Strain identifier
BacDive ID: 16639
Type strain: 
Species: Sulfurisphaera ohwakuensis
Strain Designation: TA-1
Strain history: IFO 15161 <-- T. Itoh TA-1.
NCBI tax ID(s): 69656 (species)
version 9.1 (current version)
General
@ref: 4552
BacDive-ID: 16639
DSM-Number: 12421
keywords: genome sequence, 16S sequence, Archaea, spore-forming, Gram-negative
description: Sulfurisphaera ohwakuensis TA-1 is a spore-forming, Gram-negative archaeon that was isolated from hot spring.
NCBI tax id
- NCBI tax id: 69656
- Matching level: species
strain history
| @ref | history |
|---|---|
| 4552 | <- JCM <- IFO <- N. Kurosawa, Soka University, Faculty of Engineeering, Tokyo, Japan; TA-1 |
| 67770 | IFO 15161 <-- T. Itoh TA-1. |
doi: 10.13145/bacdive16639.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/thermoproteota
- domain: Archaea
- phylum: Thermoproteota
- class: Thermoprotei
- order: Sulfolobales
- family: Sulfolobaceae
- genus: Sulfurisphaera
- species: Sulfurisphaera ohwakuensis
- full scientific name: Sulfurisphaera ohwakuensis Kurosawa et al. 1998
@ref: 4552
domain: Archaea
phylum: Crenarchaeota
class: Thermoprotei
order: Sulfolobales
family: Sulfolobaceae
genus: Sulfurisphaera
species: Sulfurisphaera ohwakuensis
full scientific name: Sulfurisphaera ohwakuensis Kurosawa et al. 1998 emend. Tsuboi et al. 2018
strain designation: TA-1
type strain: yes
Culture and growth conditions
culture medium
- @ref: 4552
- name: SULFOLOBUS MEDIUM (DSMZ Medium 88)
- growth: yes
- link: https://mediadive.dsmz.de/medium/88
- composition: Name: SULFOLOBUS MEDIUM (DSMZ Medium 88) Composition: (NH4)2SO4 1.28713 g/l Yeast extract 0.990099 g/l KH2PO4 0.277228 g/l MgSO4 x 7 H2O 0.247525 g/l CaCl2 x 2 H2O 0.0693069 g/l FeCl3 x 6 H2O 0.019802 g/l Na2B4O7 x 10 H2O 0.00445545 g/l MnCl2 x 4 H2O 0.00178218 g/l ZnSO4 x 7 H2O 0.000217822 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Na2MoO4 x 2 H2O 2.9703e-05 g/l VOSO4 x 2 H2O 2.9703e-05 g/l CoSO4 x 7 H2O 9.90099e-06 g/l Distilled water
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 4552 | positive | growth | 75 |
| 67770 | positive | growth | 75 |
Physiology and metabolism
spore formation
- @ref: 69481
- spore formation: yes
- confidence: 100
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 4552 | hot spring | Ohwaku Valley, Hakone | Japan | JPN | Asia |
| 67770 | Acidic hot mud | Hakone | Japan | JPN | Asia |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Thermal spring |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_187290.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17922;96_68912;97_89562;98_121853;99_187290&stattab=map
- Last taxonomy: Sulfurisphaera
- 16S sequence: D85507
- Sequence Identity:
- Total samples: 25
- aquatic counts: 24
- animal counts: 1
Safety information
risk assessment
- @ref: 4552
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 4552
- description: Sulfurisphaera ohwakuensis 16S rRNA gene, complete sequence
- accession: D85507
- length: 2213
- database: nuccore
- NCBI tax ID: 69656
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Sulfurisphaera ohwakuensis TA-1 | GCA_009729055 | complete | ncbi | 69656 |
| 66792 | Sulfurisphaera ohwakuensis DSM 12421 | GCA_014202105 | contig | ncbi | 69656 |
| 66792 | Sulfurisphaera ohwakuensis DSM 12421 | 2861352127 | draft | img | 69656 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 4552 | 33 | high performance liquid chromatography (HPLC) |
| 67770 | 32.9 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69481 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 100 | no |
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 73.619 | no |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | yes | 70.801 | no |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | no | 69.366 | no |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 87.489 | no |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | yes | 81.911 | no |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | no | 85.875 | no |
External links
@ref: 4552
culture collection no.: DSM 12421, IFO 15161, JCM 9065, NBRC 15161
straininfo link
- @ref: 85660
- straininfo: 49285
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 9731283 | Sulfurisphaera ohwakuensis gen. nov., sp. nov., a novel extremely thermophilic acidophile of the order Sulfolobales. | Kurosawa N, Itoh YH, Iwai T, Sugai A, Uda I, Kimura N, Horiuchi T, Itoh T | Int J Syst Bacteriol | 10.1099/00207713-48-2-451 | 1998 | Base Composition, Base Sequence, DNA, Bacterial, Fresh Water/microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Bacterial/analysis, RNA, Ribosomal, 16S/analysis, Sulfolobales/*classification/genetics/ultrastructure | Genetics |
| Phylogeny | 29671720 | Sulfurisphaera javensis sp. nov., a hyperthermophilic and acidophilic archaeon isolated from Indonesian hot spring, and reclassification of Sulfolobus tokodaii Suzuki et al. 2002 as Sulfurisphaera tokodaii comb. nov. | Tsuboi K, Sakai HD, Nur N, Stedman KM, Kurosawa N, Suwanto A | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002765 | 2018 | Base Composition, Chemoautotrophic Growth, DNA, Archaeal/genetics, Hot Springs/*microbiology, Indonesia, Lipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sulfolobaceae/*classification/genetics/isolation & purification, Sulfolobus | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 4552 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12421) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-12421 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |
| 85660 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID49285.1 | StrainInfo: A central database for resolving microbial strain identifiers |