Name: Streptomyces heilongjiangensis NEAU-W2
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 16024

Type strain:

Species: Streptomyces heilongjiangensis

Strain Designation: NEAU-W2

Culture col. no.: DSM 42073, CGMCC 4.7004, JCM 19675, ATCC BAA-2424

Strain history: CGMCC 4.7004 <-- C. Liu et al.; Northeast Agric. Univ., China; NEAU-W2.

NCBI tax ID(s): 945052 (species)

SI-ID 389433

ATCC BAA-2424, CGMCC 4.7004, DSM 42073

Special Collections

Section

Streptomyces heilongjiangensis NEAU-W2 is an aerobe, spore-forming, Gram-positive bacterium that builds an aerial mycelium and was isolated from root surface of soybean glycine max Merr.

Gram-positive
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Kitasatosporales*
	Family	*Streptomycetaceae*
	Genus	*Streptomyces*
	Species	**Streptomyces heilongjiangensis**
	Full Scientific Name (LPSN)	Streptomyces heilongjiangensis Liu et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30665]	Gram stain	positive

[Ref.: #30665]	Cell length	0.9 µm

[Ref.: #30665]	Cell width	0.7 µm

[Ref.: #30665]	Cell shape	rod-shaped

[Ref.: #30665]	Motility	no

[Ref.: #69339]	Colony color	Green brown (8000)
[Ref.: #69339]	Cultivation medium used	ISP 6

[Ref.: #69339]	Colony color	Colourless
[Ref.: #69339]	Cultivation medium used	ISP 2

[Ref.: #69339]	Colony color	Brown grey (7013), sepia brown (8014)
[Ref.: #69339]	Cultivation medium used	suter with tyrosine

[Ref.: #69339]	Colony color	Beige (1001)
[Ref.: #69339]	Cultivation medium used	ISP 5

[Ref.: #69339]	Colony color	Ivory (1014)
[Ref.: #69339]	Cultivation medium used	suter without tyrosine

[Ref.: #69339]	Colony color	Sand yellow (1002)
[Ref.: #69339]	Cultivation medium used	ISP 3

[Ref.: #69339]	Colony color	Stone grey ( 7030)
[Ref.: #69339]	Cultivation medium used	ISP 7

[Ref.: #69339]	Colony color	Yellow grey (7034)
[Ref.: #69339]	Cultivation medium used	ISP 4

[Ref.: #30665]	Pigment production	yes
[Ref.: #69339]	Pigment production	yes
[Ref.: #69339]	Pigment name	Melanin
[Ref.: #69339]	Pigment production	yes
[Ref.: #69339]	Pigmentation color	Sand yellow (1002), green brown (8000), stone grey (7030), olive grey (7002)
[Ref.: #69339]	Pigment name	soluble pigment

[Ref.: #69339]	Multicellular complex forming ability	no
[Ref.: #69339]	Multicellular complex name	Aerial mycelium
[Ref.: #69339]	Medium Name (multicellularity)	ISP 2

[Ref.: #69339]	Multicellular complex forming ability	yes
[Ref.: #69339]	Multicellular complex name	Aerial mycelium
[Ref.: #69339]	Multicellular complex color	Grey white (9002), signal white (9003)
[Ref.: #69339]	Medium Name (multicellularity)	ISP 3

[Ref.: #69339]	Multicellular complex forming ability	yes
[Ref.: #69339]	Multicellular complex name	Aerial mycelium
[Ref.: #69339]	Multicellular complex color	Traffic white (9016)
[Ref.: #69339]	Medium Name (multicellularity)	ISP 4

[Ref.: #69339]	Multicellular complex forming ability	yes
[Ref.: #69339]	Multicellular complex name	Aerial mycelium
[Ref.: #69339]	Multicellular complex color	Traffic white (9016)
[Ref.: #69339]	Medium Name (multicellularity)	ISP 5

[Ref.: #69339]	Multicellular complex forming ability	no
[Ref.: #69339]	Multicellular complex name	Aerial mycelium
[Ref.: #69339]	Medium Name (multicellularity)	ISP 6

[Ref.: #69339]	Multicellular complex forming ability	yes
[Ref.: #69339]	Multicellular complex name	Aerial mycelium
[Ref.: #69339]	Multicellular complex color	Signal white (9003)
[Ref.: #69339]	Medium Name (multicellularity)	ISP 7

[Ref.: #69339]	Multicellular complex forming ability	no
[Ref.: #69339]	Multicellular complex name	Aerial mycelium
[Ref.: #69339]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69339]	Multicellular complex forming ability	no
[Ref.: #69339]	Multicellular complex name	Aerial mycelium
[Ref.: #69339]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #18154]

Caption:

Medium 84 28°C

[Ref.: #18154]

Intellectual property rights:

[Ref.: #69339]

Caption:

Plates (252, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69339]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69339]

Caption:

Plates (252, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69339]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

[Ref.: #18154]

Culture medium

STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)

[Ref.: #18154]

Culture medium growth

[Ref.: #18154]

Culture medium link

Medium recipe at MediaDive

[Ref.: #18154]

Culture medium composition

expand / minimize

[Ref.: #18154]

Culture medium

ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)

[Ref.: #18154]

Culture medium growth

[Ref.: #18154]

Culture medium link

Medium recipe at MediaDive

[Ref.: #18154]

Culture medium composition

expand / minimize

[Ref.: #18154]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #18154]

Culture medium growth

[Ref.: #18154]

Culture medium link

Medium recipe at MediaDive

[Ref.: #18154]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #18154]	growth	28 °C
[Ref.: #30665]	growth	15-40 °C
[Ref.: #30665]	optimum	30 °C
[Ref.: #67770]	growth	28 °C

	pH	Kind of pH		pH
[Ref.: #30665]			optimum	7
[Ref.: #30665]			growth	05-11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30665]	Oxygen tolerance	aerobe
[Ref.: #69480]	Oxygen tolerance	aerobe Confidence in %90.425

[Ref.: #30665]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %92.962

[Ref.: #18154]

Name of produced compound

borrelidin

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30665]		NaCl		growth	0-5	%
[Ref.: #69339]		NaCl		growth	0-2.5	%

[Ref.: #30665]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-9(H₆), MK-9(H₈)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69339]	arabinose ChEBI	+	growth
[Ref.: #30665]	arabinose ChEBI	+	carbon source
[Ref.: #69339]	cellulose ChEBI	-	growth
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30665]	esculin ChEBI	+	hydrolysis
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #30665]	fructose ChEBI	+	carbon source
[Ref.: #69339]	fructose ChEBI	+	growth
[Ref.: #30665]	galactose ChEBI	+	carbon source
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #30665]	glucose ChEBI	+	carbon source
[Ref.: #69339]	glucose ChEBI	+	growth
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69339]	inositol ChEBI	+	growth
[Ref.: #30665]	lactose ChEBI	+	carbon source
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30665]	maltose ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30665]	mannitol ChEBI	+	carbon source
[Ref.: #30665]	mannose ChEBI	+	carbon source
[Ref.: #69339]	mannose ChEBI	+	growth
[Ref.: #30665]	nitrate ChEBI	+	reduction
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #30665]	raffinose ChEBI	+	carbon source
[Ref.: #69339]	raffinose ChEBI	+	growth
[Ref.: #30665]	rhamnose ChEBI	+	carbon source
[Ref.: #69339]	rhamnose ChEBI	+	growth
[Ref.: #30665]	ribose ChEBI	+	carbon source
[Ref.: #30665]	sorbitol ChEBI	+	carbon source
[Ref.: #30665]	sucrose ChEBI	+	carbon source
[Ref.: #69339]	sucrose ChEBI	+	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #30665]	xylose ChEBI	+	carbon source
[Ref.: #69339]	xylose ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #30665]		hydrogen sulfide ChEBI	yes

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68379]	alpha-glucosidase	-	3.2.1.20	* from API Coryne
[Ref.: #68379]	beta-galactosidase	+	3.2.1.23	* from API Coryne, API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69339]	3832	-	-	-	+	-	+	-	-	-	-	+	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69339]	6016	not determinedn.d.	+	+/-	+/-	-	+	+/-	-	+/-	+	+	+	+/-	+	-	+/-	+	+/-	+/-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #18154]	Sample type/isolated from	root surface of soybean glycine max (L.) Merr
[Ref.: #18154]	Host species	Glycine max
[Ref.: #18154]	Geographic location (country and/or sea, region)	Hulin, Heilongjiang province (46° 16' N 133° 36' E)
[Ref.: #18154]	Country	China
[Ref.: #18154]	Country ISO 3 Code	CHN
[Ref.: #18154]	Continent	Asia
[Ref.: #18154]	Geographic location	46.2667°/133.6000°

[Ref.: #67770]	Sample type/isolated from	Root surface of soybean from Hulin
[Ref.: #67770]	Geographic location (country and/or sea, region)	Heilongjiang Province, northern China
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18154]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #18154]	Streptomyces heilongjiangensis strain NEAU-W2 16S ribosomal RNA gene, partial sequence	JF431459	1520	GenBank ENA	945052 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Streptomyces heilongjiangensis NEAU-W2	GCA_028401405	contig	GenBank ENA	945052 tax ID	*

[Ref.: #18154]	GC-content	66.12 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #30665]	GC-content	66.12 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Streptomyces heilongjiangensis NEAU-W2 NCBI: GCA_028401405.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	88.774	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	90.487	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	90.425	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	92.962	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	94	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	89	yes

Availability in culture collections External links

[Ref.: #18154]

Culture collection no.

DSM 42073, CGMCC 4.7004, JCM 19675, ATCC BAA-2424

[Ref.: #85097]

SI-ID 389433

Literature:

	Title	Authors	Journal	DOI	Year
Phylogeny	Streptomyces heilongjiangensis sp. nov., a novel actinomycete that produces borrelidin isolated from the root surface of soybean [Glycine max (L.) Merr].	Liu C, Wang X, Yan Y, Wang J, Zhang B, Zhang J, Xiang W	Int J Syst Evol Microbiol	10.1099/ijs.0.041483-0	2012	*
	Discovery of Three 22-Membered Macrolides by Deciphering the Streamlined Genome of Mangrove-Derived Streptomyces sp. HM190.	Ye Y, Anwar N, Mao X, Wu S, Yan C, Zhao Z, Zhang R, Nie Y, Zhang J, Wang J, Wu M	Front Microbiol	10.3389/fmicb.2020.01464	2020	*

References

#18154

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 42073

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30665

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26996 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69339

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#85097

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID389433.1 )

#26996

IJSEM 1030 2013 ( DOI 10.1099/ijs.0.041483-0 , PubMed 22707527 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Streptomyces heilongjiangensis in external resources:
SILVA
BRENDA
PANGAEA
GBIF