Strain identifier

BacDive ID: 16024

Type strain: Yes

Species: Streptomyces heilongjiangensis

Strain Designation: NEAU-W2

Strain history: CGMCC 4.7004 <-- C. Liu et al.; Northeast Agric. Univ., China; NEAU-W2.

NCBI tax ID(s): 945052 (species)

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General

@ref: 18154

BacDive-ID: 16024

DSM-Number: 42073

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, rod-shaped

description: Streptomyces heilongjiangensis NEAU-W2 is an aerobe, spore-forming, Gram-positive bacterium that builds an aerial mycelium and was isolated from root surface of soybean glycine max Merr.

NCBI tax id

  • NCBI tax id: 945052
  • Matching level: species

strain history

@refhistory
18154<- C. Liu,School of Life Science, Northeast Agricultural University, China; NEAU-W2
67770CGMCC 4.7004 <-- C. Liu et al.; Northeast Agric. Univ., China; NEAU-W2.

doi: 10.13145/bacdive16024.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces heilongjiangensis
  • full scientific name: Streptomyces heilongjiangensis Liu et al. 2013

@ref: 18154

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces heilongjiangensis

full scientific name: Streptomyces heilongjiangensis Liu et al. 2013

strain designation: NEAU-W2

type strain: yes

Morphology

cell morphology

  • @ref: 30665
  • gram stain: positive
  • cell length: 0.9 µm
  • cell width: 0.7 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colormedium used
69339Green brown (8000)ISP 6
69339ColourlessISP 2
69339Brown grey (7013), sepia brown (8014)suter with tyrosine
69339Beige (1001)ISP 5
69339Ivory (1014)suter without tyrosine
69339Sand yellow (1002)ISP 3
69339Stone grey ( 7030)ISP 7
69339Yellow grey (7034)ISP 4

multicellular morphology

@refforms multicellular complexcomplex namemedium namecomplex color
69339noAerial myceliumISP 2
69339yesAerial myceliumISP 3Grey white (9002), signal white (9003)
69339yesAerial myceliumISP 4Traffic white (9016)
69339yesAerial myceliumISP 5Traffic white (9016)
69339noAerial myceliumISP 6
69339yesAerial myceliumISP 7Signal white (9003)
69339noAerial myceliumsuter with tyrosine
69339noAerial myceliumsuter without tyrosine

pigmentation

@refproductionnamecolor
30665yes
69339yesMelanin
69339yessoluble pigmentSand yellow (1002), green brown (8000), stone grey (7030), olive grey (7002)

multimedia

@refmultimedia contentcaptionintellectual property rights
18154https://www.dsmz.de/microorganisms/photos/DSM_42073.jpgMedium 84 28°C© Leibniz-Institut DSMZ
69339DSM_42073_image3.jpegPlates (252, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69339DSM_42073_image4.jpegPlates (252, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
18154STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
18154ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
18154GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperature
18154positivegrowth28
30665positivegrowth15-40
30665positiveoptimum30
67770positivegrowth28

culture pH

@refabilitytypepHPH range
30665positivegrowth05-11alkaliphile
30665positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
30665aerobe
69480aerobe90.425

spore formation

@refspore formationconfidence
30665yes
69480yes92.962

compound production

  • @ref: 18154
  • compound: borrelidin

halophily

@refsaltgrowthtested relationconcentration
30665NaClpositivegrowth0-5 %
69339NaClpositivegrowth0-2.5 %

observation

@refobservation
30665aggregates in chains
67770quinones: MK-9(H6), MK-9(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6933922599arabinose+growth
3066522599arabinose+carbon source
3066528757fructose+carbon source
3066528260galactose+carbon source
3066517234glucose+carbon source
3066517716lactose+carbon source
3066517306maltose+carbon source
3066529864mannitol+carbon source
3066537684mannose+carbon source
3066516634raffinose+carbon source
3066526546rhamnose+carbon source
3066533942ribose+carbon source
3066530911sorbitol+carbon source
3066517992sucrose+carbon source
3066518222xylose+carbon source
306654853esculin+hydrolysis
3066517632nitrate+reduction
6933962968cellulose-growth
6933928757fructose+growth
6933917234glucose+growth
6933917268inositol+growth
6933937684mannose+growth
6933916634raffinose+growth
6933926546rhamnose+growth
6933917992sucrose+growth
6933918222xylose+growth
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 30665
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: yes

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382cystine arylamidase-3.4.11.3
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382beta-glucosidase+3.2.1.21
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69339---+-+----+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69339++/-+/--++/--+/-++++/-+-+/-++/-+/--

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentlatitudelongitude
18154root surface of soybean glycine max (L.) MerrGlycine maxHulin, Heilongjiang province (46° 16' N 133° 36' E)ChinaCHNAsia46.2667133.6
67770Root surface of soybean from HulinHeilongjiang Province, northern ChinaChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Root (Rhizome)

Safety information

risk assessment

  • @ref: 18154
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 18154
  • description: Streptomyces heilongjiangensis strain NEAU-W2 16S ribosomal RNA gene, partial sequence
  • accession: JF431459
  • length: 1520
  • database: nuccore
  • NCBI tax ID: 945052

Genome sequences

  • @ref: 66792
  • description: Streptomyces heilongjiangensis NEAU-W2
  • accession: GCA_028401405
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 945052

GC content

@refGC-contentmethod
1815466.12thermal denaturation, midpoint method (Tm)
3066566.12

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingyes88.774yes
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no90.487no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes90.425yes
69480spore-formingspore-formingAbility to form endo- or exosporesyes92.962yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno94no
69480flagellatedmotile2+Ability to perform flagellated movementno89yes

External links

@ref: 18154

culture collection no.: DSM 42073, CGMCC 4.7004, JCM 19675, ATCC BAA-2424

straininfo link

  • @ref: 85097
  • straininfo: 389433

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22707527Streptomyces heilongjiangensis sp. nov., a novel actinomycete that produces borrelidin isolated from the root surface of soybean [Glycine max (L.) Merr].Liu C, Wang X, Yan Y, Wang J, Zhang B, Zhang J, Xiang WInt J Syst Evol Microbiol10.1099/ijs.0.041483-02012Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Fatty Alcohols/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, *Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil Microbiology, Soybeans/*microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analysisGenetics
32676068Discovery of Three 22-Membered Macrolides by Deciphering the Streamlined Genome of Mangrove-Derived Streptomyces sp. HM190.Ye Y, Anwar N, Mao X, Wu S, Yan C, Zhao Z, Zhang R, Nie Y, Zhang J, Wang J, Wu MFront Microbiol10.3389/fmicb.2020.014642020

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
18154Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 42073)https://www.dsmz.de/collection/catalogue/details/culture/DSM-42073
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30665Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2699628776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69339Wink, J.https://cdn.dsmz.de/wink/DSM%2042073.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
85097Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID389433.1StrainInfo: A central database for resolving microbial strain identifiers