Name: Methylolobus aquaticus JCM 33786
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 164650

Type strain:

Species: Methylolobus aquaticus

Culture col. no.: JCM 33786, KCTC 72733, MCC 4198, FWC 3

Strain history: M. C. Rahalkar; Agharkar Res. Inst., India; FWC3.

NCBI tax ID(s): 2516328 (species)

Special Collections

Section

Methylolobus aquaticus JCM 33786 is an aerobe, Gram-negative, coccus-shaped bacterium that was isolated from Tropical freshwater wetland in Western India.

coccus-shaped
Gram-negative
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Methylococcales*
	Family	*Methylococcaceae*
	Genus	"Methylolobus"
	Species	"Methylolobus aquaticus"
	Full Scientific Name (LPSN)	Methylolobus aquaticus Rahalkar et al. 2020

Information on morphological and physiological properties Morphology

[Ref.: #68101]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %98.145

[Ref.: #68101]	Cell length	1.2-1.5 µm

[Ref.: #68101]	Cell width	1.0-1.2 µm

[Ref.: #68101]	Cell shape	coccus-shaped

[Ref.: #68101]	Motility	no

[Ref.: #68101]	Colony color	flesh pink

[Ref.: #68101]	Cultivation medium used	NMS agarose (2% agarose)

Information on culture and growth conditions Culture and growth conditions

	Temperatures	Kind of temperature		Temperature
[Ref.: #67770]			growth	25 °C
[Ref.: #68101]			growth	25-35 °C

	pH	Kind of pH		pH
[Ref.: #68101]			growth	5.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #68101]

Oxygen tolerance

aerobe

[Ref.: #69480]

Ability of spore formation

no Confidence in %95.184

[Ref.: #68101]	Observation	reproduce by binary fission
[Ref.: #68101]	Observation	occur singly, in pairs, triads, tetracocci or aggregates
[Ref.: #68101]	Observation	Obligate methanotroph
[Ref.: #68101]	Observation	Methanol supports growth from 0.02 to 5%
[Ref.: #68101]	Observation	Can be cryo-preserved using 5% DMSO at - 80 C

	Enzymes	Enzyme	Enzyme activity
[Ref.: #68101]		nitrogenase	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Tropical freshwater wetland in Western India

[Ref.: #68101]	Sample type/isolated from	shallow freshwater patch of a wetland which was the end of a freshwater creek region
[Ref.: #68101]	Sampling date	2017-09
[Ref.: #68101]	Geographic location (country and/or sea, region)	near Nagaon beach, Alibag
[Ref.: #68101]	Country	India
[Ref.: #68101]	Country ISO 3 Code	IND
[Ref.: #68101]	Continent	Asia
[Ref.: #68101]	Geographic location	18.4417°/72.9056°
* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Methylolobus aquaticus strain FWC3 16S ribosomal RNA gene, partial sequence	MN080433	1527	GenBank ENA	1933926 tax ID
[Ref.: #68101]	Methylolobus aquaticus strain FWC3 16S ribosomal RNA gene, partial sequence	MN080433.1	1527	GenBank ENA	1933926 tax ID
[Ref.: #67770]	Methylolobus aquaticus strain FWC3 16S ribosomal RNA gene, partial sequence	MH789551	1481	GenBank ENA	1933926 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #68101]	Methylococcaceae bacterium FWC3	GCA_004168015	scaffold	GenBank ENA	2516328 tax ID

[Ref.: #67770]

GC-content

63 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methylococcaceae bacterium FWC3 FWC3 NCBI: GCA_004168015.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	98.145	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	83.327	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	58.588	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	95.184	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	92.092	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	79.931	yes

Availability in culture collections External links

[Ref.: #67770]

Culture collection no.

JCM 33786, KCTC 72733, MCC 4198, FWC 3

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68101

Monali C. Rahalkar, Kumal Khatri, Jyoti Mohite, Pranitha S. Pandit, Rahul A. Bahulikar: A novel Type I methanotroph Methylolobus aquaticus gen. nov. sp. nov. isolated from a tropical wetland. Antonie Van Leeuwenhoek 113: 959 - 971 2020 ( DOI 10.1007/s10482-020-01410-9 )

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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Methylococcaceae bacterium FWC3 FWC3

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