Cite this strain

"Methylolobus aquaticus" (JCM 33786, KCTC 72733, MCC 4198)

BacDive ID 164650

Type strain

Culture col. no. JCM 33786 KCTC 72733 MCC 4198 FWC 3

NCBI tax ID(s) 2516328

Special Collections JCM KCTC Literature strains

History JCM <-- M. C. Rahalkar; Agharkar Res. Inst., India; FWC3.

Links

"Methylolobus aquaticus" JCM 33786 is an aerobe, Gram-negative, coccus-shaped bacterium that was isolated from Tropical freshwater wetland in Western India.

Gram-negative coccus-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 26202

KCTC 72733,JCM-33786

@ref 20215

Last LPSN update 2026-05-29 08:45:28

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Methylococcales

Family Methylococcaceae

Genus "Methylolobus"

Species "Methylolobus aquaticus"

Full scientific name Methylolobus aquaticus Rahalkar et al. 2020

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Confidence
68101	negative	1.2-1.5 µm	1.0-1.2 µm	coccus-shaped
125438	negative					98.145

Colony morphology

@ref	Colony color	Medium used
68101	flesh pink
68101		NMS agarose (2% agarose)

Culture and growth conditions

Culture temp

@ref	Growth	Type	Temperature (°C)
67770	positive	growth	25
68101	positive	growth	25-35

Culture pH

@ref	Ability	Type	PH
68101	positive	growth	5.0-8.0

Physiology and metabolism

Oxygen tolerance

68101

Oxygen toleranceaerobe

Spore formation

@ref	Spore formation	Confidence
125438		95.184

Observation

@ref	Observation
68101	reproduce by binary fission
68101	occur singly, in pairs, triads, tetracocci or aggregates
68101	Obligate methanotroph
68101	Methanol supports growth from 0.02 to 5%
68101	Can be cryo-preserved using 5% DMSO at - 80 C

Enzymes

@ref	Value	Activity
68101	nitrogenase	+

Isolation, sampling and environmental information

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
67770	Tropical freshwater wetland in Western India
68101	shallow freshwater patch of a wetland which was the end of a freshwater creek region	2017-09	near Nagaon beach, Alibag	India	IND	Asia	18.4417	72.9056 18.4417/72.9056

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
68101	ASM416801v1 assembly for Methylolobus aquaticus FWC3	scaffold	GCA_004168015	2516328.3	3000381075	2516328	26.69

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
67770	Methylolobus aquaticus strain FWC3 16S ribosomal RNA gene, partial sequence	MH789551	1481	1933926
67770	Methylolobus aquaticus strain FWC3 16S ribosomal RNA gene, partial sequence	MN080433	1527	1933926
68101	Methylolobus aquaticus strain FWC3 16S ribosomal RNA gene, partial sequence	MN080433.1	1527	1933926

GC content

@ref	GC-content (mol%)	Method
67770	63	genome sequence analysis

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methylococcaceae bacterium FWC3 FWC3
NCBI: GCA_004168015.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.15	yes
125438	anaerobic	anaerobicⓘ	no	83.33	yes
125438	aerobic	aerobicⓘ	yes	58.59	no
125438	spore-forming	spore-formingⓘ	no	95.18	no
125438	thermophilic	thermophileⓘ	no	92.09	yes
125438	flagellated	motile2+ⓘ	no	79.93	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	A novel Type I methanotroph Methylolobus aquaticus gen. nov. sp. nov. isolated from a tropical wetland.	Rahalkar MC, Khatri K, Mohite J, Pandit PS, Bahulikar RA.	Antonie Van Leeuwenhoek	10.1007/s10482-020-01410-9	2020

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68101	Monali C. Rahalkar, Kumal Khatri, Jyoti Mohite, Pranitha S. Pandit, Rahul A. Bahulikar: A novel Type I methanotroph Methylolobus aquaticus gen. nov. sp. nov. isolated from a tropical wetland. Antonie Van Leeuwenhoek 113: 959 - 971 2020 ( DOI 10.1007/s10482-020-01410-9 )
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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"Methylolobus aquaticus" (JCM 33786, KCTC 72733, MCC 4198)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Enzymes

Isolation, sampling and environmental information

Isolation

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_004168015

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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