Strain identifier
BacDive ID: 164650
Type strain: 
Species: Methylolobus aquaticus
Strain history: M. C. Rahalkar; Agharkar Res. Inst., India; FWC3.
General
@ref: 67770
BacDive-ID: 164650
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, coccus-shaped
description: Methylolobus aquaticus JCM 33786 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from Tropical freshwater wetland in Western India.
strain history
- @ref: 67770
- history: M. C. Rahalkar; Agharkar Res. Inst., India; FWC3.
doi: 10.13145/bacdive164650.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Methylococcales
- family: Methylococcaceae
- genus: Methylolobus
- species: Methylolobus aquaticus
- full scientific name: Methylolobus aquaticus Rahalkar et al. 2020
@ref: 67770
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Methylococcales
family: Methylococcaceae
genus: Methylolobus
species: Methylolobus aquaticus
full scientific name: Methylolobus aquaticus
type strain: no
Morphology
cell morphology
- @ref: 68101
- gram stain: negative
- cell length: 1.2-1.5 µm
- cell width: 1.0-1.2 µm
- cell shape: coccus-shaped
- motility: no
colony morphology
| @ref | colony color | medium used |
|---|---|---|
| 68101 | flesh pink | |
| 68101 | NMS agarose (2% agarose) |
Culture and growth conditions
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 67770 | positive | growth | 25 | mesophilic |
| 68101 | positive | growth | 25-35 | mesophilic |
culture pH
- @ref: 68101
- ability: positive
- type: growth
- pH: 5.0-8.0
Physiology and metabolism
oxygen tolerance
- @ref: 68101
- oxygen tolerance: aerobe
observation
| @ref | observation |
|---|---|
| 68101 | reproduce by binary fission |
| 68101 | occur singly, in pairs, triads, tetracocci or aggregates |
| 68101 | Obligate methanotroph |
| 68101 | Methanol supports growth from 0.02 to 5% |
| 68101 | Can be cryo-preserved using 5% DMSO at - 80 C |
enzymes
- @ref: 68101
- value: nitrogenase
- activity: +
Isolation, sampling and environmental information
isolation
| @ref | sample type | sampling date | geographic location | country | origin.country | continent | latitude | longitude |
|---|---|---|---|---|---|---|---|---|
| 67770 | Tropical freshwater wetland in Western India | |||||||
| 68101 | shallow freshwater patch of a wetland which was the end of a freshwater creek region | 2017-09 | near Nagaon beach, Alibag | India | IND | Asia | 18.4417 | 72.9056 |
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 67770 | Methylococcaceae bacterium strain FWC3 16S ribosomal RNA gene, partial sequence | MH789551 | 1481 | ena | 1933926 |
| 67770 | Methylococcaceae bacterium strain FWC3 16S ribosomal RNA gene, partial sequence | MN080433 | 1527 | ena | 1933926 |
| 68101 | Methylococcaceae bacterium strain FWC3 16S ribosomal RNA gene, partial sequence | MN080433.1 | 1527 | ena | 1933926 |
Genome sequences
- @ref: 68101
- description: Methylococcaceae bacterium FWC3
- accession: GCA_004168015
- assembly level: scaffold
- database: ncbi
- NCBI tax ID: 2516328
GC content
- @ref: 67770
- GC-content: 63
- method: genome sequence analysis
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 89.157 | yes |
| flagellated | no | 94.308 | no |
| gram-positive | no | 98.698 | yes |
| anaerobic | no | 96.897 | yes |
| aerobic | yes | 84.789 | yes |
| halophile | no | 88.534 | no |
| spore-forming | no | 91.719 | no |
| glucose-util | yes | 64.568 | no |
| thermophile | no | 98.394 | no |
| glucose-ferment | no | 84.246 | no |
External links
@ref: 67770
culture collection no.: JCM 33786, KCTC 72733, MCC 4198, FWC 3
Reference
| @id | authors | title | doi/url | journal |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 68101 | Monali C. Rahalkar, Kumal Khatri, Jyoti Mohite, Pranitha S. Pandit, Rahul A. Bahulikar | A novel Type I methanotroph Methylolobus aquaticus gen. nov. sp. nov. isolated from a tropical wetland | 10.1007/s10482-020-01410-9 | Antonie Van Leeuwenhoek 113: 959-971 2020 |