Strain identifier

BacDive ID: 16639

Type strain: Yes

Species: Sulfurisphaera ohwakuensis

Strain Designation: TA-1

Strain history: IFO 15161 <-- T. Itoh TA-1.

NCBI tax ID(s): 69656 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 4552

BacDive-ID: 16639

DSM-Number: 12421

keywords: genome sequence, 16S sequence, Archaea, anaerobe, spore-forming, thermophilic, Gram-negative

description: Sulfurisphaera ohwakuensis TA-1 is an anaerobe, spore-forming, thermophilic archaeon that was isolated from hot spring.

NCBI tax id

  • NCBI tax id: 69656
  • Matching level: species

strain history

@refhistory
4552<- JCM <- IFO <- N. Kurosawa, Soka University, Faculty of Engineeering, Tokyo, Japan; TA-1
67770IFO 15161 <-- T. Itoh TA-1.

doi: 10.13145/bacdive16639.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/thermoproteota
  • domain: Archaea
  • phylum: Thermoproteota
  • class: Thermoprotei
  • order: Sulfolobales
  • family: Sulfolobaceae
  • genus: Sulfurisphaera
  • species: Sulfurisphaera ohwakuensis
  • full scientific name: Sulfurisphaera ohwakuensis Kurosawa et al. 1998

@ref: 4552

domain: Archaea

phylum: Crenarchaeota

class: Thermoprotei

order: Sulfolobales

family: Sulfolobaceae

genus: Sulfurisphaera

species: Sulfurisphaera ohwakuensis

full scientific name: Sulfurisphaera ohwakuensis Kurosawa et al. 1998 emend. Tsuboi et al. 2018

strain designation: TA-1

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 99.902

Culture and growth conditions

culture medium

  • @ref: 4552
  • name: SULFOLOBUS MEDIUM (DSMZ Medium 88)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/88
  • composition: Name: SULFOLOBUS MEDIUM (DSMZ Medium 88) Composition: (NH4)2SO4 1.28713 g/l Yeast extract 0.990099 g/l KH2PO4 0.277228 g/l MgSO4 x 7 H2O 0.247525 g/l CaCl2 x 2 H2O 0.0693069 g/l FeCl3 x 6 H2O 0.019802 g/l Na2B4O7 x 10 H2O 0.00445545 g/l MnCl2 x 4 H2O 0.00178218 g/l ZnSO4 x 7 H2O 0.000217822 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Na2MoO4 x 2 H2O 2.9703e-05 g/l VOSO4 x 2 H2O 2.9703e-05 g/l CoSO4 x 7 H2O 9.90099e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerangeconfidence
4552positivegrowth75thermophilic
67770positivegrowth75thermophilic
69480thermophilic100

Physiology and metabolism

oxygen tolerance

  • @ref: 69480
  • oxygen tolerance: anaerobe
  • confidence: 98.196

spore formation

@refspore formationconfidence
69481yes100
69480no99.879

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
4552hot springOhwaku Valley, HakoneJapanJPNAsia
67770Acidic hot mudHakoneJapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_187290.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17922;96_68912;97_89562;98_121853;99_187290&stattab=map
  • Last taxonomy: Sulfurisphaera
  • 16S sequence: D85507
  • Sequence Identity:
  • Total samples: 25
  • aquatic counts: 24
  • animal counts: 1

Safety information

risk assessment

  • @ref: 4552
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
4552Sulfurisphaera ohwakuensis 16S rRNA gene, complete sequenceD855072213ena69656
67770Acidianus sp. 16S rRNA geneD38775859ena41979

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Sulfurisphaera ohwakuensis TA-1GCA_009729055completencbi69656
66792Sulfurisphaera ohwakuensis DSM 12421GCA_014202105contigncbi69656
66792Sulfurisphaera ohwakuensis DSM 124212861352127draftimg69656

GC content

@refGC-contentmethod
455233high performance liquid chromatography (HPLC)
6777032.9high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno77.934no
flagellatedno97.388no
gram-positiveno94.052no
anaerobicyes85.724no
aerobicno85.792no
halophileno77.828no
spore-formingno95.196no
thermophileyes100no
glucose-utilyes81.833no
glucose-fermentno76.285no

External links

@ref: 4552

culture collection no.: DSM 12421, IFO 15161, JCM 9065, NBRC 15161

straininfo link

  • @ref: 85660
  • straininfo: 49285

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny9731283Sulfurisphaera ohwakuensis gen. nov., sp. nov., a novel extremely thermophilic acidophile of the order Sulfolobales.Kurosawa N, Itoh YH, Iwai T, Sugai A, Uda I, Kimura N, Horiuchi T, Itoh TInt J Syst Bacteriol10.1099/00207713-48-2-4511998Base Composition, Base Sequence, DNA, Bacterial, Fresh Water/microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Bacterial/analysis, RNA, Ribosomal, 16S/analysis, Sulfolobales/*classification/genetics/ultrastructureGenetics
Phylogeny29671720Sulfurisphaera javensis sp. nov., a hyperthermophilic and acidophilic archaeon isolated from Indonesian hot spring, and reclassification of Sulfolobus tokodaii Suzuki et al. 2002 as Sulfurisphaera tokodaii comb. nov.Tsuboi K, Sakai HD, Nur N, Stedman KM, Kurosawa N, Suwanto AInt J Syst Evol Microbiol10.1099/ijsem.0.0027652018Base Composition, Chemoautotrophic Growth, DNA, Archaeal/genetics, Hot Springs/*microbiology, Indonesia, Lipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sulfolobaceae/*classification/genetics/isolation & purification, SulfolobusTranscriptome

Reference

@idauthorscataloguedoi/urltitle
4552Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12421)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12421
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
85660Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49285.1StrainInfo: A central database for resolving microbial strain identifiers