Strain identifier

BacDive ID: 16024

Type strain: Yes

Species: Streptomyces heilongjiangensis

Strain Designation: NEAU-W2

Strain history: CGMCC 4.7004 <-- C. Liu et al.; Northeast Agric. Univ., China; NEAU-W2.

NCBI tax ID(s): 945052 (species)

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General

@ref: 18154

BacDive-ID: 16024

DSM-Number: 42073

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, rod-shaped

description: Streptomyces heilongjiangensis NEAU-W2 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from root surface of soybean glycine max Merr.

NCBI tax id

  • NCBI tax id: 945052
  • Matching level: species

strain history

@refhistory
18154<- C. Liu,School of Life Science, Northeast Agricultural University, China; NEAU-W2
67770CGMCC 4.7004 <-- C. Liu et al.; Northeast Agric. Univ., China; NEAU-W2.

doi: 10.13145/bacdive16024.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces heilongjiangensis
  • full scientific name: Streptomyces heilongjiangensis Liu et al. 2013

@ref: 18154

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces heilongjiangensis

full scientific name: Streptomyces heilongjiangensis Liu et al. 2013

strain designation: NEAU-W2

type strain: yes

Morphology

cell morphology

  • @ref: 30665
  • gram stain: positive
  • cell length: 0.9 µm
  • cell width: 0.7 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colormedium used
69339Green brown (8000)ISP 6
69339ColourlessISP 2
69339Brown grey (7013), sepia brown (8014)suter with tyrosine
69339Beige (1001)ISP 5
69339Ivory (1014)suter without tyrosine
69339Sand yellow (1002)ISP 3
69339Stone grey ( 7030)ISP 7
69339Yellow grey (7034)ISP 4

multicellular morphology

@refforms multicellular complexcomplex namemedium namecomplex color
69339noAerial myceliumISP 2
69339yesAerial myceliumISP 3Grey white (9002), signal white (9003)
69339yesAerial myceliumISP 4Traffic white (9016)
69339yesAerial myceliumISP 5Traffic white (9016)
69339noAerial myceliumISP 6
69339yesAerial myceliumISP 7Signal white (9003)
69339noAerial myceliumsuter with tyrosine
69339noAerial myceliumsuter without tyrosine

pigmentation

@refproductionnamecolor
30665yes
69339yesMelanin
69339yessoluble pigmentSand yellow (1002), green brown (8000), stone grey (7030), olive grey (7002)

multimedia

@refmultimedia contentcaptionintellectual property rights
18154https://www.dsmz.de/microorganisms/photos/DSM_42073.jpgMedium 84 28°C© Leibniz-Institut DSMZ
69339DSM_42073_image3.jpegPlates (252, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69339DSM_42073_image4.jpegPlates (252, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
18154STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
18154ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
18154GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
18154positivegrowth28mesophilic
30665positivegrowth15-40
30665positiveoptimum30mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
30665positivegrowth05-11alkaliphile
30665positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 30665
  • oxygen tolerance: aerobe

spore formation

  • @ref: 30665
  • spore formation: yes

compound production

  • @ref: 18154
  • compound: borrelidin

halophily

@refsaltgrowthtested relationconcentration
30665NaClpositivegrowth0-5 %
69339NaClpositivegrowth0-2.5 %

observation

@refobservation
30665aggregates in chains
67770quinones: MK-9(H6), MK-9(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6933922599arabinose+growth
3066522599arabinose+carbon source
3066528757fructose+carbon source
3066528260galactose+carbon source
3066517234glucose+carbon source
3066517716lactose+carbon source
3066517306maltose+carbon source
3066529864mannitol+carbon source
3066537684mannose+carbon source
3066516634raffinose+carbon source
3066526546rhamnose+carbon source
3066533942ribose+carbon source
3066530911sorbitol+carbon source
3066517992sucrose+carbon source
3066518222xylose+carbon source
306654853esculin+hydrolysis
3066517632nitrate+reduction
6933962968cellulose-growth
6933928757fructose+growth
6933917234glucose+growth
6933917268inositol+growth
6933937684mannose+growth
6933916634raffinose+growth
6933926546rhamnose+growth
6933917992sucrose+growth
6933918222xylose+growth
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 30665
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: yes

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382cystine arylamidase-3.4.11.3
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382beta-glucosidase+3.2.1.21
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69339---+-+----+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69339++/-+/--++/--+/-++++/-+-+/-++/-+/--

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentlatitudelongitude
18154root surface of soybean glycine max (L.) MerrGlycine maxHulin, Heilongjiang province (46° 16' N 133° 36' E)ChinaCHNAsia46.2667133.6
67770Root surface of soybean from HulinHeilongjiang Province, northern ChinaChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Root (Rhizome)

Safety information

risk assessment

  • @ref: 18154
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 18154
  • description: Streptomyces heilongjiangensis strain NEAU-W2 16S ribosomal RNA gene, partial sequence
  • accession: JF431459
  • length: 1520
  • database: ena
  • NCBI tax ID: 945052

Genome sequences

  • @ref: 66792
  • description: Streptomyces heilongjiangensis NEAU-W2
  • accession: GCA_028401405
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 945052

GC content

@refGC-contentmethod
1815466.12thermal denaturation, midpoint method (Tm)
3066566.12

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno95.039yes
flagellatedno98.082yes
gram-positiveyes88.547yes
anaerobicno98.857yes
aerobicyes91.704yes
halophileno91.927no
spore-formingyes96.079yes
glucose-fermentno88.251no
thermophileno98.073yes
glucose-utilyes90.171no

External links

@ref: 18154

culture collection no.: DSM 42073, CGMCC 4.7004, JCM 19675, ATCC BAA-2424

straininfo link

  • @ref: 85097
  • straininfo: 389433

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22707527Streptomyces heilongjiangensis sp. nov., a novel actinomycete that produces borrelidin isolated from the root surface of soybean [Glycine max (L.) Merr].Liu C, Wang X, Yan Y, Wang J, Zhang B, Zhang J, Xiang WInt J Syst Evol Microbiol10.1099/ijs.0.041483-02012Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Fatty Alcohols/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, *Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil Microbiology, Soybeans/*microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analysisGenetics
32676068Discovery of Three 22-Membered Macrolides by Deciphering the Streamlined Genome of Mangrove-Derived Streptomyces sp. HM190.Ye Y, Anwar N, Mao X, Wu S, Yan C, Zhao Z, Zhang R, Nie Y, Zhang J, Wang J, Wu MFront Microbiol10.3389/fmicb.2020.014642020

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
18154Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 42073)https://www.dsmz.de/collection/catalogue/details/culture/DSM-42073
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30665Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2699628776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69339Wink, J.https://cdn.dsmz.de/wink/DSM%2042073.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
85097Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID389433.1StrainInfo: A central database for resolving microbial strain identifiers