC content

@ref	GC-content (mol%)	Method
4552	33	high performance liquid chromatography (HPLC)
67770	32.9	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1420210v1 assembly for Sulfurisphaera ohwakuensis DSM 12421
NCBI: GCA_014202105

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	gram_stain	BacteriaNetⓘ	negative	73.13	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	66.10	no
125439	motility	BacteriaNetⓘ	no	67.64	no
125439	spore_formation	BacteriaNetⓘ	no	90.35	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Sulfurisphaera ohwakuensis TA-1
NCBI: GCA_009729055.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	73.62	no
125438	anaerobic	anaerobicⓘ	yes	70.80	no
125438	aerobic	aerobicⓘ	no	69.37	no
125438	spore-forming	spore-formingⓘ	no	87.49	no
125438	thermophilic	thermophileⓘ	yes	81.91	no
125438	flagellated	motile2+ⓘ	no	85.88	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	pH Threshold Impacts Chalcopyrite Bioleaching Dynamics for the Extreme Thermoacidophile Sulfurisphaera ohwakuensis.	Willard DJ, Manesh MJH, John KM, Kelly RM.	Biotechnol Bioeng	10.1002/bit.28945	2025
Genetics	Chalcopyrite bioleaching efficacy by extremely thermoacidophilic archaea leverages balanced iron and sulfur biooxidation.	Manesh MJH, Willard DJ, John KM, Kelly RM.	Bioresour Technol	10.1016/j.biortech.2024.131198	2024
	Genome Sequences of Five Type Strain Members of the Archaeal Family Sulfolobaceae, Acidianus ambivalens, Acidianus infernus, Stygiolobus azoricus, Sulfuracidifex metallicus, and Sulfurisphaera ohwakuensis.	Counts JA, Vitko NP, Kelly RM.	Microbiol Resour Announc	10.1128/mra.01490-19	2020
Metabolism	Microbial alkyl- and aryl-sulfatases: mechanism, occurrence, screening and stereoselectivities.	Toesch M, Schober M, Faber K.	Appl Microbiol Biotechnol	10.1007/s00253-013-5438-0	2014
Phylogeny	Sulfurisphaera javensis sp. nov., a hyperthermophilic and acidophilic archaeon isolated from Indonesian hot spring, and reclassification of Sulfolobus tokodaii Suzuki et al. 2002 as Sulfurisphaera tokodaii comb. nov.	Tsuboi K, Sakai HD, Nur N, Stedman KM, Kurosawa N, Suwanto A	Int J Syst Evol Microbiol	10.1099/ijsem.0.002765	2018
Phylogeny	Sulfurisphaera ohwakuensis gen. nov., sp. nov., a novel extremely thermophilic acidophile of the order Sulfolobales.	Kurosawa N, Itoh YH, Iwai T, Sugai A, Uda I, Kimura N, Horiuchi T, Itoh T	Int J Syst Bacteriol	10.1099/00207713-48-2-451	1998

References

#4552	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12421
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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About us

Sulfurisphaera ohwakuensis (DSM 12421, IFO 15161, JCM 9065)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_009729055

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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